Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.
Identifieur interne : 002726 ( PubMed/Checkpoint ); précédent : 002725; suivant : 002727Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.
Auteurs : Hong-Ying Mo [République populaire de Chine] ; Jun Xu ; Xiao-Lan Ren ; Guang-Qiao Zeng ; Ya-Xia Tan ; Rong-Chang Chen ; Moira Chan-Yeung ; Nan-Shan ZhongSource :
- Chinese medical journal [ 0366-6999 ] ; 2005.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Anticorps antiviraux (sang), Femelle, Humains, Immunoglobuline G (sang), Immunoglobuline M (sang), Lymphocytes T cytotoxiques (immunologie), Mâle, Sujet âgé, Syndrome respiratoire aigu sévère (immunologie), Technique d'immunofluorescence indirecte, Test ELISA, Virus du SRAS (immunologie).
- MESH :
English descriptors
- KwdEn :
- Adult, Aged, Antibodies, Viral (blood), Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G (blood), Immunoglobulin M (blood), Male, Middle Aged, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), T-Lymphocytes, Cytotoxic (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G, Immunoglobulin M.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic.
- Adult, Aged, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Male, Middle Aged.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.
PubMed: 15788124
Affiliations:
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last="Xu">Jun Xu</name></author><author><name sortKey="Ren, Xiao Lan" sort="Ren, Xiao Lan" uniqKey="Ren X" first="Xiao-Lan" last="Ren">Xiao-Lan Ren</name></author><author><name sortKey="Zeng, Guang Qiao" sort="Zeng, Guang Qiao" uniqKey="Zeng G" first="Guang-Qiao" last="Zeng">Guang-Qiao Zeng</name></author><author><name sortKey="Tan, Ya Xia" sort="Tan, Ya Xia" uniqKey="Tan Y" first="Ya-Xia" last="Tan">Ya-Xia Tan</name></author><author><name sortKey="Chen, Rong Chang" sort="Chen, Rong Chang" uniqKey="Chen R" first="Rong-Chang" last="Chen">Rong-Chang Chen</name></author><author><name sortKey="Chan Yeung, Moira" sort="Chan Yeung, Moira" uniqKey="Chan Yeung M" first="Moira" last="Chan-Yeung">Moira Chan-Yeung</name></author><author><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno 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Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xu, Jun" sort="Xu, Jun" uniqKey="Xu J" first="Jun" last="Xu">Jun Xu</name></author><author><name sortKey="Ren, Xiao Lan" sort="Ren, Xiao Lan" uniqKey="Ren X" first="Xiao-Lan" last="Ren">Xiao-Lan Ren</name></author><author><name sortKey="Zeng, Guang Qiao" sort="Zeng, Guang Qiao" uniqKey="Zeng G" first="Guang-Qiao" last="Zeng">Guang-Qiao Zeng</name></author><author><name sortKey="Tan, Ya Xia" sort="Tan, Ya Xia" uniqKey="Tan Y" first="Ya-Xia" last="Tan">Ya-Xia Tan</name></author><author><name sortKey="Chen, Rong Chang" sort="Chen, Rong Chang" uniqKey="Chen R" first="Rong-Chang" last="Chen">Rong-Chang Chen</name></author><author><name sortKey="Chan Yeung, Moira" sort="Chan Yeung, Moira" uniqKey="Chan Yeung M" first="Moira" last="Chan-Yeung">Moira Chan-Yeung</name></author><author><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name></author></analytic><series><title level="j">Chinese medical journal</title><idno type="ISSN">0366-6999</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Antibodies, Viral (blood)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Immunoglobulin M (blood)</term><term>Male</term><term>Middle Aged</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>T-Lymphocytes, Cytotoxic (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Anticorps antiviraux (sang)</term><term>Femelle</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Immunoglobuline M (sang)</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Mâle</term><term>Sujet âgé</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Technique d'immunofluorescence indirecte</term><term>Test ELISA</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes, Cytotoxic</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Fluorescent Antibody Technique, Indirect</term><term>Humans</term><term>Male</term><term>Middle Aged</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Sujet âgé</term><term>Technique d'immunofluorescence indirecte</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15788124</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>04</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0366-6999</ISSN><JournalIssue CitedMedium="Print"><Volume>118</Volume><Issue>6</Issue><PubDate><Year>2005</Year><Month>Mar</Month><Day>20</Day></PubDate></JournalIssue><Title>Chinese medical journal</Title><ISOAbbreviation>Chin. Med. J.</ISOAbbreviation></Journal><ArticleTitle>Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus.</ArticleTitle><Pagination><MedlinePgn>446-50</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Two methods, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay (IFA), were used to detect specific serum IgG and IgM against SARS-CoV in 98 SARS patients and 250 controls consisting of patients with pneumonia, health-care professionals and healthy subjects. The serum antibody profiles were investigated at different times over one and a half years in 18 of the SARS patients.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The sensitivity and specificity of ELISA for detecting IgG against SARS-CoV were 100.0% and 97.2% and for IgM 89.8% and 97.6% respectively; the figures using IFA for IgG were 100.0% and 100.0% and for IgM 81.8% and 100.0% respectively. During the first seven days of the antibodies trace test, no IgG and IgM were detected, but on day 15, IgG response increased dramatically, reaching a peak on day 60, remaining high up to day 180 and decreasing gradually until day 540. On day 15, IgM was detected, rapidly reached a peak, then declined gradually until day 180 when IgM was undetectable.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The detection of antibodies against SARS virus is helpful in the clinical diagnosis of SARS.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mo</LastName><ForeName>Hong-ying</ForeName><Initials>HY</Initials><AffiliationInfo><Affiliation>Guangzhou Institute of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Jun</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Xiao-lan</ForeName><Initials>XL</Initials></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Guang-qiao</ForeName><Initials>GQ</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Ya-xia</ForeName><Initials>YX</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Rong-chang</ForeName><Initials>RC</Initials></Author><Author ValidYN="Y"><LastName>Chan-Yeung</LastName><ForeName>Moira</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Nan-shan</ForeName><Initials>NS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Chin Med J (Engl)</MedlineTA><NlmUniqueID>7513795</NlmUniqueID><ISSNLinking>0366-6999</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007075">Immunoglobulin M</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Chin Med J (Engl). 2005 Mar 20;118(6):443-5</RefSource><PMID Version="1">15788123</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019084" MajorTopicYN="N">Fluorescent Antibody Technique, Indirect</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007075" MajorTopicYN="N">Immunoglobulin M</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013602" MajorTopicYN="N">T-Lymphocytes, Cytotoxic</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>3</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>5</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>3</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15788124</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Chan Yeung, Moira" sort="Chan Yeung, Moira" uniqKey="Chan Yeung M" first="Moira" last="Chan-Yeung">Moira Chan-Yeung</name><name sortKey="Chen, Rong Chang" sort="Chen, Rong Chang" uniqKey="Chen R" first="Rong-Chang" last="Chen">Rong-Chang Chen</name><name sortKey="Ren, Xiao Lan" sort="Ren, Xiao Lan" uniqKey="Ren X" first="Xiao-Lan" last="Ren">Xiao-Lan Ren</name><name sortKey="Tan, Ya Xia" sort="Tan, Ya Xia" uniqKey="Tan Y" first="Ya-Xia" last="Tan">Ya-Xia Tan</name><name sortKey="Xu, Jun" sort="Xu, Jun" uniqKey="Xu J" first="Jun" last="Xu">Jun Xu</name><name sortKey="Zeng, Guang Qiao" sort="Zeng, Guang Qiao" uniqKey="Zeng G" first="Guang-Qiao" last="Zeng">Guang-Qiao Zeng</name><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Mo, Hong Ying" sort="Mo, Hong Ying" uniqKey="Mo H" first="Hong-Ying" last="Mo">Hong-Ying Mo</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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