Potent and specific inhibition of SARS-CoV antigen expression by RNA interference.
Identifieur interne : 002577 ( PubMed/Checkpoint ); précédent : 002576; suivant : 002578Potent and specific inhibition of SARS-CoV antigen expression by RNA interference.
Auteurs : Peng Tao [République populaire de Chine] ; Jun Zhang ; Ni Tang ; Bing-Qiang Zhang ; Tong-Chuan He ; Ai-Long HuangSource :
- Chinese medical journal [ 0366-6999 ] ; 2005.
Descripteurs français
- KwdFr :
- Antigènes viraux (génétique), Cellules cultivées, Humains, Interférence par ARN, Protéines nucléocapside (antagonistes et inhibiteurs), Protéines nucléocapside (génétique), Protéines à fluorescence verte (génétique), Syndrome respiratoire aigu sévère (), Vecteurs génétiques, Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- antagonistes et inhibiteurs : Protéines nucléocapside.
- génétique : Antigènes viraux, Protéines nucléocapside, Protéines à fluorescence verte, Virus du SRAS.
- immunologie : Virus du SRAS.
- Cellules cultivées, Humains, Interférence par ARN, Syndrome respiratoire aigu sévère, Vecteurs génétiques.
English descriptors
- KwdEn :
- Antigens, Viral (genetics), Cells, Cultured, Genetic Vectors, Green Fluorescent Proteins (genetics), Humans, Nucleocapsid Proteins (antagonists & inhibitors), Nucleocapsid Proteins (genetics), RNA Interference, SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (therapy).
- MESH :
- chemical , antagonists & inhibitors : Nucleocapsid Proteins.
- chemical , genetics : Antigens, Viral, Green Fluorescent Proteins, Nucleocapsid Proteins.
- genetics : SARS Virus.
- immunology : SARS Virus.
- therapy : Severe Acute Respiratory Syndrome.
- Cells, Cultured, Genetic Vectors, Humans, RNA Interference.
Abstract
Severe acute respiratory syndrome (SARS) is an infectious disease caused by SARS-CoV. There are no effective antiviral drugs for SARS although the epidemic of SARS was controlled. The aim of this study was to develop an RNAi (RNA interference) approach that specifically targeted the N gene sequence of severe acute respiratory syndrome associated coronavirus (SARS-CoV) by synthesizing short hairpin RNA (shRNA) in vivo, and to assess the inhibitory effect of this shRNA on SARS-CoV N antigen expression.
PubMed: 15899131
Affiliations:
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uniqKey="Zhang B" first="Bing-Qiang" last="Zhang">Bing-Qiang Zhang</name></author><author><name sortKey="He, Tong Chuan" sort="He, Tong Chuan" uniqKey="He T" first="Tong-Chuan" last="He">Tong-Chuan He</name></author><author><name sortKey="Huang, Ai Long" sort="Huang, Ai Long" uniqKey="Huang A" first="Ai-Long" last="Huang">Ai-Long Huang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15899131</idno><idno type="pmid">15899131</idno><idno type="wicri:Area/PubMed/Corpus">002731</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002731</idno><idno type="wicri:Area/PubMed/Curation">002731</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002731</idno><idno type="wicri:Area/PubMed/Checkpoint">002577</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002577</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Potent and specific inhibition of SARS-CoV antigen expression by RNA interference.</title><author><name sortKey="Tao, Peng" sort="Tao, Peng" uniqKey="Tao P" first="Peng" last="Tao">Peng Tao</name><affiliation wicri:level="1"><nlm:affiliation>The Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010</wicri:regionArea><wicri:noRegion>Chongqing 400010</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Jun" sort="Zhang, Jun" uniqKey="Zhang J" first="Jun" last="Zhang">Jun Zhang</name></author><author><name sortKey="Tang, Ni" sort="Tang, Ni" uniqKey="Tang N" first="Ni" last="Tang">Ni Tang</name></author><author><name sortKey="Zhang, Bing Qiang" sort="Zhang, Bing Qiang" uniqKey="Zhang B" first="Bing-Qiang" last="Zhang">Bing-Qiang Zhang</name></author><author><name sortKey="He, Tong Chuan" sort="He, Tong Chuan" uniqKey="He T" first="Tong-Chuan" last="He">Tong-Chuan He</name></author><author><name sortKey="Huang, Ai Long" sort="Huang, Ai Long" uniqKey="Huang A" first="Ai-Long" last="Huang">Ai-Long Huang</name></author></analytic><series><title level="j">Chinese medical journal</title><idno type="ISSN">0366-6999</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Viral (genetics)</term><term>Cells, Cultured</term><term>Genetic Vectors</term><term>Green Fluorescent Proteins (genetics)</term><term>Humans</term><term>Nucleocapsid Proteins (antagonists & inhibitors)</term><term>Nucleocapsid Proteins (genetics)</term><term>RNA Interference</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (therapy)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes viraux (génétique)</term><term>Cellules cultivées</term><term>Humains</term><term>Interférence par ARN</term><term>Protéines nucléocapside (antagonistes et inhibiteurs)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines à fluorescence verte (génétique)</term><term>Syndrome respiratoire aigu sévère ()</term><term>Vecteurs génétiques</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term><term>Green