[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].
Identifieur interne : 002F56 ( PubMed/Checkpoint ); précédent : 002F55; suivant : 002F57[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].
Auteurs : Xin-Yu Tan [République populaire de Chine] ; Zheng Fan ; Hua-Jin Wang ; Lei Shi ; Bin Yin ; An-Ping Ni ; Chuan Qin ; Ke Zou ; Yan Shen ; Jian-Gang Yuan ; Bo-Qin Qiang ; Xiao-Zhong PengSource :
- Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae [ 1000-503X ] ; 2003.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN viral (génétique), ARN viral (génétique), Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Escherichia coli (génétique), Génome viral, Protéines de fusion recombinantes (biosynthèse), Protéines de fusion recombinantes (génétique), Protéines nucléocapside (biosynthèse), Protéines nucléocapside (génétique), Protéines nucléocapside (isolement et purification), RT-PCR, Séquence d'acides aminés, Séquence nucléotidique, Vecteurs génétiques, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- biosynthèse : Protéines de fusion recombinantes, Protéines nucléocapside.
- génétique : ADN complémentaire, ADN viral, ARN viral, Escherichia coli, Protéines de fusion recombinantes, Protéines nucléocapside, Virus du SRAS.
- isolement et purification : Protéines nucléocapside, Virus du SRAS.
- Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Génome viral, RT-PCR, Séquence d'acides aminés, Séquence nucléotidique, Vecteurs génétiques.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary (genetics), DNA, Viral (genetics), Escherichia coli (genetics), Genetic Vectors, Genome, Viral, Molecular Sequence Data, Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (isolation & purification), RNA, Viral (genetics), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Analysis, DNA.
- MESH :
- chemical , biosynthesis : Nucleocapsid Proteins, Recombinant Fusion Proteins.
- chemical , genetics : DNA, Complementary, DNA, Viral, Nucleocapsid Proteins, RNA, Viral, Recombinant Fusion Proteins.
- genetics : Escherichia coli, SARS Virus.
- chemical , isolation & purification : Nucleocapsid Proteins, SARS Virus.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genetic Vectors, Genome, Viral, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.
PubMed: 14650145
Affiliations:
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Links to Exploration step
pubmed:14650145Le document en format XML
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Acta Academiae Medicinae Sinicae</title><idno type="ISSN">1000-503X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>DNA, Complementary (genetics)</term><term>DNA, Viral (genetics)</term><term>Escherichia coli (genetics)</term><term>Genetic Vectors</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (biosynthesis)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>RNA, Viral (genetics)</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN viral (génétique)</term><term>ARN viral (génétique)</term><term>Analyse de séquence d'ADN</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Génome viral</term><term>Protéines de fusion recombinantes (biosynthèse)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines nucléocapside (biosynthèse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (isolement et purification)</term><term>RT-PCR</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Vecteurs génétiques</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Viral</term><term>Nucleocapsid Proteins</term><term>RNA, Viral</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines de fusion recombinantes</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ADN viral</term><term>ARN viral</term><term>Escherichia coli</term><term>Protéines de fusion recombinantes</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Genetic Vectors</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>RT-PCR</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14650145</PMID><DateCompleted><Year>2004</Year><Month>06</Month><Day>25</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-503X</ISSN><JournalIssue CitedMedium="Print"><Volume>25</Volume><Issue>5</Issue><PubDate><Year>2003</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae</Title><ISOAbbreviation>Zhongguo Yi Xue Ke Xue Yuan Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].</ArticleTitle><Pagination><MedlinePgn>504-7</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was obtained.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Xin-yu</ForeName><Initials>XY</Initials><AffiliationInfo><Affiliation>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 10005, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Zheng</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Hua-jin</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Lei</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Bin</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Ni</LastName><ForeName>An-ping</ForeName><Initials>AP</Initials></Author><Author ValidYN="Y"><LastName>Qin</LastName><ForeName>Chuan</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Zou</LastName><ForeName>Ke</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Jian-gang</ForeName><Initials>JG</Initials></Author><Author ValidYN="Y"><LastName>Qiang</LastName><ForeName>Bo-qin</ForeName><Initials>BQ</Initials></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Xiao-zhong</ForeName><Initials>XZ</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhongguo Yi Xue Ke Xue Yuan Xue Bao</MedlineTA><NlmUniqueID>8006230</NlmUniqueID><ISSNLinking>1000-503X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="Y">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="N">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>12</Month><Day>3</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>6</Month><Day>26</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>12</Month><Day>3</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14650145</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Fan, Zheng" sort="Fan, Zheng" uniqKey="Fan Z" first="Zheng" last="Fan">Zheng Fan</name><name sortKey="Ni, An Ping" sort="Ni, An Ping" uniqKey="Ni A" first="An-Ping" last="Ni">An-Ping Ni</name><name sortKey="Peng, Xiao Zhong" sort="Peng, Xiao Zhong" uniqKey="Peng X" first="Xiao-Zhong" last="Peng">Xiao-Zhong Peng</name><name sortKey="Qiang, Bo Qin" sort="Qiang, Bo Qin" uniqKey="Qiang B" first="Bo-Qin" last="Qiang">Bo-Qin Qiang</name><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name><name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name><name sortKey="Shi, Lei" sort="Shi, Lei" uniqKey="Shi L" first="Lei" last="Shi">Lei Shi</name><name sortKey="Wang, Hua Jin" sort="Wang, Hua Jin" uniqKey="Wang H" first="Hua-Jin" last="Wang">Hua-Jin Wang</name><name sortKey="Yin, Bin" sort="Yin, Bin" uniqKey="Yin B" first="Bin" last="Yin">Bin Yin</name><name sortKey="Yuan, Jian Gang" sort="Yuan, Jian Gang" uniqKey="Yuan J" first="Jian-Gang" last="Yuan">Jian-Gang Yuan</name><name sortKey="Zou, Ke" sort="Zou, Ke" uniqKey="Zou K" first="Ke" last="Zou">Ke Zou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Tan, Xin Yu" sort="Tan, Xin Yu" uniqKey="Tan X" first="Xin-Yu" 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