Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection.
Identifieur interne : 002725 ( PubMed/Checkpoint ); précédent : 002724; suivant : 002726Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection.
Auteurs : Paul K S. Chan [République populaire de Chine] ; Esther Y M. Liu ; Danny T M. Leung ; Jo L K. Cheung ; C H Ma ; Frankie C H. Tam ; Mamie Hui ; John S. Tam ; Pak Leong LimSource :
- Journal of medical virology [ 0146-6615 ] ; 2005.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Anticorps antiviraux (sang), Enfant, Enfant d'âge préscolaire, Femelle, Humains, Immunoglobuline G (sang), Mâle, Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (immunologie), Test ELISA (), Virus du SRAS (immunologie).
- MESH :
English descriptors
- KwdEn :
- Adolescent, Adult, Antibodies, Viral (blood), Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay (methods), Female, Humans, Immunoglobulin G (blood), Male, Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Nucleocapsid Proteins, Recombinant Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Enzyme-Linked Immunosorbent Assay.
- Adolescent, Adult, Child, Child, Preschool, Female, Humans, Male, Sensitivity and Specificity.
Abstract
A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein-based microtitre plate enzyme immunoassay, ELISARS is described. The results on 150 sera from SARS patients and 450 sera from non-SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large-scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti-nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.
DOI: 10.1002/jmv.20254
PubMed: 15602743
Affiliations:
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Chan</name><affiliation wicri:level="4"><nlm:affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. paulkschan@cuhk.edu.hk</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR</wicri:regionArea><orgName type="university">Université chinoise de Hong Kong</orgName><placeName><settlement type="city">Sha Tin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y M" last="Liu">Esther Y M. Liu</name></author><author><name sortKey="Leung, Danny T M" sort="Leung, Danny T M" uniqKey="Leung D" first="Danny T M" last="Leung">Danny T M. 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Tam</name></author><author><name sortKey="Lim, Pak Leong" sort="Lim, Pak Leong" uniqKey="Lim P" first="Pak Leong" last="Lim">Pak Leong Lim</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="ISSN">0146-6615</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Antibodies, Viral (blood)</term><term>Child</term><term>Child, Preschool</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Female</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Male</term><term>Nucleocapsid Proteins (immunology)</term><term>Recombinant Proteins (immunology)</term><term>SARS Virus (immunology)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Anticorps antiviraux (sang)</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Mâle</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes (immunologie)</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Test ELISA ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Male</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Sensibilité et spécificité</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. 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The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15602743</PMID><DateCompleted><Year>2005</Year><Month>01</Month><Day>31</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0146-6615</ISSN><JournalIssue CitedMedium="Print"><Volume>75</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of medical virology</Title><ISOAbbreviation>J. Med. Virol.</ISOAbbreviation></Journal><ArticleTitle>Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection.</ArticleTitle><Pagination><MedlinePgn>181-4</MedlinePgn></Pagination><Abstract><AbstractText>A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein-based microtitre plate enzyme immunoassay, ELISARS is described. The results on 150 sera from SARS patients and 450 sera from non-SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large-scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti-nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Paul K S</ForeName><Initials>PK</Initials><AffiliationInfo><Affiliation>Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. paulkschan@cuhk.edu.hk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Esther Y M</ForeName><Initials>EY</Initials></Author><Author ValidYN="Y"><LastName>Leung</LastName><ForeName>Danny T M</ForeName><Initials>DT</Initials></Author><Author ValidYN="Y"><LastName>Cheung</LastName><ForeName>Jo L K</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>C H</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>Tam</LastName><ForeName>Frankie C H</ForeName><Initials>FC</Initials></Author><Author ValidYN="Y"><LastName>Hui</LastName><ForeName>Mamie</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tam</LastName><ForeName>John S</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>Pak Leong</ForeName><Initials>PL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Med Virol</MedlineTA><NlmUniqueID>7705876</NlmUniqueID><ISSNLinking>0146-6615</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, 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PubStatus="entrez"><Year>2004</Year><Month>12</Month><Day>17</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15602743</ArticleId><ArticleId IdType="doi">10.1002/jmv.20254</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Sha Tin</li></settlement><orgName><li>Université chinoise de Hong Kong</li></orgName></list><tree><noCountry><name sortKey="Cheung, Jo L K" sort="Cheung, Jo L K" uniqKey="Cheung J" first="Jo L K" last="Cheung">Jo L K. Cheung</name><name sortKey="Hui, Mamie" sort="Hui, Mamie" uniqKey="Hui M" first="Mamie" last="Hui">Mamie Hui</name><name sortKey="Leung, Danny T M" sort="Leung, Danny T M" uniqKey="Leung D" first="Danny T M" last="Leung">Danny T M. Leung</name><name sortKey="Lim, Pak Leong" sort="Lim, Pak Leong" uniqKey="Lim P" first="Pak Leong" last="Lim">Pak Leong Lim</name><name sortKey="Liu, Esther Y M" sort="Liu, Esther Y M" uniqKey="Liu E" first="Esther Y M" last="Liu">Esther Y M. Liu</name><name sortKey="Ma, C H" sort="Ma, C H" uniqKey="Ma C" first="C H" last="Ma">C H Ma</name><name sortKey="Tam, Frankie C H" sort="Tam, Frankie C H" uniqKey="Tam F" first="Frankie C H" last="Tam">Frankie C H. Tam</name><name sortKey="Tam, John S" sort="Tam, John S" uniqKey="Tam J" first="John S" last="Tam">John S. Tam</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Chan, Paul K S" sort="Chan, Paul K S" uniqKey="Chan P" first="Paul K S" last="Chan">Paul K S. 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