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Cytoplasmic tail of coronavirus spike protein has intracellular targeting signals.

Identifieur interne : 000B19 ( PubMed/Corpus ); précédent : 000B18; suivant : 000B20

Cytoplasmic tail of coronavirus spike protein has intracellular targeting signals.

Auteurs : Jibin Sadasivan ; Manmeet Singh ; Jayasri Das Sarma

Source :

Journal of biosciences [ 0973-7138 ] ; 2017.

RBID : pubmed:28569247

English descriptors

KwdEn :
- Amino Acid Motifs, Amino Acid Sequence, Coronavirus (metabolism), Cytoplasm, Gene Expression Regulation, Viral (physiology), HeLa Cells, Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (physiology), Mutation, Protein Conformation, Protein Sorting Signals, Protein Transport, Spike Glycoprotein, Coronavirus (chemistry), Spike Glycoprotein, Coronavirus (metabolism).
MESH :
- chemical , chemistry : Membrane Glycoproteins, Spike Glycoprotein, Coronavirus.
- metabolism : Coronavirus, Spike Glycoprotein, Coronavirus.
- physiology : Gene Expression Regulation, Viral, Membrane Glycoproteins.
- Amino Acid Motifs, Amino Acid Sequence, Cytoplasm, HeLa Cells, Humans, Mutation, Protein Conformation, Protein Sorting Signals, Protein Transport.

Abstract

Intracellular trafficking and localization studies of spike protein from SARS and OC43 showed that SARS spike protein is localized in the ER or ERGIC compartment and OC43 spike protein is predominantly localized in the lysosome. Differential localization can be explained by signal sequence. The sequence alignment using Clustal W shows that the signal sequence present at the cytoplasmic tail plays an important role in spike protein localization. A unique GYQEL motif is identified at the cytoplasmic terminal of OC43 spike protein which helps in localization in the lysosome, and a novel KLHYT motif is identified in the cytoplasmic tail of SARS spike protein which helps in ER or ERGIC localization. This study sheds some light on the role of cytoplasmic tail of spike protein in cell-to-cell fusion, coronavirus host cell fusion and subsequent pathogenicity.

DOI: 10.1007/s12038-017-9676-7
PubMed: 28569247

Links to Exploration step

pubmed:28569247

Le document en format XML

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<author><name sortKey="Sadasivan, Jibin" sort="Sadasivan, Jibin" uniqKey="Sadasivan J" first="Jibin" last="Sadasivan">Jibin Sadasivan</name>
<affiliation><nlm:affiliation>Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur 741 246, India.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Singh, Manmeet" sort="Singh, Manmeet" uniqKey="Singh M" first="Manmeet" last="Singh">Manmeet Singh</name>
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<author><name sortKey="Sarma, Jayasri Das" sort="Sarma, Jayasri Das" uniqKey="Sarma J" first="Jayasri Das" last="Sarma">Jayasri Das Sarma</name>
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<author><name sortKey="Singh, Manmeet" sort="Singh, Manmeet" uniqKey="Singh M" first="Manmeet" last="Singh">Manmeet Singh</name>
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<author><name sortKey="Sarma, Jayasri Das" sort="Sarma, Jayasri Das" uniqKey="Sarma J" first="Jayasri Das" last="Sarma">Jayasri Das Sarma</name>
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<series><title level="j">Journal of biosciences</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Amino Acid Sequence</term>
<term>Coronavirus (metabolism)</term>
<term>Cytoplasm</term>
<term>Gene Expression Regulation, Viral (physiology)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (physiology)</term>
<term>Mutation</term>
<term>Protein Conformation</term>
<term>Protein Sorting Signals</term>
<term>Protein Transport</term>
<term>Spike Glycoprotein, Coronavirus (chemistry)</term>
<term>Spike Glycoprotein, Coronavirus (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Spike Glycoprotein, Coronavirus</term>
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<term>Spike Glycoprotein, Coronavirus</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Viral</term>
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</keywords>
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<term>Cytoplasm</term>
<term>HeLa Cells</term>
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<front><div type="abstract" xml:lang="en">Intracellular trafficking and localization studies of spike protein from SARS and OC43 showed that SARS spike protein is localized in the ER or ERGIC compartment and OC43 spike protein is predominantly localized in the lysosome. Differential localization can be explained by signal sequence. The sequence alignment using Clustal W shows that the signal sequence present at the cytoplasmic tail plays an important role in spike protein localization. A unique GYQEL motif is identified at the cytoplasmic terminal of OC43 spike protein which helps in localization in the lysosome, and a novel KLHYT motif is identified in the cytoplasmic tail of SARS spike protein which helps in ER or ERGIC localization. This study sheds some light on the role of cytoplasmic tail of spike protein in cell-to-cell fusion, coronavirus host cell fusion and subsequent pathogenicity.</div>
</front>
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<Abstract><AbstractText>Intracellular trafficking and localization studies of spike protein from SARS and OC43 showed that SARS spike protein is localized in the ER or ERGIC compartment and OC43 spike protein is predominantly localized in the lysosome. Differential localization can be explained by signal sequence. The sequence alignment using Clustal W shows that the signal sequence present at the cytoplasmic tail plays an important role in spike protein localization. A unique GYQEL motif is identified at the cytoplasmic terminal of OC43 spike protein which helps in localization in the lysosome, and a novel KLHYT motif is identified in the cytoplasmic tail of SARS spike protein which helps in ER or ERGIC localization. This study sheds some light on the role of cytoplasmic tail of spike protein in cell-to-cell fusion, coronavirus host cell fusion and subsequent pathogenicity.</AbstractText>
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Cytoplasmic tail of coronavirus spike protein has intracellular targeting signals.

Cytoplasmic tail of coronavirus spike protein has intracellular targeting signals.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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