Intratracheal inoculation of severe acute respiratory syndrome coronavirus in monkeys Macaca rhesus.
Identifieur interne : 001C56 ( PubMed/Corpus ); précédent : 001C55; suivant : 001C57Intratracheal inoculation of severe acute respiratory syndrome coronavirus in monkeys Macaca rhesus.
Auteurs : F. Luo ; W. Hou ; Z Q Yang ; Z J Tang ; Y. Wang ; Q Y Xian ; L H SunSource :
- Acta virologica [ 0001-723X ] ; 2007.
English descriptors
- KwdEn :
- Animals, Antigens, Viral (analysis), Blood (virology), Bodily Secretions (virology), Disease Models, Animal, Feces (virology), Fever (virology), Fluorescent Antibody Technique, Direct, Liver (pathology), Liver (virology), Lung (pathology), Lung (virology), Macaca mulatta, RNA, Viral (analysis), Radiography, Thoracic, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (growth & development), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (physiopathology), Time Factors, Urine (virology).
- MESH :
- chemical , analysis : Antigens, Viral, RNA, Viral.
- growth & development : SARS Virus.
- isolation & purification : SARS Virus.
- pathology : Liver, Lung, Severe Acute Respiratory Syndrome.
- physiopathology : Severe Acute Respiratory Syndrome.
- virology : Blood, Bodily Secretions, Feces, Fever, Liver, Lung, Urine.
- Animals, Disease Models, Animal, Fluorescent Antibody Technique, Direct, Macaca mulatta, Radiography, Thoracic, Reverse Transcriptase Polymerase Chain Reaction, Time Factors.
Abstract
An animal model for infection with Severe acute respiratory syndrome coronavirus (SARSCoV) was evaluated in monkeys Macaca rhesus. The monkeys were inoculated into the trachea with NS-I strain of SARS-CoV and the clinical manifestation of the illness was monitored. The clinical samples collected from infected monkeys were examined by immumnofluorescence assay (IFA), pathological inspection, RTPCR, and by virus isolation. The infected animals demonstrated mild clinical symptoms including fever. Two of the six infected monkeys developed fever (1.5 above the level before challenge) on the day 10 post inoculation (p.i.). Although the severe clinical symptoms or mortality were not observed, the virological and histopathological evidences of the illness were evident. The specimens collected from the infected animals showed the presence of SARS-CoV detected by RT-PCR, IFA, and by virus isolation. From the organs examined postmortem, a major pathological change was observed in the lungs. The walls of the alveoli were thicker, infiltrated with inflammation cells and an exudative fluid was found in the alveolar spaces. In addition, some alveolar spaces showed hyaline membrane lining. The results showed that the monkeys infected with SARS-CoV developed the typical SARS according to clinical, virological, and pathological findings.
PubMed: 18076307
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pubmed:18076307Le document en format XML
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Wang</name></author><author><name sortKey="Xian, Q Y" sort="Xian, Q Y" uniqKey="Xian Q" first="Q Y" last="Xian">Q Y Xian</name></author><author><name sortKey="Sun, L H" sort="Sun, L H" uniqKey="Sun L" first="L H" last="Sun">L H Sun</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:18076307</idno><idno type="pmid">18076307</idno><idno type="wicri:Area/PubMed/Corpus">001C56</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Intratracheal inoculation of severe acute respiratory syndrome coronavirus in monkeys Macaca rhesus.</title><author><name sortKey="Luo, F" sort="Luo, F" uniqKey="Luo F" first="F" last="Luo">F. Luo</name><affiliation><nlm:affiliation>Institue of Virology, Wuhan University School of Medicine, Wuhan, China.</nlm:affiliation></affiliation></author><author><name sortKey="Hou, W" sort="Hou, W" uniqKey="Hou W" first="W" last="Hou">W. Hou</name></author><author><name sortKey="Yang, Z Q" sort="Yang, Z Q" uniqKey="Yang Z" first="Z Q" last="Yang">Z Q Yang</name></author><author><name sortKey="Tang, Z J" sort="Tang, Z J" uniqKey="Tang Z" first="Z J" last="Tang">Z J Tang</name></author><author><name sortKey="Wang, Y" sort="Wang, Y" uniqKey="Wang Y" first="Y" last="Wang">Y. Wang</name></author><author><name sortKey="Xian, Q Y" sort="Xian, Q Y" uniqKey="Xian Q" first="Q Y" last="Xian">Q Y Xian</name></author><author><name sortKey="Sun, L H" sort="Sun, L H" uniqKey="Sun L" first="L H" last="Sun">L H Sun</name></author></analytic><series><title level="j">Acta virologica</title><idno type="ISSN">0001-723X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Viral (analysis)</term><term>Blood (virology)</term><term>Bodily Secretions (virology)</term><term>Disease Models, Animal</term><term>Feces (virology)</term><term>Fever (virology)</term><term>Fluorescent Antibody Technique, Direct</term><term>Liver (pathology)</term><term>Liver (virology)</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Macaca mulatta</term><term>RNA, Viral (analysis)</term><term>Radiography, Thoracic</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (growth & development)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe Acute Respiratory Syndrome (physiopathology)</term><term>Time Factors</term><term>Urine (virology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Liver</term><term>Lung</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Blood</term><term>Bodily