SrasV1, PubMed, Checkpoint, bibRecord, 002F42

[Expression, purification and identification of recombinant SARS coronavirus membrane protein].

Identifieur interne : 002F42 ( PubMed/Checkpoint ); précédent : 002F41; suivant : 002F43

[Expression, purification and identification of recombinant SARS coronavirus membrane protein].

Auteurs : Xiao-Li Zhang [République populaire de Chine] ; Jing-Ru Wang ; Yan Zhang ; Mao-Lin Chen ; Wei Zhang ; Sheng Yang ; Wei-Hong Jiang

Source :

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica [ 0582-9879 ] ; 2003.

RBID : pubmed:14673508

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Blotting, Western, Carrier Proteins (genetics), Carrier Proteins (isolation & purification), Carrier Proteins (metabolism), Gene Expression Regulation, Viral, Maltose-Binding Proteins, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (isolation & purification), Recombinant Fusion Proteins (metabolism), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Sequence Analysis, Protein, Viral Matrix Proteins (chemistry), Viral Matrix Proteins (genetics), Viral Matrix Proteins (metabolism).
MESH :
- chemical , chemistry : Viral Matrix Proteins.
- chemical , genetics : Carrier Proteins, Recombinant Fusion Proteins, Viral Matrix Proteins.
- chemical , isolation & purification : Carrier Proteins, Recombinant Fusion Proteins, Recombinant Proteins.
- chemical , metabolism : Carrier Proteins, Recombinant Fusion Proteins, Recombinant Proteins, Viral Matrix Proteins.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- Amino Acid Sequence, Blotting, Western, Gene Expression Regulation, Viral, Maltose-Binding Proteins, Sequence Analysis, Protein.

Abstract

A novel coronavirus (SARS-coronavirus, SARS-CoV) was discovered as the pathogen of the severe acute respiratory syndrome (SARS). According to studies with other coronaviruses, the membrane protein (M protein) is the main structural protein and the recombinant M protein may be useful as an antigen for detecting antibodies against coronavirus and for preparing vaccine. In this work, the M protein of SARS-CoV was expressed in E. coli as fusion protein with maltose binding protein at N-terminus and MxeGyrA intein CBD at C-terminus. The recombinant protein was identified by Western blot and mass spectrometry. The soluble parts of the cell crude extract were then partially purified by MBP affinity chromatography. The purified protein will be used for the studies on M protein's structure and the development of diagnostic method of SARS.

PubMed: 14673508

Affiliations:

République populaire de Chine

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pubmed:14673508

Le document en format XML

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<author><name sortKey="Zhang, Xiao Li" sort="Zhang, Xiao Li" uniqKey="Zhang X" first="Xiao-Li" last="Zhang">Xiao-Li Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032</wicri:regionArea>
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<author><name sortKey="Wang, Jing Ru" sort="Wang, Jing Ru" uniqKey="Wang J" first="Jing-Ru" last="Wang">Jing-Ru Wang</name>
</author>
<author><name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name>
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<author><name sortKey="Chen, Mao Lin" sort="Chen, Mao Lin" uniqKey="Chen M" first="Mao-Lin" last="Chen">Mao-Lin Chen</name>
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<author><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name>
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<author><name sortKey="Yang, Sheng" sort="Yang, Sheng" uniqKey="Yang S" first="Sheng" last="Yang">Sheng Yang</name>
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<author><name sortKey="Chen, Mao Lin" sort="Chen, Mao Lin" uniqKey="Chen M" first="Mao-Lin" last="Chen">Mao-Lin Chen</name>
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<term>Gene Expression Regulation, Viral</term>
<term>Maltose-Binding Proteins</term>
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<term>Protéines de fusion recombinantes (métabolisme)</term>
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<term>Protéines de liaison au maltose</term>
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<term>Virus du SRAS (métabolisme)</term>
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<term>Protéines recombinantes</term>
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<term>Gene Expression Regulation, Viral</term>
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<term>Sequence Analysis, Protein</term>
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<term>Protéines de la matrice virale</term>
<term>Protéines de liaison au maltose</term>
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<front><div type="abstract" xml:lang="en">A novel coronavirus (SARS-coronavirus, SARS-CoV) was discovered as the pathogen of the severe acute respiratory syndrome (SARS). According to studies with other coronaviruses, the membrane protein (M protein) is the main structural protein and the recombinant M protein may be useful as an antigen for detecting antibodies against coronavirus and for preparing vaccine. In this work, the M protein of SARS-CoV was expressed in E. coli as fusion protein with maltose binding protein at N-terminus and MxeGyrA intein CBD at C-terminus. The recombinant protein was identified by Western blot and mass spectrometry. The soluble parts of the cell crude extract were then partially purified by MBP affinity chromatography. The purified protein will be used for the studies on M protein's structure and the development of diagnostic method of SARS.</div>
</front>
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<Abstract><AbstractText>A novel coronavirus (SARS-coronavirus, SARS-CoV) was discovered as the pathogen of the severe acute respiratory syndrome (SARS). According to studies with other coronaviruses, the membrane protein (M protein) is the main structural protein and the recombinant M protein may be useful as an antigen for detecting antibodies against coronavirus and for preparing vaccine. In this work, the M protein of SARS-CoV was expressed in E. coli as fusion protein with maltose binding protein at N-terminus and MxeGyrA intein CBD at C-terminus. The recombinant protein was identified by Western blot and mass spectrometry. The soluble parts of the cell crude extract were then partially purified by MBP affinity chromatography. The purified protein will be used for the studies on M protein's structure and the development of diagnostic method of SARS.</AbstractText>
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[Expression, purification and identification of recombinant SARS coronavirus membrane protein].

[Expression, purification and identification of recombinant SARS coronavirus membrane protein].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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