SrasV1, PubMed, Checkpoint, bibRecord, 003364

Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.

Identifieur interne : 003364 ( PubMed/Checkpoint ); précédent : 003363; suivant : 003365

Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.

Auteurs : Fumihiro Taguchi ; Yohko K. Shimazaki

Source :

The Journal of general virology [ 0022-1317 ] ; 2000.

RBID : pubmed:11086117

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal (immunology), Antibodies, Monoclonal (pharmacology), Antigens, Viral (chemistry), Antigens, Viral (genetics), Antigens, Viral (immunology), Antigens, Viral (metabolism), Cell Fusion, Cell Line, Epitopes (chemistry), Epitopes (genetics), Epitopes (immunology), Epitopes (metabolism), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Murine hepatitis virus (drug effects), Murine hepatitis virus (genetics), Murine hepatitis virus (immunology), Murine hepatitis virus (metabolism), Mutation (genetics), Neutralization Tests, Precipitin Tests, Receptors, Virus (antagonists & inhibitors), Receptors, Virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Viral Fusion Proteins (chemistry), Viral Fusion Proteins (genetics), Viral Fusion Proteins (immunology), Viral Fusion Proteins (metabolism).
MESH :
- chemical , antagonists & inhibitors : Receptors, Virus.
- chemical , chemistry : Antigens, Viral, Epitopes, Viral Fusion Proteins.
- chemical , genetics : Antigens, Viral, Epitopes, Membrane Glycoproteins, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , immunology : Antibodies, Monoclonal, Antigens, Viral, Epitopes, Membrane Glycoproteins, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , metabolism : Antigens, Viral, Epitopes, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , pharmacology : Antibodies, Monoclonal.
- drug effects : Murine hepatitis virus.
- genetics : Murine hepatitis virus, Mutation.
- immunology : Murine hepatitis virus.
- metabolism : Murine hepatitis virus.
- Amino Acid Sequence, Animals, Cell Fusion, Cell Line, Molecular Sequence Data, Neutralization Tests, Precipitin Tests, Spike Glycoprotein, Coronavirus.

Abstract

The monoclonal antibody (MAb) 5B19.2, which has virus-neutralizing and fusion inhibition activities, binds to an epitope (S2A) consisting of nine hydrophobic amino acids in the S2 subunit of the mouse hepatitis virus (MHV) spike (S) protein. This suggests that the S2A epitope may be involved in binding the virus to the MHV receptor and/or in virus-cell fusion. Co-immunoprecipitation analyses demonstrated that while the binding of virus to the receptor was blocked by anti-S1 MAbs, it was not blocked by the S2A antiserum, indicating that S2A was not involved in receptor-binding. The S proteins prepared in this study with mutations in the S2A epitope were either fusogenic or non-fusogenic and their fusogenicity did not correlate with the hydrophobic feature of the S2A epitope. All of these wt and mutated S proteins were similarly transported onto the cell membrane independent of their fusogenicity capability. These results suggest that S2A may mediate the fusion activity of the MHV S protein during virus entry into cells.

DOI: 10.1099/0022-1317-81-12-2867
PubMed: 11086117

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 003474
to stream PubMed, to step Curation: 003474

