Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage.
Identifieur interne : 001796 ( PubMed/Corpus ); précédent : 001795; suivant : 001797Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage.
Auteurs : Gergely Tekes ; Regina Hofmann-Lehmann ; Barbara Bank-Wolf ; Reinhard Maier ; Heinz-Jürgen Thiel ; Volker ThielSource :
- Journal of virology [ 1098-5514 ] ; 2010.
English descriptors
- KwdEn :
- Animals, Cats, Cell Line, Chimerism, Coronavirus, Feline (genetics), Coronavirus, Feline (growth & development), Coronavirus, Feline (physiology), Cricetinae, Flow Cytometry, Genes, Viral, Membrane Glycoproteins (genetics), Receptors, Virus (physiology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Coronavirus, Feline.
- growth & development : Coronavirus, Feline.
- physiology : Coronavirus, Feline, Receptors, Virus.
- Animals, Cats, Cell Line, Chimerism, Cricetinae, Flow Cytometry, Genes, Viral, Spike Glycoprotein, Coronavirus.
Abstract
Persistent infection of domestic cats with feline coronaviruses (FCoVs) can lead to a highly lethal, immunopathological disease termed feline infectious peritonitis (FIP). Interestingly, there are two serotypes, type I and type II FCoVs, that can cause both persistent infection and FIP, even though their main determinant of host cell tropism, the spike (S) protein, is of different phylogeny and displays limited sequence identity. In cell culture, however, there are apparent differences. Type II FCoVs can be propagated to high titers by employing feline aminopeptidase N (fAPN) as a cellular receptor, whereas the propagation of type I FCoVs is usually difficult, and the involvement of fAPN as a receptor is controversial. In this study we have analyzed the phenotypes of recombinant FCoVs that are based on the genetic background of type I FCoV strain Black but encode the type II FCoV strain 79-1146 S protein. Our data demonstrate that recombinant FCoVs expressing a type II FCoV S protein acquire the ability to efficiently use fAPN for host cell entry and corroborate the notion that type I FCoVs use another main host cell receptor. We also observed that recombinant FCoVs display a large-plaque phenotype and, unexpectedly, accelerated growth kinetics indistinguishable from that of type II FCoV strain 79-1146. Thus, the main phenotypic differences for type I and type II FCoVs in cell culture, namely, the growth kinetics and the efficient usage of fAPN as a cellular receptor, can be attributed solely to the FCoV S protein.
DOI: 10.1128/JVI.01568-09
PubMed: 19906918
Links to Exploration step
pubmed:19906918Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage.</title><author><name sortKey="Tekes, Gergely" sort="Tekes, Gergely" uniqKey="Tekes G" first="Gergely" last="Tekes">Gergely Tekes</name><affiliation><nlm:affiliation>Institut für Virologie, Justus Liebig Universität Giessen, Giessen, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Hofmann Lehmann, Regina" sort="Hofmann Lehmann, Regina" uniqKey="Hofmann Lehmann R" first="Regina" last="Hofmann-Lehmann">Regina Hofmann-Lehmann</name></author><author><name sortKey="Bank Wolf, Barbara" sort="Bank Wolf, Barbara" uniqKey="Bank Wolf B" first="Barbara" last="Bank-Wolf">Barbara Bank-Wolf</name></author><author><name sortKey="Maier, Reinhard" sort="Maier, Reinhard" uniqKey="Maier R" first="Reinhard" last="Maier">Reinhard Maier</name></author><author><name sortKey="Thiel, Heinz Jurgen" sort="Thiel, Heinz Jurgen" uniqKey="Thiel H" first="Heinz-Jürgen" last="Thiel">Heinz-Jürgen Thiel</name></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19906918</idno><idno type="pmid">19906918</idno><idno type="doi">10.1128/JVI.01568-09</idno><idno type="wicri:Area/PubMed/Corpus">001796</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001796</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage.</title><author><name sortKey="Tekes, Gergely" sort="Tekes, Gergely" uniqKey="Tekes G" first="Gergely" last="Tekes">Gergely Tekes</name><affiliation><nlm:affiliation>Institut für Virologie, Justus Liebig Universität Giessen, Giessen, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Hofmann Lehmann, Regina" sort="Hofmann Lehmann, Regina" uniqKey="Hofmann Lehmann R" first="Regina" last="Hofmann-Lehmann">Regina Hofmann-Lehmann</name></author><author><name sortKey="Bank Wolf, Barbara" sort="Bank Wolf, Barbara" uniqKey="Bank Wolf B" first="Barbara" last="Bank-Wolf">Barbara Bank-Wolf</name></author><author><name sortKey="Maier, Reinhard" sort="Maier, Reinhard" uniqKey="Maier R" first="Reinhard" last="Maier">Reinhard Maier</name></author><author><name sortKey="Thiel, Heinz Jurgen" sort="Thiel, Heinz Jurgen" uniqKey="Thiel H" first="Heinz-Jürgen" last="Thiel">Heinz-Jürgen Thiel</name></author><author><name sortKey="Thiel, Volker" sort="Thiel, Volker" uniqKey="Thiel V" first="Volker" last="Thiel">Volker Thiel</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cats</term><term>Cell Line</term><term>Chimerism</term><term>Coronavirus, Feline (genetics)</term><term>Coronavirus, Feline (growth & development)</term><term>Coronavirus, Feline (physiology)</term><term>Cricetinae</term><term>Flow Cytometry</term><term>Genes, Viral</term><term>Membrane Glycoproteins (genetics)</term><term>Receptors, Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus, Feline</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Coronavirus, Feline</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus, Feline</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Cell Line</term><term>Chimerism</term><term>Cricetinae</term><term>Flow Cytometry</term><term>Genes, Viral</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Persistent infection of domestic cats with feline coronaviruses (FCoVs) can lead to a highly lethal, immunopathological disease termed feline infectious peritonitis (FIP). Interestingly, there are two serotypes, type I and type II FCoVs, that can cause both persistent infection and FIP, even though their main determinant of host cell tropism, the spike (S) protein, is of different phylogeny and displays limited sequence identity. In cell culture, however, there are apparent differences. Type II FCoVs can be propagated to high titers by employing feline aminopeptidase N (fAPN) as a cellular receptor, whereas the propagation of type I FCoVs is usually difficult, and the involvement of fAPN as a receptor is controversial. In this study we have analyzed the phenotypes of recombinant FCoVs that are based on the genetic background of type I FCoV strain Black but encode the type II FCoV strain 79-1146 S protein. Our data demonstrate that recombinant FCoVs expressing a type II FCoV S protein acquire the ability to efficiently use fAPN for host cell entry and corroborate the notion that type I FCoVs use another main host cell receptor. We also observed that recombinant FCoVs display a large-plaque phenotype and, unexpectedly, accelerated growth kinetics indistinguishable from that of type II FCoV strain 79-1146. Thus, the main phenotypic differences for type I and type II FCoVs in cell culture, namely, the growth kinetics and the efficient usage of fAPN as a cellular receptor, can be attributed solely to the FCoV S protein.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19906918</PMID><DateCompleted><Year>2010</Year><Month>02</Month><Day>18</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5514</ISSN><JournalIssue CitedMedium="Internet"><Volume>84</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage.</ArticleTitle><Pagination><MedlinePgn>1326-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JVI.01568-09</ELocationID><Abstract><AbstractText>Persistent infection of domestic cats with feline coronaviruses (FCoVs) can lead to a highly lethal, immunopathological disease termed feline infectious peritonitis (FIP). Interestingly, there are two serotypes, type I and type II FCoVs, that can cause both persistent infection and FIP, even though their main determinant of host cell tropism, the spike (S) protein, is of different phylogeny and displays limited sequence identity. In cell culture, however, there are apparent differences. Type II FCoVs can be propagated to high titers by employing feline aminopeptidase N (fAPN) as a cellular receptor, whereas the propagation of type I FCoVs is usually difficult, and the involvement of fAPN as a receptor is controversial. In this study we have analyzed the phenotypes of recombinant FCoVs that are based on the genetic background of type I FCoV strain Black but encode the type II FCoV strain 79-1146 S protein. Our data demonstrate that recombinant FCoVs expressing a type II FCoV S protein acquire the ability to efficiently use fAPN for host cell entry and corroborate the notion that type I FCoVs use another main host cell receptor. We also observed that recombinant FCoVs display a large-plaque phenotype and, unexpectedly, accelerated growth kinetics indistinguishable from that of type II FCoV strain 79-1146. Thus, the main phenotypic differences for type I and type II FCoVs in cell culture, namely, the growth kinetics and the efficient usage of fAPN as a cellular receptor, can be attributed solely to the FCoV S protein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tekes</LastName><ForeName>Gergely</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Institut für Virologie, Justus Liebig Universität Giessen, Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hofmann-Lehmann</LastName><ForeName>Regina</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Bank-Wolf</LastName><ForeName>Barbara</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Maier</LastName><ForeName>Reinhard</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Thiel</LastName><ForeName>Heinz-Jürgen</ForeName><Initials>HJ</Initials></Author><Author 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Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002415" MajorTopicYN="N">Cats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046528" MajorTopicYN="Y">Chimerism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016765" MajorTopicYN="N">Coronavirus, Feline</DescriptorName><QualifierName 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