Serveur d'exploration SRAS

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.

Identifieur interne : 001646 ( PubMed/Corpus ); précédent : 001645; suivant : 001647

Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.

Auteurs : Shigeru Kyuwa ; Satoshi Takagaki ; Shutoku Matsuyama ; Fumihiro Taguchi ; Junzo Saegusa ; Yoichiroh Iwakura ; Yoh-Ichi Tagawa ; Yasuhiro Yoshikawa

Source :

RBID : pubmed:20712488

English descriptors

Abstract

Previously, we showed that intraperitoneal infection with murine coronavirus strain JHM (JHMV) established a persistent infection with subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 (B6-GKO) mice. Herein, we characterize a variant virus from B6-GKO mice persistently infected with JHMV. Viruses were isolated from ascites at 25 d post-infection and cloned by limiting dilution on DBT cells; one variant was named 25V16G. To compare pathogenicity in vivo, we inoculated 25V16G and JHMV intraperitoneally into 8- to 12-week-old B6-GKO mice. Whereas nearly all of the B6-GKO mice infected with JHMV survived over 14 d, all of those infected with 25V16G died by 9 d post-infection. Histopathological examination revealed that 25V16G induced acute fulminant hepatitis in B6-GKO mice, whereas JHMV caused severe but focal hepatitis. The virus titer of 25V16G in the liver was 50- and 250-fold higher than that of JHMV at 5 and 7 d post-infection, respectively. However, there was no significant difference in viral growth between 25V16G and JHMV in cell lines cultured in vitro. Nucleotide sequencing of the S gene of 25V16G and JHMV revealed a deletion of 29 amino acids encompassing S(511-539), which covers a major cytotoxic T lymphocyte (CTL) epitope in C57BL/6 mice, and two point mutations resulting in amino acid changes in the S protein of 25V16G. One explanation for the greater pathogenicity of 25V16G is that 25V16G escapes CTL-mediated protection in B6-GKO mice. This experimental model may be used to assess the role of IFN-gamma in viral persistence in vivo.

DOI: 10.1089/vim.2010.0008
PubMed: 20712488

Links to Exploration step

pubmed:20712488

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.</title>
<author>
<name sortKey="Kyuwa, Shigeru" sort="Kyuwa, Shigeru" uniqKey="Kyuwa S" first="Shigeru" last="Kyuwa">Shigeru Kyuwa</name>
<affiliation>
<nlm:affiliation>Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. akyuwa@mail.ecc.u-tokyo.ac.jp</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Takagaki, Satoshi" sort="Takagaki, Satoshi" uniqKey="Takagaki S" first="Satoshi" last="Takagaki">Satoshi Takagaki</name>
</author>
<author>
<name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name>
</author>
<author>
<name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
</author>
<author>
<name sortKey="Saegusa, Junzo" sort="Saegusa, Junzo" uniqKey="Saegusa J" first="Junzo" last="Saegusa">Junzo Saegusa</name>
</author>
<author>
<name sortKey="Iwakura, Yoichiroh" sort="Iwakura, Yoichiroh" uniqKey="Iwakura Y" first="Yoichiroh" last="Iwakura">Yoichiroh Iwakura</name>
</author>
<author>
<name sortKey="Tagawa, Yoh Ichi" sort="Tagawa, Yoh Ichi" uniqKey="Tagawa Y" first="Yoh-Ichi" last="Tagawa">Yoh-Ichi Tagawa</name>
</author>
<author>
<name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, Yasuhiro" uniqKey="Yoshikawa Y" first="Yasuhiro" last="Yoshikawa">Yasuhiro Yoshikawa</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20712488</idno>
<idno type="pmid">20712488</idno>
<idno type="doi">10.1089/vim.2010.0008</idno>
<idno type="wicri:Area/PubMed/Corpus">001646</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001646</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en">Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.</title>
<author>
<name sortKey="Kyuwa, Shigeru" sort="Kyuwa, Shigeru" uniqKey="Kyuwa S" first="Shigeru" last="Kyuwa">Shigeru Kyuwa</name>
<affiliation>
<nlm:affiliation>Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. akyuwa@mail.ecc.u-tokyo.ac.jp</nlm:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Takagaki, Satoshi" sort="Takagaki, Satoshi" uniqKey="Takagaki S" first="Satoshi" last="Takagaki">Satoshi Takagaki</name>
</author>
<author>
<name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name>
</author>
<author>
<name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
</author>
<author>
<name sortKey="Saegusa, Junzo" sort="Saegusa, Junzo" uniqKey="Saegusa J" first="Junzo" last="Saegusa">Junzo Saegusa</name>
</author>
<author>
<name sortKey="Iwakura, Yoichiroh" sort="Iwakura, Yoichiroh" uniqKey="Iwakura Y" first="Yoichiroh" last="Iwakura">Yoichiroh Iwakura</name>
</author>
<author>
<name sortKey="Tagawa, Yoh Ichi" sort="Tagawa, Yoh Ichi" uniqKey="Tagawa Y" first="Yoh-Ichi" last="Tagawa">Yoh-Ichi Tagawa</name>
</author>
<author>
<name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, Yasuhiro" uniqKey="Yoshikawa Y" first="Yasuhiro" last="Yoshikawa">Yasuhiro Yoshikawa</name>
</author>
</analytic>
<series>
<title level="j">Viral immunology</title>
<idno type="eISSN">1557-8976</idno>
<imprint>
<date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Animals</term>
<term>Ascitic Fluid (virology)</term>
<term>Female</term>
<term>Genetic Variation</term>
<term>Hepatitis, Viral, Animal (immunology)</term>
<term>Hepatitis, Viral, Animal (pathology)</term>
<term>Hepatitis, Viral, Animal (virology)</term>
<term>Interferon-gamma (deficiency)</term>
<term>Interferon-gamma (genetics)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Mice, Knockout</term>
<term>Murine hepatitis virus (genetics)</term>
<term>Murine hepatitis virus (pathogenicity)</term>
<term>Point Mutation</term>
<term>Serositis (pathology)</term>
<term>Serositis (virology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en">
<term>Interferon-gamma</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>Interferon-gamma</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en">
<term>Hepatitis, Viral, Animal</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en">
<term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en">
<term>Hepatitis, Viral, Animal</term>
<term>Serositis</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en">
<term>Ascitic Fluid</term>
<term>Hepatitis, Viral, Animal</term>
<term>Serositis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Animals</term>
<term>Female</term>
<term>Genetic Variation</term>
<term>Mice</term>
<term>Mice, Inbred C57BL</term>
<term>Mice, Knockout</term>
<term>Point Mutation</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Virulence</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">Previously, we showed that intraperitoneal infection with murine coronavirus strain JHM (JHMV) established a persistent infection with subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 (B6-GKO) mice. Herein, we characterize a variant virus from B6-GKO mice persistently infected with JHMV. Viruses were isolated from ascites at 25 d post-infection and cloned by limiting dilution on DBT cells; one variant was named 25V16G. To compare pathogenicity in vivo, we inoculated 25V16G and JHMV intraperitoneally into 8- to 12-week-old B6-GKO mice. Whereas nearly all of the B6-GKO mice infected with JHMV survived over 14 d, all of those infected with 25V16G died by 9 d post-infection. Histopathological examination revealed that 25V16G induced acute fulminant hepatitis in B6-GKO mice, whereas JHMV caused severe but focal hepatitis. The virus titer of 25V16G in the liver was 50- and 250-fold higher than that of JHMV at 5 and 7 d post-infection, respectively. However, there was no significant difference in viral growth between 25V16G and JHMV in cell lines cultured in vitro. Nucleotide sequencing of the S gene of 25V16G and JHMV revealed a deletion of 29 amino acids encompassing S(511-539), which covers a major cytotoxic T lymphocyte (CTL) epitope in C57BL/6 mice, and two point mutations resulting in amino acid changes in the S protein of 25V16G. One explanation for the greater pathogenicity of 25V16G is that 25V16G escapes CTL-mediated protection in B6-GKO mice. This experimental model may be used to assess the role of IFN-gamma in viral persistence in vivo.</div>
