Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton.
Identifieur interne : 002687 ( PubMed/Checkpoint ); précédent : 002686; suivant : 002688Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton.
Auteurs : Yu Zhong [Japon] ; Yoshiyuki Yoshinaka ; Tadahiro Takeda ; Noriko Shimizu ; Sayaka Yoshizaki ; Yoshio Inagaki ; Shinobu Matsuda ; Gisho Honda ; Nobutaka Fujii ; Naoki YamamotoSource :
- Antiviral research [ 0166-3542 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Agents antiVIH.
- métabolisme : Polygonum.
- pharmacologie : Agents antiVIH, Médicaments issus de plantes chinoises.
- Fermentation, Lignée cellulaire, Polygonum, Réplication virale, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Anti-HIV Agents.
- chemical , pharmacology : Anti-HIV Agents, Drugs, Chinese Herbal.
- chemistry : Polygonum.
- drug effects : HIV-1, Virus Replication.
- metabolism : Polygonum.
- Cell Line, Fermentation.
Abstract
A water-soluble extract of fermented Polygonum tinctorium Aiton (Polygonaceae) called Sukumo, exhibited a potent inhibitory activity against HIV type 1 in vitro. The extract potently suppressed acute HIV-1 (IIIB) infection in MT-4 cells with EC50 values of 0.5 microg/ml but exhibited low cytotoxicity to MT-4 cells even at a high concentration (CC50 > 1000 microg/ml). It also inhibited giant cell formation in co-cultures of HIV-infected cells and uninfected Molt-4 cells. Sukumo extract was found to interact with both the viral envelope glycoprotein and cellular receptors, thus blocking virus-cell binding and virus-induced syncytium formation. There was a good correlation between the extract's anti-HIV-1 activity and its inhibitory effects on HIV-1 binding. It also suppressed replication of herpes simplex virus type 1 in Vero cells with an EC 50 of 11.56 microg/ml. On the other hand, there was no appreciable activity against influenza A virus, poliovirus or SARS corona virus when tested at concentrations ranging from 3.2-400 microg/ml as shown by microscopic image analysis for cytopathic effect (CPE). Physico-chemical studies revealed that the anti-HIV activity in the extract was essentially maintained after boiling at 100 degrees C in 1N HCl or 1N NaOH, and after treatment with 100 mM NaIO4. The inhibitory activity of the extract was also not reduced after pronase digestion. The active factor in the extract is likely to be a novel compound(s) having a polyanionic substructure and a molecular weight of 10,000-50,000.
DOI: 10.1016/j.antiviral.2005.02.003
PubMed: 15911029
Affiliations:
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Aiton.</title><author><name sortKey="Zhong, Yu" sort="Zhong, Yu" uniqKey="Zhong Y" first="Yu" last="Zhong">Yu Zhong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Tokyo 113-8519, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Tokyo 113-8519</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name></author><author><name sortKey="Takeda, Tadahiro" sort="Takeda, Tadahiro" uniqKey="Takeda T" first="Tadahiro" last="Takeda">Tadahiro Takeda</name></author><author><name sortKey="Shimizu, Noriko" sort="Shimizu, Noriko" uniqKey="Shimizu N" first="Noriko" last="Shimizu">Noriko Shimizu</name></author><author><name sortKey="Yoshizaki, Sayaka" sort="Yoshizaki, Sayaka" uniqKey="Yoshizaki S" first="Sayaka" last="Yoshizaki">Sayaka Yoshizaki</name></author><author><name sortKey="Inagaki, Yoshio" sort="Inagaki, Yoshio" uniqKey="Inagaki Y" first="Yoshio" last="Inagaki">Yoshio Inagaki</name></author><author><name sortKey="Matsuda, Shinobu" sort="Matsuda, Shinobu" uniqKey="Matsuda S" first="Shinobu" last="Matsuda">Shinobu Matsuda</name></author><author><name sortKey="Honda, Gisho" sort="Honda, Gisho" uniqKey="Honda G" first="Gisho" last="Honda">Gisho Honda</name></author><author><name sortKey="Fujii, Nobutaka" sort="Fujii, Nobutaka" uniqKey="Fujii N" first="Nobutaka" last="Fujii">Nobutaka Fujii</name></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name></author></analytic><series><title level="j">Antiviral research</title><idno type="ISSN">0166-3542</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-HIV Agents (isolation & purification)</term><term>Anti-HIV Agents (pharmacology)</term><term>Cell Line</term><term>Drugs, Chinese Herbal (pharmacology)</term><term>Fermentation</term><term>HIV-1 (drug effects)</term><term>Polygonum (chemistry)</term><term>Polygonum (metabolism)</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agents antiVIH (isolement et purification)</term><term>Agents antiVIH (pharmacologie)</term><term>Fermentation</term><term>Lignée cellulaire</term><term>Médicaments issus de plantes chinoises (pharmacologie)</term><term>Polygonum ()</term><term>Polygonum (métabolisme)</term><term>Réplication virale ()</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Anti-HIV Agents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-HIV Agents</term><term>Drugs, Chinese Herbal</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Polygonum</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>HIV-1</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Agents antiVIH</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Polygonum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Polygonum</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Agents antiVIH</term><term>Médicaments issus de plantes chinoises</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Fermentation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fermentation</term><term>Lignée cellulaire</term><term>Polygonum</term><term>Réplication virale</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A water-soluble extract of fermented Polygonum tinctorium Aiton (Polygonaceae) called Sukumo, exhibited a potent inhibitory activity against HIV type 1 in vitro. The extract potently suppressed acute HIV-1 (IIIB) infection in MT-4 cells with EC50 values of 0.5 microg/ml but exhibited low cytotoxicity to MT-4 cells even at a high concentration (CC50 > 1000 microg/ml). It also inhibited giant cell formation in co-cultures of HIV-infected cells and uninfected Molt-4 cells. Sukumo extract was found to interact with both the viral envelope glycoprotein and cellular receptors, thus blocking virus-cell binding and virus-induced syncytium formation. There was a good correlation between the extract's anti-HIV-1 activity and its inhibitory effects on HIV-1 binding. It also suppressed replication of herpes simplex virus type 1 in Vero cells with an EC 50 of 11.56 microg/ml. On the other hand, there was no appreciable activity against influenza A virus, poliovirus or SARS corona virus when tested at concentrations ranging from 3.2-400 microg/ml as shown by microscopic image analysis for cytopathic effect (CPE). Physico-chemical studies revealed that the anti-HIV activity in the extract was essentially maintained after boiling at 100 degrees C in 1N HCl or 1N NaOH, and after treatment with 100 mM NaIO4. The inhibitory activity of the extract was also not reduced after pronase digestion. The active factor in the extract is likely to be a novel compound(s) having a polyanionic substructure and a molecular weight of 10,000-50,000.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15911029</PMID><DateCompleted><Year>2005</Year><Month>09</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0166-3542</ISSN><JournalIssue CitedMedium="Print"><Volume>66</Volume><Issue>2-3</Issue><PubDate><Year>2005</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Antiviral research</Title><ISOAbbreviation>Antiviral Res.</ISOAbbreviation></Journal><ArticleTitle>Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton.</ArticleTitle><Pagination><MedlinePgn>119-28</MedlinePgn></Pagination><Abstract><AbstractText>A water-soluble extract of fermented Polygonum tinctorium Aiton (Polygonaceae) called Sukumo, exhibited a potent inhibitory activity against HIV type 1 in vitro. The extract potently suppressed acute HIV-1 (IIIB) infection in MT-4 cells with EC50 values of 0.5 microg/ml but exhibited low cytotoxicity to MT-4 cells even at a high concentration (CC50 > 1000 microg/ml). It also inhibited giant cell formation in co-cultures of HIV-infected cells and uninfected Molt-4 cells. Sukumo extract was found to interact with both the viral envelope glycoprotein and cellular receptors, thus blocking virus-cell binding and virus-induced syncytium formation. There was a good correlation between the extract's anti-HIV-1 activity and its inhibitory effects on HIV-1 binding. It also suppressed replication of herpes simplex virus type 1 in Vero cells with an EC 50 of 11.56 microg/ml. On the other hand, there was no appreciable activity against influenza A virus, poliovirus or SARS corona virus when tested at concentrations ranging from 3.2-400 microg/ml as shown by microscopic image analysis for cytopathic effect (CPE). Physico-chemical studies revealed that the anti-HIV activity in the extract was essentially maintained after boiling at 100 degrees C in 1N HCl or 1N NaOH, and after treatment with 100 mM NaIO4. The inhibitory activity of the extract was also not reduced after pronase digestion. The active factor in the extract is likely to be a novel compound(s) having a polyanionic substructure and a molecular weight of 10,000-50,000.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima, Tokyo 113-8519, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoshinaka</LastName><ForeName>Yoshiyuki</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Takeda</LastName><ForeName>Tadahiro</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Shimizu</LastName><ForeName>Noriko</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Yoshizaki</LastName><ForeName>Sayaka</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Inagaki</LastName><ForeName>Yoshio</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Matsuda</LastName><ForeName>Shinobu</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Honda</LastName><ForeName>Gisho</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Fujii</LastName><ForeName>Nobutaka</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Yamamoto</LastName><ForeName>Naoki</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>03</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Antiviral