Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus.
Identifieur interne : 002C65 ( PubMed/Checkpoint ); précédent : 002C64; suivant : 002C66Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus.
Auteurs : Chia-Jung Yu [Taïwan] ; Yee-Chun Chen ; Cheng-Hsiang Hsiao ; Tse-Chun Kuo ; Shin C. Chang ; Chun-Yi Lu ; Wen-Chin Wei ; Chia-Huei Lee ; Li-Min Huang ; Ming-Fu Chang ; Hong-Nerng Ho ; Fang-Jen S. LeeSource :
- FEBS letters [ 0014-5793 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN complémentaire (métabolisme), Alvéoles pulmonaires (virologie), Animaux, Anticorps (), Appareil de Golgi (métabolisme), Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Cytochromes de type b (), Cytoplasme (métabolisme), Escherichia coli (métabolisme), Facteurs temps, Fractions subcellulaires (métabolisme), Humains, Immunohistochimie, Lapins, Microscopie de fluorescence, Nucléocapside (métabolisme), Peptides (), Poumon (virologie), Protéines recombinantes (), Protéines recombinantes (métabolisme), Protéines virales (), Structure tertiaire des protéines, Virus du SRAS (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
- métabolisme : ADN complémentaire, Appareil de Golgi, Cytoplasme, Escherichia coli, Fractions subcellulaires, Nucléocapside, Protéines recombinantes, Virus du SRAS.
- virologie : Alvéoles pulmonaires, Poumon.
- Animaux, Anticorps, Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Cytochromes de type b, Facteurs temps, Humains, Immunohistochimie, Lapins, Microscopie de fluorescence, Peptides, Protéines recombinantes, Protéines virales, Structure tertiaire des protéines, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals, Antibodies (chemistry), Chlorocebus aethiops, Cloning, Molecular, Cytochrome b Group (chemistry), Cytoplasm (metabolism), DNA, Complementary (metabolism), Electrophoresis, Polyacrylamide Gel, Escherichia coli (metabolism), Golgi Apparatus (metabolism), Humans, Immunohistochemistry, Lung (virology), Microscopy, Fluorescence, Nucleocapsid (metabolism), Open Reading Frames, Peptides (chemistry), Protein Structure, Tertiary, Pulmonary Alveoli (virology), Rabbits, Recombinant Proteins (chemistry), Recombinant Proteins (metabolism), SARS Virus (metabolism), Subcellular Fractions (metabolism), Time Factors, Vero Cells, Viral Proteins (chemistry).
- MESH :
- chemical , chemistry : Antibodies, Cytochrome b Group, Peptides, Recombinant Proteins, Viral Proteins.
- metabolism : Cytoplasm, DNA, Complementary, Escherichia coli, Golgi Apparatus, Nucleocapsid, Recombinant Proteins, SARS Virus, Subcellular Fractions.
- virology : Lung, Pulmonary Alveoli.
- Animals, Chlorocebus aethiops, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Humans, Immunohistochemistry, Microscopy, Fluorescence, Open Reading Frames, Protein Structure, Tertiary, Rabbits, Time Factors, Vero Cells.
Abstract
The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261-274 of the putative protein. Anti-P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in Escherichia coli and in Vero E6 cells. Expression of SARS protein 3a was detected at 8-12 h after infection and reached a higher level after approximately 24 h in SARS-CoV-infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra-alveolar cells of a SARS-CoV-infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS-CoV-infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS-CoV indeed expresses a novel protein 3a, which is present only in SARS-CoV and not in other known CoVs.
DOI: 10.1016/j.febslet.2004.03.086
PubMed: 15135062
Affiliations:
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Lee</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies (chemistry)</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Cytochrome b Group (chemistry)</term><term>Cytoplasm (metabolism)</term><term>DNA, Complementary (metabolism)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Escherichia coli (metabolism)</term><term>Golgi Apparatus (metabolism)</term><term>Humans</term><term>Immunohistochemistry</term><term>Lung (virology)</term><term>Microscopy, Fluorescence</term><term>Nucleocapsid (metabolism)</term><term>Open Reading Frames</term><term>Peptides (chemistry)</term><term>Protein Structure, Tertiary</term><term>Pulmonary Alveoli (virology)</term><term>Rabbits</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (metabolism)</term><term>Subcellular Fractions (metabolism)</term><term>Time Factors</term><term>Vero Cells</term><term>Viral Proteins (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (métabolisme)</term><term>Alvéoles pulmonaires (virologie)</term><term>Animaux</term><term>Anticorps ()</term><term>Appareil de Golgi (métabolisme)</term><term>Cadres ouverts de lecture</term><term>Cellules Vero</term><term>Clonage moléculaire</term><term>Cytochromes de type b ()</term><term>Cytoplasme (métabolisme)</term><term>Escherichia coli (métabolisme)</term><term>Facteurs temps</term><term>Fractions subcellulaires (métabolisme)</term><term>Humains</term><term>Immunohistochimie</term><term>Lapins</term><term>Microscopie de fluorescence</term><term>Nucléocapside (métabolisme)</term><term>Peptides ()</term><term>Poumon (virologie)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines virales ()</term><term>Structure tertiaire des protéines</term><term>Virus du SRAS (métabolisme)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies</term><term>Cytochrome b Group</term><term>Peptides</term><term>Recombinant Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cytoplasm</term><term>DNA, Complementary</term><term>Escherichia coli</term><term>Golgi Apparatus</term><term>Nucleocapsid</term><term>Recombinant Proteins</term><term>SARS Virus</term><term>Subcellular Fractions</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN complémentaire</term><term>Appareil de Golgi</term><term>Cytoplasme</term><term>Escherichia coli</term><term>Fractions subcellulaires</term><term>Nucléocapside</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Alvéoles pulmonaires</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term><term>Pulmonary Alveoli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Humans</term><term>Immunohistochemistry</term><term>Microscopy, Fluorescence</term><term>Open Reading