The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication.
Identifieur interne : 001681 ( PubMed/Corpus ); précédent : 001680; suivant : 001682The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication.
Auteurs : Linghui Xu ; Siti Khadijah ; Shouguo Fang ; Li Wang ; Felicia P L. Tay ; Ding Xiang LiuSource :
- Journal of virology [ 1098-5514 ] ; 2010.
English descriptors
- KwdEn :
- Animals, Cell Nucleus (metabolism), Chlorocebus aethiops, Coronavirus Infections (genetics), Coronavirus Infections (metabolism), Coronavirus Infections (virology), Cytoplasm (metabolism), DEAD-box RNA Helicases (genetics), DEAD-box RNA Helicases (metabolism), HeLa Cells, Humans, Infectious bronchitis virus (genetics), Infectious bronchitis virus (physiology), Protein Binding, Protein Transport, Two-Hybrid System Techniques, Vero Cells, Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Virus Replication.
- MESH :
- chemical , genetics : DEAD-box RNA Helicases, Viral Nonstructural Proteins.
- genetics : Coronavirus Infections, Infectious bronchitis virus.
- metabolism : Cell Nucleus, Coronavirus Infections, Cytoplasm, DEAD-box RNA Helicases, Viral Nonstructural Proteins.
- physiology : Infectious bronchitis virus.
- virology : Coronavirus Infections.
- Animals, Chlorocebus aethiops, HeLa Cells, Humans, Protein Binding, Protein Transport, Two-Hybrid System Techniques, Vero Cells, Virus Replication.
Abstract
The involvement of host proteins in the replication and transcription of viral RNA is a poorly understood area for many RNA viruses. For coronaviruses, it was long speculated that replication of the giant RNA genome and transcription of multiple subgenomic mRNA species by a unique discontinuous transcription mechanism may require host cofactors. To search for such cellular proteins, yeast two-hybrid screening was carried out by using the nonstructural protein 14 (nsp14) from the coronavirus infectious bronchitis virus (IBV) as a bait protein, leading to the identification of DDX1, a cellular RNA helicase in the DExD/H helicase family, as a potential interacting partner. This interaction was subsequently confirmed by coimmunoprecipitation assays with cells coexpressing the two proteins and with IBV-infected cells. Furthermore, the endogenous DDX1 protein was found to be relocated from the nucleus to the cytoplasm in IBV-infected cells. In addition to its interaction with IBV nsp14, DDX1 could also interact with the nsp14 protein from severe acute respiratory syndrome coronavirus (SARS-CoV), suggesting that interaction with DDX1 may be a general feature of coronavirus nsp14. The interacting domains were mapped to the C-terminal region of DDX1 containing motifs V and VI and to the N-terminal portion of nsp14. Manipulation of DDX1 expression, either by small interfering RNA-induced knockdown or by overexpression of a mutant DDX1 protein, confirmed that this interaction may enhance IBV replication. This study reveals that DDX1 contributes to efficient coronavirus replication in cell culture.
DOI: 10.1128/JVI.00392-10
PubMed: 20573827
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pubmed:20573827Le document en format XML
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Tay</name></author><author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20573827</idno><idno type="pmid">20573827</idno><idno type="doi">10.1128/JVI.00392-10</idno><idno type="wicri:Area/PubMed/Corpus">001681</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001681</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication.</title><author><name sortKey="Xu, Linghui" sort="Xu, Linghui" uniqKey="Xu L" first="Linghui" last="Xu">Linghui Xu</name><affiliation><nlm:affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore.</nlm:affiliation></affiliation></author><author><name sortKey="Khadijah, Siti" sort="Khadijah, Siti" uniqKey="Khadijah S" first="Siti" last="Khadijah">Siti Khadijah</name></author><author><name sortKey="Fang, Shouguo" sort="Fang, Shouguo" uniqKey="Fang S" first="Shouguo" last="Fang">Shouguo Fang</name></author><author><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name></author><author><name sortKey="Tay, Felicia P L" sort="Tay, Felicia P L" uniqKey="Tay F" first="Felicia P L" last="Tay">Felicia P L. Tay</name></author><author><name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Nucleus (metabolism)</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (genetics)</term><term>Coronavirus Infections (metabolism)</term><term>Coronavirus Infections (virology)</term><term>Cytoplasm (metabolism)</term><term>DEAD-box RNA Helicases (genetics)</term><term>DEAD-box RNA Helicases (metabolism)</term><term>HeLa Cells</term><term>Humans</term><term>Infectious bronchitis virus (genetics)</term><term>Infectious bronchitis virus (physiology)</term><term>Protein Binding</term><term>Protein Transport</term><term>Two-Hybrid System Techniques</term><term>Vero Cells</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Virus Replication</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DEAD-box RNA Helicases</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus Infections</term><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term><term>Coronavirus Infections</term><term>Cytoplasm</term><term>DEAD-box RNA Helicases</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>HeLa Cells</term><term>Humans</term><term>Protein Binding</term><term>Protein Transport</term><term>Two-Hybrid System Techniques</term><term>Vero Cells</term><term>Virus Replication</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The involvement of host proteins in the replication and transcription of viral RNA is a poorly understood area for many RNA viruses. For coronaviruses, it was long speculated that replication of the giant RNA genome and transcription of multiple subgenomic mRNA species by a unique discontinuous transcription mechanism may require host cofactors. To search for such cellular proteins, yeast two-hybrid screening was carried out by using the nonstructural protein 14 (nsp14) from the coronavirus infectious bronchitis virus (IBV) as a bait protein, leading to the identification of DDX1, a cellular RNA helicase in the DExD/H helicase family, as a potential interacting partner. This interaction was subsequently confirmed by coimmunoprecipitation assays with cells coexpressing the two proteins and with IBV-infected cells. Furthermore, the endogenous DDX1 protein was found to be relocated from the nucleus to the cytoplasm in IBV-infected cells. In addition to its interaction with IBV nsp14, DDX1 could also interact with the nsp14 protein from severe acute respiratory syndrome coronavirus (SARS-CoV), suggesting that interaction with DDX1 may be a general feature of coronavirus nsp14. The interacting domains were mapped to the C-terminal region of DDX1 containing motifs V and VI and to the N-terminal portion of nsp14. Manipulation of DDX1 expression, either by small interfering RNA-induced knockdown or by overexpression of a mutant DDX1 protein, confirmed that this interaction may enhance IBV replication. This study reveals that DDX1 contributes to efficient coronavirus replication in cell culture.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20573827</PMID><DateCompleted><Year>2010</Year><Month>09</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5514</ISSN><JournalIssue CitedMedium="Internet"><Volume>84</Volume><Issue>17</Issue><PubDate><Year>2010</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication.</ArticleTitle><Pagination><MedlinePgn>8571-83</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JVI.00392-10</ELocationID><Abstract><AbstractText>The involvement of host proteins in the replication and transcription of viral RNA is a poorly understood area for many RNA viruses. For coronaviruses, it was long speculated that replication of the giant RNA genome and transcription of multiple subgenomic mRNA species by a unique discontinuous transcription mechanism may require host cofactors. To search for such cellular proteins, yeast two-hybrid screening was carried out by using the nonstructural protein 14 (nsp14) from the coronavirus infectious bronchitis virus (IBV) as a bait protein, leading to the identification of DDX1, a cellular RNA helicase in the DExD/H helicase family, as a potential interacting partner. This interaction was subsequently confirmed by coimmunoprecipitation assays with cells coexpressing the two proteins and with IBV-infected cells. Furthermore, the endogenous DDX1 protein was found to be relocated from the nucleus to the cytoplasm in IBV-infected cells. In addition to its interaction with IBV nsp14, DDX1 could also interact with the nsp14 protein from severe acute respiratory syndrome coronavirus (SARS-CoV), suggesting that interaction with DDX1 may be a general feature of coronavirus nsp14. The interacting domains were mapped to the C-terminal region of DDX1 containing motifs V and VI and to the N-terminal portion of nsp14. Manipulation of DDX1 expression, either by small interfering RNA-induced knockdown or by overexpression of a mutant DDX1 protein, confirmed that this interaction may enhance IBV replication. This study reveals that DDX1 contributes to efficient coronavirus replication in cell culture.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Linghui</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khadijah</LastName><ForeName>Siti</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Shouguo</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Tay</LastName><ForeName>Felicia P L</ForeName><Initials>FP</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Ding Xiang</ForeName><Initials>DX</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType 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Helicases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018352" MajorTopicYN="N">Coronavirus Infections</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000378" 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PubStatus="medline"><Year>2010</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20573827</ArticleId><ArticleId IdType="pii">JVI.00392-10</ArticleId><ArticleId IdType="doi">10.1128/JVI.00392-10</ArticleId><ArticleId IdType="pmc">PMC2918985</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Mol Biol. 2005 Nov 11;353(5):1106-17</Citation><ArticleIdList><ArticleId IdType="pubmed">16216269</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2005 Oct 21;336(2):417-23</Citation><ArticleIdList><ArticleId IdType="pubmed">16137658</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5108-13</Citation><ArticleIdList><ArticleId IdType="pubmed">16549795</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2006 Jun;87(Pt 6):1403-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16690906</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2005 Dec;1(4):e39</Citation><ArticleIdList><ArticleId IdType="pubmed">16341254</ArticleId></ArticleIdList></Reference><Reference><Citation>Oncogene. 2006 Jul 20;25(31):4320-31</Citation><ArticleIdList><ArticleId IdType="pubmed">16518412</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2006 Sep 1;347(3):683-91</Citation><ArticleIdList><ArticleId IdType="pubmed">16842740</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2006 Aug 11;361(2):243-56</Citation><ArticleIdList><ArticleId IdType="pubmed">16828802</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11892-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16882730</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2006;34(15):4206-15</Citation><ArticleIdList><ArticleId IdType="pubmed">16935882</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Nov;80(21):10900-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16928748</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2006;34(17):4816-25</Citation><ArticleIdList><ArticleId IdType="pubmed">16971454</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Jan;81(1):20-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16928755</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010 May;6(5):e1000896</Citation><ArticleIdList><ArticleId IdType="pubmed">20463816</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2000 Feb;74(4):1674-85</Citation><ArticleIdList><ArticleId IdType="pubmed">10644337</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2000 Jun 20;272(1):27-39</Citation><ArticleIdList><ArticleId IdType="pubmed">10873746</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2000 Sep 1;19(17):4701-11</Citation><ArticleIdList><ArticleId IdType="pubmed">10970862</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2717-22</Citation><ArticleIdList><ArticleId IdType="pubmed">11226306</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2001 Sep 30;288(2):212-22</Citation><ArticleIdList><ArticleId IdType="pubmed">11601893</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1995 Apr 1;208(1):48-57</Citation><ArticleIdList><ArticleId IdType="pubmed">11831730</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2002 Jun;76(12):6257-67</Citation><ArticleIdList><ArticleId IdType="pubmed">12021359</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2003 Jul 17;312:1-16</Citation><ArticleIdList><ArticleId IdType="pubmed">12909336</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2003 Aug 29;331(5):991-1004</Citation><ArticleIdList><ArticleId IdType="pubmed">12927536</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Mol Cell Biol. 2004 Mar;5(3):232-41</Citation><ArticleIdList><ArticleId IdType="pubmed">14991003</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2004 May;78(10):5288-98</Citation><ArticleIdList><ArticleId IdType="pubmed">15113910</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12694-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15304651</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2004 Oct 29;119(3):381-92</Citation><ArticleIdList><ArticleId IdType="pubmed">15507209</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2004 Nov;78(22):12218-24</Citation><ArticleIdList><ArticleId IdType="pubmed">15507608</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1986 Nov;83(21):8122-6</Citation><ArticleIdList><ArticleId IdType="pubmed">3095828</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1987 Jan;68 ( Pt 1):57-77</Citation><ArticleIdList><ArticleId IdType="pubmed">3027249</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1989 Jun 26;17(12):4847-61</Citation><ArticleIdList><ArticleId IdType="pubmed">2526320</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1991 Dec;185(2):911-7</Citation><ArticleIdList><ArticleId IdType="pubmed">1962461</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1992 Jan;186(1):342-7</Citation><ArticleIdList><ArticleId IdType="pubmed">1309280</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1992 Jul;11(7):2643-54</Citation><ArticleIdList><ArticleId IdType="pubmed">1378397</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1996 Mar;70(3):1923-30</Citation><ArticleIdList><ArticleId IdType="pubmed">8627718</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1997 Mar;71(3):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">9032311</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1998 Apr 10;243(2):388-95</Citation><ArticleIdList><ArticleId IdType="pubmed">9568037</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1998 Jun 5;245(2):303-12</Citation><ArticleIdList><ArticleId IdType="pubmed">9636369</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1998 Jul 5;246(2):288-97</Citation><ArticleIdList><ArticleId IdType="pubmed">9657947</ArticleId></ArticleIdList></Reference><Reference><Citation>Crit Rev Biochem Mol Biol. 1998;33(4):259-96</Citation><ArticleIdList><ArticleId IdType="pubmed">9747670</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Exp Med Biol. 1998;440:173-84</Citation><ArticleIdList><ArticleId IdType="pubmed">9782279</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1999 Jun 18;274(25):17677-83</Citation><ArticleIdList><ArticleId IdType="pubmed">10364207</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2004 Dec 20;330(2):471-80</Citation><ArticleIdList><ArticleId IdType="pubmed">15567440</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Top Microbiol Immunol. 2005;287:31-55</Citation><ArticleIdList><ArticleId IdType="pubmed">15609508</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2005 Jun 5;336(2):299-307</Citation><ArticleIdList><ArticleId IdType="pubmed">15892970</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2007 Feb 5;358(1):136-47</Citation><ArticleIdList><ArticleId IdType="pubmed">16979681</ArticleId></ArticleIdList></Reference><Reference><Citation>Future Microbiol. 2006 Dec;1(4):417-26</Citation><ArticleIdList><ArticleId IdType="pubmed">17661632</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Nov;81(22):12135-44</Citation><ArticleIdList><ArticleId IdType="pubmed">17804504</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2008 May;4(5):e1000054</Citation><ArticleIdList><ArticleId IdType="pubmed">18451981</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Soc Trans. 2008 Aug;36(Pt 4):609-12</Citation><ArticleIdList><ArticleId IdType="pubmed">18631126</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 Sep 11;455(7210):242-5</Citation><ArticleIdList><ArticleId IdType="pubmed">18690214</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Biol. 2008 Sep 16;6(9):e226</Citation><ArticleIdList><ArticleId IdType="pubmed">18798692</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Biochem. 2009 Feb 1;106(2):296-305</Citation><ArticleIdList><ArticleId IdType="pubmed">19058135</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3484-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19208801</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 Jun;83(11):5815-24</Citation><ArticleIdList><ArticleId IdType="pubmed">19297497</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2009 Jun;7(6):439-50</Citation><ArticleIdList><ArticleId IdType="pubmed">19430490</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 Sep;83(17):8744-58</Citation><ArticleIdList><ArticleId IdType="pubmed">19553314</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2010 Jan;91(Pt 1):122-32</Citation><ArticleIdList><ArticleId IdType="pubmed">19793905</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2006 Feb 15;367:17-37</Citation><ArticleIdList><ArticleId IdType="pubmed">16337753</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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