Association of RANTES with the replication of severe acute respiratory syndrome coronavirus in THP-1 cells.
Identifieur interne : 002815 ( PubMed/Checkpoint ); précédent : 002814; suivant : 002816Association of RANTES with the replication of severe acute respiratory syndrome coronavirus in THP-1 cells.
Auteurs : D. Li [Allemagne] ; N. Wu ; H. Yao ; A. Bader ; Norbert H. Brockmeyer ; P. AltmeyerSource :
- European journal of medical research [ 0949-2321 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- physiologie : Antiviraux, Chimiokine CCL5, Réplication virale, Virus du SRAS.
- virologie : Macrophages.
- Animaux, Cellules CHO, Cellules Vero, Cricetinae, Humains, Lignée cellulaire tumorale.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Antiviral Agents, Chemokine CCL5.
- physiology : SARS Virus, Virus Replication.
- virology : Macrophages.
- Animals, CHO Cells, Cell Line, Tumor, Chlorocebus aethiops, Cricetinae, Humans, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is a novel infectious disease which is characterized by an overaggressive immune response. Chemokines are important inflammatory mediators and regulate disease due to viral infection. In previous study, we found that SARS-CoV has the ability to replicate in mononuclear cells. In present work, we sought to characterize the replication of SARS-CoV at the presence of RANTES in THP-1 cells.
PubMed: 15851378
Affiliations:
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pubmed:15851378Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Department of Dermatology and Allergology, Ruhr-University Bochum, St. Josef-Hospital, Gudrunstr. 56, D-44791 Bochum, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
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<term>Chemokine CCL5 (physiology)</term>
<term>Chlorocebus aethiops</term>
<term>Cricetinae</term>
<term>Humans</term>
<term>Macrophages (virology)</term>
<term>SARS Virus (physiology)</term>
<term>Vero Cells</term>
<term>Virus Replication (physiology)</term>
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<term>Antiviraux (physiologie)</term>
<term>Cellules CHO</term>
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<term>Chimiokine CCL5 (physiologie)</term>
<term>Cricetinae</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Macrophages (virologie)</term>
<term>Réplication virale (physiologie)</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Chemokine CCL5</term>
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<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
<term>Virus Replication</term>
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<term>Humans</term>
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<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is a novel infectious disease which is characterized by an overaggressive immune response. Chemokines are important inflammatory mediators and regulate disease due to viral infection. In previous study, we found that SARS-CoV has the ability to replicate in mononuclear cells. In present work, we sought to characterize the replication of SARS-CoV at the presence of RANTES in THP-1 cells.</div>
</front>
</TEI>
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<DateCompleted><Year>2005</Year>
<Month>09</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0949-2321</ISSN>
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<Issue>3</Issue>
<PubDate><Year>2005</Year>
<Month>Mar</Month>
<Day>29</Day>
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<Title>European journal of medical research</Title>
<ISOAbbreviation>Eur. J. Med. Res.</ISOAbbreviation>
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<ArticleTitle>Association of RANTES with the replication of severe acute respiratory syndrome coronavirus in THP-1 cells.</ArticleTitle>
<Pagination><MedlinePgn>117-20</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Severe acute respiratory syndrome (SARS) is a novel infectious disease which is characterized by an overaggressive immune response. Chemokines are important inflammatory mediators and regulate disease due to viral infection. In previous study, we found that SARS-CoV has the ability to replicate in mononuclear cells. In present work, we sought to characterize the replication of SARS-CoV at the presence of RANTES in THP-1 cells.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">To determine whether RANTES play an role in the process of SARS, THP-1 cells were incubated with heat-inactivated SARS-CoV and ELISA was used to test RANTES levels in the supernatants; Then the effect of dexamethasone on the induced secretion was evaluated. Real-time PCR was used to investigate the effort of RANTES on the replication of SARS-CoV in vitro. Macrophages, induced by THP-1 cells, were used as cell model.</AbstractText>
<AbstractText Label="FINDINGS" NlmCategory="RESULTS">Inactive SARS-CoV could induce THP-1 cells secret RANTES and this increase effect could not be suppressed by DXM. RANTES itself could inhibit the replication of SARS-CoV in THP-1 cells when it was added into the culture before or at the same time with the virus; No inhibition effect was shown when RANTES were added into the culture after SARS-CoV infected the cells.</AbstractText>
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