Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus.
Identifieur interne : 002C65 ( PubMed/Checkpoint ); précédent : 002C64; suivant : 002C66Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus.
Auteurs : Chia-Jung Yu [Taïwan] ; Yee-Chun Chen ; Cheng-Hsiang Hsiao ; Tse-Chun Kuo ; Shin C. Chang ; Chun-Yi Lu ; Wen-Chin Wei ; Chia-Huei Lee ; Li-Min Huang ; Ming-Fu Chang ; Hong-Nerng Ho ; Fang-Jen S. LeeSource :
- FEBS letters [ 0014-5793 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN complémentaire (métabolisme), Alvéoles pulmonaires (virologie), Animaux, Anticorps (), Appareil de Golgi (métabolisme), Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Cytochromes de type b (), Cytoplasme (métabolisme), Escherichia coli (métabolisme), Facteurs temps, Fractions subcellulaires (métabolisme), Humains, Immunohistochimie, Lapins, Microscopie de fluorescence, Nucléocapside (métabolisme), Peptides (), Poumon (virologie), Protéines recombinantes (), Protéines recombinantes (métabolisme), Protéines virales (), Structure tertiaire des protéines, Virus du SRAS (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
- métabolisme : ADN complémentaire, Appareil de Golgi, Cytoplasme, Escherichia coli, Fractions subcellulaires, Nucléocapside, Protéines recombinantes, Virus du SRAS.
- virologie : Alvéoles pulmonaires, Poumon.
- Animaux, Anticorps, Cadres ouverts de lecture, Cellules Vero, Clonage moléculaire, Cytochromes de type b, Facteurs temps, Humains, Immunohistochimie, Lapins, Microscopie de fluorescence, Peptides, Protéines recombinantes, Protéines virales, Structure tertiaire des protéines, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals, Antibodies (chemistry), Chlorocebus aethiops, Cloning, Molecular, Cytochrome b Group (chemistry), Cytoplasm (metabolism), DNA, Complementary (metabolism), Electrophoresis, Polyacrylamide Gel, Escherichia coli (metabolism), Golgi Apparatus (metabolism), Humans, Immunohistochemistry, Lung (virology), Microscopy, Fluorescence, Nucleocapsid (metabolism), Open Reading Frames, Peptides (chemistry), Protein Structure, Tertiary, Pulmonary Alveoli (virology), Rabbits, Recombinant Proteins (chemistry), Recombinant Proteins (metabolism), SARS Virus (metabolism), Subcellular Fractions (metabolism), Time Factors, Vero Cells, Viral Proteins (chemistry).
- MESH :
- chemical , chemistry : Antibodies, Cytochrome b Group, Peptides, Recombinant Proteins, Viral Proteins.
- metabolism : Cytoplasm, DNA, Complementary, Escherichia coli, Golgi Apparatus, Nucleocapsid, Recombinant Proteins, SARS Virus, Subcellular Fractions.
- virology : Lung, Pulmonary Alveoli.
- Animals, Chlorocebus aethiops, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Humans, Immunohistochemistry, Microscopy, Fluorescence, Open Reading Frames, Protein Structure, Tertiary, Rabbits, Time Factors, Vero Cells.
Abstract
The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261-274 of the putative protein. Anti-P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in Escherichia coli and in Vero E6 cells. Expression of SARS protein 3a was detected at 8-12 h after infection and reached a higher level after approximately 24 h in SARS-CoV-infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra-alveolar cells of a SARS-CoV-infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS-CoV-infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS-CoV indeed expresses a novel protein 3a, which is present only in SARS-CoV and not in other known CoVs.
DOI: 10.1016/j.febslet.2004.03.086
PubMed: 15135062
Affiliations:
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pubmed:15135062Le document en format XML
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<front><div type="abstract" xml:lang="en">The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261-274 of the putative protein. Anti-P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in Escherichia coli and in Vero E6 cells. Expression of SARS protein 3a was detected at 8-12 h after infection and reached a higher level after approximately 24 h in SARS-CoV-infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra-alveolar cells of a SARS-CoV-infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS-CoV-infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS-CoV indeed expresses a novel protein 3a, which is present only in SARS-CoV and not in other known CoVs.</div>
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<DateCompleted><Year>2004</Year>
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<Abstract><AbstractText>The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261-274 of the putative protein. Anti-P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in Escherichia coli and in Vero E6 cells. Expression of SARS protein 3a was detected at 8-12 h after infection and reached a higher level after approximately 24 h in SARS-CoV-infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra-alveolar cells of a SARS-CoV-infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS-CoV-infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS-CoV indeed expresses a novel protein 3a, which is present only in SARS-CoV and not in other known CoVs.</AbstractText>
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<Author ValidYN="Y"><LastName>Hsiao</LastName>
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<Author ValidYN="Y"><LastName>Chang</LastName>
<ForeName>Shin C</ForeName>
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