G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells.
Identifieur interne : 002701 ( PubMed/Checkpoint ); précédent : 002700; suivant : 002702G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells.
Auteurs : Xiaoling Yuan [République populaire de Chine] ; Yajun Shan ; Zhenhu Zhao ; Jiapei Chen ; Yuwen CongSource :
- Virology journal [ 1743-422X ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- pathogénicité : Virus du SRAS.
- physiologie : Protéines virales non structurales.
- Animaux, Apoptose, Cellules COS, Cellules Vero, Cytocinèse.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Viral Nonstructural Proteins.
- drug effects : Cytokinesis.
- pathogenicity : SARS Virus.
- Animals, Apoptosis, COS Cells, Chlorocebus aethiops, Vero Cells.
Abstract
Severe Acute Respiratory Syndrome coronavirus (SARS-CoV), cause of the life-threatening atypical pneumonia, infects many organs, such as lung, liver and immune organ, and induces parenchyma cells apoptosis and necrosis. The genome of SARS-CoV, not closely related to any of the previously characterized coronavirus, encodes replicase and four major structural proteins and a number of non-structural proteins. Published studies suggest that some non-structural proteins may play important roles in the replication, virulence and pathogenesis of viruses. Among the potential SARS-CoV non-structural proteins, 3b protein (ORF4) is predicted encoding 154 amino acids, lacking significant similarities to any known proteins. Till now, there is no report about the function of 3b protein. In this study, 3b gene was linked with the EGFP tag at the C- terminus. Through cell cycle analysis, it was found that over-expression of 3b-EGFP protein in Vero, 293 and COS-7 cells could induce cell cycle arrest at G0/G1 phase, and that especially in COS-7 cells, expression of 3b-EGFP was able to induce the increase of sub-G1 phase from 24 h after transfection, which was most obvious at 48 h. The apoptosis induction of 3b fusion protein in COS-7 cells was further confirmed by double cell labeling with 7-AAD and Annexin V, the function of 3b protein inducing cell G0/G1 arrest and apoptosis may provide a new insight for further study on the mechanism of SARS pathogenesis.
DOI: 10.1186/1743-422X-2-66
PubMed: 16107218
Affiliations:
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Zhao</name></author><author><name sortKey="Chen, Jiapei" sort="Chen, Jiapei" uniqKey="Chen J" first="Jiapei" last="Chen">Jiapei Chen</name></author><author><name sortKey="Cong, Yuwen" sort="Cong, Yuwen" uniqKey="Cong Y" first="Yuwen" last="Cong">Yuwen Cong</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16107218</idno><idno type="pmid">16107218</idno><idno type="doi">10.1186/1743-422X-2-66</idno><idno type="wicri:Area/PubMed/Corpus">002582</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002582</idno><idno type="wicri:Area/PubMed/Curation">002582</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002582</idno><idno type="wicri:Area/PubMed/Checkpoint">002701</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002701</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells.</title><author><name sortKey="Yuan, Xiaoling" sort="Yuan, Xiaoling" uniqKey="Yuan X" first="Xiaoling" last="Yuan">Xiaoling Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathophysiology, Beijing Institute of Radiation Medicine, Beijing 100850, China. xiaolingyuan@hotmail.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathophysiology, Beijing Institute of Radiation Medicine, Beijing 100850</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shan, Yajun" sort="Shan, Yajun" uniqKey="Shan Y" first="Yajun" last="Shan">Yajun Shan</name></author><author><name sortKey="Zhao, Zhenhu" sort="Zhao, Zhenhu" uniqKey="Zhao Z" first="Zhenhu" last="Zhao">Zhenhu Zhao</name></author><author><name sortKey="Chen, Jiapei" sort="Chen, Jiapei" uniqKey="Chen J" first="Jiapei" last="Chen">Jiapei Chen</name></author><author><name sortKey="Cong, Yuwen" sort="Cong, Yuwen" uniqKey="Cong Y" first="Yuwen" last="Cong">Yuwen Cong</name></author></analytic><series><title level="j">Virology journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Cytokinesis (drug effects)</term><term>SARS Virus (pathogenicity)</term><term>Vero Cells</term><term>Viral Nonstructural Proteins (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules COS</term><term>Cellules Vero</term><term>Cytocinèse ()</term><term>Protéines virales non structurales (physiologie)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cytokinesis</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules COS</term><term>Cellules Vero</term><term>Cytocinèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe Acute Respiratory Syndrome coronavirus (SARS-CoV), cause of the life-threatening atypical pneumonia, infects many organs, such as lung, liver and immune organ, and induces parenchyma cells apoptosis and necrosis. The genome of SARS-CoV, not closely related to any of the previously characterized coronavirus, encodes replicase and four major structural proteins and a number of non-structural proteins. Published studies suggest that some non-structural proteins may play important roles in the replication, virulence and pathogenesis