Links to Exploration step

pubmed:11086117

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.</title>
<author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
<affiliation><nlm:affiliation>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan1.</nlm:affiliation>
<wicri:noCountry code="subField">Japan1</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Shimazaki, Yohko K" sort="Shimazaki, Yohko K" uniqKey="Shimazaki Y" first="Yohko K" last="Shimazaki">Yohko K. Shimazaki</name>
<affiliation><nlm:affiliation>National Veterinary Assay Laboratory, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan2.</nlm:affiliation>
<wicri:noCountry code="subField">Japan2</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2000">2000</date>
<idno type="RBID">pubmed:11086117</idno>
<idno type="pmid">11086117</idno>
<idno type="doi">10.1099/0022-1317-81-12-2867</idno>
<idno type="wicri:Area/PubMed/Corpus">003474</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003474</idno>
<idno type="wicri:Area/PubMed/Curation">003474</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003474</idno>
<idno type="wicri:Area/PubMed/Checkpoint">003364</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003364</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.</title>
<author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
<affiliation><nlm:affiliation>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan1.</nlm:affiliation>
<wicri:noCountry code="subField">Japan1</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Shimazaki, Yohko K" sort="Shimazaki, Yohko K" uniqKey="Shimazaki Y" first="Yohko K" last="Shimazaki">Yohko K. Shimazaki</name>
<affiliation><nlm:affiliation>National Veterinary Assay Laboratory, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan2.</nlm:affiliation>
<wicri:noCountry code="subField">Japan2</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of general virology</title>
<idno type="ISSN">0022-1317</idno>
<imprint><date when="2000" type="published">2000</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Monoclonal (pharmacology)</term>
<term>Antigens, Viral (chemistry)</term>
<term>Antigens, Viral (genetics)</term>
<term>Antigens, Viral (immunology)</term>
<term>Antigens, Viral (metabolism)</term>
<term>Cell Fusion</term>
<term>Cell Line</term>
<term>Epitopes (chemistry)</term>
<term>Epitopes (genetics)</term>
<term>Epitopes (immunology)</term>
<term>Epitopes (metabolism)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Molecular Sequence Data</term>
<term>Murine hepatitis virus (drug effects)</term>
<term>Murine hepatitis virus (genetics)</term>
<term>Murine hepatitis virus (immunology)</term>
<term>Murine hepatitis virus (metabolism)</term>
<term>Mutation (genetics)</term>
<term>Neutralization Tests</term>
<term>Precipitin Tests</term>
<term>Receptors, Virus (antagonists & inhibitors)</term>
<term>Receptors, Virus (metabolism)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Fusion Proteins (chemistry)</term>
<term>Viral Fusion Proteins (genetics)</term>
<term>Viral Fusion Proteins (immunology)</term>
<term>Viral Fusion Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Anticorps monoclonaux (pharmacologie)</term>
<term>Antigènes viraux ()</term>
<term>Antigènes viraux (génétique)</term>
<term>Antigènes viraux (immunologie)</term>
<term>Antigènes viraux (métabolisme)</term>
<term>Données de séquences moléculaires</term>
<term>Fusion cellulaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Lignée cellulaire</term>
<term>Mutation (génétique)</term>
<term>Protéines de fusion virale ()</term>
<term>Protéines de fusion virale (génétique)</term>
<term>Protéines de fusion virale (immunologie)</term>
<term>Protéines de fusion virale (métabolisme)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Récepteurs viraux (antagonistes et inhibiteurs)</term>
<term>Récepteurs viraux (métabolisme)</term>
<term>Séquence d'acides aminés</term>
<term>Tests aux précipitines</term>
<term>Tests de neutralisation</term>
<term>Virus de l'hépatite murine ()</term>
<term>Virus de l'hépatite murine (génétique)</term>
<term>Virus de l'hépatite murine (immunologie)</term>
<term>Virus de l'hépatite murine (métabolisme)</term>
<term>Épitopes ()</term>
<term>Épitopes (génétique)</term>
<term>Épitopes (immunologie)</term>
<term>Épitopes (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Receptors, Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antigens, Viral</term>
<term>Epitopes</term>
<term>Viral Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term>
<term>Epitopes</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antigens, Viral</term>
<term>Epitopes</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Viral</term>
<term>Epitopes</term>
<term>Membrane Glycoproteins</term>
<term>Receptors, Virus</term>
<term>Viral Envelope Proteins</term>
<term>Viral Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antibodies, Monoclonal</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Récepteurs viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Murine hepatitis virus</term>
<term>Mutation</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term>
<term>Glycoprotéines membranaires</term>
<term>Mutation</term>
<term>Protéines de fusion virale</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus de l'hépatite murine</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term>
<term>Antigènes viraux</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de fusion virale</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus de l'hépatite murine</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes viraux</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de fusion virale</term>
<term>Protéines de l'enveloppe virale</term>
<term>Récepteurs viraux</term>
<term>Virus de l'hépatite murine</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Anticorps monoclonaux</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cell Fusion</term>