Fluorescent Proteins</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>Protéines nucléocapside</term><term>Protéines à fluorescence verte</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Genetic Vectors</term><term>Humans</term><term>RNA Interference</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Humains</term><term>Interférence par ARN</term><term>Syndrome respiratoire aigu sévère</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an infectious disease caused by SARS-CoV. There are no effective antiviral drugs for SARS although the epidemic of SARS was controlled. The aim of this study was to develop an RNAi (RNA interference) approach that specifically targeted the N gene sequence of severe acute respiratory syndrome associated coronavirus (SARS-CoV) by synthesizing short hairpin RNA (shRNA) in vivo, and to assess the inhibitory effect of this shRNA on SARS-CoV N antigen expression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15899131</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>30</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0366-6999</ISSN><JournalIssue CitedMedium="Print"><Volume>118</Volume><Issue>9</Issue><PubDate><Year>2005</Year><Month>May</Month><Day>05</Day></PubDate></JournalIssue><Title>Chinese medical journal</Title><ISOAbbreviation>Chin. Med. J.</ISOAbbreviation></Journal><ArticleTitle>Potent and specific inhibition of SARS-CoV antigen expression by RNA interference.</ArticleTitle><Pagination><MedlinePgn>714-9</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Severe acute respiratory syndrome (SARS) is an infectious disease caused by SARS-CoV. There are no effective antiviral drugs for SARS although the epidemic of SARS was controlled. The aim of this study was to develop an RNAi (RNA interference) approach that specifically targeted the N gene sequence of severe acute respiratory syndrome associated coronavirus (SARS-CoV) by synthesizing short hairpin RNA (shRNA) in vivo, and to assess the inhibitory effect of this shRNA on SARS-CoV N antigen expression.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The eukaryotic expression plasmid pEGFP-C1-N, containing SARS-CoV N gene, was co-transfected into 293 cells with either the RNAi plasmid pshRNA-N or unrelated control plasmid pshRNA-HBV-C4. At 24, 48 and 72 hours post transfection, the green fluorescence was observed through a fluorescence microscope. The RNA levels of SARS-CoV N were determined by reverse transcription polymerase chain reaction (RT-PCR). The expression of Green Fluorescent Protein (GFP) and protein N were detected using Western blot.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The vector, pshRNA-N expressing shRNA which targeted the N gene of SARS-CoV, was successfully constructed. The introduction of RNAi plasmid efficiently and specifically inhibited the synthesis of protein N. RT-PCR showed that RNAs of N gene were clearly reduced when the pEGFP-C1-N was cotransfected with pshRNA-N, whereas the control vector did not exhibit inhibitory effect on N gene transcription.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Our results demonstrate that RNAi mediated silencing of SARS-CoV gene could effectively inhibit expression of SARS-CoV antigen, hence RNAi based strategy should be further explored as a more efficacious antiviral therapy of SARS-CoV infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>The Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jun</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Ni</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Bing-qiang</ForeName><Initials>BQ</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Tong-chuan</ForeName><Initials>TC</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Ai-long</ForeName><Initials>AL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Chin Med J (Engl)</MedlineTA><NlmUniqueID>7513795</NlmUniqueID><ISSNLinking>0366-6999</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="Y">RNA Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000628" MajorTopicYN="N">therapy</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>5</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>7</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>5</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15899131</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="He, Tong Chuan" sort="He, Tong Chuan" uniqKey="He T" first="Tong-Chuan" last="He">Tong-Chuan He</name><name sortKey="Huang, Ai Long" sort="Huang, Ai Long" uniqKey="Huang A" first="Ai-Long" last="Huang">Ai-Long Huang</name><name sortKey="Tang, Ni" sort="Tang, Ni" uniqKey="Tang N" first="Ni" last="Tang">Ni Tang</name><name sortKey="Zhang, Bing Qiang" sort="Zhang, Bing Qiang" uniqKey="Zhang B" first="Bing-Qiang" last="Zhang">Bing-Qiang Zhang</name><name sortKey="Zhang, Jun" sort="Zhang, Jun" uniqKey="Zhang J" first="Jun" last="Zhang">Jun Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Tao, Peng" sort="Tao, Peng" uniqKey="Tao P" first="Peng" last="Tao">Peng Tao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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