Res</MedlineTA><NlmUniqueID>8109699</NlmUniqueID><ISSNLinking>0166-3542</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019380">Anti-HIV Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004365">Drugs, Chinese Herbal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019380" MajorTopicYN="N">Anti-HIV Agents</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004365" MajorTopicYN="N">Drugs, Chinese Herbal</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015497" MajorTopicYN="N">HIV-1</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028502" MajorTopicYN="N">Polygonum</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>09</Month><Day>13</Day></PubMedPubDate><PubMedPubDate 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IdType="pubmed">12820226</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2003 Apr;58(2):139-47</Citation><ArticleIdList><ArticleId IdType="pubmed">12742574</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Med Chem. 2001 Mar 1;44(5):703-14</Citation><ArticleIdList><ArticleId IdType="pubmed">11262081</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2002 Jan;53(1):19-34</Citation><ArticleIdList><ArticleId IdType="pubmed">11684313</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Gen Pharmacol. 1998 Sep;31(3):361-5</Citation><ArticleIdList><ArticleId IdType="pubmed">9703202</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 1992 May;18(1):91-103</Citation><ArticleIdList><ArticleId IdType="pubmed">1416904</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1988 Aug;85(16):6132-6</Citation><ArticleIdList><ArticleId IdType="pubmed">2457906</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Cancer Res Clin Oncol. 1987;113(5):413-6</Citation><ArticleIdList><ArticleId IdType="pubmed">2442171</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Planta Med. 1979 May;36(1):85-90</Citation><ArticleIdList><ArticleId IdType="pubmed">461559</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Anal Biochem. 1969 Jul;30(1):148-52</Citation><ArticleIdList><ArticleId IdType="pubmed">4183001</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15590-5</Citation><ArticleIdList><ArticleId IdType="pubmed">12434015</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Anticancer Res. 2001 Sep-Oct;21(5):3295-300</Citation><ArticleIdList><ArticleId IdType="pubmed">11848486</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2000 Feb;74(4):1948-60</Citation><ArticleIdList><ArticleId IdType="pubmed">10644368</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biosci Biotechnol Biochem. 2003 Jun;67(6):1199-205</Citation><ArticleIdList><ArticleId IdType="pubmed">12843643</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 1989 Jun-Jul;11(5-6):233-46</Citation><ArticleIdList><ArticleId IdType="pubmed">2478075</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gastroenterol. 2001 Jan;36(1):5-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11211212</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antimicrob Agents Chemother. 1987 Oct;31(10):1524-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2449120</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Rev Mol Cell Biol. 2000 Oct;1(1):40-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11413488</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Gen Pharmacol. 1997 Oct;29(4):497-511</Citation><ArticleIdList><ArticleId IdType="pubmed">9352294</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4185-90</Citation><ArticleIdList><ArticleId IdType="pubmed">12642669</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2000 Dec 20;278(2):412-22</Citation><ArticleIdList><ArticleId IdType="pubmed">11118364</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Fujii, Nobutaka" sort="Fujii, Nobutaka" uniqKey="Fujii N" first="Nobutaka" last="Fujii">Nobutaka Fujii</name><name sortKey="Honda, Gisho" sort="Honda, Gisho" uniqKey="Honda G" first="Gisho" last="Honda">Gisho Honda</name><name sortKey="Inagaki, Yoshio" sort="Inagaki, Yoshio" uniqKey="Inagaki Y" first="Yoshio" last="Inagaki">Yoshio Inagaki</name><name sortKey="Matsuda, Shinobu" sort="Matsuda, Shinobu" uniqKey="Matsuda S" first="Shinobu" last="Matsuda">Shinobu Matsuda</name><name sortKey="Shimizu, Noriko" sort="Shimizu, Noriko" uniqKey="Shimizu N" first="Noriko" last="Shimizu">Noriko Shimizu</name><name sortKey="Takeda, Tadahiro" sort="Takeda, Tadahiro" uniqKey="Takeda T" first="Tadahiro" last="Takeda">Tadahiro Takeda</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name><name sortKey="Yoshizaki, Sayaka" sort="Yoshizaki, Sayaka" uniqKey="Yoshizaki S" first="Sayaka" last="Yoshizaki">Sayaka Yoshizaki</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Zhong, Yu" sort="Zhong, Yu" uniqKey="Zhong Y" first="Yu" last="Zhong">Yu Zhong</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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