Spatiotemporal interplay of severe acute respiratory syndrome coronavirus and respiratory mucosal cells drives viral dissemination in rhesus macaques.
Identifieur interne : 000D32 ( PubMed/Corpus ); précédent : 000D31; suivant : 000D33Spatiotemporal interplay of severe acute respiratory syndrome coronavirus and respiratory mucosal cells drives viral dissemination in rhesus macaques.
Auteurs : L. Liu ; Q. Wei ; K. Nishiura ; J. Peng ; H. Wang ; C. Midkiff ; X. Alvarez ; C. Qin ; A. Lackner ; Z. ChenSource :
- Mucosal immunology [ 1935-3456 ] ; 2016.
English descriptors
- KwdEn :
- Animals, Cell Differentiation, Cell Movement, Cells, Cultured, Coronavirus (physiology), Dendritic Cells (immunology), Dendritic Cells (virology), Humans, Immune Evasion, Immunity, Innate, Macaca mulatta (immunology), Macrophages (immunology), Macrophages (virology), RNA, Viral (immunology), Respiratory Mucosa (immunology), Respiratory Mucosa (virology), Severe Acute Respiratory Syndrome (immunology), T-Lymphocytes (immunology), T-Lymphocytes (virology), Viral Load, Virus Replication, Virus Shedding.
- MESH :
- chemical , immunology : RNA, Viral.
- immunology : Dendritic Cells, Macaca mulatta, Macrophages, Respiratory Mucosa, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- physiology : Coronavirus.
- virology : Dendritic Cells, Macrophages, Respiratory Mucosa, T-Lymphocytes.
- Animals, Cell Differentiation, Cell Movement, Cells, Cultured, Humans, Immune Evasion, Immunity, Innate, Viral Load, Virus Replication, Virus Shedding.
Abstract
Innate immune responses have a critical role in the control of early virus replication and dissemination. It remains unknown, however, how severe acute respiratory syndrome coronavirus (SARS-CoV) evades respiratory innate immunity to establish a systemic infection. Here we show in Chinese macaques that SARS-CoV traversed the mucosa through the respiratory tract within 2 days, resulting in extensive mucosal infiltration by T cells, MAC387(+), and CD163(+) monocytes/macrophages followed by limited viral replication in the lung but persistent viral shedding into the upper airway. Mucosal monocytes/macrophages sequestered virions in intracellular vesicles together with infected Langerhans cells and migrated into the tonsils and/or draining lymph nodes within 2 days. In lymphoid tissues, viral RNA and proteins were detected in infected monocytes upon differentiation into dendritic cells (DCs) within 3 days. Systemic viral dissemination was observed within 7 days. This study provides a comprehensive overview of the spatiotemporal interactions of SARS-CoV, monocytes/macrophages, and the DC network in mucosal tissues and highlights the fact that, while these innate cells contribute to viral clearance, they probably also serve as shelters and vehicles to provide a mechanism for the virus to escape host mucosal innate immunity and disseminate systemically.
DOI: 10.1038/mi.2015.127
PubMed: 26647718
Links to Exploration step
pubmed:26647718Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Spatiotemporal interplay of severe acute respiratory syndrome coronavirus and respiratory mucosal cells drives viral dissemination in rhesus macaques.</title><author><name sortKey="Liu, L" sort="Liu, L" uniqKey="Liu L" first="L" last="Liu">L. Liu</name><affiliation><nlm:affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Wei, Q" sort="Wei, Q" uniqKey="Wei Q" first="Q" last="Wei">Q. Wei</name><affiliation><nlm:affiliation>Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Nishiura, K" sort="Nishiura, K" uniqKey="Nishiura K" first="K" last="Nishiura">K. Nishiura</name><affiliation><nlm:affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Peng, J" sort="Peng, J" uniqKey="Peng J" first="J" last="Peng">J. Peng</name><affiliation><nlm:affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, H" sort="Wang, H" uniqKey="Wang H" first="H" last="Wang">H. Wang</name><affiliation><nlm:affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Midkiff, C" sort="Midkiff, C" uniqKey="Midkiff C" first="C" last="Midkiff">C. Midkiff</name><affiliation><nlm:affiliation>Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Alvarez, X" sort="Alvarez, X" uniqKey="Alvarez X" first="X" last="Alvarez">X. Alvarez</name><affiliation><nlm:affiliation>Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Qin, C" sort="Qin, C" uniqKey="Qin C" first="C" last="Qin">C. Qin</name><affiliation><nlm:affiliation>Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, P.R. 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China.</nlm:affiliation></affiliation></author><author><name sortKey="Wei, Q" sort="Wei, Q" uniqKey="Wei Q" first="Q" last="Wei">Q. Wei</name><affiliation><nlm:affiliation>Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Nishiura, K" sort="Nishiura, K" uniqKey="Nishiura K" first="K" last="Nishiura">K. Nishiura</name><affiliation><nlm:affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Peng, J" sort="Peng, J" uniqKey="Peng J" first="J" last="Peng">J. Peng</name><affiliation><nlm:affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, H" sort="Wang, H" uniqKey="Wang H" first="H" last="Wang">H. Wang</name><affiliation><nlm:affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Midkiff, C" sort="Midkiff, C" uniqKey="Midkiff C" first="C" last="Midkiff">C. Midkiff</name><affiliation><nlm:affiliation>Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Alvarez, X" sort="Alvarez, X" uniqKey="Alvarez X" first="X" last="Alvarez">X. Alvarez</name><affiliation><nlm:affiliation>Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Qin, C" sort="Qin, C" uniqKey="Qin C" first="C" last="Qin">C. Qin</name><affiliation><nlm:affiliation>Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Lackner, A" sort="Lackner, A" uniqKey="Lackner A" first="A" last="Lackner">A. 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China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mucosal immunology</title><idno type="eISSN">1935-3456</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Differentiation</term><term>Cell Movement</term><term>Cells, Cultured</term><term>Coronavirus (physiology)</term><term>Dendritic Cells (immunology)</term><term>Dendritic Cells (virology)</term><term>Humans</term><term>Immune Evasion</term><term>Immunity, Innate</term><term>Macaca mulatta (immunology)</term><term>Macrophages (immunology)</term><term>Macrophages (virology)</term><term>RNA, Viral (immunology)</term><term>Respiratory Mucosa (immunology)</term><term>Respiratory Mucosa (virology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>T-Lymphocytes (immunology)</term><term>T-Lymphocytes (virology)</term><term>Viral Load</term><term>Virus Replication</term><term>Virus Shedding</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Dendritic Cells</term><term>Macaca mulatta</term><term>Macrophages</term><term>Respiratory Mucosa</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Dendritic Cells</term><term>Macrophages</term><term>Respiratory Mucosa</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Differentiation</term><term>Cell Movement</term><term>Cells, Cultured</term><term>Humans</term><term>Immune Evasion</term><term>Immunity, Innate</term><term>Viral Load</term><term>Virus Replication</term><term>Virus Shedding</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Innate immune responses have a critical role in the control of early virus replication and dissemination. It remains unknown, however, how severe acute respiratory syndrome coronavirus (SARS-CoV) evades respiratory innate immunity to establish a systemic infection. Here we show in Chinese macaques that SARS-CoV traversed the mucosa through the respiratory tract within 2 days, resulting in extensive mucosal infiltration by T cells, MAC387(+), and CD163(+) monocytes/macrophages followed by limited viral replication in the lung but persistent viral shedding into the upper airway. Mucosal monocytes/macrophages sequestered virions in intracellular vesicles together with infected Langerhans cells and migrated into the tonsils and/or draining lymph nodes within 2 days. In lymphoid tissues, viral RNA and proteins were detected in infected monocytes upon differentiation into dendritic cells (DCs) within 3 days. Systemic viral dissemination was observed within 7 days. This study provides a comprehensive overview of the spatiotemporal interactions of SARS-CoV, monocytes/macrophages, and the DC network in mucosal tissues and highlights the fact that, while these innate cells contribute to viral clearance, they probably also serve as shelters and vehicles to provide a mechanism for the virus to escape host mucosal innate immunity and disseminate systemically.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26647718</PMID><DateCompleted><Year>2017</Year><Month>11</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>29</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1935-3456</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>07</Month></PubDate></JournalIssue><Title>Mucosal immunology</Title><ISOAbbreviation>Mucosal Immunol</ISOAbbreviation></Journal><ArticleTitle>Spatiotemporal interplay of severe acute respiratory syndrome coronavirus and respiratory mucosal cells drives viral dissemination in rhesus macaques.</ArticleTitle><Pagination><MedlinePgn>1089-101</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/mi.2015.127</ELocationID><Abstract><AbstractText>Innate immune responses have a critical role in the control of early virus replication and dissemination. It remains unknown, however, how severe acute respiratory syndrome coronavirus (SARS-CoV) evades respiratory innate immunity to establish a systemic infection. Here we show in Chinese macaques that SARS-CoV traversed the mucosa through the respiratory tract within 2 days, resulting in extensive mucosal infiltration by T cells, MAC387(+), and CD163(+) monocytes/macrophages followed by limited viral replication in the lung but persistent viral shedding into the upper airway. Mucosal monocytes/macrophages sequestered virions in intracellular vesicles together with infected Langerhans cells and migrated into the tonsils and/or draining lymph nodes within 2 days. In lymphoid tissues, viral RNA and proteins were detected in infected monocytes upon differentiation into dendritic cells (DCs) within 3 days. Systemic viral dissemination was observed within 7 days. This study provides a comprehensive overview of the spatiotemporal interactions of SARS-CoV, monocytes/macrophages, and the DC network in mucosal tissues and highlights the fact that, while these innate cells contribute to viral clearance, they probably also serve as shelters and vehicles to provide a mechanism for the virus to escape host mucosal innate immunity and disseminate systemically.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Q</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nishiura</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Midkiff</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alvarez</LastName><ForeName>X</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qin</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lackner</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Z</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>AIDS Institute and Research Center for Infection and Immunology, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P51 OD011104</GrantID><Acronym>OD</Acronym><Agency>NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P51 RR000164</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 HL080211</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>S10 OD019964</GrantID><Acronym>OD</Acronym><Agency>NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>12</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mucosal Immunol</MedlineTA><NlmUniqueID>101299742</NlmUniqueID><ISSNLinking>1933-0219</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002465" MajorTopicYN="N">Cell Movement</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName><QualifierName UI="Q000502" 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MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020545" MajorTopicYN="N">Respiratory Mucosa</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019562" MajorTopicYN="N">Viral 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IdType="pubmed">26647718</ArticleId><ArticleId IdType="pii">mi2015127</ArticleId><ArticleId IdType="doi">10.1038/mi.2015.127</ArticleId><ArticleId IdType="pmc">PMC4900951</ArticleId><ArticleId IdType="mid">NIHMS736072</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Blood. 