ORF8a of SARS-CoV forms an ion channel: experiments and molecular dynamics simulations.
Identifieur interne : 001649 ( PubMed/Corpus ); précédent : 001648; suivant : 001650ORF8a of SARS-CoV forms an ion channel: experiments and molecular dynamics simulations.
Auteurs : Cheng-Chang Chen ; Jens Krüger ; Issara Sramala ; Hao-Jen Hsu ; Peter Henklein ; Yi-Ming Arthur Chen ; Wolfgang B. FischerSource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 2011.
English descriptors
- KwdEn :
- Amino Acid Sequence, Computer Simulation, Ion Channels (chemistry), Ion Channels (genetics), Lipid Bilayers, Models, Molecular, Molecular Dynamics Simulation, Molecular Sequence Data, Protein Structure, Secondary, SARS Virus (chemistry), SARS Virus (genetics), Viral Matrix Proteins (chemistry), Viral Matrix Proteins (genetics).
- MESH :
- chemical , chemistry : Ion Channels, Viral Matrix Proteins.
- chemical , genetics : Ion Channels, Viral Matrix Proteins.
- chemistry : SARS Virus.
- genetics : SARS Virus.
- Amino Acid Sequence, Computer Simulation, Lipid Bilayers, Models, Molecular, Molecular Dynamics Simulation, Molecular Sequence Data, Protein Structure, Secondary.
Abstract
ORF8a protein is 39 residues long and contains a single transmembrane domain. The protein is synthesized using solid phase peptide synthesis and reconstituted into artificial lipid bilayers that forms cation-selective ion channels with a main conductance level of 8.9±0.8pS at elevated temperature (38.5°C). Computational modeling studies including multi nanosecond molecular dynamics simulations in a hydrated POPC lipid bilayer are done with a 22 amino acid transmembrane helix to predict a putative homooligomeric helical bundle model. A structural model of a pentameric bundle is proposed with cysteines, serines and threonines facing the pore.
DOI: 10.1016/j.bbamem.2010.08.004
PubMed: 20708597
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pubmed:20708597Le document en format XML
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Fischer</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20708597</idno><idno type="pmid">20708597</idno><idno type="doi">10.1016/j.bbamem.2010.08.004</idno><idno type="wicri:Area/PubMed/Corpus">001649</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001649</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">ORF8a of SARS-CoV forms an ion channel: experiments and molecular dynamics simulations.</title><author><name sortKey="Chen, Cheng Chang" sort="Chen, Cheng Chang" uniqKey="Chen C" first="Cheng-Chang" last="Chen">Cheng-Chang Chen</name><affiliation><nlm:affiliation>Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang-Ming University, 155, Li-Nong St., Sec. 2, Taipei, 112, Taiwan.</nlm:affiliation></affiliation></author><author><name sortKey="Kruger, Jens" sort="Kruger, Jens" uniqKey="Kruger J" first="Jens" last="Krüger">Jens Krüger</name></author><author><name sortKey="Sramala, Issara" sort="Sramala, Issara" uniqKey="Sramala I" first="Issara" last="Sramala">Issara Sramala</name></author><author><name sortKey="Hsu, Hao Jen" sort="Hsu, Hao Jen" uniqKey="Hsu H" first="Hao-Jen" last="Hsu">Hao-Jen Hsu</name></author><author><name sortKey="Henklein, Peter" sort="Henklein, Peter" uniqKey="Henklein P" first="Peter" last="Henklein">Peter Henklein</name></author><author><name sortKey="Chen, Yi Ming Arthur" sort="Chen, Yi Ming Arthur" uniqKey="Chen Y" first="Yi-Ming Arthur" last="Chen">Yi-Ming Arthur Chen</name></author><author><name sortKey="Fischer, Wolfgang B" sort="Fischer, Wolfgang B" uniqKey="Fischer W" first="Wolfgang B" last="Fischer">Wolfgang B. Fischer</name></author></analytic><series><title level="j">Biochimica et biophysica acta</title><idno type="ISSN">0006-3002</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Computer Simulation</term><term>Ion Channels (chemistry)</term><term>Ion Channels (genetics)</term><term>Lipid Bilayers</term><term>Models, Molecular</term><term>Molecular Dynamics Simulation</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>Viral Matrix Proteins (chemistry)</term><term>Viral Matrix Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ion Channels</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Ion Channels</term><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Computer Simulation</term><term>Lipid Bilayers</term><term>Models, Molecular</term><term>Molecular Dynamics Simulation</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">ORF8a protein is 39 residues long and contains a single transmembrane domain. The protein is synthesized using solid phase peptide synthesis and reconstituted into artificial lipid bilayers that forms cation-selective ion channels with a main conductance level of 8.9±0.8pS at elevated temperature (38.5°C). Computational modeling studies including multi nanosecond molecular dynamics simulations in a hydrated POPC lipid bilayer are done with a 22 amino acid transmembrane helix to predict a putative homooligomeric helical bundle model. A structural model of a pentameric bundle is proposed with cysteines, serines and threonines facing the pore.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20708597</PMID><DateCompleted><Year>2011</Year><Month>03</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0006-3002</ISSN><JournalIssue CitedMedium="Print"><Volume>1808</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Biochimica et biophysica acta</Title><ISOAbbreviation>Biochim. Biophys. Acta</ISOAbbreviation></Journal><ArticleTitle>ORF8a of SARS-CoV forms an ion channel: experiments and molecular dynamics simulations.</ArticleTitle><Pagination><MedlinePgn>572-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbamem.2010.08.004</ELocationID><Abstract><AbstractText>ORF8a protein is 39 residues long and contains a single transmembrane domain. The protein is synthesized using solid phase peptide synthesis and reconstituted into artificial lipid bilayers that forms cation-selective ion channels with a main conductance level of 8.9±0.8pS at elevated temperature (38.5°C). Computational modeling studies including multi nanosecond molecular dynamics simulations in a hydrated POPC lipid bilayer are done with a 22 amino acid transmembrane helix to predict a putative homooligomeric helical bundle model. A structural model of a pentameric bundle is proposed with cysteines, serines and threonines facing the pore.</AbstractText><CopyrightInformation>2010 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Cheng-Chang</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang-Ming University, 155, Li-Nong St., Sec. 2, Taipei, 112, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krüger</LastName><ForeName>Jens</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Sramala</LastName><ForeName>Issara</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Hsu</LastName><ForeName>Hao-Jen</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Henklein</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yi-Ming Arthur</ForeName><Initials>YM</Initials></Author><Author ValidYN="Y"><LastName>Fischer</LastName><ForeName>Wolfgang B</ForeName><Initials>WB</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>08</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biochim Biophys Acta</MedlineTA><NlmUniqueID>0217513</NlmUniqueID><ISSNLinking>0006-3002</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007473">Ion Channels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008051">Lipid Bilayers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014763">Viral Matrix Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017433" MajorTopicYN="N">Protein Structure, Secondary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014763" MajorTopicYN="N">Viral Matrix Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>06</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>07</Month><Day>27</Day></PubMedPubDate><PubMedPubDate 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