Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application.
Identifieur interne : 003259 ( PubMed/Checkpoint ); précédent : 003258; suivant : 003260Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application.
Auteurs : Xinwei Wu [République populaire de Chine] ; Gang Cheng ; Biao Di ; Aihua Yin ; Yunshao He ; Ming Wang ; Xinyu Zhou ; Lijuan He ; Kai Luo ; Lin DuSource :
- Chinese medical journal [ 0366-6999 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Fluorescence, Humains, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Polymerase Chain Reaction.
- virology : Severe Acute Respiratory Syndrome.
- Fluorescence, Humans.
Abstract
To establish a fluorescent polymerase chain reaction (F-PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application.
PubMed: 12890368
Affiliations:
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Links to Exploration step
pubmed:12890368Le document en format XML
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<author><name sortKey="Wu, Xinwei" sort="Wu, Xinwei" uniqKey="Wu X" first="Xinwei" last="Wu">Xinwei Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>Guangzhou Centre for Disease Control and Prevention, Guangzhou 510080, China. tom.wu@tom.com</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangzhou Centre for Disease Control and Prevention, Guangzhou 510080</wicri:regionArea>
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<author><name sortKey="Cheng, Gang" sort="Cheng, Gang" uniqKey="Cheng G" first="Gang" last="Cheng">Gang Cheng</name>
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<author><name sortKey="Di, Biao" sort="Di, Biao" uniqKey="Di B" first="Biao" last="Di">Biao Di</name>
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<author><name sortKey="Wang, Ming" sort="Wang, Ming" uniqKey="Wang M" first="Ming" last="Wang">Ming Wang</name>
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<author><name sortKey="Zhou, Xinyu" sort="Zhou, Xinyu" uniqKey="Zhou X" first="Xinyu" last="Zhou">Xinyu Zhou</name>
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<author><name sortKey="He, Lijuan" sort="He, Lijuan" uniqKey="He L" first="Lijuan" last="He">Lijuan He</name>
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<author><name sortKey="Luo, Kai" sort="Luo, Kai" uniqKey="Luo K" first="Kai" last="Luo">Kai Luo</name>
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<series><title level="j">Chinese medical journal</title>
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<term>Severe Acute Respiratory Syndrome (virology)</term>
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<front><div type="abstract" xml:lang="en">To establish a fluorescent polymerase chain reaction (F-PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application.</div>
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<DateCompleted><Year>2003</Year>
<Month>10</Month>
<Day>17</Day>
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<DateRevised><Year>2004</Year>
<Month>11</Month>
<Day>17</Day>
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<Title>Chinese medical journal</Title>
<ISOAbbreviation>Chin. Med. J.</ISOAbbreviation>
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<ArticleTitle>Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application.</ArticleTitle>
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<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To establish a fluorescent polymerase chain reaction (F-PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">The primers and the fluorescence-labeled probe were designed and synthesized according to the published sequence of the SARS-associated coronavirus genes. A F-PCR diagnosis kit for detecting the coronavirus was developed, and 115 clinical nasopharyngeal gargling liquid samples were tested.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The sequence of PCR amplified products completely matched the related sequence of the SARS-associated coronavirus genome. Forty-nine out of 67 samples from identified SARS patients and 8 of 18 samples from persons having close contact with SARS patients showed positive results. All 30 samples from healthy controls were negative.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The F-PCR method established may be a rapid, accurate and efficient way for screening and for the early diagnosis of SARS patients.</AbstractText>
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