Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.
Identifieur interne : 002726 ( PubMed/Checkpoint ); précédent : 002725; suivant : 002727Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.
Auteurs : Hong-Ying Mo [République populaire de Chine] ; Jun Xu ; Xiao-Lan Ren ; Guang-Qiao Zeng ; Ya-Xia Tan ; Rong-Chang Chen ; Moira Chan-Yeung ; Nan-Shan ZhongSource :
- Chinese medical journal [ 0366-6999 ] ; 2005.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Anticorps antiviraux (sang), Femelle, Humains, Immunoglobuline G (sang), Immunoglobuline M (sang), Lymphocytes T cytotoxiques (immunologie), Mâle, Sujet âgé, Syndrome respiratoire aigu sévère (immunologie), Technique d'immunofluorescence indirecte, Test ELISA, Virus du SRAS (immunologie).
- MESH :
English descriptors
- KwdEn :
- Adult, Aged, Antibodies, Viral (blood), Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G (blood), Immunoglobulin M (blood), Male, Middle Aged, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), T-Lymphocytes, Cytotoxic (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G, Immunoglobulin M.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic.
- Adult, Aged, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Male, Middle Aged.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.
PubMed: 15788124
Affiliations:
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pubmed:15788124Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.</nlm:affiliation>
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<author><name sortKey="Chen, Rong Chang" sort="Chen, Rong Chang" uniqKey="Chen R" first="Rong-Chang" last="Chen">Rong-Chang Chen</name>
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<author><name sortKey="Chan Yeung, Moira" sort="Chan Yeung, Moira" uniqKey="Chan Yeung M" first="Moira" last="Chan-Yeung">Moira Chan-Yeung</name>
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<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Female</term>
<term>Fluorescent Antibody Technique, Indirect</term>
<term>Humans</term>
<term>Immunoglobulin G (blood)</term>
<term>Immunoglobulin M (blood)</term>
<term>Male</term>
<term>Middle Aged</term>
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<term>Severe Acute Respiratory Syndrome (immunology)</term>
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<term>Anticorps antiviraux (sang)</term>
<term>Femelle</term>
<term>Humains</term>
<term>Immunoglobuline G (sang)</term>
<term>Immunoglobuline M (sang)</term>
<term>Lymphocytes T cytotoxiques (immunologie)</term>
<term>Mâle</term>
<term>Sujet âgé</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Technique d'immunofluorescence indirecte</term>
<term>Test ELISA</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Immunoglobulin G</term>
<term>Immunoglobulin M</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
<term>T-Lymphocytes, Cytotoxic</term>
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<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Immunoglobuline G</term>
<term>Immunoglobuline M</term>
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<term>Aged</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Female</term>
<term>Fluorescent Antibody Technique, Indirect</term>
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<term>Middle Aged</term>
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<term>Adulte d'âge moyen</term>
<term>Femelle</term>
<term>Humains</term>
<term>Mâle</term>
<term>Sujet âgé</term>
<term>Technique d'immunofluorescence indirecte</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.</div>
</front>
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<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15788124</PMID>
<DateCompleted><Year>2005</Year>
<Month>05</Month>
<Day>04</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0366-6999</ISSN>
<JournalIssue CitedMedium="Print"><Volume>118</Volume>
<Issue>6</Issue>
<PubDate><Year>2005</Year>
<Month>Mar</Month>
<Day>20</Day>
</PubDate>
</JournalIssue>
<Title>Chinese medical journal</Title>
<ISOAbbreviation>Chin. Med. J.</ISOAbbreviation>
</Journal>
<ArticleTitle>Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.</ArticleTitle>
<Pagination><MedlinePgn>446-50</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Two methods, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay (IFA), were used to detect specific serum IgG and IgM against SARS-CoV in 98 SARS patients and 250 controls consisting of patients with pneumonia, health-care professionals and healthy subjects. The serum antibody profiles were investigated at different times over one and a half years in 18 of the SARS patients.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">The sensitivity and specificity of ELISA for detecting IgG against SARS-CoV were 100.0% and 97.2% and for IgM 89.8% and 97.6% respectively; the figures using IFA for IgG were 100.0% and 100.0% and for IgM 81.8% and 100.0% respectively. During the first seven days of the antibodies trace test, no IgG and IgM were detected, but on day 15, IgG response increased dramatically, reaching a peak on day 60, remaining high up to day 180 and decreasing gradually until day 540. On day 15, IgM was detected, rapidly reached a peak, then declined gradually until day 180 when IgM was undetectable.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The detection of antibodies against SARS virus is helpful in the clinical diagnosis of SARS.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mo</LastName>
<ForeName>Hong-ying</ForeName>
<Initials>HY</Initials>
<AffiliationInfo><Affiliation>Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.</Affiliation>
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<Author ValidYN="Y"><LastName>Xu</LastName>
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<Author ValidYN="Y"><LastName>Ren</LastName>
<ForeName>Xiao-lan</ForeName>
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<ForeName>Moira</ForeName>
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<Author ValidYN="Y"><LastName>Zhong</LastName>
<ForeName>Nan-shan</ForeName>
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<Language>eng</Language>
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<CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Chin Med J (Engl). 2005 Mar 20;118(6):443-5</RefSource>
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<name sortKey="Ren, Xiao Lan" sort="Ren, Xiao Lan" uniqKey="Ren X" first="Xiao-Lan" last="Ren">Xiao-Lan Ren</name>
<name sortKey="Tan, Ya Xia" sort="Tan, Ya Xia" uniqKey="Tan Y" first="Ya-Xia" last="Tan">Ya-Xia Tan</name>
<name sortKey="Xu, Jun" sort="Xu, Jun" uniqKey="Xu J" first="Jun" last="Xu">Jun Xu</name>
<name sortKey="Zeng, Guang Qiao" sort="Zeng, Guang Qiao" uniqKey="Zeng G" first="Guang-Qiao" last="Zeng">Guang-Qiao Zeng</name>
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