SrasV1, PubMed, Corpus, bibRecord, 001482

The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

Identifieur interne : 001482 ( PubMed/Corpus ); précédent : 001481; suivant : 001483

The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

Auteurs : Jessica Celigoy ; Susanna Mcreynolds ; Michael Caffrey

Source :

Biochemical and biophysical research communications [ 1090-2104 ] ; 2011.

RBID : pubmed:21835164

English descriptors

KwdEn :
- Circular Dichroism, Hydrogen-Ion Concentration, Protein Structure, Secondary, Repetitive Sequences, Amino Acid, SARS Virus (chemistry), Static Electricity, Virus Internalization.
MESH :
- chemistry : SARS Virus.
- Circular Dichroism, Hydrogen-Ion Concentration, Protein Structure, Secondary, Repetitive Sequences, Amino Acid, Static Electricity, Virus Internalization.

Abstract

The heptad repeats 1 and 2 of SARS-CoV spike, termed HR1 and HR2, play critical roles in viral entry. Moreover, HR1 and HR2 derived free peptides are inhibitors of SARS-CoV entry. In this work we used circular dichroism to show that HR2 helix formation is induced at pH 5, the pH of the endosome. In addition, we demonstrate that the HR2 helix is further stabilized at physiological ionic strengths. Together, these observations provide new insight into the mechanism of SARS-CoV entry and suggest that HR2 may be an attractive target for therapeutic intervention.

DOI: 10.1016/j.bbrc.2011.07.126
PubMed: 21835164

Links to Exploration step

pubmed:21835164

Le document en format XML

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<author><name sortKey="Celigoy, Jessica" sort="Celigoy, Jessica" uniqKey="Celigoy J" first="Jessica" last="Celigoy">Jessica Celigoy</name>
<affiliation><nlm:affiliation>Department of Biochemistry & Molecular Genetics University of Illinois at Chicago, Chicago, IL 60607, USA.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Mcreynolds, Susanna" sort="Mcreynolds, Susanna" uniqKey="Mcreynolds S" first="Susanna" last="Mcreynolds">Susanna Mcreynolds</name>
</author>
<author><name sortKey="Caffrey, Michael" sort="Caffrey, Michael" uniqKey="Caffrey M" first="Michael" last="Caffrey">Michael Caffrey</name>
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<author><name sortKey="Mcreynolds, Susanna" sort="Mcreynolds, Susanna" uniqKey="Mcreynolds S" first="Susanna" last="Mcreynolds">Susanna Mcreynolds</name>
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<series><title level="j">Biochemical and biophysical research communications</title>
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<term>Hydrogen-Ion Concentration</term>
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<term>Repetitive Sequences, Amino Acid</term>
<term>SARS Virus (chemistry)</term>
<term>Static Electricity</term>
<term>Virus Internalization</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" xml:lang="en"><term>Circular Dichroism</term>
<term>Hydrogen-Ion Concentration</term>
<term>Protein Structure, Secondary</term>
<term>Repetitive Sequences, Amino Acid</term>
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<front><div type="abstract" xml:lang="en">The heptad repeats 1 and 2 of SARS-CoV spike, termed HR1 and HR2, play critical roles in viral entry. Moreover, HR1 and HR2 derived free peptides are inhibitors of SARS-CoV entry. In this work we used circular dichroism to show that HR2 helix formation is induced at pH 5, the pH of the endosome. In addition, we demonstrate that the HR2 helix is further stabilized at physiological ionic strengths. Together, these observations provide new insight into the mechanism of SARS-CoV entry and suggest that HR2 may be an attractive target for therapeutic intervention.</div>
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<Title>Biochemical and biophysical research communications</Title>
<ISOAbbreviation>Biochem. Biophys. Res. Commun.</ISOAbbreviation>
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<ArticleTitle>The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.</ArticleTitle>
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<Abstract><AbstractText>The heptad repeats 1 and 2 of SARS-CoV spike, termed HR1 and HR2, play critical roles in viral entry. Moreover, HR1 and HR2 derived free peptides are inhibitors of SARS-CoV entry. In this work we used circular dichroism to show that HR2 helix formation is induced at pH 5, the pH of the endosome. In addition, we demonstrate that the HR2 helix is further stabilized at physiological ionic strengths. Together, these observations provide new insight into the mechanism of SARS-CoV entry and suggest that HR2 may be an attractive target for therapeutic intervention.</AbstractText>
<CopyrightInformation>Copyright © 2011 Elsevier Inc. All rights reserved.</CopyrightInformation>
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The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

Source :

English descriptors

Abstract

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