Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays.
Identifieur interne : 001A27 ( PubMed/Corpus ); précédent : 001A26; suivant : 001A28Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays.
Auteurs : R Y T. Sung ; Paul K S. Chan ; Tracy Tsen ; A M Li ; W Y Lam ; Apple C M. Yeung ; E A S. NelsonSource :
- Journal of medical virology [ 1096-9071 ] ; 2009.
English descriptors
- KwdEn :
- Bacteria (genetics), Bacteria (isolation & purification), Bacterial Infections (diagnosis), Bacterial Infections (epidemiology), Bacterial Infections (microbiology), Child, Preschool, Exudates and Transudates (microbiology), Exudates and Transudates (virology), Female, Hong Kong (epidemiology), Humans, Infant, Infant, Newborn, Male, Polymerase Chain Reaction (methods), Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (etiology), Seasons, Virus Diseases (diagnosis), Virus Diseases (epidemiology), Virus Diseases (virology), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- geographic , epidemiology : Hong Kong.
- diagnosis : Bacterial Infections, Virus Diseases.
- epidemiology : Bacterial Infections, Respiratory Tract Infections, Virus Diseases.
- etiology : Respiratory Tract Infections.
- genetics : Bacteria, Viruses.
- isolation & purification : Bacteria, Viruses.
- methods : Polymerase Chain Reaction.
- microbiology : Bacterial Infections, Exudates and Transudates.
- virology : Exudates and Transudates, Virus Diseases.
- Child, Preschool, Female, Humans, Infant, Infant, Newborn, Male, Seasons.
Abstract
Acute respiratory tract infection is a leading cause of hospital admission of children. This study used a broad capture, rapid and sensitive method (multiplex PCR assay) to detect 20 different respiratory pathogens including influenza A subtypes H1, H3, and H5; influenza B; parainfluenza types 1, 2, 3, and 4; respiratory syncytial virus (RSV) groups A and B; adenoviruses; human rhinoviruses; enteroviruses; human metapneumoviruses; human coronaviruses OC43, 229E, and SARS-CoV; Chlamydophila pneumoniae; Legionella pneumophila; and Mycoplasma pneumoniae; from respiratory specimens of 475 children hospitalized over a 12-month period for acute respiratory tract infections. The overall positive rate (47%) was about twice higher than previous reports based on conventional methods. Influenza A, parainfluenza and RSV accounted for 51%, and non-cultivable viruses accounted for 30% of positive cases. Influenza A peaked at March and June. Influenza B was detected in January, February, and April. Parainfluenza was prevalent throughout the year except from April to June. Most RSV infections were found between February and September. Adenovirus had multiple peaks, whereas rhinovirus and coronavirus OC43 were detected mainly in winter and early spring. RSV infection was associated with bronchiolitis, and parainfluenza was associated with croup; otherwise the clinical manifestations were largely nonspecific. In general, children infected with influenza A, adenovirus and mixed viruses had higher temperatures. In view of the increasing concern about unexpected outbreaks of severe viral infections, a rapid multiplex PCR assay is a valuable tool to enhance the management of hospitalized patients, and for the surveillance for viral infections circulating in the community.
DOI: 10.1002/jmv.21364
PubMed: 19031443
Links to Exploration step
pubmed:19031443Le document en format XML
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Chan</name></author><author><name sortKey="Tsen, Tracy" sort="Tsen, Tracy" uniqKey="Tsen T" first="Tracy" last="Tsen">Tracy Tsen</name></author><author><name sortKey="Li, A M" sort="Li, A M" uniqKey="Li A" first="A M" last="Li">A M Li</name></author><author><name sortKey="Lam, W Y" sort="Lam, W Y" uniqKey="Lam W" first="W Y" last="Lam">W Y Lam</name></author><author><name sortKey="Yeung, Apple C M" sort="Yeung, Apple C M" uniqKey="Yeung A" first="Apple C M" last="Yeung">Apple C M. Yeung</name></author><author><name sortKey="Nelson, E A S" sort="Nelson, E A S" uniqKey="Nelson E" first="E A S" last="Nelson">E A S. 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Sung</name><affiliation><nlm:affiliation>Department of Paediatrics, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></author><author><name sortKey="Tsen, Tracy" sort="Tsen, Tracy" uniqKey="Tsen T" first="Tracy" last="Tsen">Tracy Tsen</name></author><author><name sortKey="Li, A M" sort="Li, A M" uniqKey="Li A" first="A M" last="Li">A M Li</name></author><author><name sortKey="Lam, W Y" sort="Lam, W Y" uniqKey="Lam W" first="W Y" last="Lam">W Y Lam</name></author><author><name sortKey="Yeung, Apple C M" sort="Yeung, Apple C M" uniqKey="Yeung A" first="Apple C M" last="Yeung">Apple C M. Yeung</name></author><author><name sortKey="Nelson, E A S" sort="Nelson, E A S" uniqKey="Nelson E" first="E A S" last="Nelson">E A S. Nelson</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="eISSN">1096-9071</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>Bacterial Infections (diagnosis)</term><term>Bacterial Infections (epidemiology)</term><term>Bacterial Infections (microbiology)</term><term>Child, Preschool</term><term>Exudates and Transudates (microbiology)</term><term>Exudates and Transudates (virology)</term><term>Female</term><term>Hong Kong (epidemiology)</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Polymerase Chain Reaction (methods)</term><term>Respiratory Tract Infections (epidemiology)</term><term>Respiratory Tract Infections (etiology)</term><term>Seasons</term><term>Virus Diseases (diagnosis)</term><term>Virus Diseases (epidemiology)</term><term>Virus Diseases (virology)</term><term>Viruses (genetics)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Hong Kong</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Bacterial Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Bacterial