Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum.
Identifieur interne : 002740 ( PubMed/Checkpoint ); précédent : 002739; suivant : 002741Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum.
Auteurs : S. Chen [République populaire de Chine] ; D. Lu ; M. Zhang ; J. Che ; Z. Yin ; S. Zhang ; W. Zhang ; X. Bo ; Y. Ding ; S. WangSource :
- European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [ 0934-9723 ] ; 2005.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Humains, Protéines nucléocapside (immunologie), Protéines recombinantes (analyse), Protéines recombinantes (immunologie), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (épidémiologie), Test ELISA, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- analyse : Protéines recombinantes.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Virus du SRAS.
- immunologie : Protéines nucléocapside, Protéines recombinantes, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- sang : Anticorps antiviraux.
- épidémiologie : Syndrome respiratoire aigu sévère.
- Humains, Test ELISA.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Enzyme-Linked Immunosorbent Assay, Humans, Nucleocapsid Proteins (immunology), Recombinant Proteins (analysis), Recombinant Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , analysis : Recombinant Proteins.
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Nucleocapsid Proteins, Recombinant Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- Enzyme-Linked Immunosorbent Assay, Humans.
Abstract
The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.
DOI: 10.1007/s10096-005-1378-7
PubMed: 16133409
Affiliations:
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Chen</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850</wicri:regionArea><wicri:noRegion>100850</wicri:noRegion></affiliation></author><author><name sortKey="Lu, D" sort="Lu, D" uniqKey="Lu D" first="D" last="Lu">D. Lu</name></author><author><name sortKey="Zhang, M" sort="Zhang, M" uniqKey="Zhang M" first="M" last="Zhang">M. Zhang</name></author><author><name sortKey="Che, J" sort="Che, J" uniqKey="Che J" first="J" last="Che">J. Che</name></author><author><name sortKey="Yin, Z" sort="Yin, Z" uniqKey="Yin Z" first="Z" last="Yin">Z. Yin</name></author><author><name sortKey="Zhang, S" sort="Zhang, S" uniqKey="Zhang S" first="S" last="Zhang">S. 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Wang</name></author></analytic><series><title level="j">European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology</title><idno type="ISSN">0934-9723</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Nucleocapsid Proteins (immunology)</term><term>Recombinant Proteins (analysis)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (sang)</term><term>Humains</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes (analyse)</term><term>Protéines recombinantes (immunologie)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Test ELISA</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16133409</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0934-9723</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>8</Issue><PubDate><Year>2005</Year><Month>Aug</Month></PubDate></JournalIssue><Title>European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology</Title><ISOAbbreviation>Eur. J. Clin. Microbiol. Infect. 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A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing, 100850, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Che</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Z</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Bo</LastName><ForeName>X</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>S</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Eur J Clin Microbiol Infect Dis</MedlineTA><NlmUniqueID>8804297</NlmUniqueID><ISSNLinking>0934-9723</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, 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Bo</name><name sortKey="Che, J" sort="Che, J" uniqKey="Che J" first="J" last="Che">J. Che</name><name sortKey="Ding, Y" sort="Ding, Y" uniqKey="Ding Y" first="Y" last="Ding">Y. Ding</name><name sortKey="Lu, D" sort="Lu, D" uniqKey="Lu D" first="D" last="Lu">D. Lu</name><name sortKey="Wang, S" sort="Wang, S" uniqKey="Wang S" first="S" last="Wang">S. Wang</name><name sortKey="Yin, Z" sort="Yin, Z" uniqKey="Yin Z" first="Z" last="Yin">Z. Yin</name><name sortKey="Zhang, M" sort="Zhang, M" uniqKey="Zhang M" first="M" last="Zhang">M. Zhang</name><name sortKey="Zhang, S" sort="Zhang, S" uniqKey="Zhang S" first="S" last="Zhang">S. Zhang</name><name sortKey="Zhang, W" sort="Zhang, W" uniqKey="Zhang W" first="W" last="Zhang">W. Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chen, S" sort="Chen, S" uniqKey="Chen S" first="S" last="Chen">S. Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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