Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins.
Identifieur interne : 002614 ( PubMed/Checkpoint ); précédent : 002613; suivant : 002615Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins.
Auteurs : Ralph A. Tripp [États-Unis] ; Lia M. Haynes ; Deborah Moore ; Barbara Anderson ; Azaibi Tamin ; Brian H. Harcourt ; Les P. Jones ; Mamadi Yilla ; Gregory J. Babcock ; Thomas Greenough ; Donna M. Ambrosino ; Rene Alvarez ; Justin Callaway ; Sheana Cavitt ; Kurt Kamrud ; Harold Alterson ; Jonathan Smith ; Jennifer L. Harcourt ; Congrong Miao ; Raj Razdan ; James A. Comer ; Pierre E. Rollin ; Thomas G. Ksiazek ; Anthony Sanchez ; Paul A. Rota ; William J. Bellini ; Larry J. AndersonSource :
- Journal of virological methods [ 0166-0934 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (biosynthèse), Anticorps antiviraux (immunologie), Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Cartographie épitopique, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (immunologie), Protéines de la matrice virale (immunologie), Protéines nucléocapside (immunologie), Protéines virales structurales (immunologie), Spécificité des anticorps, Syndrome respiratoire aigu sévère (virologie), Tests de neutralisation, Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux, Anticorps monoclonaux.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Protéines virales structurales, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cartographie épitopique, Cellules Vero, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Spécificité des anticorps, Tests de neutralisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibodies, Viral (biosynthesis), Antibodies, Viral (immunology), Antibody Specificity, Cell Line, Chlorocebus aethiops, Epitope Mapping, Humans, Membrane Glycoproteins (immunology), Neutralization Tests, Nucleocapsid Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (immunology), Viral Matrix Proteins (immunology), Viral Structural Proteins (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Viral.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- immunology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Antibody Specificity, Cell Line, Chlorocebus aethiops, Epitope Mapping, Humans, Neutralization Tests, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.
DOI: 10.1016/j.jviromet.2005.03.021
PubMed: 15885812
Affiliations:
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pubmed:15885812Le document en format XML
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<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Viral (biosynthesis)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antibody Specificity</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Epitope Mapping</term>
<term>Humans</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Neutralization Tests</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Matrix Proteins (immunology)</term>
<term>Viral Structural Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (biosynthèse)</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps monoclonaux (biosynthèse)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Cartographie épitopique</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (immunologie)</term>
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<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Tests de neutralisation</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Membrane Glycoproteins</term>
<term>Nucleocapsid Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
<term>Viral Structural Proteins</term>
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<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps monoclonaux</term>
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<term>Anticorps monoclonaux</term>
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<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
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<term>Antibody Specificity</term>
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<term>Chlorocebus aethiops</term>
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<front><div type="abstract" xml:lang="en">Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.</div>
</front>
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<DateCompleted><Year>2005</Year>
<Month>09</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0166-0934</ISSN>
<JournalIssue CitedMedium="Print"><Volume>128</Volume>
<Issue>1-2</Issue>
<PubDate><Year>2005</Year>
<Month>Sep</Month>
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<Title>Journal of virological methods</Title>
<ISOAbbreviation>J. Virol. Methods</ISOAbbreviation>
</Journal>
<ArticleTitle>Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins.</ArticleTitle>
<Pagination><MedlinePgn>21-8</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tripp</LastName>
<ForeName>Ralph A</ForeName>
<Initials>RA</Initials>
<AffiliationInfo><Affiliation>National Centers for Infectious Diseases, Division of Viral and Rickettsial Diseases, Respiratory and Enteric Virus Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-09, Atlanta, GA 30333, USA.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Haynes</LastName>
<ForeName>Lia M</ForeName>
<Initials>LM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Moore</LastName>
<ForeName>Deborah</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Anderson</LastName>
<ForeName>Barbara</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Tamin</LastName>
<ForeName>Azaibi</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Harcourt</LastName>
<ForeName>Brian H</ForeName>
<Initials>BH</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jones</LastName>
<ForeName>Les P</ForeName>
<Initials>LP</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yilla</LastName>
<ForeName>Mamadi</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Babcock</LastName>
<ForeName>Gregory J</ForeName>
<Initials>GJ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Greenough</LastName>
<ForeName>Thomas</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ambrosino</LastName>
<ForeName>Donna M</ForeName>
<Initials>DM</Initials>
</Author>
<Author ValidYN="Y"><LastName>Alvarez</LastName>
<ForeName>Rene</ForeName>
<Initials>R</Initials>
</Author>
<Author ValidYN="Y"><LastName>Callaway</LastName>
<ForeName>Justin</ForeName>
<Initials>J</Initials>
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<Author ValidYN="Y"><LastName>Cavitt</LastName>
<ForeName>Sheana</ForeName>
<Initials>S</Initials>
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<Author ValidYN="Y"><LastName>Harcourt</LastName>
<ForeName>Jennifer L</ForeName>
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