Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice.
Identifieur interne : 001633 ( PubMed/Corpus ); précédent : 001632; suivant : 001634Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice.
Auteurs : Kazuya Shirato ; Madoka Maejima ; Asuka Hirai ; Yasushi Ami ; Natsumi Takeyama ; Kotaro Tsuchiya ; Kouich Kusanagi ; Tetsuo Nunoya ; Fumihiro TaguchiSource :
- Archives of virology [ 1432-8798 ] ; 2010.
English descriptors
- KwdEn :
- Adaptation, Biological, Amino Acid Substitution (genetics), Animals, Brain (virology), Cell Fusion, Cells, Cultured, Disease Models, Animal, Female, Membrane Glycoproteins (genetics), Mice, Mice, Inbred ICR, Porcine epidemic diarrhea virus (genetics), Porcine epidemic diarrhea virus (pathogenicity), Pregnancy, Serial Passage, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Virulence.
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Amino Acid Substitution, Porcine epidemic diarrhea virus.
- pathogenicity : Porcine epidemic diarrhea virus.
- virology : Brain.
- Adaptation, Biological, Animals, Cell Fusion, Cells, Cultured, Disease Models, Animal, Female, Mice, Mice, Inbred ICR, Pregnancy, Serial Passage, Spike Glycoprotein, Coronavirus, Virulence.
Abstract
Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.
DOI: 10.1007/s00705-010-0790-1
PubMed: 20827493
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pubmed:20827493Le document en format XML
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last="Takeyama">Natsumi Takeyama</name></author><author><name sortKey="Tsuchiya, Kotaro" sort="Tsuchiya, Kotaro" uniqKey="Tsuchiya K" first="Kotaro" last="Tsuchiya">Kotaro Tsuchiya</name></author><author><name sortKey="Kusanagi, Kouich" sort="Kusanagi, Kouich" uniqKey="Kusanagi K" first="Kouich" last="Kusanagi">Kouich Kusanagi</name></author><author><name sortKey="Nunoya, Tetsuo" sort="Nunoya, Tetsuo" uniqKey="Nunoya T" first="Tetsuo" last="Nunoya">Tetsuo Nunoya</name></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20827493</idno><idno type="pmid">20827493</idno><idno type="doi">10.1007/s00705-010-0790-1</idno><idno type="wicri:Area/PubMed/Corpus">001633</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" 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uniqKey="Takeyama N" first="Natsumi" last="Takeyama">Natsumi Takeyama</name></author><author><name sortKey="Tsuchiya, Kotaro" sort="Tsuchiya, Kotaro" uniqKey="Tsuchiya K" first="Kotaro" last="Tsuchiya">Kotaro Tsuchiya</name></author><author><name sortKey="Kusanagi, Kouich" sort="Kusanagi, Kouich" uniqKey="Kusanagi K" first="Kouich" last="Kusanagi">Kouich Kusanagi</name></author><author><name sortKey="Nunoya, Tetsuo" sort="Nunoya, Tetsuo" uniqKey="Nunoya T" first="Tetsuo" last="Nunoya">Tetsuo Nunoya</name></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></author></analytic><series><title level="j">Archives of virology</title><idno type="eISSN">1432-8798</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Biological</term><term>Amino Acid Substitution (genetics)</term><term>Animals</term><term>Brain (virology)</term><term>Cell Fusion</term><term>Cells, Cultured</term><term>Disease Models, Animal</term><term>Female</term><term>Membrane Glycoproteins (genetics)</term><term>Mice</term><term>Mice, Inbred ICR</term><term>Porcine epidemic diarrhea virus (genetics)</term><term>Porcine epidemic diarrhea virus (pathogenicity)</term><term>Pregnancy</term><term>Serial Passage</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Virulence</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Amino Acid Substitution</term><term>Porcine epidemic diarrhea virus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Porcine epidemic diarrhea virus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Brain</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Biological</term><term>Animals</term><term>Cell Fusion</term><term>Cells, Cultured</term><term>Disease Models, Animal</term><term>Female</term><term>Mice</term><term>Mice, Inbred ICR</term><term>Pregnancy</term><term>Serial Passage</term><term>Spike Glycoprotein, Coronavirus</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20827493</PMID><DateCompleted><Year>2010</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-8798</ISSN><JournalIssue CitedMedium="Internet"><Volume>155</Volume><Issue>12</Issue><PubDate><Year>2010</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Archives of virology</Title><ISOAbbreviation>Arch. Virol.</ISOAbbreviation></Journal><ArticleTitle>Enhanced cell fusion activity in porcine epidemic diarrhea virus adapted to suckling mice.</ArticleTitle><Pagination><MedlinePgn>1989-95</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00705-010-0790-1</ELocationID><Abstract><AbstractText>Porcine epidemic diarrhea virus (PEDV) is the major causative agent of fatal diarrhea in piglets. To study the pathogenic features of PEDV using a mouse model, PEDV with virulence in mice is required. In pursuit of this, we adapted a tissue-culture-passed PEDV MK strain to suckling mouse brains. PEDV obtained after ten passages through the brains (MK-p10) had increased virulence for mice, and its fusion activity in cultured cells exceeded that of the original strain. However, the replication kinetics of MK and MK-p10 did not differ from each other in the brain and in cultured cells. The spike (S) protein of MK-p10 had four amino acid substitutions relative to the original strain. One of these (an H-to-R substitution at residue 1,381) was first detected in PEDV isolated after eight passages, and both this virus (MK-p8) and MK-p10 showed enhanced syncytium formation relative to the original MK strain and viruses isolated after two, four, and six passages, suggesting the possibility that the H-to-R mutation was responsible for this activity. This mutation could be also involved in the increased virulence of PEDV observed for MK-p10.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shirato</LastName><ForeName>Kazuya</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Virology III, Laboratory of Acute Respiratory Viral Diseases and Cytokines, Musashimurayama, Tokyo, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maejima</LastName><ForeName>Madoka</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Hirai</LastName><ForeName>Asuka</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ami</LastName><ForeName>Yasushi</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Takeyama</LastName><ForeName>Natsumi</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Tsuchiya</LastName><ForeName>Kotaro</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Kusanagi</LastName><ForeName>Kouich</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Nunoya</LastName><ForeName>Tetsuo</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Taguchi</LastName><ForeName>Fumihiro</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>09</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Arch Virol</MedlineTA><NlmUniqueID>7506870</NlmUniqueID><ISSNLinking>0304-8608</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000220" MajorTopicYN="Y">Adaptation, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019943" MajorTopicYN="N">Amino Acid Substitution</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" 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