Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study.
Identifieur interne : 002712 ( PubMed/Checkpoint ); précédent : 002711; suivant : 002713Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study.
Auteurs : Chia-Wei Lai [Taïwan] ; Yao-Chi Chung ; Yiu-Kay Lai ; Margaret Dah-Tsyr Chang ; Yu-Chen HuSource :
- Biotechnology letters [ 0141-5492 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Baculoviridae (génétique), Chromatographie d'affinité (), Expression des gènes, Facteurs temps, Lignée cellulaire, Microscopie confocale, Protéines de fusion recombinantes (isolement et purification), Protéines de fusion recombinantes (métabolisme), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Spodoptera, Survie cellulaire (génétique), Technique de Western, Transfection, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Baculoviridae, Protéines de l'enveloppe virale, Protéines nucléocapside, Survie cellulaire, Virus du SRAS.
- isolement et purification : Protéines de fusion recombinantes.
- métabolisme : Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Protéines nucléocapside, Virus du SRAS.
- Animaux, Chromatographie d'affinité, Expression des gènes, Facteurs temps, Lignée cellulaire, Microscopie confocale, Spodoptera, Technique de Western, Transfection.
English descriptors
- KwdEn :
- Animals, Baculoviridae (genetics), Blotting, Western, Cell Line, Cell Survival (genetics), Chromatography, Affinity (methods), Gene Expression, Microscopy, Confocal, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Recombinant Fusion Proteins (isolation & purification), Recombinant Fusion Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Spodoptera, Time Factors, Transfection, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Viral Envelope Proteins.
- genetics : Baculoviridae, Cell Survival, SARS Virus.
- chemical , isolation & purification : Recombinant Fusion Proteins.
- chemical , metabolism : Nucleocapsid Proteins, Recombinant Fusion Proteins, SARS Virus, Viral Envelope Proteins.
- methods : Chromatography, Affinity.
- Animals, Blotting, Western, Cell Line, Gene Expression, Microscopy, Confocal, Spodoptera, Time Factors, Transfection.
Abstract
Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.
DOI: 10.1007/s10529-005-7176-6
PubMed: 16091881
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:16091881Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study.</title>
<author><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, National Tsing Hua University, 300 Hsinchu, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Chemical Engineering, National Tsing Hua University, 300 Hsinchu</wicri:regionArea>
<wicri:noRegion>300 Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chung, Yao Chi" sort="Chung, Yao Chi" uniqKey="Chung Y" first="Yao-Chi" last="Chung">Yao-Chi Chung</name>
</author>
<author><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name>
</author>
<author><name sortKey="Chang, Margaret Dah Tsyr" sort="Chang, Margaret Dah Tsyr" uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name>
</author>
<author><name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:16091881</idno>
<idno type="pmid">16091881</idno>
<idno type="doi">10.1007/s10529-005-7176-6</idno>
<idno type="wicri:Area/PubMed/Corpus">002601</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002601</idno>
<idno type="wicri:Area/PubMed/Curation">002601</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002601</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002712</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002712</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study.</title>
<author><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Engineering, National Tsing Hua University, 300 Hsinchu, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Chemical Engineering, National Tsing Hua University, 300 Hsinchu</wicri:regionArea>
<wicri:noRegion>300 Hsinchu</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chung, Yao Chi" sort="Chung, Yao Chi" uniqKey="Chung Y" first="Yao-Chi" last="Chung">Yao-Chi Chung</name>
</author>
<author><name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name>
</author>
<author><name sortKey="Chang, Margaret Dah Tsyr" sort="Chang, Margaret Dah Tsyr" uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name>
</author>
<author><name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name>
</author>
</analytic>
<series><title level="j">Biotechnology letters</title>
<idno type="ISSN">0141-5492</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Baculoviridae (genetics)</term>
<term>Blotting, Western</term>
<term>Cell Line</term>
<term>Cell Survival (genetics)</term>
<term>Chromatography, Affinity (methods)</term>
<term>Gene Expression</term>
<term>Microscopy, Confocal</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Recombinant Fusion Proteins (isolation & purification)</term>
<term>Recombinant Fusion Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>Spodoptera</term>
<term>Time Factors</term>
<term>Transfection</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Baculoviridae (génétique)</term>
<term>Chromatographie d'affinité ()</term>
<term>Expression des gènes</term>
<term>Facteurs temps</term>
<term>Lignée cellulaire</term>
<term>Microscopie confocale</term>
<term>Protéines de fusion recombinantes (isolement et purification)</term>
<term>Protéines de fusion recombinantes (métabolisme)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Spodoptera</term>
<term>Survie cellulaire (génétique)</term>
<term>Technique de Western</term>
<term>Transfection</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Baculoviridae</term>
<term>Cell Survival</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Baculoviridae</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines nucléocapside</term>
<term>Survie cellulaire</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines de fusion recombinantes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Recombinant Fusion Proteins</term>
<term>SARS Virus</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Affinity</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de fusion recombinantes</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Blotting, Western</term>
<term>Cell Line</term>
<term>Gene Expression</term>
<term>Microscopy, Confocal</term>
