Pathology of guinea pigs experimentally infected with a novel reovirus and coronavirus isolated from SARS patients.
Identifieur interne : 002588 ( PubMed/Checkpoint ); précédent : 002587; suivant : 002589Pathology of guinea pigs experimentally infected with a novel reovirus and coronavirus isolated from SARS patients.
Auteurs : Licheng Liang [République populaire de Chine] ; Cheng He ; Ming Lei ; Shaowen Li ; Yongxin Hao ; Hong Zhu ; Qing DuanSource :
- DNA and cell biology [ 1044-5498 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (sang), Cochons d'Inde, Coronavirus (immunologie), Coronavirus (isolement et purification), Foie (anatomopathologie), Humains, Lignée cellulaire tumorale, Modèles animaux de maladie humaine, Noeuds lymphatiques (anatomopathologie), Nécrose, Poumon (anatomopathologie), Reoviridae (immunologie), Reoviridae (isolement et purification), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie).
- MESH :
- anatomopathologie : Foie, Noeuds lymphatiques, Poumon, Syndrome respiratoire aigu sévère.
- immunologie : Coronavirus, Reoviridae, Syndrome respiratoire aigu sévère.
- isolement et purification : Coronavirus, Reoviridae.
- sang : Anticorps antiviraux.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cochons d'Inde, Humains, Lignée cellulaire tumorale, Modèles animaux de maladie humaine, Nécrose.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Cell Line, Tumor, Coronavirus (immunology), Coronavirus (isolation & purification), Disease Models, Animal, Guinea Pigs, Humans, Liver (pathology), Lung (pathology), Lymph Nodes (pathology), Necrosis, Reoviridae (immunology), Reoviridae (isolation & purification), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , blood : Antibodies, Viral.
- immunology : Coronavirus, Reoviridae, Severe Acute Respiratory Syndrome.
- isolation & purification : Coronavirus, Reoviridae.
- pathology : Liver, Lung, Lymph Nodes, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Tumor, Disease Models, Animal, Guinea Pigs, Humans, Necrosis.
Abstract
Guinea pigs were inoculated with a reovirus (ReoV) and coronavirus (SARS-CoV) isolated from SARS patients to determine their potential role in the etiology of SARS. Animals infected with ReoV died between day 22 and day 30 postinoculation (PI) while 70% of the animals inoculated with ReoV and SARS-CoV died between day 4 to day 7 PI. The titer of neutralizing antibodies against ReoV and SARS-CoV ranged from 80 to 160 when the animals were inoculated with the two viruses, respectively, while the titer of the antibodies was just below 10 in coinfections. The animal inoculated with ReoV developed diffuse alveolar damage similar to the exudative and leakage inflammation found in SARS patients, and was characterized by diffuse hemorrhage, fibroid exudation, hyaline membrane formation, and type II pneumocytes hyperplasia in alveolar interstitia. The pulmonary epithelial necrosis, excoriation, and early fibrosis of pulmonary tissue were only observed in ReoV-SARS-CoV groups and in SARS-CoV/ReoV groups. Other typical pathological changes included hemorrhagic necrosis in lymph nodes and spleen and hydropic degeneration in the liver. On the contrary, guinea pigs infected with SARS-CoV only developed interstitial pneumonitis. Our experiment demonstrate that ReoV might be one of the primary causes of SARS, since simultaneous coinfection can duplicate the typical pathological changes similar to that of SARS patients. This guinea pig model may provide a useful animal model for SARS.
DOI: 10.1089/dna.2005.24.485
PubMed: 16101345
Affiliations:
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humaine</term><term>Noeuds lymphatiques (anatomopathologie)</term><term>Nécrose</term><term>Poumon (anatomopathologie)</term><term>Reoviridae (immunologie)</term><term>Reoviridae (isolement et purification)</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Foie</term><term>Noeuds lymphatiques</term><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus</term><term>Reoviridae</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus</term><term>Reoviridae</term><term>Severe 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Pigs</term><term>Humans</term><term>Necrosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cochons d'Inde</term><term>Humains</term><term>Lignée cellulaire tumorale</term><term>Modèles animaux de maladie humaine</term><term>Nécrose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Guinea pigs were inoculated with a reovirus (ReoV) and coronavirus (SARS-CoV) isolated from SARS patients to determine their potential role in the etiology of SARS. Animals infected with ReoV died between day 22 and day 30 postinoculation (PI) while 70% of the animals inoculated with ReoV and SARS-CoV died between day 4 to day 7 PI. The titer of neutralizing antibodies against ReoV and SARS-CoV ranged from 80 to 160 when the animals were inoculated with the two viruses, respectively, while the titer of the antibodies was just below 10 in coinfections. The animal inoculated with ReoV developed diffuse alveolar damage similar to the exudative and leakage inflammation found in SARS patients, and was characterized by diffuse hemorrhage, fibroid exudation, hyaline membrane formation, and type II pneumocytes hyperplasia in alveolar interstitia. The pulmonary epithelial necrosis, excoriation, and early fibrosis of pulmonary tissue were only observed in ReoV-SARS-CoV groups and in SARS-CoV/ReoV groups. Other typical pathological changes included hemorrhagic necrosis in lymph nodes and spleen and hydropic degeneration in the liver. On the contrary, guinea pigs infected with SARS-CoV only developed interstitial pneumonitis. Our experiment demonstrate that ReoV might be one of the primary causes of SARS, since simultaneous coinfection can duplicate the typical pathological changes similar to that of SARS patients. This guinea pig model may provide a useful animal model for SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16101345</PMID><DateCompleted><Year>2005</Year><Month>09</Month><Day>29</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1044-5498</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>8</Issue><PubDate><Year>2005</Year><Month>Aug</Month></PubDate></JournalIssue><Title>DNA and cell biology</Title><ISOAbbreviation>DNA Cell Biol.</ISOAbbreviation></Journal><ArticleTitle>Pathology of guinea pigs experimentally infected with a novel reovirus and coronavirus isolated from SARS patients.</ArticleTitle><Pagination><MedlinePgn>485-90</MedlinePgn></Pagination><Abstract><AbstractText>Guinea pigs were inoculated with a reovirus (ReoV) and coronavirus (SARS-CoV) isolated from SARS patients to determine their potential role in the etiology of SARS. Animals infected with ReoV died between day 22 and day 30 postinoculation (PI) while 70% of the animals inoculated with ReoV and SARS-CoV died between day 4 to day 7 PI. The titer of neutralizing antibodies against ReoV and SARS-CoV ranged from 80 to 160 when the animals were inoculated with the two viruses, respectively, while the titer of the antibodies was just below 10 in coinfections. The animal inoculated with ReoV developed diffuse alveolar damage similar to the exudative and leakage inflammation found in SARS patients, and was characterized by diffuse hemorrhage, fibroid exudation, hyaline membrane formation, and type II pneumocytes hyperplasia in alveolar interstitia. The pulmonary epithelial necrosis, excoriation, and early fibrosis of pulmonary tissue were only observed in ReoV-SARS-CoV groups and in SARS-CoV/ReoV groups. Other typical pathological changes included hemorrhagic necrosis in lymph nodes and spleen and hydropic degeneration in the liver. On the contrary, guinea pigs infected with SARS-CoV only developed interstitial pneumonitis. Our experiment demonstrate that ReoV might be one of the primary causes of SARS, since simultaneous coinfection can duplicate the typical pathological changes similar to that of SARS patients. This guinea pig model may provide a useful animal model for SARS.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Licheng</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Laboratory Animal Institute, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Cheng</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Lei</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Shaowen</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Hao</LastName><ForeName>Yongxin</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Hong</ForeName><Initials>H</Initials></Author><Author 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first="Cheng" last="He">Cheng He</name><name sortKey="Lei, Ming" sort="Lei, Ming" uniqKey="Lei M" first="Ming" last="Lei">Ming Lei</name><name sortKey="Li, Shaowen" sort="Li, Shaowen" uniqKey="Li S" first="Shaowen" last="Li">Shaowen Li</name><name sortKey="Zhu, Hong" sort="Zhu, Hong" uniqKey="Zhu H" first="Hong" last="Zhu">Hong Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liang, Licheng" sort="Liang, Licheng" uniqKey="Liang L" first="Licheng" last="Liang">Licheng Liang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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