Frames</term><term>Protein Structure, Tertiary</term><term>Rabbits</term><term>Time Factors</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps</term><term>Cadres ouverts de lecture</term><term>Cellules Vero</term><term>Clonage moléculaire</term><term>Cytochromes de type b</term><term>Facteurs temps</term><term>Humains</term><term>Immunohistochimie</term><term>Lapins</term><term>Microscopie de fluorescence</term><term>Peptides</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Structure tertiaire des protéines</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261-274 of the putative protein. Anti-P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in Escherichia coli and in Vero E6 cells. Expression of SARS protein 3a was detected at 8-12 h after infection and reached a higher level after approximately 24 h in SARS-CoV-infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra-alveolar cells of a SARS-CoV-infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS-CoV-infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS-CoV indeed expresses a novel protein 3a, which is present only in SARS-CoV and not in other known CoVs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15135062</PMID><DateCompleted><Year>2004</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0014-5793</ISSN><JournalIssue CitedMedium="Print"><Volume>565</Volume><Issue>1-3</Issue><PubDate><Year>2004</Year><Month>May</Month><Day>07</Day></PubDate></JournalIssue><Title>FEBS letters</Title><ISOAbbreviation>FEBS Lett.</ISOAbbreviation></Journal><ArticleTitle>Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus.</ArticleTitle><Pagination><MedlinePgn>111-6</MedlinePgn></Pagination><Abstract><AbstractText>The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261-274 of the putative protein. Anti-P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in Escherichia coli and in Vero E6 cells. Expression of SARS protein 3a was detected at 8-12 h after infection and reached a higher level after approximately 24 h in SARS-CoV-infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra-alveolar cells of a SARS-CoV-infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS-CoV-infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS-CoV indeed expresses a novel protein 3a, which is present only in SARS-CoV and not in other known CoVs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Chia-Jung</ForeName><Initials>CJ</Initials><AffiliationInfo><Affiliation>Department of Medical Research, National Taiwan University Hospital, 7 Chung Shan South Road, Taipei 100, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yee-Chun</ForeName><Initials>YC</Initials></Author><Author ValidYN="Y"><LastName>Hsiao</LastName><ForeName>Cheng-Hsiang</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>Kuo</LastName><ForeName>Tse-Chun</ForeName><Initials>TC</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Shin C</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Chun-Yi</ForeName><Initials>CY</Initials></Author><Author ValidYN="Y"><LastName>Wei</LastName><ForeName>Wen-Chin</ForeName><Initials>WC</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Chia-Huei</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Li-Min</ForeName><Initials>LM</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Ming-Fu</ForeName><Initials>MF</Initials></Author><Author ValidYN="Y"><LastName>Ho</LastName><ForeName>Hong-Nerng</ForeName><Initials>HN</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Fang-Jen S</ForeName><Initials>FJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEBS Lett</MedlineTA><NlmUniqueID>0155157</NlmUniqueID><ISSNLinking>0014-5793</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C487105">3a protein, severe acute respiratory syndrome coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000906">Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003573">Cytochrome b Group</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>11130-51-1</RegistryNumber><NameOfSubstance UI="C023646">cytochrome b561</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003573" MajorTopicYN="N">Cytochrome b Group</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006056" MajorTopicYN="N">Golgi Apparatus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019251" MajorTopicYN="N">Nucleocapsid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011650" MajorTopicYN="N">Pulmonary Alveoli</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011817" MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013347" MajorTopicYN="N">Subcellular Fractions</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>01</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>03</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>03</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>5</Month><Day>12</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>6</Month><Day>24</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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Chang</name><name sortKey="Chen, Yee Chun" sort="Chen, Yee Chun" uniqKey="Chen Y" first="Yee-Chun" last="Chen">Yee-Chun Chen</name><name sortKey="Ho, Hong Nerng" sort="Ho, Hong Nerng" uniqKey="Ho H" first="Hong-Nerng" last="Ho">Hong-Nerng Ho</name><name sortKey="Hsiao, Cheng Hsiang" sort="Hsiao, Cheng Hsiang" uniqKey="Hsiao C" first="Cheng-Hsiang" last="Hsiao">Cheng-Hsiang Hsiao</name><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><name sortKey="Kuo, Tse Chun" sort="Kuo, Tse Chun" uniqKey="Kuo T" first="Tse-Chun" last="Kuo">Tse-Chun Kuo</name><name sortKey="Lee, Chia Huei" sort="Lee, Chia Huei" uniqKey="Lee C" first="Chia-Huei" last="Lee">Chia-Huei Lee</name><name sortKey="Lee, Fang Jen S" sort="Lee, Fang Jen S" uniqKey="Lee F" first="Fang-Jen S" last="Lee">Fang-Jen S. Lee</name><name sortKey="Lu, Chun Yi" sort="Lu, Chun Yi" uniqKey="Lu C" first="Chun-Yi" last="Lu">Chun-Yi Lu</name><name sortKey="Wei, Wen Chin" sort="Wei, Wen Chin" uniqKey="Wei W" first="Wen-Chin" last="Wei">Wen-Chin Wei</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Yu, Chia Jung" sort="Yu, Chia Jung" uniqKey="Yu C" first="Chia-Jung" last="Yu">Chia-Jung Yu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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