Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA.
Identifieur interne : 001A43 ( PubMed/Corpus ); précédent : 001A42; suivant : 001A44Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA.
Auteurs : Hyun-Kyoung Lee ; Byoung-Hee Lee ; Noton Kumar Dutta ; Seung-Hyeok Seok ; Min-Won Baek ; Hui-Young Lee ; Dong-Jae Kim ; Yi-Rang Na ; Kyoung-Jin Noh ; Sung-Hoon Park ; Hiroaki Kariwa ; Mina Nakauchi ; Le Quynh Mai ; Suk-Jin Heo ; Jae-Hak ParkSource :
- Journal of microbiology and biotechnology [ 1017-7825 ] ; 2008.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Antigens, Viral (immunology), Antigens, Viral (isolation & purification), Antigens, Viral (metabolism), Enzyme-Linked Immunosorbent Assay (methods), Gene Expression, Humans, Nucleocapsid (immunology), Nucleocapsid (isolation & purification), Nucleocapsid (metabolism), Recombinant Proteins (immunology), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Antigens, Viral, Recombinant Proteins.
- chemical , isolation & purification : Antigens, Viral, Recombinant Proteins.
- chemical , metabolism : Antigens, Viral, Recombinant Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : Nucleocapsid, SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : Nucleocapsid.
- metabolism : Nucleocapsid.
- methods : Enzyme-Linked Immunosorbent Assay.
- Gene Expression, Humans.
Abstract
Severe acute respiratory syndrome (SARS) is a lifethreatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1- 109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.
PubMed: 18955825
Links to Exploration step
pubmed:18955825Le document en format XML
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last="Nakauchi">Mina Nakauchi</name></author><author><name sortKey="Mai, Le Quynh" sort="Mai, Le Quynh" uniqKey="Mai L" first="Le Quynh" last="Mai">Le Quynh Mai</name></author><author><name sortKey="Heo, Suk Jin" sort="Heo, Suk Jin" uniqKey="Heo S" first="Suk-Jin" last="Heo">Suk-Jin Heo</name></author><author><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18955825</idno><idno type="pmid">18955825</idno><idno type="wicri:Area/PubMed/Corpus">001A43</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA.</title><author><name sortKey="Lee, Hyun Kyoung" sort="Lee, Hyun Kyoung" uniqKey="Lee H" first="Hyun-Kyoung" last="Lee">Hyun-Kyoung Lee</name><affiliation><nlm:affiliation>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name></author><author><name sortKey="Dutta, Noton Kumar" sort="Dutta, Noton Kumar" uniqKey="Dutta N" first="Noton Kumar" last="Dutta">Noton Kumar Dutta</name></author><author><name sortKey="Seok, Seung Hyeok" sort="Seok, Seung Hyeok" uniqKey="Seok S" first="Seung-Hyeok" last="Seok">Seung-Hyeok Seok</name></author><author><name sortKey="Baek, Min Won" sort="Baek, Min Won" uniqKey="Baek M" first="Min-Won" last="Baek">Min-Won Baek</name></author><author><name sortKey="Lee, Hui Young" sort="Lee, Hui Young" uniqKey="Lee H" first="Hui-Young" last="Lee">Hui-Young Lee</name></author><author><name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name></author><author><name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name></author><author><name sortKey="Noh, Kyoung Jin" sort="Noh, Kyoung Jin" uniqKey="Noh K" first="Kyoung-Jin" last="Noh">Kyoung-Jin Noh</name></author><author><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name></author><author><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name></author><author><name sortKey="Nakauchi, Mina" sort="Nakauchi, Mina" uniqKey="Nakauchi M" first="Mina" last="Nakauchi">Mina Nakauchi</name></author><author><name sortKey="Mai, Le Quynh" sort="Mai, Le Quynh" uniqKey="Mai L" first="Le Quynh" last="Mai">Le Quynh Mai</name></author><author><name sortKey="Heo, Suk Jin" sort="Heo, Suk Jin" uniqKey="Heo S" first="Suk-Jin" last="Heo">Suk-Jin Heo</name></author><author><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name></author></analytic><series><title level="j">Journal of microbiology and biotechnology</title><idno type="ISSN">1017-7825</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Antigens, Viral (immunology)</term><term>Antigens, Viral (isolation & purification)</term><term>Antigens, Viral (metabolism)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Gene Expression</term><term>Humans</term><term>Nucleocapsid (immunology)</term><term>Nucleocapsid (isolation & purification)</term><term>Nucleocapsid (metabolism)</term><term>Recombinant Proteins (immunology)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antigens, Viral</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Viral</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Nucleocapsid</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a lifethreatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1- 109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18955825</PMID><DateCompleted><Year>2009</Year><Month>06</Month><Day>08</Day></DateCompleted><DateRevised><Year>2008</Year><Month>10</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1017-7825</ISSN><JournalIssue CitedMedium="Print"><Volume>18</Volume><Issue>10</Issue><PubDate><Year>2008</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of microbiology and biotechnology</Title><ISOAbbreviation>J. Microbiol. Biotechnol.</ISOAbbreviation></Journal><ArticleTitle>Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA.</ArticleTitle><Pagination><MedlinePgn>1717-21</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is a lifethreatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1- 109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyun-Kyoung</ForeName><Initials>HK</Initials><AffiliationInfo><Affiliation>Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Byoung-Hee</ForeName><Initials>BH</Initials></Author><Author ValidYN="Y"><LastName>Dutta</LastName><ForeName>Noton Kumar</ForeName><Initials>NK</Initials></Author><Author ValidYN="Y"><LastName>Seok</LastName><ForeName>Seung-Hyeok</ForeName><Initials>SH</Initials></Author><Author ValidYN="Y"><LastName>Baek</LastName><ForeName>Min-Won</ForeName><Initials>MW</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hui-Young</ForeName><Initials>HY</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Dong-Jae</ForeName><Initials>DJ</Initials></Author><Author ValidYN="Y"><LastName>Na</LastName><ForeName>Yi-Rang</ForeName><Initials>YR</Initials></Author><Author ValidYN="Y"><LastName>Noh</LastName><ForeName>Kyoung-Jin</ForeName><Initials>KJ</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Sung-Hoon</ForeName><Initials>SH</Initials></Author><Author ValidYN="Y"><LastName>Kariwa</LastName><ForeName>Hiroaki</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Nakauchi</LastName><ForeName>Mina</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Mai</LastName><ForeName>Le Quynh</ForeName><Initials>le Q</Initials></Author><Author ValidYN="Y"><LastName>Heo</LastName><ForeName>Suk-Jin</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Jae-Hak</ForeName><Initials>JH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>J Microbiol Biotechnol</MedlineTA><NlmUniqueID>9431852</NlmUniqueID><ISSNLinking>1017-7825</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019251" MajorTopicYN="N">Nucleocapsid</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>6</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18955825</ArticleId><ArticleId IdType="pii">8112</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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