Infections</term><term>Respiratory Tract Infections</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Bacterial Infections</term><term>Exudates and Transudates</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Exudates and Transudates</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Male</term><term>Seasons</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Acute respiratory tract infection is a leading cause of hospital admission of children. This study used a broad capture, rapid and sensitive method (multiplex PCR assay) to detect 20 different respiratory pathogens including influenza A subtypes H1, H3, and H5; influenza B; parainfluenza types 1, 2, 3, and 4; respiratory syncytial virus (RSV) groups A and B; adenoviruses; human rhinoviruses; enteroviruses; human metapneumoviruses; human coronaviruses OC43, 229E, and SARS-CoV; Chlamydophila pneumoniae; Legionella pneumophila; and Mycoplasma pneumoniae; from respiratory specimens of 475 children hospitalized over a 12-month period for acute respiratory tract infections. The overall positive rate (47%) was about twice higher than previous reports based on conventional methods. Influenza A, parainfluenza and RSV accounted for 51%, and non-cultivable viruses accounted for 30% of positive cases. Influenza A peaked at March and June. Influenza B was detected in January, February, and April. Parainfluenza was prevalent throughout the year except from April to June. Most RSV infections were found between February and September. Adenovirus had multiple peaks, whereas rhinovirus and coronavirus OC43 were detected mainly in winter and early spring. RSV infection was associated with bronchiolitis, and parainfluenza was associated with croup; otherwise the clinical manifestations were largely nonspecific. In general, children infected with influenza A, adenovirus and mixed viruses had higher temperatures. In view of the increasing concern about unexpected outbreaks of severe viral infections, a rapid multiplex PCR assay is a valuable tool to enhance the management of hospitalized patients, and for the surveillance for viral infections circulating in the community.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19031443</PMID><DateCompleted><Year>2008</Year><Month>12</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1096-9071</ISSN><JournalIssue CitedMedium="Internet"><Volume>81</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of medical virology</Title><ISOAbbreviation>J. Med. Virol.</ISOAbbreviation></Journal><ArticleTitle>Identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in Hong Kong by multiplex PCR assays.</ArticleTitle><Pagination><MedlinePgn>153-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/jmv.21364</ELocationID><Abstract><AbstractText>Acute respiratory tract infection is a leading cause of hospital admission of children. This study used a broad capture, rapid and sensitive method (multiplex PCR assay) to detect 20 different respiratory pathogens including influenza A subtypes H1, H3, and H5; influenza B; parainfluenza types 1, 2, 3, and 4; respiratory syncytial virus (RSV) groups A and B; adenoviruses; human rhinoviruses; enteroviruses; human metapneumoviruses; human coronaviruses OC43, 229E, and SARS-CoV; Chlamydophila pneumoniae; Legionella pneumophila; and Mycoplasma pneumoniae; from respiratory specimens of 475 children hospitalized over a 12-month period for acute respiratory tract infections. The overall positive rate (47%) was about twice higher than previous reports based on conventional methods. Influenza A, parainfluenza and RSV accounted for 51%, and non-cultivable viruses accounted for 30% of positive cases. Influenza A peaked at March and June. Influenza B was detected in January, February, and April. Parainfluenza was prevalent throughout the year except from April to June. Most RSV infections were found between February and September. Adenovirus had multiple peaks, whereas rhinovirus and coronavirus OC43 were detected mainly in winter and early spring. RSV infection was associated with bronchiolitis, and parainfluenza was associated with croup; otherwise the clinical manifestations were largely nonspecific. In general, children infected with influenza A, adenovirus and mixed viruses had higher temperatures. In view of the increasing concern about unexpected outbreaks of severe viral infections, a rapid multiplex PCR assay is a valuable tool to enhance the management of hospitalized patients, and for the surveillance for viral infections circulating in the community.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sung</LastName><ForeName>R Y T</ForeName><Initials>RY</Initials><AffiliationInfo><Affiliation>Department of Paediatrics, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Paul K S</ForeName><Initials>PK</Initials></Author><Author ValidYN="Y"><LastName>Tsen</LastName><ForeName>Tracy</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>A M</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Lam</LastName><ForeName>W Y</ForeName><Initials>WY</Initials></Author><Author ValidYN="Y"><LastName>Yeung</LastName><ForeName>Apple C M</ForeName><Initials>AC</Initials></Author><Author ValidYN="Y"><LastName>Nelson</LastName><ForeName>E A S</ForeName><Initials>EA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Med Virol</MedlineTA><NlmUniqueID>7705876</NlmUniqueID><ISSNLinking>0146-6615</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001424" MajorTopicYN="N">Bacterial Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005122" MajorTopicYN="N">Exudates and Transudates</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006723" MajorTopicYN="N" Type="Geographic">Hong Kong</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" 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