Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application.
Identifieur interne : 003259 ( PubMed/Checkpoint ); précédent : 003258; suivant : 003260Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application.
Auteurs : Xinwei Wu [République populaire de Chine] ; Gang Cheng ; Biao Di ; Aihua Yin ; Yunshao He ; Ming Wang ; Xinyu Zhou ; Lijuan He ; Kai Luo ; Lin DuSource :
- Chinese medical journal [ 0366-6999 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Fluorescence, Humains, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- MESH :
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Polymerase Chain Reaction.
- virology : Severe Acute Respiratory Syndrome.
- Fluorescence, Humans.
Abstract
To establish a fluorescent polymerase chain reaction (F-PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application.
PubMed: 12890368
Affiliations:
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last="Di">Biao Di</name></author><author><name sortKey="Yin, Aihua" sort="Yin, Aihua" uniqKey="Yin A" first="Aihua" last="Yin">Aihua Yin</name></author><author><name sortKey="He, Yunshao" sort="He, Yunshao" uniqKey="He Y" first="Yunshao" last="He">Yunshao He</name></author><author><name sortKey="Wang, Ming" sort="Wang, Ming" uniqKey="Wang M" first="Ming" last="Wang">Ming Wang</name></author><author><name sortKey="Zhou, Xinyu" sort="Zhou, Xinyu" uniqKey="Zhou X" first="Xinyu" last="Zhou">Xinyu Zhou</name></author><author><name sortKey="He, Lijuan" sort="He, Lijuan" uniqKey="He L" first="Lijuan" last="He">Lijuan He</name></author><author><name sortKey="Luo, Kai" sort="Luo, Kai" uniqKey="Luo K" first="Kai" last="Luo">Kai Luo</name></author><author><name sortKey="Du, Lin" sort="Du, Lin" uniqKey="Du L" first="Lin" last="Du">Lin Du</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12890368</idno><idno 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populaire de Chine</country><wicri:regionArea>Guangzhou Centre for Disease Control and Prevention, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Cheng, Gang" sort="Cheng, Gang" uniqKey="Cheng G" first="Gang" last="Cheng">Gang Cheng</name></author><author><name sortKey="Di, Biao" sort="Di, Biao" uniqKey="Di B" first="Biao" last="Di">Biao Di</name></author><author><name sortKey="Yin, Aihua" sort="Yin, Aihua" uniqKey="Yin A" first="Aihua" last="Yin">Aihua Yin</name></author><author><name sortKey="He, Yunshao" sort="He, Yunshao" uniqKey="He Y" first="Yunshao" last="He">Yunshao He</name></author><author><name sortKey="Wang, Ming" sort="Wang, Ming" uniqKey="Wang M" first="Ming" last="Wang">Ming Wang</name></author><author><name sortKey="Zhou, Xinyu" sort="Zhou, Xinyu" uniqKey="Zhou X" first="Xinyu" last="Zhou">Xinyu Zhou</name></author><author><name sortKey="He, Lijuan" sort="He, Lijuan" uniqKey="He L" first="Lijuan" last="He">Lijuan He</name></author><author><name sortKey="Luo, Kai" sort="Luo, Kai" uniqKey="Luo K" first="Kai" last="Luo">Kai Luo</name></author><author><name sortKey="Du, Lin" sort="Du, Lin" uniqKey="Du L" first="Lin" last="Du">Lin Du</name></author></analytic><series><title level="j">Chinese medical journal</title><idno type="ISSN">0366-6999</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fluorescence</term><term>Humans</term><term>Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fluorescence</term><term>Humains</term><term>Réaction de polymérisation en chaîne ()</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fluorescence</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fluorescence</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To establish a fluorescent polymerase chain reaction (F-PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12890368</PMID><DateCompleted><Year>2003</Year><Month>10</Month><Day>17</Day></DateCompleted><DateRevised><Year>2004</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0366-6999</ISSN><JournalIssue CitedMedium="Print"><Volume>116</Volume><Issue>7</Issue><PubDate><Year>2003</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Chinese medical journal</Title><ISOAbbreviation>Chin. Med. J.</ISOAbbreviation></Journal><ArticleTitle>Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application.</ArticleTitle><Pagination><MedlinePgn>988-90</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To establish a fluorescent polymerase chain reaction (F-PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The primers and the fluorescence-labeled probe were designed and synthesized according to the published sequence of the SARS-associated coronavirus genes. A F-PCR diagnosis kit for detecting the coronavirus was developed, and 115 clinical nasopharyngeal gargling liquid samples were tested.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The sequence of PCR amplified products completely matched the related sequence of the SARS-associated coronavirus genome. Forty-nine out of 67 samples from identified SARS patients and 8 of 18 samples from persons having close contact with SARS patients showed positive results. All 30 samples from healthy controls were negative.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The F-PCR method established may be a rapid, accurate and efficient way for screening and for the early diagnosis of SARS patients.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xinwei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Guangzhou Centre for Disease Control and Prevention, Guangzhou 510080, China. tom.wu@tom.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Gang</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Di</LastName><ForeName>Biao</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Aihua</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Yunshao</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Xinyu</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Lijuan</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Kai</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Lin</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Chin Med J (Engl)</MedlineTA><NlmUniqueID>7513795</NlmUniqueID><ISSNLinking>0366-6999</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005453" MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>8</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>10</Month><Day>18</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>8</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12890368</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Cheng, Gang" sort="Cheng, Gang" uniqKey="Cheng G" first="Gang" last="Cheng">Gang Cheng</name><name sortKey="Di, Biao" sort="Di, Biao" uniqKey="Di B" first="Biao" last="Di">Biao Di</name><name sortKey="Du, Lin" sort="Du, Lin" uniqKey="Du L" first="Lin" last="Du">Lin Du</name><name sortKey="He, Lijuan" sort="He, Lijuan" uniqKey="He L" first="Lijuan" last="He">Lijuan He</name><name sortKey="He, Yunshao" sort="He, Yunshao" uniqKey="He Y" first="Yunshao" last="He">Yunshao He</name><name sortKey="Luo, Kai" sort="Luo, Kai" uniqKey="Luo K" first="Kai" last="Luo">Kai Luo</name><name sortKey="Wang, Ming" sort="Wang, Ming" uniqKey="Wang M" first="Ming" last="Wang">Ming Wang</name><name sortKey="Yin, Aihua" sort="Yin, Aihua" uniqKey="Yin A" first="Aihua" last="Yin">Aihua Yin</name><name sortKey="Zhou, Xinyu" sort="Zhou, Xinyu" uniqKey="Zhou X" first="Xinyu" last="Zhou">Xinyu Zhou</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Wu, Xinwei" sort="Wu, Xinwei" uniqKey="Wu X" first="Xinwei" last="Wu">Xinwei Wu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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