Establishment, immortalisation and characterisation of pteropid bat cell lines.
Identifieur interne : 001781 ( PubMed/Corpus ); précédent : 001780; suivant : 001782Establishment, immortalisation and characterisation of pteropid bat cell lines.
Auteurs : Gary Crameri ; Shawn Todd ; Samantha Grimley ; Jennifer A. Mceachern ; Glenn A. Marsh ; Craig Smith ; Mary Tachedjian ; Carol De Jong ; Elena R. Virtue ; Meng Yu ; Dieter Bulach ; Jun-Ping Liu ; Wojtek P. Michalski ; Deborah Middleton ; Hume E. Field ; Lin-Fa WangSource :
- PloS one [ 1932-6203 ] ; 2009.
English descriptors
- KwdEn :
- Animals, Cell Culture Techniques (methods), Cell Line, Transformed (cytology), Cell Shape (drug effects), Chiroptera, Cloning, Molecular, Hendra Virus (drug effects), Hendra Virus (physiology), Henipavirus Infections (virology), Humans, Immunity, Innate (drug effects), Immunity, Innate (immunology), Interferons (genetics), Nipah Virus (drug effects), Nipah Virus (physiology), Poly I-C (pharmacology), Simian virus 40 (genetics).
- MESH :
- chemical , genetics : Interferons.
- cytology : Cell Line, Transformed.
- drug effects : Cell Shape, Hendra Virus, Immunity, Innate, Nipah Virus.
- genetics : Simian virus 40.
- immunology : Immunity, Innate.
- methods : Cell Culture Techniques.
- chemical , pharmacology : Poly I-C.
- physiology : Hendra Virus, Nipah Virus.
- virology : Henipavirus Infections.
- Animals, Chiroptera, Cloning, Molecular, Humans.
Abstract
Bats are the suspected natural reservoir hosts for a number of new and emerging zoonotic viruses including Nipah virus, Hendra virus, severe acute respiratory syndrome coronavirus and Ebola virus. Since the discovery of SARS-like coronaviruses in Chinese horseshoe bats, attempts to isolate a SL-CoV from bats have failed and attempts to isolate other bat-borne viruses in various mammalian cell lines have been similarly unsuccessful. New stable bat cell lines are needed to help with these investigations and as tools to assist in the study of bat immunology and virus-host interactions.
DOI: 10.1371/journal.pone.0008266
PubMed: 20011515
Links to Exploration step
pubmed:20011515Le document en format XML
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Virtue</name></author><author><name sortKey="Yu, Meng" sort="Yu, Meng" uniqKey="Yu M" first="Meng" last="Yu">Meng Yu</name></author><author><name sortKey="Bulach, Dieter" sort="Bulach, Dieter" uniqKey="Bulach D" first="Dieter" last="Bulach">Dieter Bulach</name></author><author><name sortKey="Liu, Jun Ping" sort="Liu, Jun Ping" uniqKey="Liu J" first="Jun-Ping" last="Liu">Jun-Ping Liu</name></author><author><name sortKey="Michalski, Wojtek P" sort="Michalski, Wojtek P" uniqKey="Michalski W" first="Wojtek P" last="Michalski">Wojtek P. Michalski</name></author><author><name sortKey="Middleton, Deborah" sort="Middleton, Deborah" uniqKey="Middleton D" first="Deborah" last="Middleton">Deborah Middleton</name></author><author><name sortKey="Field, Hume E" sort="Field, Hume E" uniqKey="Field H" first="Hume E" last="Field">Hume E. 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Mceachern</name></author><author><name sortKey="Marsh, Glenn A" sort="Marsh, Glenn A" uniqKey="Marsh G" first="Glenn A" last="Marsh">Glenn A. Marsh</name></author><author><name sortKey="Smith, Craig" sort="Smith, Craig" uniqKey="Smith C" first="Craig" last="Smith">Craig Smith</name></author><author><name sortKey="Tachedjian, Mary" sort="Tachedjian, Mary" uniqKey="Tachedjian M" first="Mary" last="Tachedjian">Mary Tachedjian</name></author><author><name sortKey="De Jong, Carol" sort="De Jong, Carol" uniqKey="De Jong C" first="Carol" last="De Jong">Carol De Jong</name></author><author><name sortKey="Virtue, Elena R" sort="Virtue, Elena R" uniqKey="Virtue E" first="Elena R" last="Virtue">Elena R. Virtue</name></author><author><name sortKey="Yu, Meng" sort="Yu, Meng" uniqKey="Yu M" first="Meng" last="Yu">Meng Yu</name></author><author><name sortKey="Bulach, Dieter" sort="Bulach, Dieter" uniqKey="Bulach D" first="Dieter" last="Bulach">Dieter Bulach</name></author><author><name sortKey="Liu, Jun Ping" sort="Liu, Jun Ping" uniqKey="Liu J" first="Jun-Ping" last="Liu">Jun-Ping Liu</name></author><author><name sortKey="Michalski, Wojtek P" sort="Michalski, Wojtek P" uniqKey="Michalski W" first="Wojtek P" last="Michalski">Wojtek P. Michalski</name></author><author><name sortKey="Middleton, Deborah" sort="Middleton, Deborah" uniqKey="Middleton D" first="Deborah" last="Middleton">Deborah Middleton</name></author><author><name sortKey="Field, Hume E" sort="Field, Hume E" uniqKey="Field H" first="Hume E" last="Field">Hume E. Field</name></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Culture Techniques (methods)</term><term>Cell Line, Transformed (cytology)</term><term>Cell Shape (drug effects)</term><term>Chiroptera</term><term>Cloning, Molecular</term><term>Hendra Virus (drug effects)</term><term>Hendra Virus (physiology)</term><term>Henipavirus Infections (virology)</term><term>Humans</term><term>Immunity, Innate (drug effects)</term><term>Immunity, Innate (immunology)</term><term>Interferons (genetics)</term><term>Nipah Virus (drug effects)</term><term>Nipah Virus (physiology)</term><term>Poly I-C (pharmacology)</term><term>Simian virus 40 (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Interferons</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Cell Line, Transformed</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Shape</term><term>Hendra Virus</term><term>Immunity, Innate</term><term>Nipah Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Simian virus 40</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Immunity, Innate</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cell Culture Techniques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Poly I-C</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Hendra Virus</term><term>Nipah Virus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Henipavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chiroptera</term><term>Cloning, Molecular</term><term>Humans</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bats are the suspected natural reservoir hosts for a number of new and emerging zoonotic viruses including Nipah virus, Hendra virus, severe acute respiratory syndrome coronavirus and Ebola virus. Since the discovery of SARS-like coronaviruses in Chinese horseshoe bats, attempts to isolate a SL-CoV from bats have failed and attempts to isolate other bat-borne viruses in various mammalian cell lines have been similarly unsuccessful. New stable bat cell lines are needed to help with these investigations and as tools to assist in the study of bat immunology and virus-host interactions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20011515</PMID><DateCompleted><Year>2010</Year><Month>03</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>4</Volume><Issue>12</Issue><PubDate><Year>2009</Year><Month>Dec</Month><Day>11</Day></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS ONE</ISOAbbreviation></Journal><ArticleTitle>Establishment, immortalisation and characterisation of pteropid bat cell lines.</ArticleTitle><Pagination><MedlinePgn>e8266</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0008266</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Bats are the suspected natural reservoir hosts for a number of new and emerging zoonotic viruses including Nipah virus, Hendra virus, severe acute respiratory syndrome coronavirus and Ebola virus. Since the discovery of SARS-like coronaviruses in Chinese horseshoe bats, attempts to isolate a SL-CoV from bats have failed and attempts to isolate other bat-borne viruses in various mammalian cell lines have been similarly unsuccessful. New stable bat cell lines are needed to help with these investigations and as tools to assist in the study of bat immunology and virus-host interactions.</AbstractText><AbstractText Label="METHODOLOGY/FINDINGS" NlmCategory="RESULTS">Black flying foxes (Pteropus alecto) were captured from the wild and transported live to the laboratory for primary cell culture preparation using a variety of different methods and culture media. Primary cells were successfully cultured from 20 different organs. Cell immortalisation can occur spontaneously, however we used a retroviral system to immortalise cells via the transfer and stable production of the Simian virus 40 Large T antigen and the human telomerase reverse transcriptase protein. Initial infection experiments with both cloned and uncloned cell lines using Hendra and Nipah viruses demonstrated varying degrees of infection efficiency between the different cell lines, although it was possible to infect cells in all tissue types.</AbstractText><AbstractText Label="CONCLUSIONS/SIGNIFICANCE" NlmCategory="CONCLUSIONS">The approaches developed and optimised in this study should be applicable to bats of other species. We are in the process of generating further cell lines from a number of different bat species using the methodology established in this study.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Crameri</LastName><ForeName>Gary</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Todd</LastName><ForeName>Shawn</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Grimley</LastName><ForeName>Samantha</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>McEachern</LastName><ForeName>Jennifer A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Marsh</LastName><ForeName>Glenn A</ForeName><Initials>GA</Initials></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>Craig</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Tachedjian</LastName><ForeName>Mary</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>De Jong</LastName><ForeName>Carol</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Virtue</LastName><ForeName>Elena R</ForeName><Initials>ER</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Meng</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Bulach</LastName><ForeName>Dieter</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jun-Ping</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Michalski</LastName><ForeName>Wojtek P</ForeName><Initials>WP</Initials></Author><Author ValidYN="Y"><LastName>Middleton</LastName><ForeName>Deborah</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Field</LastName><ForeName>Hume E</ForeName><Initials>HE</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lin-Fa</ForeName><Initials>LF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>12</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9008-11-1</RegistryNumber><NameOfSubstance UI="D007372">Interferons</NameOfSubstance></Chemical><Chemical><RegistryNumber>O84C90HH2L</RegistryNumber><NameOfSubstance UI="D011070">Poly I-C</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018929" MajorTopicYN="N">Cell Culture Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002461" MajorTopicYN="N">Cell Line, Transformed</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D048430" MajorTopicYN="N">Cell Shape</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002685" MajorTopicYN="Y">Chiroptera</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045404" MajorTopicYN="N">Hendra Virus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045464" MajorTopicYN="N">Henipavirus Infections</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007372" MajorTopicYN="N">Interferons</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045405" MajorTopicYN="N">Nipah Virus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011070" MajorTopicYN="N">Poly I-C</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013539" MajorTopicYN="N">Simian virus 40</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>11</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>11</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>12</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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