SrasV1, PubMed, Checkpoint, bibRecord, 002C28

Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.

Identifieur interne : 002C28 ( PubMed/Checkpoint ); précédent : 002C27; suivant : 002C29

Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.

Auteurs : Zhi Wang [République populaire de Chine] ; Lili Ren ; Xingang Zhao ; Tao Hung ; Anming Meng ; Jianwei Wang ; Ye-Guang Chen

Source :

Journal of virology [ 0022-538X ] ; 2004.

RBID : pubmed:15220426

Descripteurs français

KwdFr :
- ARN viral (biosynthèse), Animaux, Cellules Vero, Effet cytopathogène viral (), Humains, Interférence par ARN, Petit ARN interférent (pharmacologie), Réplication virale (), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (physiologie).
MESH :
- biosynthèse : ARN viral.
- génétique : Virus du SRAS.
- pharmacologie : Petit ARN interférent.
- physiologie : Virus du SRAS.
- Animaux, Cellules Vero, Effet cytopathogène viral, Humains, Interférence par ARN, Réplication virale, Virus du SRAS.

English descriptors

KwdEn :
- Animals, Chlorocebus aethiops, Cytopathogenic Effect, Viral (drug effects), Humans, RNA Interference, RNA, Small Interfering (pharmacology), RNA, Viral (biosynthesis), SARS Virus (drug effects), SARS Virus (genetics), SARS Virus (physiology), Vero Cells, Virus Replication (drug effects).
MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , pharmacology : RNA, Small Interfering.
- drug effects : Cytopathogenic Effect, Viral, SARS Virus, Virus Replication.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Animals, Chlorocebus aethiops, Humans, RNA Interference, Vero Cells.

Abstract

Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.

DOI: 10.1128/JVI.78.14.7523-7527.2004
PubMed: 15220426

Affiliations:

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Le document en format XML

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<term>Humans</term>
<term>RNA Interference</term>
<term>RNA, Small Interfering (pharmacology)</term>
<term>RNA, Viral (biosynthesis)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
<term>Vero Cells</term>
<term>Virus Replication (drug effects)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (biosynthèse)</term>
<term>Animaux</term>
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<term>Effet cytopathogène viral ()</term>
<term>Humains</term>
<term>Interférence par ARN</term>
<term>Petit ARN interférent (pharmacologie)</term>
<term>Réplication virale ()</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Virus Replication</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
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<term>Cellules Vero</term>
<term>Effet cytopathogène viral</term>
<term>Humains</term>
<term>Interférence par ARN</term>
<term>Réplication virale</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.</div>
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<Month>08</Month>
<Day>03</Day>
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<Month>12</Month>
<Day>10</Day>
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<Issue>14</Issue>
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<Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.</AbstractText>
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Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.

Inhibition of severe acute respiratory syndrome virus replication by small interfering RNAs in mammalian cells.

Source :

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English descriptors

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