Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.
Identifieur interne : 001257 ( PubMed/Corpus ); précédent : 001256; suivant : 001258Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.
Auteurs : Amy C. Sims ; Susan C. Tilton ; Vineet D. Menachery ; Lisa E. Gralinski ; Alexandra Sch Fer ; Melissa M. Matzke ; Bobbie-Jo M. Webb-Robertson ; Jean Chang ; Maria L. Luna ; Casey E. Long ; Anil K. Shukla ; Armand R. Bankhead ; Susan E. Burkett ; Gregory Zornetzer ; Chien-Te Kent Tseng ; Thomas O. Metz ; Raymond Pickles ; Shannon Mcweeney ; Richard D. Smith ; Michael G. Katze ; Katrina M. Waters ; Ralph S. BaricSource :
- Journal of virology [ 1098-5514 ] ; 2013.
English descriptors
- KwdEn :
- Active Transport, Cell Nucleus (physiology), Chromatin Immunoprecipitation, Computational Biology (methods), DNA Primers (genetics), Epithelial Cells (metabolism), Epithelial Cells (virology), Gene Regulatory Networks (physiology), Humans, Lung (cytology), Microarray Analysis, Proteomics, Real-Time Polymerase Chain Reaction, SARS Virus (metabolism), Signal Transduction (physiology), Systems Biology (methods), Transcription, Genetic (physiology), Viral Regulatory and Accessory Proteins (metabolism).
- MESH :
- chemical , genetics : DNA Primers.
- cytology : Lung.
- metabolism : Epithelial Cells, SARS Virus, Viral Regulatory and Accessory Proteins.
- methods : Computational Biology, Systems Biology.
- physiology : Active Transport, Cell Nucleus, Gene Regulatory Networks, Signal Transduction, Transcription, Genetic.
- virology : Epithelial Cells.
- Chromatin Immunoprecipitation, Humans, Microarray Analysis, Proteomics, Real-Time Polymerase Chain Reaction.
Abstract
The severe acute respiratory syndrome coronavirus accessory protein ORF6 antagonizes interferon signaling by blocking karyopherin-mediated nuclear import processes. Viral nuclear import antagonists, expressed by several highly pathogenic RNA viruses, likely mediate pleiotropic effects on host gene expression, presumably interfering with transcription factors, cytokines, hormones, and/or signaling cascades that occur in response to infection. By bioinformatic and systems biology approaches, we evaluated the impact of nuclear import antagonism on host expression networks by using human lung epithelial cells infected with either wild-type virus or a mutant that does not express ORF6 protein. Microarray analysis revealed significant changes in differential gene expression, with approximately twice as many upregulated genes in the mutant virus samples by 48 h postinfection, despite identical viral titers. Our data demonstrated that ORF6 protein expression attenuates the activity of numerous karyopherin-dependent host transcription factors (VDR, CREB1, SMAD4, p53, EpasI, and Oct3/4) that are critical for establishing antiviral responses and regulating key host responses during virus infection. Results were confirmed by proteomic and chromatin immunoprecipitation assay analyses and in parallel microarray studies using infected primary human airway epithelial cell cultures. The data strongly support the hypothesis that viral antagonists of nuclear import actively manipulate host responses in specific hierarchical patterns, contributing to the viral pathogenic potential in vivo. Importantly, these studies and modeling approaches not only provide templates for evaluating virus antagonism of nuclear import processes but also can reveal candidate cellular genes and pathways that may significantly influence disease outcomes following severe acute respiratory syndrome coronavirus infection in vivo.