2005 Oct 1;106(7):2366-74</Citation><ArticleIdList><ArticleId IdType="pubmed">15860669</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2005 Sep;11(9):944-51</Citation><ArticleIdList><ArticleId IdType="pubmed">16116432</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Biol. 2007 Apr 23;177(2):329-41</Citation><ArticleIdList><ArticleId IdType="pubmed">17438075</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Med. 2006 Feb;3(2):e27</Citation><ArticleIdList><ArticleId IdType="pubmed">16379499</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2011 Apr;85(8):4025-30</Citation><ArticleIdList><ArticleId IdType="pubmed">21289121</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation><ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2012 Nov 8;367 (19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2008 Nov 10;381(1):89-97</Citation><ArticleIdList><ArticleId IdType="pubmed">18801550</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(7):e39990</Citation><ArticleIdList><ArticleId IdType="pubmed">22808082</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 May 24;361(9371):1767-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12781535</ArticleId></ArticleIdList></Reference><Reference><Citation>Nephrol Dial Transplant. 2004 Dec;19(12):3180-2</Citation><ArticleIdList><ArticleId IdType="pubmed">15575009</ArticleId></ArticleIdList></Reference><Reference><Citation>Cytokine. 2000 Sep;12(9):1312-21</Citation><ArticleIdList><ArticleId IdType="pubmed">10975989</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2000 Mar 3;100(5):575-85</Citation><ArticleIdList><ArticleId IdType="pubmed">10721994</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Virol. 2006 Jul;87(Pt 7):1953-60</Citation><ArticleIdList><ArticleId IdType="pubmed">16760397</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2012 Mar;86(5):2826-36</Citation><ArticleIdList><ArticleId IdType="pubmed">22205742</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect Dis. 2005 Jan 15;191(2):193-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15609228</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunity. 2007 Feb;26(2):257-70</Citation><ArticleIdList><ArticleId IdType="pubmed">17306567</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Host Microbe. 2013 Feb 13;13(2):155-68</Citation><ArticleIdList><ArticleId IdType="pubmed">23414756</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pathol. 2005 Jul;206(3):251-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15892035</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2013 Feb 21;18(8):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23449231</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Mar;80(6):2684-93</Citation><ArticleIdList><ArticleId IdType="pubmed">16501078</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pathol. 2004 Feb;202(2):157-63</Citation><ArticleIdList><ArticleId IdType="pubmed">14743497</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2003 Sep 1;171(5):2262-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12928370</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2007 Mar;3(3):e36</Citation><ArticleIdList><ArticleId IdType="pubmed">17381240</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 1993 Dec;67(12):7340-9</Citation><ArticleIdList><ArticleId IdType="pubmed">7693969</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pathol. 2003 Jul;200(3):282-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12845623</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunity. 2013 Feb 21;38(2):309-21</Citation><ArticleIdList><ArticleId IdType="pubmed">23438822</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Med. 2006 May;3(5):e149</Citation><ArticleIdList><ArticleId IdType="pubmed">16605302</ArticleId></ArticleIdList></Reference><Reference><Citation>Mucosal Immunol. 2009 Mar;2(2):122-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19129759</ArticleId></ArticleIdList></Reference><Reference><Citation>J Leukoc Biol. 2000 Jan;67(1):97-103</Citation><ArticleIdList><ArticleId IdType="pubmed">10648003</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21641-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21098292</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2012 Jan 16;209(1):139-55</Citation><ArticleIdList><ArticleId IdType="pubmed">22231304</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Jun;79(12):7819-26</Citation><ArticleIdList><ArticleId IdType="pubmed">15919935</ArticleId></ArticleIdList></Reference><Reference><Citation>Mucosal Immunol. 2010 May;3(3):280-90</Citation><ArticleIdList><ArticleId IdType="pubmed">20147895</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Med. 2005 Aug 1;202(3):415-24</Citation><ArticleIdList><ArticleId IdType="pubmed">16043521</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2008 Mar 07;4(3):e1000015</Citation><ArticleIdList><ArticleId IdType="pubmed">18369466</ArticleId></ArticleIdList></Reference><Reference><Citation>J Extracell Vesicles. 2013 Sep 12;2:null</Citation><ArticleIdList><ArticleId IdType="pubmed">24223256</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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