A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene.
Identifieur interne : 001213 ( PubMed/Corpus ); précédent : 001212; suivant : 001214A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene.
Auteurs : Qiong Zhao ; Jiayi Zhu ; Weijuan Zhu ; Xunliang Li ; Ye Tao ; Xiaonan Lv ; Xue Wang ; Jiechao Yin ; Cheng He ; Xiaofeng RenSource :
- Monoclonal antibodies in immunodiagnosis and immunotherapy [ 2167-9436 ] ; 2013.
English descriptors
- KwdEn :
- Analysis of Variance, Animals, Antibodies, Monoclonal (immunology), Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect, Hybridomas (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Plasmids (genetics), Spike Glycoprotein, Coronavirus, Transmissible gastroenteritis virus (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Plasmids.
- immunology : Hybridomas, Transmissible gastroenteritis virus.
- Analysis of Variance, Animals, Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus.
Abstract
Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was identified by ELISA. Immunofluorescence assays showed that MAb 1H4 was able to detect infection of cells with TGEV. The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner.
DOI: 10.1089/mab.2012.0067
PubMed: 23600507
Links to Exploration step
pubmed:23600507Le document en format XML
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Xiaonan" uniqKey="Lv X" first="Xiaonan" last="Lv">Xiaonan Lv</name></author><author><name sortKey="Wang, Xue" sort="Wang, Xue" uniqKey="Wang X" first="Xue" last="Wang">Xue Wang</name></author><author><name sortKey="Yin, Jiechao" sort="Yin, Jiechao" uniqKey="Yin J" first="Jiechao" last="Yin">Jiechao Yin</name></author><author><name sortKey="He, Cheng" sort="He, Cheng" uniqKey="He C" first="Cheng" last="He">Cheng He</name></author><author><name sortKey="Ren, Xiaofeng" sort="Ren, Xiaofeng" uniqKey="Ren X" first="Xiaofeng" last="Ren">Xiaofeng Ren</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23600507</idno><idno type="pmid">23600507</idno><idno type="doi">10.1089/mab.2012.0067</idno><idno type="wicri:Area/PubMed/Corpus">001213</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001213</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene.</title><author><name sortKey="Zhao, Qiong" sort="Zhao, Qiong" uniqKey="Zhao Q" first="Qiong" last="Zhao">Qiong Zhao</name><affiliation><nlm:affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Jiayi" sort="Zhu, Jiayi" uniqKey="Zhu J" first="Jiayi" last="Zhu">Jiayi Zhu</name></author><author><name sortKey="Zhu, Weijuan" sort="Zhu, Weijuan" uniqKey="Zhu W" first="Weijuan" last="Zhu">Weijuan Zhu</name></author><author><name sortKey="Li, Xunliang" sort="Li, Xunliang" uniqKey="Li X" first="Xunliang" last="Li">Xunliang Li</name></author><author><name sortKey="Tao, Ye" sort="Tao, Ye" uniqKey="Tao Y" first="Ye" last="Tao">Ye Tao</name></author><author><name sortKey="Lv, Xiaonan" sort="Lv, Xiaonan" uniqKey="Lv X" first="Xiaonan" last="Lv">Xiaonan Lv</name></author><author><name sortKey="Wang, Xue" sort="Wang, Xue" uniqKey="Wang X" first="Xue" last="Wang">Xue Wang</name></author><author><name sortKey="Yin, Jiechao" sort="Yin, Jiechao" uniqKey="Yin J" first="Jiechao" last="Yin">Jiechao Yin</name></author><author><name sortKey="He, Cheng" sort="He, Cheng" uniqKey="He C" first="Cheng" last="He">Cheng He</name></author><author><name sortKey="Ren, Xiaofeng" sort="Ren, Xiaofeng" uniqKey="Ren X" first="Xiaofeng" last="Ren">Xiaofeng Ren</name></author></analytic><series><title level="j">Monoclonal antibodies in immunodiagnosis and immunotherapy</title><idno type="eISSN">2167-9436</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibody Specificity</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique, Indirect</term><term>Hybridomas (immunology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Plasmids (genetics)</term><term>Spike Glycoprotein, Coronavirus</term><term>Transmissible gastroenteritis virus (immunology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hybridomas</term><term>Transmissible gastroenteritis virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Antibody Specificity</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique, Indirect</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Spike Glycoprotein, Coronavirus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was identified by ELISA. Immunofluorescence assays showed that MAb 1H4 was able to detect infection of cells with TGEV. The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23600507</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2167-9436</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Monoclonal antibodies in immunodiagnosis and immunotherapy</Title><ISOAbbreviation>Monoclon Antib Immunodiagn Immunother</ISOAbbreviation></Journal><ArticleTitle>A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene.</ArticleTitle><Pagination><MedlinePgn>50-4</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1089/mab.2012.0067</ELocationID><Abstract><AbstractText>Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was identified by ELISA. Immunofluorescence assays showed that MAb 1H4 was able to detect infection of cells with TGEV. The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Qiong</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Jiayi</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Weijuan</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xunliang</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Ye</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Xiaonan</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xue</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Jiechao</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Cheng</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Xiaofeng</ForeName><Initials>X</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Monoclon Antib Immunodiagn Immunother</MedlineTA><NlmUniqueID>101590955</NlmUniqueID><ISSNLinking>2167-9436</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C578553">MHV surface projection 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