Tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: an in-situ hybridization study of fatal cases.
Identifieur interne : 002970 ( PubMed/Checkpoint ); précédent : 002969; suivant : 002971Tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: an in-situ hybridization study of fatal cases.
Auteurs : K F To [République populaire de Chine] ; Joanna H M. Tong ; Paul K S. Chan ; Florence W L. Au ; Stephen S C. Chim ; K C Allen Chan ; Jo L K. Cheung ; Esther Y M. Liu ; Gary M K. Tse ; Anthony W I. Lo ; Y M Dennis Lo ; H K NgSource :
- The Journal of pathology [ 0022-3417 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Virus du SRAS.
- physiologie : Virus du SRAS.
- virologie : Entérocytes, Intestin grêle, Poumon, Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Humains, Hybridation in situ, Technique FISH, Tropisme.
English descriptors
- KwdEn :
- MESH :
- isolation & purification : SARS Virus.
- physiology : SARS Virus.
- virology : Enterocytes, Intestine, Small, Lung, Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Humans, In Situ Hybridization, In Situ Hybridization, Fluorescence, Tropism, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is a new human infectious disease with significant morbidity and mortality. The disease has been shown to be associated with a new coronavirus (SARS-CoV). The clinical and epidemiological aspects of SARS have been described. Moreover, the viral genome of SARS-CoV has been fully sequenced. However, much of the biological behaviour of the virus is not known and data on the tissue and cellular tropism of SARS-CoV are limited. In this study, six fatal cases of SARS were investigated for the tissue and cellular tropism of SARS-CoV using an in-situ hybridization (ISH) technique. Among all the tissues studied, positive signals were seen in pneumocytes in the lungs and surface enterocytes in the small bowel. Infected pneumocytes were further confirmed by immunofluorescence-fluorescence in-situ hybridization (FISH) analysis. These results provide important information concerning the tissue tropism of SARS-CoV, which is distinct from previously identified human coronaviruses, and suggest the possible involvement of novel receptors in this infection. Whereas the lung pathology was dominated by diffuse alveolar damage, the gut was relatively intact. These findings indicated that tissue responses to SARS-CoV infection are distinct in different organs.
DOI: 10.1002/path.1510
PubMed: 14743497
Affiliations:
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Tong</name></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></author><author><name sortKey="Au, Florence W L" sort="Au, Florence W L" uniqKey="Au F" first="Florence W L" last="Au">Florence W L. Au</name></author><author><name sortKey="Chim, Stephen S C" sort="Chim, Stephen S C" uniqKey="Chim S" first="Stephen S C" last="Chim">Stephen S C. Chim</name></author><author><name sortKey="Chan, K C Allen" sort="Chan, K C Allen" uniqKey="Chan K" first="K C Allen" last="Chan">K C Allen Chan</name></author><author><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y M" last="Liu">Esther Y M. Liu</name></author><author><name sortKey="Tse, Gary M K" sort="Tse, Gary M K" uniqKey="Tse G" first="Gary M K" last="Tse">Gary M K. 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Tong</name></author><author><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name></author><author><name sortKey="Au, Florence W L" sort="Au, Florence W L" uniqKey="Au F" first="Florence W L" last="Au">Florence W L. Au</name></author><author><name sortKey="Chim, Stephen S C" sort="Chim, Stephen S C" uniqKey="Chim S" first="Stephen S C" last="Chim">Stephen S C. Chim</name></author><author><name sortKey="Chan, K C Allen" sort="Chan, K C Allen" uniqKey="Chan K" first="K C Allen" last="Chan">K C Allen Chan</name></author><author><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y M" last="Liu">Esther Y M. Liu</name></author><author><name sortKey="Tse, Gary M K" sort="Tse, Gary M K" uniqKey="Tse G" first="Gary M K" last="Tse">Gary M K. Tse</name></author><author><name sortKey="Lo, Anthony W I" sort="Lo, Anthony W I" uniqKey="Lo A" first="Anthony W I" last="Lo">Anthony W I. Lo</name></author><author><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y M Dennis" last="Lo">Y M Dennis Lo</name></author><author><name sortKey="Ng, H K" sort="Ng, H K" uniqKey="Ng H" first="H K" last="Ng">H K Ng</name></author></analytic><series><title level="j">The Journal of pathology</title><idno type="ISSN">0022-3417</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Enterocytes (virology)</term><term>Humans</term><term>In Situ Hybridization</term><term>In Situ Hybridization, Fluorescence</term><term>Intestine, Small (virology)</term><term>Lung (virology)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Tropism</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Entérocytes (virologie)</term><term>Humains</term><term>Hybridation in situ</term><term>Intestin grêle (virologie)</term><term>Poumon (virologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique FISH</term><term>Tropisme</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Entérocytes</term><term>Intestin grêle</term><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Enterocytes</term><term>Intestine, Small</term><term>Lung</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>In Situ Hybridization</term><term>In Situ Hybridization, Fluorescence</term><term>Tropism</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Humains</term><term>Hybridation in situ</term><term>Technique FISH</term><term>Tropisme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a new human infectious disease with significant morbidity and mortality. The disease has been shown to be associated with a new coronavirus (SARS-CoV). The clinical and epidemiological aspects of SARS have been described. Moreover, the viral genome of SARS-CoV has been fully sequenced. However, much of the biological behaviour of the virus is not known and data on the tissue and cellular tropism of SARS-CoV are limited. In this study, six fatal cases of SARS were investigated for the tissue and cellular tropism of SARS-CoV using an in-situ hybridization (ISH) technique. Among all the tissues studied, positive signals were seen in pneumocytes in the lungs and surface enterocytes in the small bowel. Infected pneumocytes were further confirmed by immunofluorescence-fluorescence in-situ hybridization (FISH) analysis. These results provide important information concerning the tissue tropism of SARS-CoV, which is distinct from previously identified human coronaviruses, and suggest the possible involvement of novel receptors in this infection. Whereas the lung pathology was dominated by diffuse alveolar damage, the gut was relatively intact. These findings indicated that tissue responses to SARS-CoV infection are distinct in different organs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14743497</PMID><DateCompleted><Year>2004</Year><Month>03</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-3417</ISSN><JournalIssue CitedMedium="Print"><Volume>202</Volume><Issue>2</Issue><PubDate><Year>2004</Year><Month>Feb</Month></PubDate></JournalIssue><Title>The Journal of pathology</Title><ISOAbbreviation>J. Pathol.</ISOAbbreviation></Journal><ArticleTitle>Tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: an in-situ hybridization study of fatal cases.</ArticleTitle><Pagination><MedlinePgn>157-63</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is a new human infectious disease with significant morbidity and mortality. The disease has been shown to be associated with a new coronavirus (SARS-CoV). The clinical and epidemiological aspects of SARS have been described. Moreover, the viral genome of SARS-CoV has been fully sequenced. However, much of the biological behaviour of the virus is not known and data on the tissue and cellular tropism of SARS-CoV are limited. In this study, six fatal cases of SARS were investigated for the tissue and cellular tropism of SARS-CoV using an in-situ hybridization (ISH) technique. Among all the tissues studied, positive signals were seen in pneumocytes in the lungs and surface enterocytes in the small bowel. Infected pneumocytes were further confirmed by immunofluorescence-fluorescence in-situ hybridization (FISH) analysis. These results provide important information concerning the tissue tropism of SARS-CoV, which is distinct from previously identified human coronaviruses, and suggest the possible involvement of novel receptors in this infection. Whereas the lung pathology was dominated by diffuse alveolar damage, the gut was relatively intact. These findings indicated that tissue responses to SARS-CoV infection are distinct in different organs.</AbstractText><CopyrightInformation>Copyright 2004 John Wiley & Sons, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>To</LastName><ForeName>K F</ForeName><Initials>KF</Initials><AffiliationInfo><Affiliation>Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China. kfto@cuhk.edu.hk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>Joanna H M</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Paul K S</ForeName><Initials>PK</Initials></Author><Author ValidYN="Y"><LastName>Au</LastName><ForeName>Florence W L</ForeName><Initials>FW</Initials></Author><Author ValidYN="Y"><LastName>Chim</LastName><ForeName>Stephen S C</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>K C Allen</ForeName><Initials>KC</Initials></Author><Author ValidYN="Y"><LastName>Cheung</LastName><ForeName>Jo L K</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Esther Y M</ForeName><Initials>EY</Initials></Author><Author ValidYN="Y"><LastName>Tse</LastName><ForeName>Gary M K</ForeName><Initials>GM</Initials></Author><Author ValidYN="Y"><LastName>Lo</LastName><ForeName>Anthony W I</ForeName><Initials>AW</Initials></Author><Author ValidYN="Y"><LastName>Lo</LastName><ForeName>Y M Dennis</ForeName><Initials>YM</Initials></Author><Author ValidYN="Y"><LastName>Ng</LastName><ForeName>H K</ForeName><Initials>HK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Pathol</MedlineTA><NlmUniqueID>0204634</NlmUniqueID><ISSNLinking>0022-3417</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>J Pathol. 2004 Jun;203(2):729-30; author reply 730-1</RefSource><PMID Version="1">15141389</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020895" MajorTopicYN="N">Enterocytes</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017403" MajorTopicYN="N">In Situ Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017404" MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007421" MajorTopicYN="N">Intestine, Small</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018523" MajorTopicYN="N">Tropism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>1</Month><Day>27</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>3</Month><Day>11</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>1</Month><Day>27</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14743497</ArticleId><ArticleId IdType="doi">10.1002/path.1510</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><noCountry><name sortKey="Au, Florence W L" sort="Au, Florence W L" uniqKey="Au F" first="Florence W L" last="Au">Florence W L. Au</name><name sortKey="Chan, K C Allen" sort="Chan, K C Allen" uniqKey="Chan K" first="K C Allen" last="Chan">K C Allen Chan</name><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. Chan</name><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name><name sortKey="Chim, Stephen S C" sort="Chim, Stephen S C" uniqKey="Chim S" first="Stephen S C" last="Chim">Stephen S C. Chim</name><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y M" last="Liu">Esther Y M. Liu</name><name sortKey="Lo, Anthony W I" sort="Lo, Anthony W I" uniqKey="Lo A" first="Anthony W I" last="Lo">Anthony W I. Lo</name><name sortKey="Lo, Y M Dennis" sort="Lo, Y M Dennis" uniqKey="Lo Y" first="Y M Dennis" last="Lo">Y M Dennis Lo</name><name sortKey="Ng, H K" sort="Ng, H K" uniqKey="Ng H" first="H K" last="Ng">H K Ng</name><name sortKey="Tong, Joanna H M" sort="Tong, Joanna H M" uniqKey="Tong J" first="Joanna H M" last="Tong">Joanna H M. Tong</name><name sortKey="Tse, Gary M K" sort="Tse, Gary M K" uniqKey="Tse G" first="Gary M K" last="Tse">Gary M K. Tse</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="To, K F" sort="To, K F" uniqKey="To K" first="K F" last="To">K F To</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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