SrasV1, PubMed, Checkpoint, bibRecord, 002544

Receptor-binding domain of SARS-Cov spike protein: soluble expression in E. coli, purification and functional characterization.

Identifieur interne : 002544 ( PubMed/Checkpoint ); précédent : 002543; suivant : 002545

Receptor-binding domain of SARS-Cov spike protein: soluble expression in E. coli, purification and functional characterization.

Auteurs : Jing Chen [République populaire de Chine] ; Lin Miao ; Jia-Ming Li ; Yan-Ying Li ; Qing-Yu Zhu ; Chang-Lin Zhou ; Hong-Qing Fang ; Hui-Peng Chen

Source :

World journal of gastroenterology [ 1007-9327 ] ; 2005.

RBID : pubmed:16273643

Descripteurs français

English descriptors

KwdEn :
- Animals, Chlorocebus aethiops, Escherichia coli, Gene Expression Regulation, Viral, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Plasmids, Protein Structure, Tertiary, SARS Virus (genetics), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), Solubility, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Vaccines (chemistry), Viral Vaccines (genetics).
MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- genetics : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Escherichia coli, Gene Expression Regulation, Viral, Plasmids, Protein Structure, Tertiary, Solubility, Spike Glycoprotein, Coronavirus, Vero Cells.

Abstract

Spike protein of coronavirus is responsible for virus binding, fusion and entry, and is a major inducer of neutralizing antibodies. This paper was to find a soluble and functional recombinant receptor-binding domain of severe acute respiratory syndrome-associated coronavirus (SARS-Cov), and to analyze its receptor binding ability.

DOI: 10.3748/wjg.v11.i39.6159
PubMed: 16273643

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002457
to stream PubMed, to step Curation: 002457

Links to Exploration step

pubmed:16273643

Le document en format XML

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<front><div type="abstract" xml:lang="en">Spike protein of coronavirus is responsible for virus binding, fusion and entry, and is a major inducer of neutralizing antibodies. This paper was to find a soluble and functional recombinant receptor-binding domain of severe acute respiratory syndrome-associated coronavirus (SARS-Cov), and to analyze its receptor binding ability.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16273643</PMID>
<DateCompleted><Year>2006</Year>
<Month>02</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-9327</ISSN>
<JournalIssue CitedMedium="Print"><Volume>11</Volume>
<Issue>39</Issue>
<PubDate><Year>2005</Year>
<Month>Oct</Month>
<Day>21</Day>
</PubDate>
</JournalIssue>
<Title>World journal of gastroenterology</Title>
<ISOAbbreviation>World J. Gastroenterol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Receptor-binding domain of SARS-Cov spike protein: soluble expression in E. coli, purification and functional characterization.</ArticleTitle>
<Pagination><MedlinePgn>6159-64</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">Spike protein of coronavirus is responsible for virus binding, fusion and entry, and is a major inducer of neutralizing antibodies. This paper was to find a soluble and functional recombinant receptor-binding domain of severe acute respiratory syndrome-associated coronavirus (SARS-Cov), and to analyze its receptor binding ability.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Three fusion tags (glutathione S-transferase, GST; thioredoxin, Trx; maltose-binding protein, MBP), which preferably contributes to increasing solubility and to facilitating the proper folding of heteroprotein, were used to acquire the soluble and functional expression of RBD protein in Escherichia coli (BL21(DE3) and Rosetta-gamiB(DE3) strains). The receptor binding ability of the purified soluble RBD protein was then detected by ELISA and flow cytometry assay.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">RBD of SARS-Cov spike protein was expressed as inclusion body when fused as TrxA tag form in both BL21 (DE3) and Rosetta-gamiB (DE3) under many different cultures and induction conditions. And there was no visible expression band on SDS-PAGE when RBD was expressed as MBP tagged form. Only GST tagged RBD was soluble expressed in BL21(DE3), and the protein was purified by AKTA Prime Chromatography system. The ELISA data showed that GST/RBD antigen had positive reaction with anti-RBD mouse monoclonal antibody 1A5. Further flow cytometry assay demonstrated the high efficiency of RBD's binding ability to ACE2 (angiotensin-converting enzyme 2) positive Vero E6 cell. And ACE2 was proved as a cellular receptor that meditated an initial-affinity interaction with SARS-Cov spike protein. The geometrical mean of GST and GST/RBD binding to Vero E6 cells were 77.08 and 352.73 respectively.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">In this paper, we get sufficient soluble N terminal GST tagged RBD protein expressed in E.coli BL21(DE3); data from ELISA and flow cytometry assay demonstrate that the recombinant protein is functional and binding to ACE2 positive Vero E6 cell efficiently. And the recombinant RBD derived from E.coli can be used to developing subunit vaccine to block S protein binding with receptor and to neutralizing SARS-Cov infection.</AbstractText>
</Abstract>
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<ForeName>Jing</ForeName>
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<AffiliationInfo><Affiliation>Institute of Biotechnology, Academy of Military Medical Science, 20 Dongda Street, Fengtai District, Beijing 100071, China.</Affiliation>
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<ForeName>Lin</ForeName>
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<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Jia-Ming</ForeName>
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<Author ValidYN="Y"><LastName>Li</LastName>
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<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Chang-Lin</ForeName>
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<ForeName>Hong-Qing</ForeName>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance>
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<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4240-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15010527</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2003 Dec 26;312(4):1159-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14651994</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8455-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15150417</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Feb 20;314(4):931-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14751221</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Mar 19;315(4):1134-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14985131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Apr 1;428(6982):561-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15024391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Med Virol. 2004 Jul;73(3):338-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15170626</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1948-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12748314</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>World J Gastroenterol. 2003 Aug;9(8):1635-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12918094</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2004 Feb;4(2):492-504</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14760722</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Nov 12;324(2):773-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15474494</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Jun 11;318(4):833-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15147946</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 Jul 10;349(2):187-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12853594</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2004 Mar 20;363(9413):938-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15043961</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6641-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15096611</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2536-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14983044</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2003 Oct 10;310(1):78-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14511651</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Jan 23;313(4):938-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14706633</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 Dec 6;362(9399):1895-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14667748</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2003 May 15;423(6937):240</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12748632</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1986-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12682352</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Mar 5;315(2):439-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14766227</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation>
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<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15748-53</Citation>
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<Reference><Citation>J Biol Chem. 2004 Jan 30;279(5):3197-201</Citation>
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<Reference><Citation>Emerg Infect Dis. 2004 Feb;10(2):176-8</Citation>
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<name sortKey="Li, Jia Ming" sort="Li, Jia Ming" uniqKey="Li J" first="Jia-Ming" last="Li">Jia-Ming Li</name>
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Receptor-binding domain of SARS-Cov spike protein: soluble expression in E. coli, purification and functional characterization.

Receptor-binding domain of SARS-Cov spike protein: soluble expression in E. coli, purification and functional characterization.

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