DOI: 10.1128/JVI.02520-12
PubMed: 23365422
Links to Exploration step
pubmed:23365422Le document en format XML
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Gralinski</name></author><author><name sortKey="Sch Fer, Alexandra" sort="Sch Fer, Alexandra" uniqKey="Sch Fer A" first="Alexandra" last="Sch Fer">Alexandra Sch Fer</name></author><author><name sortKey="Matzke, Melissa M" sort="Matzke, Melissa M" uniqKey="Matzke M" first="Melissa M" last="Matzke">Melissa M. Matzke</name></author><author><name sortKey="Webb Robertson, Bobbie Jo M" sort="Webb Robertson, Bobbie Jo M" uniqKey="Webb Robertson B" first="Bobbie-Jo M" last="Webb-Robertson">Bobbie-Jo M. Webb-Robertson</name></author><author><name sortKey="Chang, Jean" sort="Chang, Jean" uniqKey="Chang J" first="Jean" last="Chang">Jean Chang</name></author><author><name sortKey="Luna, Maria L" sort="Luna, Maria L" uniqKey="Luna M" first="Maria L" last="Luna">Maria L. Luna</name></author><author><name sortKey="Long, Casey E" sort="Long, Casey E" uniqKey="Long C" first="Casey E" last="Long">Casey E. Long</name></author><author><name sortKey="Shukla, Anil K" sort="Shukla, Anil K" uniqKey="Shukla A" first="Anil K" last="Shukla">Anil K. Shukla</name></author><author><name sortKey="Bankhead, Armand R" sort="Bankhead, Armand R" uniqKey="Bankhead A" first="Armand R" last="Bankhead">Armand R. Bankhead</name></author><author><name sortKey="Burkett, Susan E" sort="Burkett, Susan E" uniqKey="Burkett S" first="Susan E" last="Burkett">Susan E. Burkett</name></author><author><name sortKey="Zornetzer, Gregory" sort="Zornetzer, Gregory" uniqKey="Zornetzer G" first="Gregory" last="Zornetzer">Gregory Zornetzer</name></author><author><name sortKey="Tseng, Chien Te Kent" sort="Tseng, Chien Te Kent" uniqKey="Tseng C" first="Chien-Te Kent" last="Tseng">Chien-Te Kent Tseng</name></author><author><name sortKey="Metz, Thomas O" sort="Metz, Thomas O" uniqKey="Metz T" first="Thomas O" last="Metz">Thomas O. 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Sims</name><affiliation><nlm:affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. sims0018@ad.unc.edu</nlm:affiliation></affiliation></author><author><name sortKey="Tilton, Susan C" sort="Tilton, Susan C" uniqKey="Tilton S" first="Susan C" last="Tilton">Susan C. Tilton</name></author><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name></author><author><name sortKey="Gralinski, Lisa E" sort="Gralinski, Lisa E" uniqKey="Gralinski L" first="Lisa E" last="Gralinski">Lisa E. Gralinski</name></author><author><name sortKey="Sch Fer, Alexandra" sort="Sch Fer, Alexandra" uniqKey="Sch Fer A" first="Alexandra" last="Sch Fer">Alexandra Sch Fer</name></author><author><name sortKey="Matzke, Melissa M" sort="Matzke, Melissa M" uniqKey="Matzke M" first="Melissa M" last="Matzke">Melissa M. Matzke</name></author><author><name sortKey="Webb Robertson, Bobbie Jo M" sort="Webb Robertson, Bobbie Jo M" uniqKey="Webb Robertson B" first="Bobbie-Jo M" last="Webb-Robertson">Bobbie-Jo M. Webb-Robertson</name></author><author><name sortKey="Chang, Jean" sort="Chang, Jean" uniqKey="Chang J" first="Jean" last="Chang">Jean Chang</name></author><author><name sortKey="Luna, Maria L" sort="Luna, Maria L" uniqKey="Luna M" first="Maria L" last="Luna">Maria L. Luna</name></author><author><name sortKey="Long, Casey E" sort="Long, Casey E" uniqKey="Long C" first="Casey E" last="Long">Casey E. Long</name></author><author><name sortKey="Shukla, Anil K" sort="Shukla, Anil K" uniqKey="Shukla A" first="Anil K" last="Shukla">Anil K. Shukla</name></author><author><name sortKey="Bankhead, Armand R" sort="Bankhead, Armand R" uniqKey="Bankhead A" first="Armand R" last="Bankhead">Armand R. Bankhead</name></author><author><name sortKey="Burkett, Susan E" sort="Burkett, Susan E" uniqKey="Burkett S" first="Susan E" last="Burkett">Susan E. Burkett</name></author><author><name sortKey="Zornetzer, Gregory" sort="Zornetzer, Gregory" uniqKey="Zornetzer G" first="Gregory" last="Zornetzer">Gregory Zornetzer</name></author><author><name sortKey="Tseng, Chien Te Kent" sort="Tseng, Chien Te Kent" uniqKey="Tseng C" first="Chien-Te Kent" last="Tseng">Chien-Te Kent Tseng</name></author><author><name sortKey="Metz, Thomas O" sort="Metz, Thomas O" uniqKey="Metz T" first="Thomas O" last="Metz">Thomas O. Metz</name></author><author><name sortKey="Pickles, Raymond" sort="Pickles, Raymond" uniqKey="Pickles R" first="Raymond" last="Pickles">Raymond