Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection.
Identifieur interne : 001764 ( PubMed/Corpus ); précédent : 001763; suivant : 001765Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection.
Auteurs : Tomoki Yoshikawa ; Terence E. Hill ; Naoko Yoshikawa ; Vsevolod L. Popov ; Cristi L. Galindo ; Harold R. Garner ; C J Peters ; Chien-Te Kent TsengSource :
- PloS one [ 1932-6203 ] ; 2010.
English descriptors
- KwdEn :
- Bronchi (cytology), Bronchi (immunology), Cells, Cultured, Epithelial Cells (immunology), Gene Expression, Humans, Immunity, Innate, Interferon Regulatory Factor-3 (metabolism), Interferon Regulatory Factor-7 (metabolism), NF-kappa B (metabolism), SARS Virus (genetics), SARS Virus (physiology), Severe Acute Respiratory Syndrome (immunology), Virus Replication.
- MESH :
- chemical , metabolism : Interferon Regulatory Factor-3, Interferon Regulatory Factor-7, NF-kappa B.
- cytology : Bronchi.
- genetics : SARS Virus.
- immunology : Bronchi, Epithelial Cells, Severe Acute Respiratory Syndrome.
- physiology : SARS Virus.
- Cells, Cultured, Gene Expression, Humans, Immunity, Innate, Virus Replication.
Abstract
Human lung epithelial cells are likely among the first targets to encounter invading severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Not only can these cells support the growth of SARS-CoV infection, but they are also capable of secreting inflammatory cytokines to initiate and, eventually, aggravate host innate inflammatory responses, causing detrimental immune-mediated pathology within the lungs. Thus, a comprehensive evaluation of the complex epithelial signaling to SARS-CoV is crucial for paving the way to better understand SARS pathogenesis. Based on microarray-based functional genomics, we report here the global gene response of 2B4 cells, a cloned bronchial epithelial cell line derived from Calu-3 cells. Specifically, we found a temporal and spatial activation of nuclear factor (NF)kappaB, activator protein (AP)-1, and interferon regulatory factor (IRF)-3/7 in infected 2B4 cells at 12-, 24-, and 48-hrs post infection (p.i.), resulting in the activation of many antiviral genes, including interferon (IFN)-beta, -lambdas, inflammatory mediators, and many IFN-stimulated genes (ISGs). We also showed, for the first time, that IFN-beta and IFN-lambdas were capable of exerting previously unrecognized, non-redundant, and complementary abilities to limit SARS-CoV replication, even though their expression could not be detected in infected 2B4 bronchial epithelial cells until 48 hrs p.i. Collectively, our results highlight the mechanics of the sequential events of antiviral signaling pathway/s triggered by SARS-CoV in bronchial epithelial cells and identify novel cellular targets for future studies, aiming at advancing strategies against SARS.