<term>Spodoptera</term>
<term>Time Factors</term>
<term>Transfection</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Chromatographie d'affinité</term>
<term>Expression des gènes</term>
<term>Facteurs temps</term>
<term>Lignée cellulaire</term>
<term>Microscopie confocale</term>
<term>Spodoptera</term>
<term>Technique de Western</term>
<term>Transfection</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16091881</PMID>
<DateCompleted><Year>2005</Year>
<Month>10</Month>
<Day>25</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>25</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0141-5492</ISSN>
<JournalIssue CitedMedium="Print"><Volume>27</Volume>
<Issue>13</Issue>
<PubDate><Year>2005</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Biotechnology letters</Title>
<ISOAbbreviation>Biotechnol. Lett.</ISOAbbreviation>
</Journal>
<ArticleTitle>Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study.</ArticleTitle>
<Pagination><MedlinePgn>883-91</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lai</LastName>
<ForeName>Chia-Wei</ForeName>
<Initials>CW</Initials>
<AffiliationInfo><Affiliation>Department of Chemical Engineering, National Tsing Hua University, 300 Hsinchu, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chung</LastName>
<ForeName>Yao-Chi</ForeName>
<Initials>YC</Initials>
</Author>
<Author ValidYN="Y"><LastName>Lai</LastName>
<ForeName>Yiu-Kay</ForeName>
<Initials>YK</Initials>
</Author>
<Author ValidYN="Y"><LastName>Chang</LastName>
<ForeName>Margaret Dah-Tsyr</ForeName>
<Initials>MD</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hu</LastName>
<ForeName>Yu-Chen</ForeName>
<Initials>YC</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Biotechnol Lett</MedlineTA>
<NlmUniqueID>8008051</NlmUniqueID>
<ISSNLinking>0141-5492</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C501689">E protein, SARS coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016367" MajorTopicYN="N">Baculoviridae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018411" MajorTopicYN="N">Spodoptera</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year>
<Month>04</Month>
<Day>13</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2005</Year>
<Month>05</Month>
<Day>05</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>8</Month>
<Day>11</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>10</Month>
<Day>26</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>8</Month>
<Day>11</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16091881</ArticleId>
<ArticleId IdType="doi">10.1007/s10529-005-7176-6</ArticleId>
<ArticleId IdType="pmc">PMC7087856</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Chem Phys Lipids. 1995 Aug 1;77(1):65-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7586093</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochemistry. 1998 Jan 6;37(1):185-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9425038</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Cell Biol. 1993 Sep;122(6):1185-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8397214</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biotechnol Bioeng. 2001 Oct 5;75(1):104-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11536133</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem J. 2004 Oct 1;383(Pt 1):13-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15294014</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 1999 Sep;73(9):7853-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10438879</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Emerg Infect Dis. 2004 Nov;10(11):1947-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15550204</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2000 May;74(9):4319-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10756047</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Anal Biochem. 1987 Nov 1;166(2):368-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2449095</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 1996 Apr 1;218(1):52-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8615041</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Acta Biochim Biophys Sin (Shanghai). 2004 Nov;36(11):754-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15514849</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biotechnol Lett. 2003 Jun;25(12):919-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12889824</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Mol Biol. 2004 Aug 13;341(3):769-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15288785</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochemistry. 1983 Mar 29;22(7):1621-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6849872</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2003 Nov 28;311(4):870-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14623261</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Dec 3;325(1):374-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15522242</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2004 Dec 5;330(1):322-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15527857</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Taïwan</li>
</country>
</list>
<tree><noCountry><name sortKey="Chang, Margaret Dah Tsyr" sort="Chang, Margaret Dah Tsyr" uniqKey="Chang M" first="Margaret Dah-Tsyr" last="Chang">Margaret Dah-Tsyr Chang</name>
<name sortKey="Chung, Yao Chi" sort="Chung, Yao Chi" uniqKey="Chung Y" first="Yao-Chi" last="Chung">Yao-Chi Chung</name>
<name sortKey="Hu, Yu Chen" sort="Hu, Yu Chen" uniqKey="Hu Y" first="Yu-Chen" last="Hu">Yu-Chen Hu</name>
<name sortKey="Lai, Yiu Kay" sort="Lai, Yiu Kay" uniqKey="Lai Y" first="Yiu-Kay" last="Lai">Yiu-Kay Lai</name>
</noCountry>
<country name="Taïwan"><noRegion><name sortKey="Lai, Chia Wei" sort="Lai, Chia Wei" uniqKey="Lai C" first="Chia-Wei" last="Lai">Chia-Wei Lai</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002712 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002712 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= PubMed |étape= Checkpoint |type= RBID |clé= pubmed:16091881 |texte= Expression and purification of N and E proteins from severe acute respiratory syndrome (SARS)-associated coronavirus: a comparative study. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:16091881" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |