Complemented Palindromic Small RNAs First Discovered from SARS Coronavirus.
Identifieur interne : 000980 ( PubMed/Corpus ); précédent : 000979; suivant : 000981Complemented Palindromic Small RNAs First Discovered from SARS Coronavirus.
Auteurs : Chang Liu ; Ze Chen ; Yue Hu ; Haishuo Ji ; Deshui Yu ; Wenyuan Shen ; Siyu Li ; Jishou Ruan ; Wenjun Bu ; Shan GaoSource :
- Genes [ 2073-4425 ] ; 2018.
Abstract
In this study, we report for the first time the existence of complemented palindromic small RNAs (cpsRNAs) and propose that cpsRNAs and palindromic small RNAs (psRNAs) constitute a novel class of small RNAs. The first discovered 19-nt cpsRNA UUAACAAGCUUGUUAAAGA, named SARS-CoV-cpsR-19, was detected from a 22-bp DNA complemented palindrome TCTTTAACAAGCTTGTTAAAGA in the severe acute respiratory syndrome coronavirus (SARS-CoV) genome. The phylogenetic analysis supported that this DNA complemented palindrome originated from bat betacoronavirus. The results of RNA interference (RNAi) experiments showed that one 19-nt segment corresponding to SARS-CoV-cpsR-19 significantly induced cell apoptosis. Using this joint analysis of the molecular function and phylogeny, our results suggested that SARS-CoV-cpsR-19 could play a role in SARS-CoV infection or pathogenesis. The discovery of cpsRNAs has paved a way to find novel markers for pathogen detection and to reveal the mechanisms underlying infection or pathogenesis from a different point of view. Researchers can use cpsRNAs to study the infection or pathogenesis of pathogenic viruses when these viruses are not available. The discovery of psRNAs and cpsRNAs, as a novel class of small RNAs, also inspire researchers to investigate DNA palindromes and DNA complemented palindromes with lengths of psRNAs and cpsRNAs in viral genomes.
DOI: 10.3390/genes9090442
PubMed: 30189613
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Complemented Palindromic Small RNAs First Discovered from SARS Coronavirus.</title><author><name sortKey="Liu, Chang" sort="Liu, Chang" uniqKey="Liu C" first="Chang" last="Liu">Chang Liu</name><affiliation><nlm:affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. changliu@nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China. chenze@caas.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Hu, Yue" sort="Hu, Yue" uniqKey="Hu Y" first="Yue" last="Hu">Yue Hu</name><affiliation><nlm:affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. huyue2016@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Ji, Haishuo" sort="Ji, Haishuo" uniqKey="Ji H" first="Haishuo" last="Ji">Haishuo Ji</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. haishuo_ji@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Yu, Deshui" sort="Yu, Deshui" uniqKey="Yu D" first="Deshui" last="Yu">Deshui Yu</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. deshui_yu@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Shen, Wenyuan" sort="Shen, Wenyuan" uniqKey="Shen W" first="Wenyuan" last="Shen">Wenyuan Shen</name><affiliation><nlm:affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. shenwy@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Siyu" sort="Li, Siyu" uniqKey="Li S" first="Siyu" last="Li">Siyu Li</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. siyu_li@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Ruan, Jishou" sort="Ruan, Jishou" uniqKey="Ruan J" first="Jishou" last="Ruan">Jishou Ruan</name><affiliation><nlm:affiliation>School of Mathematical Sciences, Nankai University, Tianjin 300071, China. jsruan@nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Bu, Wenjun" sort="Bu, Wenjun" uniqKey="Bu W" first="Wenjun" last="Bu">Wenjun Bu</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. wenjunbu@nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Gao, Shan" sort="Gao, Shan" uniqKey="Gao S" first="Shan" last="Gao">Shan Gao</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. gao_shan@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30189613</idno><idno type="pmid">30189613</idno><idno type="doi">10.3390/genes9090442</idno><idno type="wicri:Area/PubMed/Corpus">000980</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000980</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Complemented Palindromic Small RNAs First Discovered from SARS Coronavirus.</title><author><name sortKey="Liu, Chang" sort="Liu, Chang" uniqKey="Liu C" first="Chang" last="Liu">Chang Liu</name><affiliation><nlm:affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. changliu@nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China. chenze@caas.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Hu, Yue" sort="Hu, Yue" uniqKey="Hu Y" first="Yue" last="Hu">Yue Hu</name><affiliation><nlm:affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. huyue2016@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Ji, Haishuo" sort="Ji, Haishuo" uniqKey="Ji H" first="Haishuo" last="Ji">Haishuo Ji</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. haishuo_ji@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Yu, Deshui" sort="Yu, Deshui" uniqKey="Yu D" first="Deshui" last="Yu">Deshui Yu</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. deshui_yu@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Shen, Wenyuan" sort="Shen, Wenyuan" uniqKey="Shen W" first="Wenyuan" last="Shen">Wenyuan Shen</name><affiliation><nlm:affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. shenwy@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Siyu" sort="Li, Siyu" uniqKey="Li S" first="Siyu" last="Li">Siyu Li</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. siyu_li@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Ruan, Jishou" sort="Ruan, Jishou" uniqKey="Ruan J" first="Jishou" last="Ruan">Jishou Ruan</name><affiliation><nlm:affiliation>School of Mathematical Sciences, Nankai University, Tianjin 300071, China. jsruan@nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Bu, Wenjun" sort="Bu, Wenjun" uniqKey="Bu W" first="Wenjun" last="Bu">Wenjun Bu</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. wenjunbu@nankai.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Gao, Shan" sort="Gao, Shan" uniqKey="Gao S" first="Shan" last="Gao">Shan Gao</name><affiliation><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. gao_shan@mail.nankai.edu.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Genes</title><idno type="ISSN">2073-4425</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, we report for the first time the existence of complemented palindromic small RNAs (cpsRNAs) and propose that cpsRNAs and palindromic small RNAs (psRNAs) constitute a novel class of small RNAs. The first discovered 19-nt cpsRNA UUAACAAGCUUGUUAAAGA, named SARS-CoV-cpsR-19, was detected from a 22-bp DNA complemented palindrome TCTTTAACAAGCTTGTTAAAGA in the severe acute respiratory syndrome coronavirus (SARS-CoV) genome. The phylogenetic analysis supported that this DNA complemented palindrome originated from bat betacoronavirus. The results of RNA interference (RNAi) experiments showed that one 19-nt segment corresponding to SARS-CoV-cpsR-19 significantly induced cell apoptosis. Using this joint analysis of the molecular function and phylogeny, our results suggested that SARS-CoV-cpsR-19 could play a role in SARS-CoV infection or pathogenesis. The discovery of cpsRNAs has paved a way to find novel markers for pathogen detection and to reveal the mechanisms underlying infection or pathogenesis from a different point of view. Researchers can use cpsRNAs to study the infection or pathogenesis of pathogenic viruses when these viruses are not available. The discovery of psRNAs and cpsRNAs, as a novel class of small RNAs, also inspire researchers to investigate DNA palindromes and DNA complemented palindromes with lengths of psRNAs and cpsRNAs in viral genomes.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30189613</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2073-4425</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>9</Issue><PubDate><Year>2018</Year><Month>Sep</Month><Day>05</Day></PubDate></JournalIssue><Title>Genes</Title><ISOAbbreviation>Genes (Basel)</ISOAbbreviation></Journal><ArticleTitle>Complemented Palindromic Small RNAs First Discovered from SARS Coronavirus.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E442</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/genes9090442</ELocationID><Abstract><AbstractText>In this study, we report for the first time the existence of complemented palindromic small RNAs (cpsRNAs) and propose that cpsRNAs and palindromic small RNAs (psRNAs) constitute a novel class of small RNAs. The first discovered 19-nt cpsRNA UUAACAAGCUUGUUAAAGA, named SARS-CoV-cpsR-19, was detected from a 22-bp DNA complemented palindrome TCTTTAACAAGCTTGTTAAAGA in the severe acute respiratory syndrome coronavirus (SARS-CoV) genome. The phylogenetic analysis supported that this DNA complemented palindrome originated from bat betacoronavirus. The results of RNA interference (RNAi) experiments showed that one 19-nt segment corresponding to SARS-CoV-cpsR-19 significantly induced cell apoptosis. Using this joint analysis of the molecular function and phylogeny, our results suggested that SARS-CoV-cpsR-19 could play a role in SARS-CoV infection or pathogenesis. The discovery of cpsRNAs has paved a way to find novel markers for pathogen detection and to reveal the mechanisms underlying infection or pathogenesis from a different point of view. Researchers can use cpsRNAs to study the infection or pathogenesis of pathogenic viruses when these viruses are not available. The discovery of psRNAs and cpsRNAs, as a novel class of small RNAs, also inspire researchers to investigate DNA palindromes and DNA complemented palindromes with lengths of psRNAs and cpsRNAs in viral genomes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Chang</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0002-5717-3370</Identifier><AffiliationInfo><Affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. changliu@nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Ze</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, China. chenze@caas.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. chenze@caas.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. huyue2016@mail.nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ji</LastName><ForeName>Haishuo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. haishuo_ji@mail.nankai.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Statistics, Nankai University, Tianjin 300071, China. haishuo_ji@mail.nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Deshui</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. deshui_yu@mail.nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Wenyuan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin 300071, China. shenwy@mail.nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Siyu</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. siyu_li@mail.nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruan</LastName><ForeName>Jishou</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>School of Mathematical Sciences, Nankai University, Tianjin 300071, China. jsruan@nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bu</LastName><ForeName>Wenjun</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. wenjunbu@nankai.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Shan</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-8919-1338</Identifier><AffiliationInfo><Affiliation>College of Life Sciences, Nankai University, Tianjin 300071, China. gao_shan@mail.nankai.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Statistics, Nankai University, Tianjin 300071, China. gao_shan@mail.nankai.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2016YFC0502304-03</GrantID><Agency>National Key Research and Development Program of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>09</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Genes (Basel)</MedlineTA><NlmUniqueID>101551097</NlmUniqueID><ISSNLinking>2073-4425</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">DNA complemented palindrome</Keyword><Keyword MajorTopicYN="N">complemented palindromic small RNA</Keyword><Keyword MajorTopicYN="N">palindromic small RNA</Keyword><Keyword MajorTopicYN="N">severe acute respiratory syndrome coronavirus</Keyword><Keyword MajorTopicYN="N">small RNA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>07</Month><Day>04</Day></PubMedPubDate><PubMedPubDate 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