DOI: 10.1371/journal.pone.0008729
PubMed: 20090954
Links to Exploration step
pubmed:20090954Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection.</title><author><name sortKey="Yoshikawa, Tomoki" sort="Yoshikawa, Tomoki" uniqKey="Yoshikawa T" first="Tomoki" last="Yoshikawa">Tomoki Yoshikawa</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, United States of America.</nlm:affiliation></affiliation></author><author><name sortKey="Hill, Terence E" sort="Hill, Terence E" uniqKey="Hill T" first="Terence E" last="Hill">Terence E. Hill</name></author><author><name sortKey="Yoshikawa, Naoko" sort="Yoshikawa, Naoko" uniqKey="Yoshikawa N" first="Naoko" last="Yoshikawa">Naoko Yoshikawa</name></author><author><name sortKey="Popov, Vsevolod L" sort="Popov, Vsevolod L" uniqKey="Popov V" first="Vsevolod L" last="Popov">Vsevolod L. Popov</name></author><author><name sortKey="Galindo, Cristi L" sort="Galindo, Cristi L" uniqKey="Galindo C" first="Cristi L" last="Galindo">Cristi L. Galindo</name></author><author><name sortKey="Garner, Harold R" sort="Garner, Harold R" uniqKey="Garner H" first="Harold R" last="Garner">Harold R. Garner</name></author><author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name></author><author><name sortKey="Tseng, Chien Te Kent" sort="Tseng, Chien Te Kent" uniqKey="Tseng C" first="Chien-Te Kent" last="Tseng">Chien-Te Kent Tseng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20090954</idno><idno type="pmid">20090954</idno><idno type="doi">10.1371/journal.pone.0008729</idno><idno type="wicri:Area/PubMed/Corpus">001764</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001764</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection.</title><author><name sortKey="Yoshikawa, Tomoki" sort="Yoshikawa, Tomoki" uniqKey="Yoshikawa T" first="Tomoki" last="Yoshikawa">Tomoki Yoshikawa</name><affiliation><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, United States of America.</nlm:affiliation></affiliation></author><author><name sortKey="Hill, Terence E" sort="Hill, Terence E" uniqKey="Hill T" first="Terence E" last="Hill">Terence E. Hill</name></author><author><name sortKey="Yoshikawa, Naoko" sort="Yoshikawa, Naoko" uniqKey="Yoshikawa N" first="Naoko" last="Yoshikawa">Naoko Yoshikawa</name></author><author><name sortKey="Popov, Vsevolod L" sort="Popov, Vsevolod L" uniqKey="Popov V" first="Vsevolod L" last="Popov">Vsevolod L. Popov</name></author><author><name sortKey="Galindo, Cristi L" sort="Galindo, Cristi L" uniqKey="Galindo C" first="Cristi L" last="Galindo">Cristi L. Galindo</name></author><author><name sortKey="Garner, Harold R" sort="Garner, Harold R" uniqKey="Garner H" first="Harold R" last="Garner">Harold R. Garner</name></author><author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name></author><author><name sortKey="Tseng, Chien Te Kent" sort="Tseng, Chien Te Kent" uniqKey="Tseng C" first="Chien-Te Kent" last="Tseng">Chien-Te Kent Tseng</name></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bronchi (cytology)</term><term>Bronchi (immunology)</term><term>Cells, Cultured</term><term>Epithelial Cells (immunology)</term><term>Gene Expression</term><term>Humans</term><term>Immunity, Innate</term><term>Interferon Regulatory Factor-3 (metabolism)</term><term>Interferon Regulatory Factor-7 (metabolism)</term><term>NF-kappa B (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Virus Replication</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon Regulatory Factor-3</term><term>Interferon Regulatory Factor-7</term><term>NF-kappa B</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Bronchi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Bronchi</term><term>Epithelial Cells</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Gene Expression</term><term>Humans</term><term>Immunity, Innate</term><term>Virus Replication</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human lung epithelial cells are likely among the first targets to encounter invading severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Not only can these cells support the growth of SARS-CoV infection, but they are also capable of secreting inflammatory cytokines to initiate and, eventually, aggravate host innate inflammatory responses, causing detrimental immune-mediated pathology within the lungs. Thus, a comprehensive evaluation of the complex epithelial signaling to SARS-CoV is crucial for paving the way to better understand SARS pathogenesis. Based on microarray-based functional genomics, we report here the global gene response of 2B4 cells, a cloned bronchial epithelial cell line derived from Calu-3 cells. Specifically, we found a temporal and spatial activation of nuclear factor (NF)kappaB, activator protein (AP)-1, and interferon regulatory factor (IRF)-3/7 in infected 2B4 cells at 12-, 24-, and 48-hrs post infection (p.i.), resulting in the activation of many antiviral genes, including interferon (IFN)-beta, -lambdas, inflammatory mediators, and many IFN-stimulated genes (ISGs). We also showed, for the first time, that IFN-beta and IFN-lambdas were capable of exerting previously unrecognized, non-redundant, and complementary abilities to limit SARS-CoV replication, even though their expression could not be detected in infected 2B4 bronchial epithelial cells until 48 hrs p.i. Collectively, our results highlight the mechanics of the sequential events of antiviral signaling pathway/s triggered by SARS-CoV in bronchial epithelial cells and identify novel cellular targets for future studies, aiming at advancing strategies against SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20090954</PMID><DateCompleted><Year>2010</Year><Month>05</Month><Day>20</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Jan</Month><Day>15</Day></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS ONE</ISOAbbreviation></Journal><ArticleTitle>Dynamic innate immune responses of human bronchial epithelial cells to severe acute respiratory syndrome-associated coronavirus infection.</ArticleTitle><Pagination><MedlinePgn>e8729</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0008729</ELocationID><Abstract><AbstractText>Human lung epithelial cells are likely among the first targets to encounter invading severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Not only can these cells support the growth of SARS-CoV infection, but they are also capable of secreting inflammatory cytokines to initiate and, eventually, aggravate host innate inflammatory responses, causing detrimental immune-mediated pathology within the lungs. Thus, a comprehensive evaluation of the complex epithelial signaling to SARS-CoV is crucial for paving the way to better understand SARS pathogenesis. Based on microarray-based functional genomics, we report here the global gene response of 2B4 cells, a cloned bronchial epithelial cell line derived from Calu-3 cells. Specifically, we found a temporal and spatial activation of nuclear factor (NF)kappaB, activator protein (AP)-1, and interferon regulatory factor (IRF)-3/7 in infected 2B4 cells at 12-, 24-, and 48-hrs post infection (p.i.), resulting in the activation of many antiviral genes, including interferon (IFN)-beta, -lambdas, inflammatory mediators, and many IFN-stimulated genes (ISGs). We also showed, for the first time, that IFN-beta and IFN-lambdas were capable of exerting previously unrecognized, non-redundant, and complementary abilities to limit SARS-CoV replication, even though their expression could not be detected in infected 2B4 bronchial epithelial cells until 48 hrs p.i. Collectively, our results highlight the mechanics of the sequential events of antiviral signaling pathway/s triggered by SARS-CoV in bronchial epithelial cells and identify novel cellular targets for future studies, aiming at advancing strategies against SARS.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yoshikawa</LastName><ForeName>Tomoki</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hill</LastName><ForeName>Terence E</ForeName><Initials>TE</Initials></Author><Author ValidYN="Y"><LastName>Yoshikawa</LastName><ForeName>Naoko</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Popov</LastName><ForeName>Vsevolod L</ForeName><Initials>VL</Initials></Author><Author ValidYN="Y"><LastName>Galindo</LastName><ForeName>Cristi L</ForeName><Initials>CL</Initials></Author><Author ValidYN="Y"><LastName>Garner</LastName><ForeName>Harold R</ForeName><Initials>HR</Initials></Author><Author ValidYN="Y"><LastName>Peters</LastName><ForeName>C J</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Tseng</LastName><ForeName>Chien-Te Kent</ForeName><Initials>CT</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>U54 AI057156</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>N01AI25489</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21AI072201</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R21 AI072201</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>N01 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IdType="pubmed">6173610</ArticleId></ArticleIdList></Reference><Reference><Citation>Can J Comp Med. 1983 Jan;47(1):64-9</Citation><ArticleIdList><ArticleId IdType="pubmed">6299486</ArticleId></ArticleIdList></Reference><Reference><Citation>Am Rev Respir Dis. 1990 Mar;141(3 Pt 2):S141-4</Citation><ArticleIdList><ArticleId IdType="pubmed">2155562</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 1993 Jun 1;177(6):1809-14</Citation><ArticleIdList><ArticleId IdType="pubmed">8496693</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Respir Cell Mol Biol. 1993 Nov;9(5):547-56</Citation><ArticleIdList><ArticleId IdType="pubmed">7692897</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Physiol. 1994 May;266(5 Pt 1):L493-501</Citation><ArticleIdList><ArticleId IdType="pubmed">7515578</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 1995 Oct 15;155(8):3877-88</Citation><ArticleIdList><ArticleId IdType="pubmed">7561094</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1995 Dec 29;83(7):1091-100</Citation><ArticleIdList><ArticleId IdType="pubmed">8548797</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2008 Mar;4(3):e1000017</Citation><ArticleIdList><ArticleId IdType="pubmed">18369468</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Trop Med Hyg. 2008 Apr;78(4):675-80</Citation><ArticleIdList><ArticleId IdType="pubmed">18385368</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2008 May;82(9):4471-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18305050</ArticleId></ArticleIdList></Reference><Reference><Citation>Exp Physiol. 2008 May;93(5):543-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18448662</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W341-6</Citation><ArticleIdList><ArticleId IdType="pubmed">18515841</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2008;4(9):e1000151</Citation><ArticleIdList><ArticleId IdType="pubmed">18787692</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 Apr;83(7):3039-48</Citation><ArticleIdList><ArticleId IdType="pubmed">19004938</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 Jun;83(11):5451-65</Citation><ArticleIdList><ArticleId IdType="pubmed">19297479</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2009 Jun 12;284(24):16202-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19380580</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Chem. 2005 Dec;51(12):2333-40</Citation><ArticleIdList><ArticleId IdType="pubmed">16195357</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Dec;79(24):15511-24</Citation><ArticleIdList><ArticleId IdType="pubmed">16306622</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2006 Jan 5;344(1):119-30</Citation><ArticleIdList><ArticleId IdType="pubmed">16364743</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Jan;80(2):785-93</Citation><ArticleIdList><ArticleId IdType="pubmed">16378980</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2006 Feb;16(2):141-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16474426</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur Respir J. 1997 Sep;10(9):2139-46</Citation><ArticleIdList><ArticleId IdType="pubmed">9311517</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 1998 Mar;1(4):507-18</Citation><ArticleIdList><ArticleId IdType="pubmed">9660935</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1998 Nov 16;17(22):6660-9</Citation><ArticleIdList><ArticleId IdType="pubmed">9822609</ArticleId></ArticleIdList></Reference><Reference><Citation>J Allergy Clin Immunol. 1998 Nov;102(5):714-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9867498</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1998 Dec 11;441(1):106-10</Citation><ArticleIdList><ArticleId IdType="pubmed">9877175</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2005 Jan 28;326(4):905-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15607755</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Virol. 2005 Feb;75(2):185-94</Citation><ArticleIdList><ArticleId IdType="pubmed">15602737</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Immunol. 2005;6:2</Citation><ArticleIdList><ArticleId IdType="pubmed">15655079</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Feb;79(4):2079-86</Citation><ArticleIdList><ArticleId IdType="pubmed">15681410</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2005 Apr 7;434(7034):772-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15800576</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Respir Crit Care Med. 2005 Apr 15;171(8):850-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15657466</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbes Infect. 2005 Feb;7(2):248-59</Citation><ArticleIdList><ArticleId IdType="pubmed">15777647</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 May;79(10):6180-93</Citation><ArticleIdList><ArticleId IdType="pubmed">15858003</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Jun;79(12):7819-26</Citation><ArticleIdList><ArticleId IdType="pubmed">15919935</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Aug;79(15):9470-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16014910</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Dec;79(23):14614-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16282461</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12885-90</Citation><ArticleIdList><ArticleId IdType="pubmed">16912115</ArticleId></ArticleIdList></Reference><Reference><Citation>Antivir Chem Chemother. 2006;17(5):275-84</Citation><ArticleIdList><ArticleId IdType="pubmed">17176632</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Jan;81(2):568-74</Citation><ArticleIdList><ArticleId IdType="pubmed">17079305</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Jan;81(2):548-57</Citation><ArticleIdList><ArticleId IdType="pubmed">17108024</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Feb;81(3):1162-73</Citation><ArticleIdList><ArticleId IdType="pubmed">17108019</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2007 Mar 9;282(10):7576-81</Citation><ArticleIdList><ArticleId IdType="pubmed">17204473</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Trop Med Hyg. 2007 Apr;76(4):785-90</Citation><ArticleIdList><ArticleId IdType="pubmed">17426188</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2007 Apr 25;361(1):18-26</Citation><ArticleIdList><ArticleId IdType="pubmed">17316733</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Jul;81(13):7189-99</Citation><ArticleIdList><ArticleId IdType="pubmed">17459917</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2007 Jul;35(Web Server issue):W91-6</Citation><ArticleIdList><ArticleId IdType="pubmed">17478504</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Sep;81(18):9812-24</Citation><ArticleIdList><ArticleId IdType="pubmed">17596301</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2007 Aug 10;3(8):e112</Citation><ArticleIdList><ArticleId IdType="pubmed">17696609</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Nov;81(21):11620-33</Citation><ArticleIdList><ArticleId IdType="pubmed">17715225</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Med. 2006 Feb;3(2):e27</Citation><ArticleIdList><ArticleId IdType="pubmed">16379499</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2006;3:17</Citation><ArticleIdList><ArticleId IdType="pubmed">16571117</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2006 Aug 15;352(1):14-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16753195</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Immunol. 2000;18:621-63</Citation><ArticleIdList><ArticleId IdType="pubmed">10837071</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2000 Nov 10;103(4):667-78</Citation><ArticleIdList><ArticleId IdType="pubmed">11106736</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunity. 2000 Oct;13(4):539-48</Citation><ArticleIdList><ArticleId IdType="pubmed">11070172</ArticleId></ArticleIdList></Reference><Reference><Citation>Behav Brain Res. 2001 Nov 1;125(1-2):279-84</Citation><ArticleIdList><ArticleId IdType="pubmed">11682119</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2002 Apr 12;296(5566):298-300</Citation><ArticleIdList><ArticleId IdType="pubmed">11951031</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2002 Feb 20;285(1-2):1-24</Citation><ArticleIdList><ArticleId IdType="pubmed">12039028</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2003 Jan 22;19(2):185-93</Citation><ArticleIdList><ArticleId IdType="pubmed">12538238</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Surg Pathol. 2003 Apr;11(2):118</Citation><ArticleIdList><ArticleId IdType="pubmed">12754629</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 May 24;361(9371):1773-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12781536</ArticleId></ArticleIdList></Reference><Reference><Citation>BMJ. 2003 Jun 21;326(7403):1358-62</Citation><ArticleIdList><ArticleId IdType="pubmed">12816821</ArticleId></ArticleIdList></Reference><Reference><Citation>Chin Med J (Engl). 2003 Jul;116(7):976-80</Citation><ArticleIdList><ArticleId IdType="pubmed">12890365</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2004 Mar;10(3):290-3</Citation><ArticleIdList><ArticleId IdType="pubmed">14981511</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2004 Feb;10(2):317-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15030704</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pathol. 2004 Jun;203(2):729-30; author reply 730-1</Citation><ArticleIdList><ArticleId IdType="pubmed">15141389</ArticleId></ArticleIdList></Reference><Reference><Citation>Infect Immun. 2004 Aug;72(8):4410-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15271897</ArticleId></ArticleIdList></Reference><Reference><Citation>J Interferon Cytokine Res. 2004 Jul;24(7):388-90</Citation><ArticleIdList><ArticleId IdType="pubmed">15296649</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 2004 Aug;61(16):2100-12</Citation><ArticleIdList><ArticleId IdType="pubmed">15316659</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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