Early detection of antibodies against various structural proteins of the SARS-associated coronavirus in SARS patients.
Identifieur interne : 003540 ( PubMed/Checkpoint ); précédent : 003539; suivant : 003541Early detection of antibodies against various structural proteins of the SARS-associated coronavirus in SARS patients.
Auteurs : Ho-Sheng Wu [République populaire de Chine] ; Yueh-Chun Hsieh ; Ih-Jen Su ; Ting-Hsiang Lin ; Shu-Chun Chiu ; Yu-Fen Hsu ; Jih-Hui Lin ; Mei-Ching Wang ; Jeou-Yuan Chen ; Pei-Wen Hsiao ; Geen-Dong Chang ; Andrew H-J Wang ; Hsien-Wei Ting ; Chih-Ming Chou ; Chang-Jen HuangSource :
- Journal of biomedical science [ 1021-7770 ]
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Facteurs temps, Humains, Immunoglobuline A (immunologie), Immunoglobuline A (sang), Immunoglobuline G (immunologie), Immunoglobuline G (sang), Lignée cellulaire, Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Protéines recombinantes (isolement et purification), Protéines virales structurales (génétique), Protéines virales structurales (immunologie), RT-PCR, Sensibilité et spécificité, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Technique d'immunofluorescence, Technique de Western, Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (métabolisme).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Protéines recombinantes, Protéines virales structurales, Virus du SRAS.
- immunologie : Anticorps antiviraux, Immunoglobuline A, Immunoglobuline G, Protéines recombinantes, Protéines virales structurales, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Protéines recombinantes.
- métabolisme : Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline A, Immunoglobuline G.
- Animaux, Facteurs temps, Humains, Lignée cellulaire, RT-PCR, Sensibilité et spécificité, Technique d'immunofluorescence, Technique de Western, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Blotting, Western, Cell Line, Fluorescent Antibody Technique, Humans, Immunoglobulin A (blood), Immunoglobulin A (immunology), Immunoglobulin G (blood), Immunoglobulin G (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Recombinant Proteins (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (chemistry), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (metabolism), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Time Factors, Viral Structural Proteins (genetics), Viral Structural Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin A, Immunoglobulin G.
- chemical , genetics : Recombinant Proteins, Viral Structural Proteins.
- chemical , immunology : Antibodies, Viral, Immunoglobulin A, Immunoglobulin G, Recombinant Proteins, Viral Structural Proteins.
- chemical , isolation & purification : Recombinant Proteins.
- chemistry : SARS Virus.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- metabolism : SARS Virus.
- Animals, Blotting, Western, Cell Line, Fluorescent Antibody Technique, Humans, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Time Factors.
Abstract
Severe acute respiratory syndrome (SARS), a new disease with symptoms similar to those of atypical pneumonia, raised a global alert in March 2003. Because of its relatively high transmissibility and mortality upon infection, probable SARS patients were quarantined and treated with special and intensive care. Therefore, instant and accurate laboratory confirmation of SARS-associated coronavirus (SARS-CoV) infection has become a worldwide interest. For this need, we purified recombinant proteins including the nucleocapsid (N), envelope (E), membrane (M), and truncated forms of the spike protein (S1-S7) of SARS-CoV in Escherichia coli. The six proteins N, E, M, S2, S5, and S6 were used for Western blotting (WB) to detect various immunoglobulin classes in 90 serum samples from 54 probable SARS patients. The results indicated that N was recognized in most of the sera. In some cases, S6 could be recognized as early as 2 or 3 days after illness onset, while S5 was recognized at a later stage. Furthermore, the result of recombinant-protein-based WB showed a 90% agreement with that of the whole-virus-based immunofluorescence assay. Combining WB with existing RT-PCR, the laboratory confirmation for SARS-CoV infection was greatly enhanced by 24.1%, from 48.1% (RT-PCR alone) to 72.2%. Finally, our results show that IgA antibodies against SARS-CoV can be detected within 1 week after illness onset in a few SARS patients.
DOI: 10.1007/bf02256554
PubMed: 14730215
Affiliations:
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Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines recombinantes</term><term>Protéines virales structurales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline A</term><term>Immunoglobuline G</term><term>Protéines 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Reaction</term><term>Sensitivity and Specificity</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Facteurs temps</term><term>Humains</term><term>Lignée cellulaire</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS), a new disease with symptoms similar to those of atypical pneumonia, raised a global alert in March 2003. Because of its relatively high transmissibility and mortality upon infection, probable SARS patients were quarantined and treated with special and intensive care. Therefore, instant and accurate laboratory confirmation of SARS-associated coronavirus (SARS-CoV) infection has become a worldwide interest. For this need, we purified recombinant proteins including the nucleocapsid (N), envelope (E), membrane (M), and truncated forms of the spike protein (S1-S7) of SARS-CoV in Escherichia coli. The six proteins N, E, M, S2, S5, and S6 were used for Western blotting (WB) to detect various immunoglobulin classes in 90 serum samples from 54 probable SARS patients. The results indicated that N was recognized in most of the sera. In some cases, S6 could be recognized as early as 2 or 3 days after illness onset, while S5 was recognized at a later stage. Furthermore, the result of recombinant-protein-based WB showed a 90% agreement with that of the whole-virus-based immunofluorescence assay. Combining WB with existing RT-PCR, the laboratory confirmation for SARS-CoV infection was greatly enhanced by 24.1%, from 48.1% (RT-PCR alone) to 72.2%. Finally, our results show that IgA antibodies against SARS-CoV can be detected within 1 week after illness onset in a few SARS patients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14730215</PMID><DateCompleted><Year>2004</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1021-7770</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><Issue>1</Issue><PubDate><MedlineDate>2004 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Journal of biomedical science</Title><ISOAbbreviation>J. Biomed. Sci.</ISOAbbreviation></Journal><ArticleTitle>Early detection of antibodies against various structural proteins of the SARS-associated coronavirus in SARS patients.</ArticleTitle><Pagination><MedlinePgn>117-26</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS), a new disease with symptoms similar to those of atypical pneumonia, raised a global alert in March 2003. Because of its relatively high transmissibility and mortality upon infection, probable SARS patients were quarantined and treated with special and intensive care. Therefore, instant and accurate laboratory confirmation of SARS-associated coronavirus (SARS-CoV) infection has become a worldwide interest. For this need, we purified recombinant proteins including the nucleocapsid (N), envelope (E), membrane (M), and truncated forms of the spike protein (S1-S7) of SARS-CoV in Escherichia coli. The six proteins N, E, M, S2, S5, and S6 were used for Western blotting (WB) to detect various immunoglobulin classes in 90 serum samples from 54 probable SARS patients. The results indicated that N was recognized in most of the sera. In some cases, S6 could be recognized as early as 2 or 3 days after illness onset, while S5 was recognized at a later stage. Furthermore, the result of recombinant-protein-based WB showed a 90% agreement with that of the whole-virus-based immunofluorescence assay. Combining WB with existing RT-PCR, the laboratory confirmation for SARS-CoV infection was greatly enhanced by 24.1%, from 48.1% (RT-PCR alone) to 72.2%. Finally, our results show that IgA antibodies against SARS-CoV can be detected within 1 week after illness onset in a few SARS patients.</AbstractText><CopyrightInformation>Copyright 2004 National Science Council, ROC and S. Karger AG, Basel</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Ho-Sheng</ForeName><Initials>HS</Initials><AffiliationInfo><Affiliation>Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hsieh</LastName><ForeName>Yueh-Chun</ForeName><Initials>YC</Initials></Author><Author ValidYN="Y"><LastName>Su</LastName><ForeName>Ih-Jen</ForeName><Initials>IJ</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Ting-Hsiang</ForeName><Initials>TH</Initials></Author><Author ValidYN="Y"><LastName>Chiu</LastName><ForeName>Shu-Chun</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Hsu</LastName><ForeName>Yu-Fen</ForeName><Initials>YF</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Jih-Hui</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Mei-Ching</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jeou-Yuan</ForeName><Initials>JY</Initials></Author><Author ValidYN="Y"><LastName>Hsiao</LastName><ForeName>Pei-Wen</ForeName><Initials>PW</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Geen-Dong</ForeName><Initials>GD</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Andrew H-J</ForeName><Initials>AH</Initials></Author><Author ValidYN="Y"><LastName>Ting</LastName><ForeName>Hsien-Wei</ForeName><Initials>HW</Initials></Author><Author ValidYN="Y"><LastName>Chou</LastName><ForeName>Chih-Ming</ForeName><Initials>CM</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Chang-Jen</ForeName><Initials>CJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Biomed Sci</MedlineTA><NlmUniqueID>9421567</NlmUniqueID><ISSNLinking>1021-7770</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007070">Immunoglobulin A</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015678">Viral Structural Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="Y">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007070" MajorTopicYN="N">Immunoglobulin A</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName 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UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015678" MajorTopicYN="N">Viral Structural Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2003</Year><Month>09</Month><Day>02</Day></PubMedPubDate><PubMedPubDate 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30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Cell Proteomics. 2003 May;2(5):346-56</Citation><ArticleIdList><ArticleId IdType="pubmed">12775768</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chang, Geen Dong" sort="Chang, Geen Dong" uniqKey="Chang G" first="Geen-Dong" last="Chang">Geen-Dong Chang</name><name sortKey="Chen, Jeou Yuan" sort="Chen, Jeou Yuan" uniqKey="Chen J" first="Jeou-Yuan" last="Chen">Jeou-Yuan Chen</name><name sortKey="Chiu, Shu Chun" sort="Chiu, Shu Chun" uniqKey="Chiu S" first="Shu-Chun" last="Chiu">Shu-Chun Chiu</name><name sortKey="Chou, Chih Ming" sort="Chou, Chih Ming" uniqKey="Chou C" first="Chih-Ming" last="Chou">Chih-Ming Chou</name><name sortKey="Hsiao, Pei Wen" sort="Hsiao, Pei Wen" uniqKey="Hsiao P" first="Pei-Wen" last="Hsiao">Pei-Wen Hsiao</name><name sortKey="Hsieh, Yueh Chun" sort="Hsieh, Yueh Chun" uniqKey="Hsieh Y" first="Yueh-Chun" last="Hsieh">Yueh-Chun Hsieh</name><name sortKey="Hsu, Yu Fen" sort="Hsu, Yu Fen" uniqKey="Hsu Y" first="Yu-Fen" last="Hsu">Yu-Fen Hsu</name><name sortKey="Huang, Chang Jen" sort="Huang, Chang Jen" uniqKey="Huang C" first="Chang-Jen" last="Huang">Chang-Jen Huang</name><name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name><name sortKey="Lin, Ting Hsiang" sort="Lin, Ting Hsiang" uniqKey="Lin T" first="Ting-Hsiang" last="Lin">Ting-Hsiang Lin</name><name sortKey="Su, Ih Jen" sort="Su, Ih Jen" uniqKey="Su I" first="Ih-Jen" last="Su">Ih-Jen Su</name><name sortKey="Ting, Hsien Wei" sort="Ting, Hsien Wei" uniqKey="Ting H" first="Hsien-Wei" last="Ting">Hsien-Wei Ting</name><name sortKey="Wang, Andrew H J" sort="Wang, Andrew H J" uniqKey="Wang A" first="Andrew H-J" last="Wang">Andrew H-J Wang</name><name sortKey="Wang, Mei Ching" sort="Wang, Mei Ching" uniqKey="Wang M" first="Mei-Ching" last="Wang">Mei-Ching Wang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Ho Sheng" sort="Wu, Ho Sheng" uniqKey="Wu H" first="Ho-Sheng" last="Wu">Ho-Sheng Wu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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