Secretions</term><term>Feces</term><term>Fever</term><term>Liver</term><term>Lung</term><term>Urine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Fluorescent Antibody Technique, Direct</term><term>Macaca mulatta</term><term>Radiography, Thoracic</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An animal model for infection with Severe acute respiratory syndrome coronavirus (SARSCoV) was evaluated in monkeys Macaca rhesus. The monkeys were inoculated into the trachea with NS-I strain of SARS-CoV and the clinical manifestation of the illness was monitored. The clinical samples collected from infected monkeys were examined by immumnofluorescence assay (IFA), pathological inspection, RTPCR, and by virus isolation. The infected animals demonstrated mild clinical symptoms including fever. Two of the six infected monkeys developed fever (1.5 above the level before challenge) on the day 10 post inoculation (p.i.). Although the severe clinical symptoms or mortality were not observed, the virological and histopathological evidences of the illness were evident. The specimens collected from the infected animals showed the presence of SARS-CoV detected by RT-PCR, IFA, and by virus isolation. From the organs examined postmortem, a major pathological change was observed in the lungs. The walls of the alveoli were thicker, infiltrated with inflammation cells and an exudative fluid was found in the alveolar spaces. In addition, some alveolar spaces showed hyaline membrane lining. The results showed that the monkeys infected with SARS-CoV developed the typical SARS according to clinical, virological, and pathological findings.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18076307</PMID><DateCompleted><Year>2008</Year><Month>01</Month><Day>10</Day></DateCompleted><DateRevised><Year>2007</Year><Month>12</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-723X</ISSN><JournalIssue CitedMedium="Print"><Volume>51</Volume><Issue>3</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>Acta virologica</Title><ISOAbbreviation>Acta Virol.</ISOAbbreviation></Journal><ArticleTitle>Intratracheal inoculation of severe acute respiratory syndrome coronavirus in monkeys Macaca rhesus.</ArticleTitle><Pagination><MedlinePgn>171-7</MedlinePgn></Pagination><Abstract><AbstractText>An animal model for infection with Severe acute respiratory syndrome coronavirus (SARSCoV) was evaluated in monkeys Macaca rhesus. The monkeys were inoculated into the trachea with NS-I strain of SARS-CoV and the clinical manifestation of the illness was monitored. The clinical samples collected from infected monkeys were examined by immumnofluorescence assay (IFA), pathological inspection, RTPCR, and by virus isolation. The infected animals demonstrated mild clinical symptoms including fever. Two of the six infected monkeys developed fever (1.5 above the level before challenge) on the day 10 post inoculation (p.i.). Although the severe clinical symptoms or mortality were not observed, the virological and histopathological evidences of the illness were evident. The specimens collected from the infected animals showed the presence of SARS-CoV detected by RT-PCR, IFA, and by virus isolation. From the organs examined postmortem, a major pathological change was observed in the lungs. The walls of the alveoli were thicker, infiltrated with inflammation cells and an exudative fluid was found in the alveolar spaces. In addition, some alveolar spaces showed hyaline membrane lining. The results showed that the monkeys infected with SARS-CoV developed the typical SARS according to clinical, virological, and pathological findings.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institue of Virology, Wuhan University School of Medicine, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Z Q</ForeName><Initials>ZQ</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Z J</ForeName><Initials>ZJ</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Xian</LastName><ForeName>Q Y</ForeName><Initials>QY</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>L H</ForeName><Initials>LH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Slovakia</Country><MedlineTA>Acta Virol</MedlineTA><NlmUniqueID>0370401</NlmUniqueID><ISSNLinking>0001-723X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001769" MajorTopicYN="N">Blood</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012634" MajorTopicYN="N">Bodily Secretions</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004195" MajorTopicYN="Y">Disease Models, Animal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005243" MajorTopicYN="N">Feces</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005334" MajorTopicYN="N">Fever</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019085" MajorTopicYN="N">Fluorescent Antibody Technique, Direct</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008099" MajorTopicYN="N">Liver</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008253" MajorTopicYN="N">Macaca mulatta</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013902" MajorTopicYN="N">Radiography, Thoracic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" 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