Inhibition of SARS-CoV replication by siRNA.
Identifieur interne : 002652 ( PubMed/Checkpoint ); précédent : 002651; suivant : 002653Inhibition of SARS-CoV replication by siRNA.
Auteurs : Chang-Jer Wu [République populaire de Chine] ; Hui-Wen Huang ; Chiu-Yi Liu ; Cheng-Fong Hong ; Yi-Lin ChanSource :
- Antiviral research [ 0166-3542 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Interférence par ARN, Petit ARN interférent (métabolisme), Petit ARN interférent (pharmacologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Régions 3' non traduites (génétique), Régions 3' non traduites (métabolisme), Réplication virale (), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Régions 3' non traduites.
- métabolisme : Glycoprotéines membranaires, Petit ARN interférent, Protéines de l'enveloppe virale, Régions 3' non traduites.
- pharmacologie : Petit ARN interférent.
- physiologie : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Glycoprotéine de spicule des coronavirus, Humains, Interférence par ARN, Réplication virale, Virus du SRAS.
English descriptors
- KwdEn :
- 3' Untranslated Regions (genetics), 3' Untranslated Regions (metabolism), Animals, Chlorocebus aethiops, Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), RNA Interference, RNA, Small Interfering (metabolism), RNA, Small Interfering (pharmacology), SARS Virus (drug effects), SARS Virus (physiology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Virus Replication (drug effects).
- MESH :
- chemical , genetics : 3' Untranslated Regions, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : 3' Untranslated Regions, Membrane Glycoproteins, RNA, Small Interfering, Viral Envelope Proteins.
- chemical , pharmacology : RNA, Small Interfering.
- drug effects : SARS Virus, Virus Replication.
- physiology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Humans, RNA Interference, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV, occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small double-stranded RNA molecules named small interfering RNA (siRNA). Recently, siRNA-induced RNA interference(RNAi) may provide a new approach to therapy for pathogenic viruses, e.g. HIV and HCV. In this study, the silencing potential of seven synthetic siRNAs against SARS-CoV leader, TRS, 3'-UTR and Spike coding sequence have been applied to explore the possibility for prevention of SARS-CoV infection. We demonstrate that siRNAs directed against Spike sequences and the 3'-UTR can inhibit the replication of SARS-CoV in Vero-E6 cells, and holds out promise for the development of an effective antiviral agent against SARS-CoV.
DOI: 10.1016/j.antiviral.2004.09.005
PubMed: 15652970
Affiliations:
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pubmed:15652970Le document en format XML
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last="Liu">Chiu-Yi Liu</name></author><author><name sortKey="Hong, Cheng Fong" sort="Hong, Cheng Fong" uniqKey="Hong C" first="Cheng-Fong" last="Hong">Cheng-Fong Hong</name></author><author><name sortKey="Chan, Yi Lin" sort="Chan, Yi Lin" uniqKey="Chan Y" first="Yi-Lin" last="Chan">Yi-Lin Chan</name></author></analytic><series><title level="j">Antiviral research</title><idno type="ISSN">0166-3542</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3' Untranslated Regions (genetics)</term><term>3' Untranslated Regions (metabolism)</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>RNA Interference</term><term>RNA, Small Interfering (metabolism)</term><term>RNA, Small Interfering (pharmacology)</term><term>SARS Virus (drug 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xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Interférence par ARN</term><term>Réplication virale</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV, occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small double-stranded RNA molecules named small interfering RNA (siRNA). Recently, siRNA-induced RNA interference(RNAi) may provide a new approach to therapy for pathogenic viruses, e.g. HIV and HCV. In this study, the silencing potential of seven synthetic siRNAs against SARS-CoV leader, TRS, 3'-UTR and Spike coding sequence have been applied to explore the possibility for prevention of SARS-CoV infection. We demonstrate that siRNAs directed against Spike sequences and the 3'-UTR can inhibit the replication of SARS-CoV in Vero-E6 cells, and holds out promise for the development of an effective antiviral agent against SARS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15652970</PMID><DateCompleted><Year>2005</Year><Month>03</Month><Day>22</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-3542</ISSN><JournalIssue CitedMedium="Print"><Volume>65</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Antiviral research</Title><ISOAbbreviation>Antiviral Res.</ISOAbbreviation></Journal><ArticleTitle>Inhibition of SARS-CoV replication by siRNA.</ArticleTitle><Pagination><MedlinePgn>45-8</MedlinePgn></Pagination><Abstract><AbstractText>Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV, occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small double-stranded RNA molecules named small interfering RNA (siRNA). Recently, siRNA-induced RNA interference(RNAi) may provide a new approach to therapy for pathogenic viruses, e.g. HIV and HCV. In this study, the silencing potential of seven synthetic siRNAs against SARS-CoV leader, TRS, 3'-UTR and Spike coding sequence have been applied to explore the possibility for prevention of SARS-CoV infection. We demonstrate that siRNAs directed against Spike sequences and the 3'-UTR can inhibit the replication of SARS-CoV in Vero-E6 cells, and holds out promise for the development of an effective antiviral agent against SARS-CoV.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Chang-Jer</ForeName><Initials>CJ</Initials><AffiliationInfo><Affiliation>Department of Food Science, National Taiwan Ocean University, Keelung, Pei Ning Road, Keelung 202, Taiwan, ROC. wuchangjer@yahoo.com.tw</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Hui-Wen</ForeName><Initials>HW</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Chiu-Yi</ForeName><Initials>CY</Initials></Author><Author ValidYN="Y"><LastName>Hong</LastName><ForeName>Cheng-Fong</ForeName><Initials>CF</Initials></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>Yi-Lin</ForeName><Initials>YL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Antiviral Res</MedlineTA><NlmUniqueID>8109699</NlmUniqueID><ISSNLinking>0166-3542</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020413">3' Untranslated Regions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064370">Spike Glycoprotein, 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uniqKey="Hong C" first="Cheng-Fong" last="Hong">Cheng-Fong Hong</name><name sortKey="Huang, Hui Wen" sort="Huang, Hui Wen" uniqKey="Huang H" first="Hui-Wen" last="Huang">Hui-Wen Huang</name><name sortKey="Liu, Chiu Yi" sort="Liu, Chiu Yi" uniqKey="Liu C" first="Chiu-Yi" last="Liu">Chiu-Yi Liu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Chang Jer" sort="Wu, Chang Jer" uniqKey="Wu C" first="Chang-Jer" last="Wu">Chang-Jer Wu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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