of viruses. Among the potential SARS-CoV non-structural proteins, 3b protein (ORF4) is predicted encoding 154 amino acids, lacking significant similarities to any known proteins. Till now, there is no report about the function of 3b protein. In this study, 3b gene was linked with the EGFP tag at the C- terminus. Through cell cycle analysis, it was found that over-expression of 3b-EGFP protein in Vero, 293 and COS-7 cells could induce cell cycle arrest at G0/G1 phase, and that especially in COS-7 cells, expression of 3b-EGFP was able to induce the increase of sub-G1 phase from 24 h after transfection, which was most obvious at 48 h. The apoptosis induction of 3b fusion protein in COS-7 cells was further confirmed by double cell labeling with 7-AAD and Annexin V, the function of 3b protein inducing cell G0/G1 arrest and apoptosis may provide a new insight for further study on the mechanism of SARS pathogenesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16107218</PMID><DateCompleted><Year>2009</Year><Month>06</Month><Day>05</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1743-422X</ISSN><JournalIssue CitedMedium="Internet"><Volume>2</Volume><PubDate><Year>2005</Year><Month>Aug</Month><Day>17</Day></PubDate></JournalIssue><Title>Virology journal</Title><ISOAbbreviation>Virol. J.</ISOAbbreviation></Journal><ArticleTitle>G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells.</ArticleTitle><Pagination><MedlinePgn>66</MedlinePgn></Pagination><Abstract><AbstractText>Severe Acute Respiratory Syndrome coronavirus (SARS-CoV), cause of the life-threatening atypical pneumonia, infects many organs, such as lung, liver and immune organ, and induces parenchyma cells apoptosis and necrosis. The genome of SARS-CoV, not closely related to any of the previously characterized coronavirus, encodes replicase and four major structural proteins and a number of non-structural proteins. Published studies suggest that some non-structural proteins may play important roles in the replication, virulence and pathogenesis of viruses. Among the potential SARS-CoV non-structural proteins, 3b protein (ORF4) is predicted encoding 154 amino acids, lacking significant similarities to any known proteins. Till now, there is no report about the function of 3b protein. In this study, 3b gene was linked with the EGFP tag at the C- terminus. Through cell cycle analysis, it was found that over-expression of 3b-EGFP protein in Vero, 293 and COS-7 cells could induce cell cycle arrest at G0/G1 phase, and that especially in COS-7 cells, expression of 3b-EGFP was able to induce the increase of sub-G1 phase from 24 h after transfection, which was most obvious at 48 h. The apoptosis induction of 3b fusion protein in COS-7 cells was further confirmed by double cell labeling with 7-AAD and Annexin V, the function of 3b protein inducing cell G0/G1 arrest and apoptosis may provide a new insight for further study on the mechanism of SARS pathogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Xiaoling</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Pathophysiology, Beijing Institute of Radiation Medicine, Beijing 100850, China. xiaolingyuan@hotmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shan</LastName><ForeName>Yajun</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zhenhu</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jiapei</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Cong</LastName><ForeName>Yuwen</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>08</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Virol J</MedlineTA><NlmUniqueID>101231645</NlmUniqueID><ISSNLinking>1743-422X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017361">Viral Nonstructural Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C118611">glycoprotein 3b, coronavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="Y">Apoptosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019556" MajorTopicYN="N">COS Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048749" MajorTopicYN="N">Cytokinesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList><GeneralNote Owner="NLM">Original DateCompleted: 20051006</GeneralNote></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>04</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>08</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16107218</ArticleId><ArticleId IdType="pii">1743-422X-2-66</ArticleId><ArticleId IdType="doi">10.1186/1743-422X-2-66</ArticleId><ArticleId IdType="pmc">PMC1190220</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Virology. 2002 Dec 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first="Jiapei" last="Chen">Jiapei Chen</name><name sortKey="Cong, Yuwen" sort="Cong, Yuwen" uniqKey="Cong Y" first="Yuwen" last="Cong">Yuwen Cong</name><name sortKey="Shan, Yajun" sort="Shan, Yajun" uniqKey="Shan Y" first="Yajun" last="Shan">Yajun Shan</name><name sortKey="Zhao, Zhenhu" sort="Zhao, Zhenhu" uniqKey="Zhao Z" first="Zhenhu" last="Zhao">Zhenhu Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yuan, Xiaoling" sort="Yuan, Xiaoling" uniqKey="Yuan X" first="Xiaoling" last="Yuan">Xiaoling Yuan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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