<term>Cell Line</term>
<term>Molecular Sequence Data</term>
<term>Neutralization Tests</term>
<term>Precipitin Tests</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Antigènes viraux</term>
<term>Données de séquences moléculaires</term>
<term>Fusion cellulaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Lignée cellulaire</term>
<term>Protéines de fusion virale</term>
<term>Séquence d'acides aminés</term>
<term>Tests aux précipitines</term>
<term>Tests de neutralisation</term>
<term>Virus de l'hépatite murine</term>
<term>Épitopes</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The monoclonal antibody (MAb) 5B19.2, which has virus-neutralizing and fusion inhibition activities, binds to an epitope (S2A) consisting of nine hydrophobic amino acids in the S2 subunit of the mouse hepatitis virus (MHV) spike (S) protein. This suggests that the S2A epitope may be involved in binding the virus to the MHV receptor and/or in virus-cell fusion. Co-immunoprecipitation analyses demonstrated that while the binding of virus to the receptor was blocked by anti-S1 MAbs, it was not blocked by the S2A antiserum, indicating that S2A was not involved in receptor-binding. The S proteins prepared in this study with mutations in the S2A epitope were either fusogenic or non-fusogenic and their fusogenicity did not correlate with the hydrophobic feature of the S2A epitope. All of these wt and mutated S proteins were similarly transported onto the cell membrane independent of their fusogenicity capability. These results suggest that S2A may mediate the fusion activity of the MHV S protein during virus entry into cells.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11086117</PMID>
<DateCompleted><Year>2001</Year>
<Month>01</Month>
<Day>11</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1317</ISSN>
<JournalIssue CitedMedium="Print"><Volume>81</Volume>
<Issue>Pt 12</Issue>
<PubDate><Year>2000</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>The Journal of general virology</Title>
<ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.</ArticleTitle>
<Pagination><MedlinePgn>2867-2871</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1099/0022-1317-81-12-2867</ELocationID>
<Abstract><AbstractText>The monoclonal antibody (MAb) 5B19.2, which has virus-neutralizing and fusion inhibition activities, binds to an epitope (S2A) consisting of nine hydrophobic amino acids in the S2 subunit of the mouse hepatitis virus (MHV) spike (S) protein. This suggests that the S2A epitope may be involved in binding the virus to the MHV receptor and/or in virus-cell fusion. Co-immunoprecipitation analyses demonstrated that while the binding of virus to the receptor was blocked by anti-S1 MAbs, it was not blocked by the S2A antiserum, indicating that S2A was not involved in receptor-binding. The S proteins prepared in this study with mutations in the S2A epitope were either fusogenic or non-fusogenic and their fusogenicity did not correlate with the hydrophobic feature of the S2A epitope. All of these wt and mutated S proteins were similarly transported onto the cell membrane independent of their fusogenicity capability. These results suggest that S2A may mediate the fusion activity of the MHV S protein during virus entry into cells.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Taguchi</LastName>
<ForeName>Fumihiro</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan1.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shimazaki</LastName>
<ForeName>Yohko K</ForeName>
<Initials>YK</Initials>
<AffiliationInfo><Affiliation>National Veterinary Assay Laboratory, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan2.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan1.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Gen Virol</MedlineTA>
<NlmUniqueID>0077340</NlmUniqueID>
<ISSNLinking>0022-1317</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000939">Epitopes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011991">Receptors, Virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014760">Viral Fusion Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002459" MajorTopicYN="Y">Cell Fusion</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006517" MajorTopicYN="N">Murine hepatitis virus</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009500" MajorTopicYN="N">Neutralization Tests</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011233" MajorTopicYN="N">Precipitin Tests</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011991" MajorTopicYN="N">Receptors, Virus</DescriptorName>
<QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014760" MajorTopicYN="N">Viral Fusion Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year>
<Month>11</Month>
<Day>22</Day>
<Hour>11</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2001</Year>
<Month>2</Month>
<Day>28</Day>
<Hour>10</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2000</Year>
<Month>11</Month>
<Day>22</Day>
<Hour>11</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">11086117</ArticleId>
<ArticleId IdType="doi">10.1099/0022-1317-81-12-2867</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list></list>
<tree><noCountry><name sortKey="Shimazaki, Yohko K" sort="Shimazaki, Yohko K" uniqKey="Shimazaki Y" first="Yohko K" last="Shimazaki">Yohko K. Shimazaki</name>
<name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003364 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 003364 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PubMed
   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:11086117
   |texte=   Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:11086117" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.

Functional analysis of an epitope in the S2 subunit of the murine coronavirus spike protein: involvement in fusion activity.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki