Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection.
Identifieur interne : 002556 ( PubMed/Checkpoint ); précédent : 002555; suivant : 002557Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection.
Auteurs : Xiao-Sheng Jiang [République populaire de Chine] ; Liu-Ya Tang ; Jie Dai ; Hu Zhou ; Su-Jun Li ; Qi-Chang Xia ; Jia-Rui Wu ; Rong ZengSource :
- Molecular & cellular proteomics : MCP [ 1535-9476 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Proteome.
- metabolism : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Electrophoresis, Gel, Two-Dimensional, Isotope Labeling, Molecular Sequence Data, Spectrometry, Mass, Electrospray Ionization, Vero Cells.
Abstract
We present the first proteomic analysis on the cellular response to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. The differential proteomes of Vero E6 cells with and without infection of the SARS-CoV were resolved and quantitated with two-dimensional differential gel electrophoresis followed by ESI-MS/MS identification. Moreover isotope-coded affinity tag technology coupled with two-dimensional LC-MS/MS were also applied to the differential proteins of infected cells. By combining these two complementary strategies, 355 unique proteins were identified and quantitated with 186 of them differentially expressed (at least 1.5-fold quantitative alteration) between infected and uninfected Vero E6 cells. The implication for cellular responses to virus infection was analyzed in depth according to the proteomic results. Thus, the present work provides large scale protein-related information to investigate the mechanism of SARS-CoV infection and pathogenesis.
DOI: 10.1074/mcp.M400112-MCP200
PubMed: 15784933
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:15784933Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection.</title><author><name sortKey="Jiang, Xiao Sheng" sort="Jiang, Xiao Sheng" uniqKey="Jiang X" first="Xiao-Sheng" last="Jiang">Xiao-Sheng Jiang</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031</wicri:regionArea><wicri:noRegion>Shanghai 200031</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Liu Ya" sort="Tang, Liu Ya" uniqKey="Tang L" first="Liu-Ya" last="Tang">Liu-Ya Tang</name></author><author><name sortKey="Dai, Jie" sort="Dai, Jie" uniqKey="Dai J" first="Jie" last="Dai">Jie Dai</name></author><author><name sortKey="Zhou, Hu" sort="Zhou, Hu" uniqKey="Zhou H" first="Hu" last="Zhou">Hu Zhou</name></author><author><name sortKey="Li, Su Jun" sort="Li, Su Jun" uniqKey="Li S" first="Su-Jun" last="Li">Su-Jun Li</name></author><author><name sortKey="Xia, Qi Chang" sort="Xia, Qi Chang" uniqKey="Xia Q" first="Qi-Chang" last="Xia">Qi-Chang Xia</name></author><author><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia-Rui" last="Wu">Jia-Rui Wu</name></author><author><name sortKey="Zeng, Rong" sort="Zeng, Rong" uniqKey="Zeng R" first="Rong" last="Zeng">Rong Zeng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15784933</idno><idno type="pmid">15784933</idno><idno type="doi">10.1074/mcp.M400112-MCP200</idno><idno type="wicri:Area/PubMed/Corpus">002827</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002827</idno><idno type="wicri:Area/PubMed/Curation">002827</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002827</idno><idno type="wicri:Area/PubMed/Checkpoint">002556</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002556</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection.</title><author><name sortKey="Jiang, Xiao Sheng" sort="Jiang, Xiao Sheng" uniqKey="Jiang X" first="Xiao-Sheng" last="Jiang">Xiao-Sheng Jiang</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031</wicri:regionArea><wicri:noRegion>Shanghai 200031</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Liu Ya" sort="Tang, Liu Ya" uniqKey="Tang L" first="Liu-Ya" last="Tang">Liu-Ya Tang</name></author><author><name sortKey="Dai, Jie" sort="Dai, Jie" uniqKey="Dai J" first="Jie" last="Dai">Jie Dai</name></author><author><name sortKey="Zhou, Hu" sort="Zhou, Hu" uniqKey="Zhou H" first="Hu" last="Zhou">Hu Zhou</name></author><author><name sortKey="Li, Su Jun" sort="Li, Su Jun" uniqKey="Li S" first="Su-Jun" last="Li">Su-Jun Li</name></author><author><name sortKey="Xia, Qi Chang" sort="Xia, Qi Chang" uniqKey="Xia Q" first="Qi-Chang" last="Xia">Qi-Chang Xia</name></author><author><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia-Rui" last="Wu">Jia-Rui Wu</name></author><author><name sortKey="Zeng, Rong" sort="Zeng, Rong" uniqKey="Zeng R" first="Rong" last="Zeng">Rong Zeng</name></author></analytic><series><title level="j">Molecular & cellular proteomics : MCP</title><idno type="ISSN">1535-9476</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Isotope Labeling</term><term>Molecular Sequence Data</term><term>Proteome (metabolism)</term><term>SARS Virus (metabolism)</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Marquage isotopique</term><term>Protéome (métabolisme)</term><term>Spectrométrie de masse ESI</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (métabolisme)</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéome</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Isotope Labeling</term><term>Molecular Sequence Data</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Marquage isotopique</term><term>Spectrométrie de masse ESI</term><term>Séquence d'acides aminés</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We present the first proteomic analysis on the cellular response to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. The differential proteomes of Vero E6 cells with and without infection of the SARS-CoV were resolved and quantitated with two-dimensional differential gel electrophoresis followed by ESI-MS/MS identification. Moreover isotope-coded affinity tag technology coupled with two-dimensional LC-MS/MS were also applied to the differential proteins of infected cells. By combining these two complementary strategies, 355 unique proteins were identified and quantitated with 186 of them differentially expressed (at least 1.5-fold quantitative alteration) between infected and uninfected Vero E6 cells. The implication for cellular responses to virus infection was analyzed in depth according to the proteomic results. Thus, the present work provides large scale protein-related information to investigate the mechanism of SARS-CoV infection and pathogenesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15784933</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1535-9476</ISSN><JournalIssue CitedMedium="Print"><Volume>4</Volume><Issue>7</Issue><PubDate><Year>2005</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Molecular & cellular proteomics : MCP</Title><ISOAbbreviation>Mol. Cell Proteomics</ISOAbbreviation></Journal><ArticleTitle>Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection.</ArticleTitle><Pagination><MedlinePgn>902-13</MedlinePgn></Pagination><Abstract><AbstractText>We present the first proteomic analysis on the cellular response to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. The differential proteomes of Vero E6 cells with and without infection of the SARS-CoV were resolved and quantitated with two-dimensional differential gel electrophoresis followed by ESI-MS/MS identification. Moreover isotope-coded affinity tag technology coupled with two-dimensional LC-MS/MS were also applied to the differential proteins of infected cells. By combining these two complementary strategies, 355 unique proteins were identified and quantitated with 186 of them differentially expressed (at least 1.5-fold quantitative alteration) between infected and uninfected Vero E6 cells. The implication for cellular responses to virus infection was analyzed in depth according to the proteomic results. Thus, the present work provides large scale protein-related information to investigate the mechanism of SARS-CoV infection and pathogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Xiao-Sheng</ForeName><Initials>XS</Initials><AffiliationInfo><Affiliation>Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Liu-Ya</ForeName><Initials>LY</Initials></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Hu</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Su-Jun</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>Qi-Chang</ForeName><Initials>QC</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Jia-Rui</ForeName><Initials>JR</Initials></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Rong</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>03</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Cell Proteomics</MedlineTA><NlmUniqueID>101125647</NlmUniqueID><ISSNLinking>1535-9476</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007553" MajorTopicYN="N">Isotope Labeling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>12</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15784933</ArticleId><ArticleId IdType="pii">M400112-MCP200</ArticleId><ArticleId IdType="doi">10.1074/mcp.M400112-MCP200</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Dai, Jie" sort="Dai, Jie" uniqKey="Dai J" first="Jie" last="Dai">Jie Dai</name><name sortKey="Li, Su Jun" sort="Li, Su Jun" uniqKey="Li S" first="Su-Jun" last="Li">Su-Jun Li</name><name sortKey="Tang, Liu Ya" sort="Tang, Liu Ya" uniqKey="Tang L" first="Liu-Ya" last="Tang">Liu-Ya Tang</name><name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia-Rui" last="Wu">Jia-Rui Wu</name><name sortKey="Xia, Qi Chang" sort="Xia, Qi Chang" uniqKey="Xia Q" first="Qi-Chang" last="Xia">Qi-Chang Xia</name><name sortKey="Zeng, Rong" sort="Zeng, Rong" uniqKey="Zeng R" first="Rong" last="Zeng">Rong Zeng</name><name sortKey="Zhou, Hu" sort="Zhou, Hu" uniqKey="Zhou H" first="Hu" last="Zhou">Hu Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Jiang, Xiao Sheng" sort="Jiang, Xiao Sheng" uniqKey="Jiang X" first="Xiao-Sheng" last="Jiang">Xiao-Sheng Jiang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002556 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002556 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:15784933
|texte= Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:15784933" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |