SrasV1, PubMed, Corpus, bibRecord, 001A06

Expression and functional characterisation of the putative SARS coronavirus non-structural proteins X1-X5.

Identifieur interne : 001A06 ( PubMed/Corpus ); précédent : 001A05; suivant : 001A07

Expression and functional characterisation of the putative SARS coronavirus non-structural proteins X1-X5.

Auteurs : Y Y Ho

Source :

Hong Kong medical journal = Xianggang yi xue za zhi [ 1024-2708 ] ; 2008.

RBID : pubmed:18708666

English descriptors

KwdEn :
- Analysis of Variance, Animals, Case-Control Studies, Cell Proliferation, Cell Survival (genetics), Cell Survival (physiology), Cells, Cultured, Chlorocebus aethiops, Gene Expression Regulation, Viral, Genome, Viral (genetics), Microscopy, Confocal, Polymerase Chain Reaction, Probability, SARS Virus (genetics), SARS Virus (physiology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (physiopathology), Vero Cells (physiology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism).
MESH :
- chemical , genetics : Viral Envelope Proteins, Viral Nonstructural Proteins.
- genetics : Cell Survival, Genome, Viral, SARS Virus, Severe Acute Respiratory Syndrome.
- chemical , metabolism : Viral Envelope Proteins, Viral Nonstructural Proteins.
- physiology : Cell Survival, SARS Virus, Vero Cells.
- physiopathology : Severe Acute Respiratory Syndrome.
- Analysis of Variance, Animals, Case-Control Studies, Cell Proliferation, Cells, Cultured, Chlorocebus aethiops, Gene Expression Regulation, Viral, Microscopy, Confocal, Polymerase Chain Reaction, Probability, Sensitivity and Specificity.

Abstract

1. We produced mammalian expression vectors encoding the SARS coronavirus (SARS-CoV) accessory proteins with or without the fluorescence protein tag and cell lines with stable expression of these proteins. 2. The cellular localisation and function of the SARS-CoV accessory proteins was determined. 3. SARS 6 and SARS 8b proteins are localised to the endoplasmic reticulum and nucleus/cytoplasm, respectively, and both proteins stimulate host cell DNA synthesis.

PubMed: 18708666

Links to Exploration step

pubmed:18708666

Le document en format XML

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<author><name sortKey="Ho, Y Y" sort="Ho, Y Y" uniqKey="Ho Y" first="Y Y" last="Ho">Y Y Ho</name>
<affiliation><nlm:affiliation>Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. yuanyuanho@cuhk.edu.hk</nlm:affiliation>
</affiliation>
</author>
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<author><name sortKey="Ho, Y Y" sort="Ho, Y Y" uniqKey="Ho Y" first="Y Y" last="Ho">Y Y Ho</name>
<affiliation><nlm:affiliation>Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. yuanyuanho@cuhk.edu.hk</nlm:affiliation>
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<series><title level="j">Hong Kong medical journal = Xianggang yi xue za zhi</title>
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<imprint><date when="2008" type="published">2008</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance</term>
<term>Animals</term>
<term>Case-Control Studies</term>
<term>Cell Proliferation</term>
<term>Cell Survival (genetics)</term>
<term>Cell Survival (physiology)</term>
<term>Cells, Cultured</term>
<term>Chlorocebus aethiops</term>
<term>Gene Expression Regulation, Viral</term>
<term>Genome, Viral (genetics)</term>
<term>Microscopy, Confocal</term>
<term>Polymerase Chain Reaction</term>
<term>Probability</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
<term>Sensitivity and Specificity</term>
<term>Severe Acute Respiratory Syndrome (genetics)</term>
<term>Severe Acute Respiratory Syndrome (physiopathology)</term>
<term>Vero Cells (physiology)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Nonstructural Proteins (genetics)</term>
<term>Viral Nonstructural Proteins (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Envelope Proteins</term>
<term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Survival</term>
<term>Genome, Viral</term>
<term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Envelope Proteins</term>
<term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Survival</term>
<term>SARS Virus</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term>
<term>Animals</term>
<term>Case-Control Studies</term>
<term>Cell Proliferation</term>
<term>Cells, Cultured</term>
<term>Chlorocebus aethiops</term>
<term>Gene Expression Regulation, Viral</term>
<term>Microscopy, Confocal</term>
<term>Polymerase Chain Reaction</term>
<term>Probability</term>
<term>Sensitivity and Specificity</term>
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<front><div type="abstract" xml:lang="en">1. We produced mammalian expression vectors encoding the SARS coronavirus (SARS-CoV) accessory proteins with or without the fluorescence protein tag and cell lines with stable expression of these proteins. 2. The cellular localisation and function of the SARS-CoV accessory proteins was determined. 3. SARS 6 and SARS 8b proteins are localised to the endoplasmic reticulum and nucleus/cytoplasm, respectively, and both proteins stimulate host cell DNA synthesis.</div>
</front>
</TEI>
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<DateCompleted><Year>2009</Year>
<Month>10</Month>
<Day>14</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1024-2708</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>14 Suppl 4</Volume>
<PubDate><Year>2008</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Hong Kong medical journal = Xianggang yi xue za zhi</Title>
<ISOAbbreviation>Hong Kong Med J</ISOAbbreviation>
</Journal>
<ArticleTitle>Expression and functional characterisation of the putative SARS coronavirus non-structural proteins X1-X5.</ArticleTitle>
<Pagination><MedlinePgn>4-7</MedlinePgn>
</Pagination>
<Abstract><AbstractText>1. We produced mammalian expression vectors encoding the SARS coronavirus (SARS-CoV) accessory proteins with or without the fluorescence protein tag and cell lines with stable expression of these proteins. 2. The cellular localisation and function of the SARS-CoV accessory proteins was determined. 3. SARS 6 and SARS 8b proteins are localised to the endoplasmic reticulum and nucleus/cytoplasm, respectively, and both proteins stimulate host cell DNA synthesis.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ho</LastName>
<ForeName>Y Y</ForeName>
<Initials>YY</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. yuanyuanho@cuhk.edu.hk</Affiliation>
</AffiliationInfo>
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<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Hong Kong Med J</MedlineTA>
<NlmUniqueID>9512509</NlmUniqueID>
<ISSNLinking>1024-2708</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C087633">nonstructural protein, coronavirus</NameOfSubstance>
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<MeshHeading><DescriptorName UI="D016022" MajorTopicYN="N">Case-Control Studies</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
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<MeshHeading><DescriptorName UI="D015967" MajorTopicYN="Y">Gene Expression Regulation, Viral</DescriptorName>
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<MeshHeading><DescriptorName UI="D016679" MajorTopicYN="N">Genome, Viral</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011336" MajorTopicYN="N">Probability</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000503" MajorTopicYN="N">physiopathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year>
<Month>12</Month>
<Day>17</Day>
<Hour>9</Hour>
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<PubMedPubDate PubStatus="medline"><Year>2009</Year>
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Expression and functional characterisation of the putative SARS coronavirus non-structural proteins X1-X5.

Expression and functional characterisation of the putative SARS coronavirus non-structural proteins X1-X5.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki