Phosphatidylinositol 4-kinase IIIβ is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry.
Identifieur interne : 001418 ( PubMed/Corpus ); précédent : 001417; suivant : 001419Phosphatidylinositol 4-kinase IIIβ is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry.
Auteurs : Ning Yang ; Ping Ma ; Jianshe Lang ; Yanli Zhang ; Jiejie Deng ; Xiangwu Ju ; Gongyi Zhang ; Chengyu JiangSource :
- The Journal of biological chemistry [ 1083-351X ] ; 2012.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Enzyme Inhibitors (chemistry), Enzyme Inhibitors (pharmacology), HEK293 Cells, Humans, Membrane Lipids (metabolism), Minor Histocompatibility Antigens, Phosphotransferases (Alcohol Group Acceptor) (antagonists & inhibitors), Phosphotransferases (Alcohol Group Acceptor) (metabolism), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (drug therapy), Severe Acute Respiratory Syndrome (metabolism), Virion (metabolism), Virus Internalization.
- MESH :
- chemical , antagonists & inhibitors : Phosphotransferases (Alcohol Group Acceptor).
- chemical , chemistry : Enzyme Inhibitors.
- chemical , metabolism : Membrane Lipids, Phosphotransferases (Alcohol Group Acceptor).
- chemical , pharmacology : Enzyme Inhibitors.
- drug therapy : Severe Acute Respiratory Syndrome.
- metabolism : SARS Virus, Severe Acute Respiratory Syndrome, Virion.
- Animals, Chlorocebus aethiops, HEK293 Cells, Humans, Minor Histocompatibility Antigens, Virus Internalization.
Abstract
Phosphatidylinositol kinases (PI kinases) play an important role in the life cycle of several viruses after infection. Using gene knockdown technology, we demonstrate that phosphatidylinositol 4-kinase IIIβ (PI4KB) is required for cellular entry by pseudoviruses bearing the severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein and that the cell entry mediated by SARS-CoV spike protein is strongly inhibited by knockdown of PI4KB. Consistent with this observation, pharmacological inhibitors of PI4KB blocked entry of SARS pseudovirions. Further research suggested that PI4P plays an essential role in SARS-CoV spike-mediated entry, which is regulated by the PI4P lipid microenvironment. We further demonstrate that PI4KB does not affect virus entry at the SARS-CoV S-ACE2 binding interface or at the stage of virus internalization but rather at or before virus fusion. Taken together, these results indicate a new function for PI4KB and suggest a new drug target for preventing SARS-CoV infection.
DOI: 10.1074/jbc.M111.312561
PubMed: 22253445
Links to Exploration step
pubmed:22253445Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Phosphatidylinositol 4-kinase IIIβ is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry.</title><author><name sortKey="Yang, Ning" sort="Yang, Ning" uniqKey="Yang N" first="Ning" last="Yang">Ning Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University, Chinese Academy of Medical Sciences, Beijing 100005, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Ping" sort="Ma, Ping" uniqKey="Ma P" first="Ping" last="Ma">Ping Ma</name></author><author><name sortKey="Lang, Jianshe" sort="Lang, Jianshe" uniqKey="Lang J" first="Jianshe" last="Lang">Jianshe Lang</name></author><author><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name></author><author><name sortKey="Deng, Jiejie" sort="Deng, Jiejie" uniqKey="Deng J" first="Jiejie" last="Deng">Jiejie Deng</name></author><author><name sortKey="Ju, Xiangwu" sort="Ju, Xiangwu" uniqKey="Ju X" first="Xiangwu" last="Ju">Xiangwu Ju</name></author><author><name sortKey="Zhang, Gongyi" sort="Zhang, Gongyi" uniqKey="Zhang G" first="Gongyi" last="Zhang">Gongyi Zhang</name></author><author><name sortKey="Jiang, Chengyu" sort="Jiang, Chengyu" uniqKey="Jiang C" first="Chengyu" last="Jiang">Chengyu Jiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22253445</idno><idno type="pmid">22253445</idno><idno type="doi">10.1074/jbc.M111.312561</idno><idno type="wicri:Area/PubMed/Corpus">001418</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001418</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Phosphatidylinositol 4-kinase IIIβ is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry.</title><author><name sortKey="Yang, Ning" sort="Yang, Ning" uniqKey="Yang N" first="Ning" last="Yang">Ning Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University, Chinese Academy of Medical Sciences, Beijing 100005, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Ping" sort="Ma, Ping" uniqKey="Ma P" first="Ping" last="Ma">Ping Ma</name></author><author><name sortKey="Lang, Jianshe" sort="Lang, Jianshe" uniqKey="Lang J" first="Jianshe" last="Lang">Jianshe Lang</name></author><author><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name></author><author><name sortKey="Deng, Jiejie" sort="Deng, Jiejie" uniqKey="Deng J" first="Jiejie" last="Deng">Jiejie Deng</name></author><author><name sortKey="Ju, Xiangwu" sort="Ju, Xiangwu" uniqKey="Ju X" first="Xiangwu" last="Ju">Xiangwu Ju</name></author><author><name sortKey="Zhang, Gongyi" sort="Zhang, Gongyi" uniqKey="Zhang G" first="Gongyi" last="Zhang">Gongyi Zhang</name></author><author><name sortKey="Jiang, Chengyu" sort="Jiang, Chengyu" uniqKey="Jiang C" first="Chengyu" last="Jiang">Chengyu Jiang</name></author></analytic><series><title level="j">The Journal of biological chemistry</title><idno type="eISSN">1083-351X</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Enzyme Inhibitors (chemistry)</term><term>Enzyme Inhibitors (pharmacology)</term><term>HEK293 Cells</term><term>Humans</term><term>Membrane Lipids (metabolism)</term><term>Minor Histocompatibility Antigens</term><term>Phosphotransferases (Alcohol Group Acceptor) (antagonists & inhibitors)</term><term>Phosphotransferases (Alcohol Group Acceptor) (metabolism)</term><term>SARS Virus (metabolism)</term><term>Severe Acute Respiratory Syndrome (drug therapy)</term><term>Severe Acute Respiratory Syndrome (metabolism)</term><term>Virion (metabolism)</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Phosphotransferases (Alcohol Group Acceptor)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Lipids</term><term>Phosphotransferases (Alcohol Group Acceptor)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>Virion</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>HEK293 Cells</term><term>Humans</term><term>Minor Histocompatibility Antigens</term><term>Virus Internalization</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phosphatidylinositol kinases (PI kinases) play an important role in the life cycle of several viruses after infection. Using gene knockdown technology, we demonstrate that phosphatidylinositol 4-kinase IIIβ (PI4KB) is required for cellular entry by pseudoviruses bearing the severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein and that the cell entry mediated by SARS-CoV spike protein is strongly inhibited by knockdown of PI4KB. Consistent with this observation, pharmacological inhibitors of PI4KB blocked entry of SARS pseudovirions. Further research suggested that PI4P plays an essential role in SARS-CoV spike-mediated entry, which is regulated by the PI4P lipid microenvironment. We further demonstrate that PI4KB does not affect virus entry at the SARS-CoV S-ACE2 binding interface or at the stage of virus internalization but rather at or before virus fusion. Taken together, these results indicate a new function for PI4KB and suggest a new drug target for preventing SARS-CoV infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22253445</PMID><DateCompleted><Year>2012</Year><Month>05</Month><Day>01</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1083-351X</ISSN><JournalIssue CitedMedium="Internet"><Volume>287</Volume><Issue>11</Issue><PubDate><Year>2012</Year><Month>Mar</Month><Day>09</Day></PubDate></JournalIssue><Title>The Journal of biological chemistry</Title><ISOAbbreviation>J. Biol. Chem.</ISOAbbreviation></Journal><ArticleTitle>Phosphatidylinositol 4-kinase IIIβ is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry.</ArticleTitle><Pagination><MedlinePgn>8457-67</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1074/jbc.M111.312561</ELocationID><Abstract><AbstractText>Phosphatidylinositol kinases (PI kinases) play an important role in the life cycle of several viruses after infection. Using gene knockdown technology, we demonstrate that phosphatidylinositol 4-kinase IIIβ (PI4KB) is required for cellular entry by pseudoviruses bearing the severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein and that the cell entry mediated by SARS-CoV spike protein is strongly inhibited by knockdown of PI4KB. Consistent with this observation, pharmacological inhibitors of PI4KB blocked entry of SARS pseudovirions. Further research suggested that PI4P plays an essential role in SARS-CoV spike-mediated entry, which is regulated by the PI4P lipid microenvironment. We further demonstrate that PI4KB does not affect virus entry at the SARS-CoV S-ACE2 binding interface or at the stage of virus internalization but rather at or before virus fusion. Taken together, these results indicate a new function for PI4KB and suggest a new drug target for preventing SARS-CoV infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Ning</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University, Chinese Academy of Medical Sciences, Beijing 100005, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Ping</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Lang</LastName><ForeName>Jianshe</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yanli</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Jiejie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ju</LastName><ForeName>Xiangwu</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Gongyi</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Chengyu</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>01</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Biol Chem</MedlineTA><NlmUniqueID>2985121R</NlmUniqueID><ISSNLinking>0021-9258</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008563">Membrane Lipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015778">Minor Histocompatibility Antigens</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.1.-</RegistryNumber><NameOfSubstance UI="D017853">Phosphotransferases (Alcohol Group Acceptor)</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.1.67</RegistryNumber><NameOfSubstance UI="C402182">phosphatidylinositol phosphate 4-kinase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008563" MajorTopicYN="N">Membrane Lipids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015778" MajorTopicYN="N">Minor Histocompatibility Antigens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017853" MajorTopicYN="N">Phosphotransferases (Alcohol Group Acceptor)</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014771" MajorTopicYN="N">Virion</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053586" MajorTopicYN="Y">Virus Internalization</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22253445</ArticleId><ArticleId IdType="pii">M111.312561</ArticleId><ArticleId IdType="doi">10.1074/jbc.M111.312561</ArticleId><ArticleId IdType="pmc">PMC3318727</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Mol Pharmacol. 2010 Aug;78(2):319-24</Citation><ArticleIdList><ArticleId IdType="pubmed">20466822</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2010 May 28;141(5):799-811</Citation><ArticleIdList><ArticleId IdType="pubmed">20510927</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2011 Jan 20;9(1):32-45</Citation><ArticleIdList><ArticleId IdType="pubmed">21238945</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2011;7(1):e1001258</Citation><ArticleIdList><ArticleId IdType="pubmed">21253575</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2011 Apr 1;286(13):11290-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21297162</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(8):e23710</Citation><ArticleIdList><ArticleId IdType="pubmed">21887302</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 May 5;106(18):7577-82</Citation><ArticleIdList><ArticleId IdType="pubmed">19376974</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene Ther. 2000 Oct;7(19):1613-23</Citation><ArticleIdList><ArticleId IdType="pubmed">11083469</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Sci. 2003 Aug 1;116(Pt 15):3037-40</Citation><ArticleIdList><ArticleId IdType="pubmed">12829733</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Virol. 2003 Nov;71(3):323-31</Citation><ArticleIdList><ArticleId IdType="pubmed">12966536</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4240-5</Citation><ArticleIdList><ArticleId IdType="pubmed">15010527</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2004 Jun;78(11):5642-50</Citation><ArticleIdList><ArticleId IdType="pubmed">15140961</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Virol. 2004 Jul;73(3):332-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15170625</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2004 Oct;78(19):10628-35</Citation><ArticleIdList><ArticleId IdType="pubmed">15367630</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1990 Nov 15;265(32):19704-11</Citation><ArticleIdList><ArticleId IdType="pubmed">2174051</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Biochem. 1998;67:481-507</Citation><ArticleIdList><ArticleId IdType="pubmed">9759495</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2005 Aug;11(8):875-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16007097</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11876-81</Citation><ArticleIdList><ArticleId IdType="pubmed">16081529</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2006 Feb 10;281(6):3198-203</Citation><ArticleIdList><ArticleId IdType="pubmed">16339146</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 Feb 24;124(4):729-40</Citation><ArticleIdList><ArticleId IdType="pubmed">16497584</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Cell Biol. 2006 Jul;16(7):351-61</Citation><ArticleIdList><ArticleId IdType="pubmed">16793271</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Apr;81(7):3058-67</Citation><ArticleIdList><ArticleId IdType="pubmed">17229704</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2008 Jan;10(1):122-33</Citation><ArticleIdList><ArticleId IdType="pubmed">18086046</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2008 Feb;18(2):290-301</Citation><ArticleIdList><ArticleId IdType="pubmed">18227861</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Biol. 2008 Feb 25;180(4):803-12</Citation><ArticleIdList><ArticleId IdType="pubmed">18299350</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Sci. 2008 Jun 15;121(Pt 12):1955-63</Citation><ArticleIdList><ArticleId IdType="pubmed">18525025</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7809-14</Citation><ArticleIdList><ArticleId IdType="pubmed">18490652</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2008 Sep;136(1-2):8-15</Citation><ArticleIdList><ArticleId IdType="pubmed">18554741</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2008 Sep;82(17):8887-90</Citation><ArticleIdList><ArticleId IdType="pubmed">18562523</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2008;4(8):e1000141</Citation><ArticleIdList><ArticleId IdType="pubmed">18769720</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2008 Oct 8;27(19):2457-70</Citation><ArticleIdList><ArticleId IdType="pubmed">18784754</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2009 Mar 19;5(3):298-307</Citation><ArticleIdList><ArticleId IdType="pubmed">19286138</ArticleId></ArticleIdList></Reference><Reference><Citation>FASEB J. 2009 Nov;23(11):3780-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19608626</ArticleId></ArticleIdList></Reference><Reference><Citation>Antiviral Res. 2010 Mar;85(3):551-5</Citation><ArticleIdList><ArticleId IdType="pubmed">19995578</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2011 Jan;85(1):422-31</Citation><ArticleIdList><ArticleId IdType="pubmed">20980511</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001418 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 001418 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:22253445
|texte= Phosphatidylinositol 4-kinase IIIβ is required for severe acute respiratory syndrome coronavirus spike-mediated cell entry.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:22253445" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |