SrasV1, PubMed, Corpus, bibRecord, 000F56

Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes.

Identifieur interne : 000F56 ( PubMed/Corpus ); précédent : 000F55; suivant : 000F57

Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes.

Auteurs : Mickaël Bouvet ; Adrien Lugari ; Clara C. Posthuma ; Jessika C. Zevenhoven ; Stéphanie Bernard ; Stéphane Betzi ; Isabelle Imbert ; Bruno Canard ; Jean-Claude Guillemot ; Patrick Lécine ; Susanne Pfefferle ; Christian Drosten ; Eric J. Snijder ; Etienne Decroly ; Xavier Morelli

Source :

The Journal of biological chemistry [ 1083-351X ] ; 2014.

RBID : pubmed:25074927

English descriptors

KwdEn :
- Archaeal Proteins (genetics), Archaeal Proteins (metabolism), Coronavirus Infections (enzymology), Coronavirus Infections (pathology), Crystallography, X-Ray, Exoribonucleases (genetics), Exoribonucleases (metabolism), Humans, Methyltransferases (genetics), Methyltransferases (metabolism), Mutagenesis, Protein Interaction Maps (genetics), SARS Virus (enzymology), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Virus Replication (genetics).
MESH :
- chemical , genetics : Archaeal Proteins, Exoribonucleases, Methyltransferases, Viral Nonstructural Proteins.
- chemical , metabolism : Archaeal Proteins, Exoribonucleases, Methyltransferases, Viral Nonstructural Proteins.
- enzymology : Coronavirus Infections, SARS Virus.
- genetics : Protein Interaction Maps, Virus Replication.
- pathology : Coronavirus Infections.
- Crystallography, X-Ray, Humans, Mutagenesis.

Abstract

The RNA-synthesizing machinery of the severe acute respiratory syndrome Coronavirus (SARS-CoV) is composed of 16 non-structural proteins (nsp1-16) encoded by ORF1a/1b. The 148-amino acid nsp10 subunit contains two zinc fingers and is known to interact with both nsp14 and nsp16, stimulating their respective 3'-5' exoribonuclease and 2'-O-methyltransferase activities. Using alanine-scanning mutagenesis, in cellulo bioluminescence resonance energy transfer experiments, and in vitro pulldown assays, we have now identified the key residues on the nsp10 surface that interact with nsp14. The functional consequences of mutations introduced at these positions were first evaluated biochemically by monitoring nsp14 exoribonuclease activity. Disruption of the nsp10-nsp14 interaction abrogated the nsp10-driven activation of the nsp14 exoribonuclease. We further showed that the nsp10 surface interacting with nsp14 overlaps with the surface involved in the nsp10-mediated activation of nsp16 2'-O-methyltransferase activity, suggesting that nsp10 is a major regulator of SARS-CoV replicase function. In line with this notion, reverse genetics experiments supported an essential role of the nsp10 surface that interacts with nsp14 in SARS-CoV replication, as several mutations that abolished the interaction in vitro yielded a replication-negative viral phenotype. In contrast, mutants in which the nsp10-nsp16 interaction was disturbed proved to be crippled but viable. These experiments imply that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses.

DOI: 10.1074/jbc.M114.577353
PubMed: 25074927

Links to Exploration step

pubmed:25074927

Le document en format XML

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<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
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<author><name sortKey="Guillemot, Jean Claude" sort="Guillemot, Jean Claude" uniqKey="Guillemot J" first="Jean-Claude" last="Guillemot">Jean-Claude Guillemot</name>
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<author><name sortKey="Lecine, Patrick" sort="Lecine, Patrick" uniqKey="Lecine P" first="Patrick" last="Lécine">Patrick Lécine</name>
<affiliation><nlm:affiliation>CIRI, INSERM U1111, CNRS UMR5308, Université Lyon 1, ENS de Lyon, 69007 Lyon, France, and.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Pfefferle, Susanne" sort="Pfefferle, Susanne" uniqKey="Pfefferle S" first="Susanne" last="Pfefferle">Susanne Pfefferle</name>
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<author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name>
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<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
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<term>Archaeal Proteins (metabolism)</term>
<term>Coronavirus Infections (enzymology)</term>
<term>Coronavirus Infections (pathology)</term>
<term>Crystallography, X-Ray</term>
<term>Exoribonucleases (genetics)</term>
<term>Exoribonucleases (metabolism)</term>
<term>Humans</term>
<term>Methyltransferases (genetics)</term>
<term>Methyltransferases (metabolism)</term>
<term>Mutagenesis</term>
<term>Protein Interaction Maps (genetics)</term>
<term>SARS Virus (enzymology)</term>
<term>Viral Nonstructural Proteins (genetics)</term>
<term>Viral Nonstructural Proteins (metabolism)</term>
<term>Virus Replication (genetics)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Archaeal Proteins</term>
<term>Exoribonucleases</term>
<term>Methyltransferases</term>
<term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Archaeal Proteins</term>
<term>Exoribonucleases</term>
<term>Methyltransferases</term>
<term>Viral Nonstructural Proteins</term>
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<term>SARS Virus</term>
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<term>Virus Replication</term>
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<front><div type="abstract" xml:lang="en">The RNA-synthesizing machinery of the severe acute respiratory syndrome Coronavirus (SARS-CoV) is composed of 16 non-structural proteins (nsp1-16) encoded by ORF1a/1b. The 148-amino acid nsp10 subunit contains two zinc fingers and is known to interact with both nsp14 and nsp16, stimulating their respective 3'-5' exoribonuclease and 2'-O-methyltransferase activities. Using alanine-scanning mutagenesis, in cellulo bioluminescence resonance energy transfer experiments, and in vitro pulldown assays, we have now identified the key residues on the nsp10 surface that interact with nsp14. The functional consequences of mutations introduced at these positions were first evaluated biochemically by monitoring nsp14 exoribonuclease activity. Disruption of the nsp10-nsp14 interaction abrogated the nsp10-driven activation of the nsp14 exoribonuclease. We further showed that the nsp10 surface interacting with nsp14 overlaps with the surface involved in the nsp10-mediated activation of nsp16 2'-O-methyltransferase activity, suggesting that nsp10 is a major regulator of SARS-CoV replicase function. In line with this notion, reverse genetics experiments supported an essential role of the nsp10 surface that interacts with nsp14 in SARS-CoV replication, as several mutations that abolished the interaction in vitro yielded a replication-negative viral phenotype. In contrast, mutants in which the nsp10-nsp16 interaction was disturbed proved to be crippled but viable. These experiments imply that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses. </div>
</front>
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<DateCompleted><Year>2015</Year>
<Month>02</Month>
<Day>19</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>01</Day>
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<Issue>37</Issue>
<PubDate><Year>2014</Year>
<Month>Sep</Month>
<Day>12</Day>
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<Title>The Journal of biological chemistry</Title>
<ISOAbbreviation>J. Biol. Chem.</ISOAbbreviation>
</Journal>
<ArticleTitle>Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes.</ArticleTitle>
<Pagination><MedlinePgn>25783-96</MedlinePgn>
</Pagination>
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<Abstract><AbstractText>The RNA-synthesizing machinery of the severe acute respiratory syndrome Coronavirus (SARS-CoV) is composed of 16 non-structural proteins (nsp1-16) encoded by ORF1a/1b. The 148-amino acid nsp10 subunit contains two zinc fingers and is known to interact with both nsp14 and nsp16, stimulating their respective 3'-5' exoribonuclease and 2'-O-methyltransferase activities. Using alanine-scanning mutagenesis, in cellulo bioluminescence resonance energy transfer experiments, and in vitro pulldown assays, we have now identified the key residues on the nsp10 surface that interact with nsp14. The functional consequences of mutations introduced at these positions were first evaluated biochemically by monitoring nsp14 exoribonuclease activity. Disruption of the nsp10-nsp14 interaction abrogated the nsp10-driven activation of the nsp14 exoribonuclease. We further showed that the nsp10 surface interacting with nsp14 overlaps with the surface involved in the nsp10-mediated activation of nsp16 2'-O-methyltransferase activity, suggesting that nsp10 is a major regulator of SARS-CoV replicase function. In line with this notion, reverse genetics experiments supported an essential role of the nsp10 surface that interacts with nsp14 in SARS-CoV replication, as several mutations that abolished the interaction in vitro yielded a replication-negative viral phenotype. In contrast, mutants in which the nsp10-nsp16 interaction was disturbed proved to be crippled but viable. These experiments imply that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses. </AbstractText>
<CopyrightInformation>© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bouvet</LastName>
<ForeName>Mickaël</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>From the Aix-Marseille Université, AFMB UMR 7257, 13288 Marseille, France, CNRS, AFMB UMR 7257, 13288 Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lugari</LastName>
<ForeName>Adrien</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Cancer Research Center of Marseille (CRCM), CNRS UMR7258, INSERM U1068, Institut Paoli-Calmettes, Aix-Marseille Université, F-13009 Marseille, France.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Posthuma</LastName>
<ForeName>Clara C</ForeName>
<Initials>CC</Initials>
<AffiliationInfo><Affiliation>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, P. O. Box 9600, 2300RC Leiden, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zevenhoven</LastName>
<ForeName>Jessika C</ForeName>
<Initials>JC</Initials>
<AffiliationInfo><Affiliation>Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, P. O. Box 9600, 2300RC Leiden, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bernard</LastName>
<ForeName>Stéphanie</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Cancer Research Center of Marseille (CRCM), CNRS UMR7258, INSERM U1068, Institut Paoli-Calmettes, Aix-Marseille Université, F-13009 Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Betzi</LastName>
<ForeName>Stéphane</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Cancer Research Center of Marseille (CRCM), CNRS UMR7258, INSERM U1068, Institut Paoli-Calmettes, Aix-Marseille Université, F-13009 Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Imbert</LastName>
<ForeName>Isabelle</ForeName>
<Initials>I</Initials>
<AffiliationInfo><Affiliation>From the Aix-Marseille Université, AFMB UMR 7257, 13288 Marseille, France, CNRS, AFMB UMR 7257, 13288 Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Canard</LastName>
<ForeName>Bruno</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>From the Aix-Marseille Université, AFMB UMR 7257, 13288 Marseille, France, CNRS, AFMB UMR 7257, 13288 Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Guillemot</LastName>
<ForeName>Jean-Claude</ForeName>
<Initials>JC</Initials>
<AffiliationInfo><Affiliation>From the Aix-Marseille Université, AFMB UMR 7257, 13288 Marseille, France, CNRS, AFMB UMR 7257, 13288 Marseille, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lécine</LastName>
<ForeName>Patrick</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>CIRI, INSERM U1111, CNRS UMR5308, Université Lyon 1, ENS de Lyon, 69007 Lyon, France, and.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pfefferle</LastName>
<ForeName>Susanne</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Institute of Virology, University of Bonn Medical Center, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Drosten</LastName>
<ForeName>Christian</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Institute of Virology, University of Bonn Medical Center, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Snijder</LastName>
<ForeName>Eric J</ForeName>
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<ArticleIdList><ArticleId IdType="pubmed">25074927</ArticleId>
<ArticleId IdType="pii">M114.577353</ArticleId>
<ArticleId IdType="doi">10.1074/jbc.M114.577353</ArticleId>
<ArticleId IdType="pmc">PMC4162180</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 2004 Jul;78(14):7833-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15220459</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Biol. 2008 Sep 16;6(9):e226</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18798692</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Rev Med Virol. 2010 Jan;20(1):4-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20041442</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cold Spring Harb Symp Quant Biol. 2001;66:301-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12762032</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2014 Jan;88(1):717-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24131722</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 May;79(9):5288-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15827143</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 2002 Dec 2;21(23):6571-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12456663</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2008 May 02;4(5):e1000054</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18451981</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Med. 2004 Dec;10(12 Suppl):S88-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15577937</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biotechnol J. 2008 Mar;3(3):311-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18228541</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2005 Dec;1(4):e39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16341254</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Rev Microbiol. 2003 Dec;1(3):209-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15035025</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):404-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21393853</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>EMBO J. 1987 Dec 1;6(12):3779-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3428275</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Jul;87(14):7790-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23678167</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2007 Jul;81(13):7086-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17428870</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virus Res. 2008 May;133(2):136-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18255185</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS One. 2008 Oct 01;3(10):e3299</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18827877</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Prog Nucleic Acid Res Mol Biol. 2001;66:1-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11051760</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Methods Mol Biol. 2010;634:421-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20677001</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Rev Mol Cell Biol. 2002 Aug;3(8):619-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12154373</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2010 May 06;6(5):e1000896</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20463816</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Mol Biol. 2003 Aug 29;331(5):991-1004</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12927536</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):151-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9874787</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1981 May;38(2):661-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6165837</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2012 Nov 8;367(19):1814-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>RNA Biol. 2011 Mar-Apr;8(2):270-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21593585</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Curr Opin Struct Biol. 2005 Feb;15(1):99-106</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15718140</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Immunol. 2011 Feb;12(2):137-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21217758</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2006 Aug;80(16):7902-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16873247</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell. 1989 May 19;57(4):537-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2720781</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Bioinformatics. 1999 Apr;15(4):305-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10320398</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2010 Nov 18;468(7322):452-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21085181</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2010 Oct 22;285(43):33230-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20699222</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2007 Jun;81(12):6356-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17392363</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2007 Nov;81(22):12135-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17804504</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5108-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16549795</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2014 Apr;88(8):4251-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24478444</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1982 Feb;41(2):557-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6281467</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Adv Virus Res. 2006;66:193-292</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16877062</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 May;87(10):5812-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23487465</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virus Res. 2006 Apr;117(1):17-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16503362</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Viruses. 2013 Jan 18;5(1):279-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23334702</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Antiviral Res. 2014 Jan;101:122-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24269475</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2006 Aug;80(16):7894-901</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16873246</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>mBio. 2012 Nov 20;3(6):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23170002</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Gen Virol. 2006 Jun;87(Pt 6):1403-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16690906</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Euro Surveill. 2012 Oct 04;17(40):20290</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23078800</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3484-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19208801</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2010 Apr 22;6(4):e1000863</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20421945</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol J. 2009 Aug 24;6:131</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19698190</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Rev Microbiol. 2011 Dec 05;10(1):51-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22138959</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2011 May;7(5):e1002059</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21637813</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2013 Aug;9(8):e1003565</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23966862</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell. 2003 Jun 13;113(6):701-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12809601</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virus Res. 2012 Aug;167(2):322-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22659295</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nat Med. 2012 Dec;18(12):1820-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23142821</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2007 Jan;81(1):20-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16928755</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Curr Opin Virol. 2013 Feb;3(1):84-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23265969</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol Sin. 2010 Oct;25(5):361-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20960182</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2006 Jun;80(12):5927-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16731931</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1999 Sep;73(9):7641-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10438855</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>PLoS Pathog. 2011 Sep;7(9):e1002215</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21931546</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Adv Exp Med Biol. 2006;581:153-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17037523</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9372-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22635272</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2013 Jun;87(11):6296-305</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23536667</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes.

Coronavirus Nsp10, a critical co-factor for activation of multiple replicative enzymes.

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