Pickles</name></author><author><name sortKey="Mcweeney, Shannon" sort="Mcweeney, Shannon" uniqKey="Mcweeney S" first="Shannon" last="Mcweeney">Shannon Mcweeney</name></author><author><name sortKey="Smith, Richard D" sort="Smith, Richard D" uniqKey="Smith R" first="Richard D" last="Smith">Richard D. Smith</name></author><author><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G" last="Katze">Michael G. Katze</name></author><author><name sortKey="Waters, Katrina M" sort="Waters, Katrina M" uniqKey="Waters K" first="Katrina M" last="Waters">Katrina M. Waters</name></author><author><name sortKey="Baric, Ralph S" sort="Baric, Ralph S" uniqKey="Baric R" first="Ralph S" last="Baric">Ralph S. Baric</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Active Transport, Cell Nucleus (physiology)</term><term>Chromatin Immunoprecipitation</term><term>Computational Biology (methods)</term><term>DNA Primers (genetics)</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (virology)</term><term>Gene Regulatory Networks (physiology)</term><term>Humans</term><term>Lung (cytology)</term><term>Microarray Analysis</term><term>Proteomics</term><term>Real-Time Polymerase Chain Reaction</term><term>SARS Virus (metabolism)</term><term>Signal Transduction (physiology)</term><term>Systems Biology (methods)</term><term>Transcription, Genetic (physiology)</term><term>Viral Regulatory and Accessory Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>SARS Virus</term><term>Viral Regulatory and Accessory Proteins</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Computational Biology</term><term>Systems Biology</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Active Transport, Cell Nucleus</term><term>Gene Regulatory Networks</term><term>Signal Transduction</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatin Immunoprecipitation</term><term>Humans</term><term>Microarray Analysis</term><term>Proteomics</term><term>Real-Time Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus accessory protein ORF6 antagonizes interferon signaling by blocking karyopherin-mediated nuclear import processes. Viral nuclear import antagonists, expressed by several highly pathogenic RNA viruses, likely mediate pleiotropic effects on host gene expression, presumably interfering with transcription factors, cytokines, hormones, and/or signaling cascades that occur in response to infection. By bioinformatic and systems biology approaches, we evaluated the impact of nuclear import antagonism on host expression networks by using human lung epithelial cells infected with either wild-type virus or a mutant that does not express ORF6 protein. Microarray analysis revealed significant changes in differential gene expression, with approximately twice as many upregulated genes in the mutant virus samples by 48 h postinfection, despite identical viral titers. Our data demonstrated that ORF6 protein expression attenuates the activity of numerous karyopherin-dependent host transcription factors (VDR, CREB1, SMAD4, p53, EpasI, and Oct3/4) that are critical for establishing antiviral responses and regulating key host responses during virus infection. Results were confirmed by proteomic and chromatin immunoprecipitation assay analyses and in parallel microarray studies using infected primary human airway epithelial cell cultures. The data strongly support the hypothesis that viral antagonists of nuclear import actively manipulate host responses in specific hierarchical patterns, contributing to the viral pathogenic potential in vivo. Importantly, these studies and modeling approaches not only provide templates for evaluating virus antagonism of nuclear import processes but also can reveal candidate cellular genes and pathways that may significantly influence disease outcomes following severe acute respiratory syndrome coronavirus infection in vivo.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23365422</PMID><DateCompleted><Year>2013</Year><Month>05</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5514</ISSN><JournalIssue CitedMedium="Internet"><Volume>87</Volume><Issue>7</Issue><PubDate><Year>2013</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells.</ArticleTitle><Pagination><MedlinePgn>3885-902</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JVI.02520-12</ELocationID><Abstract><AbstractText>The severe acute respiratory syndrome coronavirus accessory protein ORF6 antagonizes interferon signaling by blocking karyopherin-mediated nuclear import processes. Viral nuclear import antagonists, expressed by several highly pathogenic RNA viruses, likely mediate pleiotropic effects on host gene expression, presumably interfering with transcription factors, cytokines, hormones, and/or signaling cascades that occur in response to infection. By bioinformatic and systems biology approaches, we evaluated the impact of nuclear import antagonism on host expression networks by using human lung epithelial cells infected with either wild-type virus or a mutant that does not express ORF6 protein. Microarray analysis revealed significant changes in differential gene expression, with approximately twice as many upregulated genes in the mutant virus samples by 48 h postinfection, despite identical viral titers. Our data demonstrated that ORF6 protein expression attenuates the activity of numerous karyopherin-dependent host transcription factors (VDR, CREB1, SMAD4, p53, EpasI, and Oct3/4) that are critical for establishing antiviral responses and regulating key host responses during virus infection. Results were confirmed by proteomic and chromatin immunoprecipitation assay analyses and in parallel microarray studies using infected primary human airway epithelial cell cultures. The data strongly support the hypothesis that viral antagonists of nuclear import actively manipulate host responses in specific hierarchical patterns, contributing to the viral pathogenic potential in vivo. Importantly, these studies and modeling approaches not only provide templates for evaluating virus antagonism of nuclear import processes but also can reveal candidate cellular genes and pathways that may significantly influence disease outcomes following severe acute respiratory syndrome coronavirus infection in vivo.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sims</LastName><ForeName>Amy C</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. sims0018@ad.unc.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tilton</LastName><ForeName>Susan C</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Menachery</LastName><ForeName>Vineet D</ForeName><Initials>VD</Initials></Author><Author ValidYN="Y"><LastName>Gralinski</LastName><ForeName>Lisa E</ForeName><Initials>LE</Initials></Author><Author ValidYN="Y"><LastName>Schäfer</LastName><ForeName>Alexandra</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Matzke</LastName><ForeName>Melissa M</ForeName><Initials>MM</Initials></Author><Author ValidYN="Y"><LastName>Webb-Robertson</LastName><ForeName>Bobbie-Jo M</ForeName><Initials>BJ</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Jean</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Luna</LastName><ForeName>Maria L</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Long</LastName><ForeName>Casey E</ForeName><Initials>CE</Initials></Author><Author ValidYN="Y"><LastName>Shukla</LastName><ForeName>Anil K</ForeName><Initials>AK</Initials></Author><Author ValidYN="Y"><LastName>Bankhead</LastName><ForeName>Armand R</ForeName><Initials>AR</Initials><Suffix>3rd</Suffix></Author><Author ValidYN="Y"><LastName>Burkett</LastName><ForeName>Susan E</ForeName><Initials>SE</Initials></Author><Author ValidYN="Y"><LastName>Zornetzer</LastName><ForeName>Gregory</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Tseng</LastName><ForeName>Chien-Te Kent</ForeName><Initials>CT</Initials></Author><Author ValidYN="Y"><LastName>Metz</LastName><ForeName>Thomas O</ForeName><Initials>TO</Initials></Author><Author ValidYN="Y"><LastName>Pickles</LastName><ForeName>Raymond</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>McWeeney</LastName><ForeName>Shannon</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Smith</LastName><ForeName>Richard D</ForeName><Initials>RD</Initials></Author><Author ValidYN="Y"><LastName>Katze</LastName><ForeName>Michael G</ForeName><Initials>MG</Initials></Author><Author ValidYN="Y"><LastName>Waters</LastName><ForeName>Katrina M</ForeName><Initials>KM</Initials></Author><Author ValidYN="Y"><LastName>Baric</LastName><ForeName>Ralph S</ForeName><Initials>RS</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>HHSN272200800060C</GrantID><Acronym>AI</Acronym><Agency>NIAID NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P30 CA069533</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>UL1 TR000128</GrantID><Acronym>TR</Acronym><Agency>NCATS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>01</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Virol</MedlineTA><NlmUniqueID>0113724</NlmUniqueID><ISSNLinking>0022-538X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054334">Viral Regulatory and Accessory Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D021581" MajorTopicYN="N">Active Transport, Cell Nucleus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D047369" MajorTopicYN="N">Chromatin Immunoprecipitation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004847" MajorTopicYN="N">Epithelial Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053263" MajorTopicYN="N">Gene Regulatory Networks</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046228" MajorTopicYN="N">Microarray Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049490" MajorTopicYN="N">Systems Biology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054334" MajorTopicYN="N">Viral Regulatory and Accessory 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IdType="pubmed">19694547</ArticleId></ArticleIdList></Reference><Reference><Citation>J Interferon Cytokine Res. 2009 Sep;29(9):489-98</Citation><ArticleIdList><ArticleId IdType="pubmed">19708811</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2010;5(1):e8729</Citation><ArticleIdList><ArticleId IdType="pubmed">20090954</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010 Feb;6(2):e1000756</Citation><ArticleIdList><ArticleId IdType="pubmed">20140198</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Apr;84(7):3542-51</Citation><ArticleIdList><ArticleId IdType="pubmed">20106914</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010 Apr;6(4):e1000849</Citation><ArticleIdList><ArticleId IdType="pubmed">20386712</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2010 Jul;12(7):873-80</Citation><ArticleIdList><ArticleId IdType="pubmed">20482552</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Aug;84(15):7613-24</Citation><ArticleIdList><ArticleId IdType="pubmed">20504916</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2010 Sep;38(17):5718-34</Citation><ArticleIdList><ArticleId IdType="pubmed">20460467</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Nov;84(21):11297-309</Citation><ArticleIdList><ArticleId IdType="pubmed">20702617</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Nov;84(21):11255-63</Citation><ArticleIdList><ArticleId IdType="pubmed">20719951</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010;6(10):e1001136</Citation><ArticleIdList><ArticleId IdType="pubmed">20949074</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2010 Nov 5;9(11):5748-56</Citation><ArticleIdList><ArticleId IdType="pubmed">20831241</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2011 Mar 4;406(1):127-33</Citation><ArticleIdList><ArticleId IdType="pubmed">21300024</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(4):e18558</Citation><ArticleIdList><ArticleId IdType="pubmed">21533129</ArticleId></ArticleIdList></Reference><Reference><Citation>Thorax. 2011 Aug;66(8):692-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21653927</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2011 Sep;1813(9):1593-606</Citation><ArticleIdList><ArticleId IdType="pubmed">21029754</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2011 Oct;85(19):9658-66</Citation><ArticleIdList><ArticleId IdType="pubmed">21715481</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Oct 15;27(20):2866-72</Citation><ArticleIdList><ArticleId IdType="pubmed">21852304</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2011 Nov;85(21):10955-67</Citation><ArticleIdList><ArticleId IdType="pubmed">21865398</ArticleId></ArticleIdList></Reference><Reference><Citation>FASEB J. 2011 Nov;25(11):3958-65</Citation><ArticleIdList><ArticleId IdType="pubmed">21840941</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Invest. 2011 Dec;121(12):4921-30</Citation><ArticleIdList><ArticleId IdType="pubmed">22105170</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Syst Biol. 2011;5:190</Citation><ArticleIdList><ArticleId IdType="pubmed">22074594</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2011 Dec;11(24):4736-41</Citation><ArticleIdList><ArticleId IdType="pubmed">22038874</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2012 Aug 16;488(7411):409-13</Citation><ArticleIdList><ArticleId IdType="pubmed">22801502</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 Mar;83(5):2368-73</Citation><ArticleIdList><ArticleId IdType="pubmed">19091867</ArticleId></ArticleIdList></Reference><Reference><Citation>Brief Funct Genomic Proteomic. 2006 Dec;5(4):261-72</Citation><ArticleIdList><ArticleId IdType="pubmed">16772273</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Jan;81(2):548-57</Citation><ArticleIdList><ArticleId IdType="pubmed">17108024</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Feb;81(3):1220-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17108045</ArticleId></ArticleIdList></Reference><Reference><Citation>Antiviral Res. 2007 Mar;73(3):219-27</Citation><ArticleIdList><ArticleId IdType="pubmed">17112601</ArticleId></ArticleIdList></Reference><Reference><Citation>Zhonghua Zhong Liu Za Zhi. 2006 Oct;28(10):741-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17366784</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2007 Jan;3(1):e5</Citation><ArticleIdList><ArticleId IdType="pubmed">17222058</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Physiol Lung Cell Mol Physiol. 2007 Jun;292(6):L1361-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17322281</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Jul;81(14):7410-23</Citation><ArticleIdList><ArticleId IdType="pubmed">17507479</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2007 Aug 1;23(15):2021-3</Citation><ArticleIdList><ArticleId IdType="pubmed">17545182</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Sep;81(18):9812-24</Citation><ArticleIdList><ArticleId IdType="pubmed">17596301</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 2007 Oct;27(19):6933-47</Citation><ArticleIdList><ArticleId IdType="pubmed">17646388</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2007 Oct 25;367(2):428-39</Citation><ArticleIdList><ArticleId IdType="pubmed">17617433</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2007 Oct 29;204(11):2529-36</Citation><ArticleIdList><ArticleId IdType="pubmed">17923501</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2002 Jan 1;30(1):207-10</Citation><ArticleIdList><ArticleId IdType="pubmed">11752295</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2002 Jun 7;294(2):354-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12051719</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nutr. 2003 Feb;133(2):374-80</Citation><ArticleIdList><ArticleId IdType="pubmed">12566470</ArticleId></ArticleIdList></Reference><Reference><Citation>Oncogene. 2003 Feb 20;22(7):1057-69</Citation><ArticleIdList><ArticleId IdType="pubmed">12592392</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2003;4(5):P3</Citation><ArticleIdList><ArticleId IdType="pubmed">12734009</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pathol. 2003 Jul;200(3):282-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12845623</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 Oct 10;302(5643):276-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12958366</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12995-3000</Citation><ArticleIdList><ArticleId IdType="pubmed">14569023</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Res. 2003 Nov;13(11):2498-504</Citation><ArticleIdList><ArticleId IdType="pubmed">14597658</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pathol. 2004 Jun;203(2):622-30</Citation><ArticleIdList><ArticleId IdType="pubmed">15141376</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Med. 2005;107:183-206</Citation><ArticleIdList><ArticleId IdType="pubmed">15492373</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 1990 Dec;10(12):6565-77</Citation><ArticleIdList><ArticleId IdType="pubmed">2247074</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1997 Feb 14;272(7):4310-5</Citation><ArticleIdList><ArticleId IdType="pubmed">9020149</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 1998 Dec;79 ( Pt 12):3079-83</Citation><ArticleIdList><ArticleId IdType="pubmed">9880025</ArticleId></ArticleIdList></Reference><Reference><Citation>Oncogene. 1999 Mar 25;18(12):2163-6</Citation><ArticleIdList><ArticleId IdType="pubmed">10321742</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Physiol. 1999 May;276(5 Pt 1):L776-85</Citation><ArticleIdList><ArticleId IdType="pubmed">10330034</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Feb;79(4):2001-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15681402</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 May;79(10):6078-88</Citation><ArticleIdList><ArticleId IdType="pubmed">15857993</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2005 Apr 20;24(8):1634-43</Citation><ArticleIdList><ArticleId IdType="pubmed">15791205</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Jul;79(14):8802-11</Citation><ArticleIdList><ArticleId IdType="pubmed">15994774</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14040-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16169905</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2005 Oct 28;310(5748):676-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16195424</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Dec;79(23):14909-22</Citation><ArticleIdList><ArticleId IdType="pubmed">16282490</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Dec;79(24):15511-24</Citation><ArticleIdList><ArticleId IdType="pubmed">16306622</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Dec 2;280(48):40152-60</Citation><ArticleIdList><ArticleId IdType="pubmed">16204233</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Immunol. 2005 Dec;5(12):917-27</Citation><ArticleIdList><ArticleId IdType="pubmed">16322745</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Dec 9;280(49):40901-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16207705</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2006 Mar;6(6):1783-90</Citation><ArticleIdList><ArticleId IdType="pubmed">16470653</ArticleId></ArticleIdList></Reference><Reference><Citation>Mass Spectrom Rev. 2006 May-Jun;25(3):450-82</Citation><ArticleIdList><ArticleId IdType="pubmed">16429408</ArticleId></ArticleIdList></Reference><Reference><Citation>J Interferon Cytokine Res. 2006 May;26(5):271-80</Citation><ArticleIdList><ArticleId IdType="pubmed">16689655</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Jun;80(11):5156-67</Citation><ArticleIdList><ArticleId IdType="pubmed">16698996</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2006 Dec;5(12):3345-54</Citation><ArticleIdList><ArticleId IdType="pubmed">17137336</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2008 Jan 1;80(1):294-302</Citation><ArticleIdList><ArticleId IdType="pubmed">18044960</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2008 Jan 10;358(2):162-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18184961</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2008 Mar;1783(3):394-404</Citation><ArticleIdList><ArticleId IdType="pubmed">18187047</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2008 Jul 1;24(13):1556-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18453552</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2008 Aug;7(8):3114-26</Citation><ArticleIdList><ArticleId IdType="pubmed">18570455</ArticleId></ArticleIdList></Reference><Reference><Citation>Cancer Res. 2008 Aug 1;68(15):6281-91</Citation><ArticleIdList><ArticleId IdType="pubmed">18676852</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2008 Aug 4;205(8):1929-38</Citation><ArticleIdList><ArticleId IdType="pubmed">18663127</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Biol. 2008 Sep 16;6(9):e226</Citation><ArticleIdList><ArticleId IdType="pubmed">18798692</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2008 Dec;82(23):11948-57</Citation><ArticleIdList><ArticleId IdType="pubmed">18818320</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Res. 2009 Jan;19(1):36-46</Citation><ArticleIdList><ArticleId IdType="pubmed">19114992</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2009;4(1):44-57</Citation><ArticleIdList><ArticleId IdType="pubmed">19131956</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 Feb;83(4):1941-51</Citation><ArticleIdList><ArticleId IdType="pubmed">19073724</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2009 Mar 20;380(4):838-43</Citation><ArticleIdList><ArticleId IdType="pubmed">19338763</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2009;10:87</Citation><ArticleIdList><ArticleId IdType="pubmed">19292916</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 May;83(10):4942-51</Citation><ArticleIdList><ArticleId IdType="pubmed">19244315</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2009;563:177-96</Citation><ArticleIdList><ArticleId IdType="pubmed">19597786</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2009 Jul;5(7):e1000530</Citation><ArticleIdList><ArticleId IdType="pubmed">19649319</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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