[A preliminary study of the structure prediction and expression of SARS-CoV spike protein].
Identifieur interne : 002391 ( PubMed/Checkpoint ); précédent : 002390; suivant : 002392[A preliminary study of the structure prediction and expression of SARS-CoV spike protein].
Auteurs : Mei-Ling Sun [République populaire de Chine] ; Jin-Hua Piao ; Chang-Cheng Yin ; Gong-She Yang ; Hua-Liang HuangSource :
- Yi chuan = Hereditas [ 0253-9772 ] ; 2005.
Descripteurs français
- KwdFr :
- Escherichia coli (génétique), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Pichia (génétique), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines recombinantes (), Protéines recombinantes (métabolisme), Régulation de l'expression des gènes viraux, Signaux de triage des protéines (génétique), Structure tertiaire des protéines, Technique de Western, Vecteurs génétiques (génétique), Virus du SRAS (génétique), Virus du SRAS (métabolisme), Épitopes (), Épitopes (génétique), Épitopes (métabolisme).
- MESH :
- génétique : Escherichia coli, Glycoprotéines membranaires, Pichia, Protéines de l'enveloppe virale, Signaux de triage des protéines, Vecteurs génétiques, Virus du SRAS, Épitopes.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Virus du SRAS, Épitopes.
- Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Régulation de l'expression des gènes viraux, Structure tertiaire des protéines, Technique de Western, Épitopes.
English descriptors
- KwdEn :
- Blotting, Western, Epitopes (chemistry), Epitopes (genetics), Epitopes (metabolism), Escherichia coli (genetics), Gene Expression Regulation, Viral, Genetic Vectors (genetics), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Pichia (genetics), Protein Sorting Signals (genetics), Protein Structure, Tertiary, Recombinant Proteins (chemistry), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Epitopes, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , genetics : Epitopes, Membrane Glycoproteins, Protein Sorting Signals, Viral Envelope Proteins.
- chemical , metabolism : Epitopes, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- genetics : Escherichia coli, Genetic Vectors, Pichia, SARS Virus.
- metabolism : SARS Virus.
- Blotting, Western, Gene Expression Regulation, Viral, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus.
Abstract
In this study, the encoding sequences of SARS-CoV spike protein were analyzed by bioinformatics methods, the structural characteristics and functions were forecasted based on available data. It suggests that the fragment of spike glycoprotein (S401-659) may be crucial for viral attachment and may be a major immunodominant epitope. Then the fragment was amplified and subcloned into expression vector pET28a(+) and pPIC9K. These two plasmids pET28a(+)-S and pPIC9K-S were transformed to E.coli strain BL21(DE3)-star and Pichia pastoris, respectively. SDS-PAGE and Western blot analysis showed that the recombinant protein was successfully expressed. The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.
PubMed: 16120590
Affiliations:
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pubmed:16120590Le document en format XML
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<author><name sortKey="Sun, Mei Ling" sort="Sun, Mei Ling" uniqKey="Sun M" first="Mei-Ling" last="Sun">Mei-Ling Sun</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science and Technology , Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi, 712100, China. lisasun78@yahoo.com.cn</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science and Technology , Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi, 712100</wicri:regionArea>
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<author><name sortKey="Yin, Chang Cheng" sort="Yin, Chang Cheng" uniqKey="Yin C" first="Chang-Cheng" last="Yin">Chang-Cheng Yin</name>
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<term>Escherichia coli (genetics)</term>
<term>Gene Expression Regulation, Viral</term>
<term>Genetic Vectors (genetics)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Pichia (genetics)</term>
<term>Protein Sorting Signals (genetics)</term>
<term>Protein Structure, Tertiary</term>
<term>Recombinant Proteins (chemistry)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
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<term>Viral Envelope Proteins (metabolism)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Escherichia coli (génétique)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
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<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Pichia (génétique)</term>
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<term>Protein Sorting Signals</term>
<term>Viral Envelope Proteins</term>
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</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
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<term>Protéines recombinantes</term>
<term>Virus du SRAS</term>
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<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
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<front><div type="abstract" xml:lang="en">In this study, the encoding sequences of SARS-CoV spike protein were analyzed by bioinformatics methods, the structural characteristics and functions were forecasted based on available data. It suggests that the fragment of spike glycoprotein (S401-659) may be crucial for viral attachment and may be a major immunodominant epitope. Then the fragment was amplified and subcloned into expression vector pET28a(+) and pPIC9K. These two plasmids pET28a(+)-S and pPIC9K-S were transformed to E.coli strain BL21(DE3)-star and Pichia pastoris, respectively. SDS-PAGE and Western blot analysis showed that the recombinant protein was successfully expressed. The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.</div>
</front>
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<Abstract><AbstractText>In this study, the encoding sequences of SARS-CoV spike protein were analyzed by bioinformatics methods, the structural characteristics and functions were forecasted based on available data. It suggests that the fragment of spike glycoprotein (S401-659) may be crucial for viral attachment and may be a major immunodominant epitope. Then the fragment was amplified and subcloned into expression vector pET28a(+) and pPIC9K. These two plasmids pET28a(+)-S and pPIC9K-S were transformed to E.coli strain BL21(DE3)-star and Pichia pastoris, respectively. SDS-PAGE and Western blot analysis showed that the recombinant protein was successfully expressed. The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.</AbstractText>
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<name sortKey="Piao, Jin Hua" sort="Piao, Jin Hua" uniqKey="Piao J" first="Jin-Hua" last="Piao">Jin-Hua Piao</name>
<name sortKey="Yang, Gong She" sort="Yang, Gong She" uniqKey="Yang G" first="Gong-She" last="Yang">Gong-She Yang</name>
<name sortKey="Yin, Chang Cheng" sort="Yin, Chang Cheng" uniqKey="Yin C" first="Chang-Cheng" last="Yin">Chang-Cheng Yin</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Sun, Mei Ling" sort="Sun, Mei Ling" uniqKey="Sun M" first="Mei-Ling" last="Sun">Mei-Ling Sun</name>
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