</front>
</TEI>
<pubmed>
<MedlineCitation Status="MEDLINE" Owner="NLM">
<PMID Version="1">20712488</PMID>
<DateCompleted>
<Year>2011</Year>
<Month>01</Month>
<Day>26</Day>
</DateCompleted>
<DateRevised>
<Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print">
<Journal>
<ISSN IssnType="Electronic">1557-8976</ISSN>
<JournalIssue CitedMedium="Internet">
<Volume>23</Volume>
<Issue>4</Issue>
<PubDate>
<Year>2010</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Viral immunology</Title>
<ISOAbbreviation>Viral Immunol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.</ArticleTitle>
<Pagination>
<MedlinePgn>437-42</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1089/vim.2010.0008</ELocationID>
<Abstract>
<AbstractText>Previously, we showed that intraperitoneal infection with murine coronavirus strain JHM (JHMV) established a persistent infection with subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 (B6-GKO) mice. Herein, we characterize a variant virus from B6-GKO mice persistently infected with JHMV. Viruses were isolated from ascites at 25 d post-infection and cloned by limiting dilution on DBT cells; one variant was named 25V16G. To compare pathogenicity in vivo, we inoculated 25V16G and JHMV intraperitoneally into 8- to 12-week-old B6-GKO mice. Whereas nearly all of the B6-GKO mice infected with JHMV survived over 14 d, all of those infected with 25V16G died by 9 d post-infection. Histopathological examination revealed that 25V16G induced acute fulminant hepatitis in B6-GKO mice, whereas JHMV caused severe but focal hepatitis. The virus titer of 25V16G in the liver was 50- and 250-fold higher than that of JHMV at 5 and 7 d post-infection, respectively. However, there was no significant difference in viral growth between 25V16G and JHMV in cell lines cultured in vitro. Nucleotide sequencing of the S gene of 25V16G and JHMV revealed a deletion of 29 amino acids encompassing S(511-539), which covers a major cytotoxic T lymphocyte (CTL) epitope in C57BL/6 mice, and two point mutations resulting in amino acid changes in the S protein of 25V16G. One explanation for the greater pathogenicity of 25V16G is that 25V16G escapes CTL-mediated protection in B6-GKO mice. This experimental model may be used to assess the role of IFN-gamma in viral persistence in vivo.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y">
<Author ValidYN="Y">
<LastName>Kyuwa</LastName>
<ForeName>Shigeru</ForeName>
<Initials>S</Initials>
<AffiliationInfo>
<Affiliation>Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. akyuwa@mail.ecc.u-tokyo.ac.jp</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Takagaki</LastName>
<ForeName>Satoshi</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Matsuyama</LastName>
<ForeName>Shutoku</ForeName>
<Initials>S</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Taguchi</LastName>
<ForeName>Fumihiro</ForeName>
<Initials>F</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Saegusa</LastName>
<ForeName>Junzo</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Iwakura</LastName>
<ForeName>Yoichiroh</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Tagawa</LastName>
<ForeName>Yoh-Ichi</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Yoshikawa</LastName>
<ForeName>Yasuhiro</ForeName>
<Initials>Y</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo>
<Country>United States</Country>
<MedlineTA>Viral Immunol</MedlineTA>
<NlmUniqueID>8801552</NlmUniqueID>
<ISSNLinking>0882-8245</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>82115-62-6</RegistryNumber>
<NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<CommentsCorrectionsList>
<CommentsCorrections RefType="CommentIn">
<RefSource>Viral Immunol. 2010 Aug;23(4):341</RefSource>
<PMID Version="1">20712477</PMID>
</CommentsCorrections>
</CommentsCorrectionsList>
<MeshHeadingList>
<MeshHeading>
<DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D001202" MajorTopicYN="N">Ascitic Fluid</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D006524" MajorTopicYN="N">Hepatitis, Viral, Animal</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName>
<QualifierName UI="Q000172" MajorTopicYN="N">deficiency</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D018345" MajorTopicYN="N">Mice, Knockout</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D006517" MajorTopicYN="N">Murine hepatitis virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D017354" MajorTopicYN="N">Point Mutation</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D012700" MajorTopicYN="N">Serositis</DescriptorName>
<QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData>
<History>
<PubMedPubDate PubStatus="entrez">
<Year>2010</Year>
<Month>8</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed">
<Year>2010</Year>
<Month>8</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline">
<Year>2011</Year>
<Month>1</Month>
<Day>28</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList>
<ArticleId IdType="pubmed">20712488</ArticleId>
<ArticleId IdType="doi">10.1089/vim.2010.0008</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001646 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 001646 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PubMed
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:20712488
   |texte=   Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i   -Sk "pubmed:20712488" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1 

Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021