Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase.
Identifieur interne : 002E12 ( PubMed/Checkpoint ); précédent : 002E11; suivant : 002E13Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase.
Auteurs : Aili Lu [République populaire de Chine] ; Huanqing Zhang ; Xiaoyan Zhang ; Hongxia Wang ; Qikuan Hu ; Li Shen ; Brian S. Schaffhausen ; Weimin Hou ; Linsong LiSource :
- Virology [ 0042-6822 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : RNA replicase.
- enzymologie : Virus du SRAS.
- génétique : RNA replicase.
- Animaux, Cellules HeLa, Cellules Vero, Données de séquences moléculaires, Humains, Interférence par ARN, Plasmides, Syndrome respiratoire aigu sévère, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : RNA Replicase.
- chemical , genetics : RNA Replicase.
- enzymology : SARS Virus.
- therapy : Severe Acute Respiratory Syndrome.
- Animals, Base Sequence, Chlorocebus aethiops, HeLa Cells, Humans, Molecular Sequence Data, Plasmids, RNA Interference, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is a highly contagious and sometimes a lethal disease, which spread over five continents in 2002-2003. Laboratory analysis showed that the etiologic agent for SARS is a new type of coronavirus. Currently, there is no specific treatment for this disease. RNA interference (RNAi) is a recently discovered antiviral mechanism in plant and animal cells that induces a specific degradation of double-stranded RNA. Here, we provide evidences that RNAi targeting at coronavirus RNA-dependent RNA polymerase (RDRP) using short hairpin RNA (shRNA) expression plasmids can specifically inhibit expression of extraneous coronavirus RDRP in 293 and HeLa cells. Moreover, this construct significantly reduced the plaque formation of SARS coronaviruses in Vero-E6 cells. The data may suggest a new approach for treatment of SARS patients.
DOI: 10.1016/j.virol.2004.03.031
PubMed: 15183056
Affiliations:
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Schaffhausen</name></author><author><name sortKey="Hou, Weimin" sort="Hou, Weimin" uniqKey="Hou W" first="Weimin" last="Hou">Weimin Hou</name></author><author><name sortKey="Li, Linsong" sort="Li, Linsong" uniqKey="Li L" first="Linsong" last="Li">Linsong Li</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Chlorocebus aethiops</term><term>HeLa Cells</term><term>Humans</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>RNA Interference</term><term>RNA Replicase (antagonists & inhibitors)</term><term>RNA Replicase (genetics)</term><term>SARS Virus (enzymology)</term><term>Severe Acute Respiratory Syndrome (therapy)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Interférence par ARN</term><term>Plasmides</term><term>RNA replicase (antagonistes et inhibiteurs)</term><term>RNA replicase (génétique)</term><term>Syndrome respiratoire aigu sévère ()</term><term>Séquence nucléotidique</term><term>Virus du SRAS (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>RNA Replicase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA Replicase</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>RNA replicase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>RNA replicase</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Chlorocebus aethiops</term><term>HeLa Cells</term><term>Humans</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>RNA Interference</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Interférence par ARN</term><term>Plasmides</term><term>Syndrome respiratoire aigu sévère</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a highly contagious and sometimes a lethal disease, which spread over five continents in 2002-2003. Laboratory analysis showed that the etiologic agent for SARS is a new type of coronavirus. Currently, there is no specific treatment for this disease. RNA interference (RNAi) is a recently discovered antiviral mechanism in plant and animal cells that induces a specific degradation of double-stranded RNA. Here, we provide evidences that RNAi targeting at coronavirus RNA-dependent RNA polymerase (RDRP) using short hairpin RNA (shRNA) expression plasmids can specifically inhibit expression of extraneous coronavirus RDRP in 293 and HeLa cells. Moreover, this construct significantly reduced the plaque formation of SARS coronaviruses in Vero-E6 cells. The data may suggest a new approach for treatment of SARS patients.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15183056</PMID><DateCompleted><Year>2004</Year><Month>07</Month><Day>29</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0042-6822</ISSN><JournalIssue CitedMedium="Print"><Volume>324</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Jun</Month><Day>20</Day></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase.</ArticleTitle><Pagination><MedlinePgn>84-9</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is a highly contagious and sometimes a lethal disease, which spread over five continents in 2002-2003. Laboratory analysis showed that the etiologic agent for SARS is a new type of coronavirus. Currently, there is no specific treatment for this disease. RNA interference (RNAi) is a recently discovered antiviral mechanism in plant and animal cells that induces a specific degradation of double-stranded RNA. Here, we provide evidences that RNAi targeting at coronavirus RNA-dependent RNA polymerase (RDRP) using short hairpin RNA (shRNA) expression plasmids can specifically inhibit expression of extraneous coronavirus RDRP in 293 and HeLa cells. Moreover, this construct significantly reduced the plaque formation of SARS coronaviruses in Vero-E6 cells. 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ValidYN="Y"><LastName>Schaffhausen</LastName><ForeName>Brian S</ForeName><Initials>BS</Initials></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>Weimin</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Linsong</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virology</MedlineTA><NlmUniqueID>0110674</NlmUniqueID><ISSNLinking>0042-6822</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 2.7.7.48</RegistryNumber><NameOfSubstance UI="D012324">RNA Replicase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" 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Schaffhausen</name><name sortKey="Shen, Li" sort="Shen, Li" uniqKey="Shen L" first="Li" last="Shen">Li Shen</name><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name><name sortKey="Zhang, Huanqing" sort="Zhang, Huanqing" uniqKey="Zhang H" first="Huanqing" last="Zhang">Huanqing Zhang</name><name sortKey="Zhang, Xiaoyan" sort="Zhang, Xiaoyan" uniqKey="Zhang X" first="Xiaoyan" last="Zhang">Xiaoyan Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lu, Aili" sort="Lu, Aili" uniqKey="Lu A" first="Aili" last="Lu">Aili Lu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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