SrasV1, PubMed, Checkpoint, bibRecord, 002391

[A preliminary study of the structure prediction and expression of SARS-CoV spike protein].

Identifieur interne : 002391 ( PubMed/Checkpoint ); précédent : 002390; suivant : 002392

[A preliminary study of the structure prediction and expression of SARS-CoV spike protein].

Auteurs : Mei-Ling Sun [République populaire de Chine] ; Jin-Hua Piao ; Chang-Cheng Yin ; Gong-She Yang ; Hua-Liang Huang

Source :

Yi chuan = Hereditas [ 0253-9772 ] ; 2005.

RBID : pubmed:16120590

Descripteurs français

English descriptors

KwdEn :
- Blotting, Western, Epitopes (chemistry), Epitopes (genetics), Epitopes (metabolism), Escherichia coli (genetics), Gene Expression Regulation, Viral, Genetic Vectors (genetics), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Pichia (genetics), Protein Sorting Signals (genetics), Protein Structure, Tertiary, Recombinant Proteins (chemistry), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
MESH :
- chemical , chemistry : Epitopes, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , genetics : Epitopes, Membrane Glycoproteins, Protein Sorting Signals, Viral Envelope Proteins.
- chemical , metabolism : Epitopes, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- genetics : Escherichia coli, Genetic Vectors, Pichia, SARS Virus.
- metabolism : SARS Virus.
- Blotting, Western, Gene Expression Regulation, Viral, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus.

Abstract

In this study, the encoding sequences of SARS-CoV spike protein were analyzed by bioinformatics methods, the structural characteristics and functions were forecasted based on available data. It suggests that the fragment of spike glycoprotein (S401-659) may be crucial for viral attachment and may be a major immunodominant epitope. Then the fragment was amplified and subcloned into expression vector pET28a(+) and pPIC9K. These two plasmids pET28a(+)-S and pPIC9K-S were transformed to E.coli strain BL21(DE3)-star and Pichia pastoris, respectively. SDS-PAGE and Western blot analysis showed that the recombinant protein was successfully expressed. The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.

PubMed: 16120590

Affiliations:

République populaire de Chine

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pubmed:16120590

Le document en format XML

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<author><name sortKey="Sun, Mei Ling" sort="Sun, Mei Ling" uniqKey="Sun M" first="Mei-Ling" last="Sun">Mei-Ling Sun</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science and Technology , Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi, 712100, China. lisasun78@yahoo.com.cn</nlm:affiliation>
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<author><name sortKey="Yin, Chang Cheng" sort="Yin, Chang Cheng" uniqKey="Yin C" first="Chang-Cheng" last="Yin">Chang-Cheng Yin</name>
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<author><name sortKey="Yang, Gong She" sort="Yang, Gong She" uniqKey="Yang G" first="Gong-She" last="Yang">Gong-She Yang</name>
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<front><div type="abstract" xml:lang="en">In this study, the encoding sequences of SARS-CoV spike protein were analyzed by bioinformatics methods, the structural characteristics and functions were forecasted based on available data. It suggests that the fragment of spike glycoprotein (S401-659) may be crucial for viral attachment and may be a major immunodominant epitope. Then the fragment was amplified and subcloned into expression vector pET28a(+) and pPIC9K. These two plasmids pET28a(+)-S and pPIC9K-S were transformed to E.coli strain BL21(DE3)-star and Pichia pastoris, respectively. SDS-PAGE and Western blot analysis showed that the recombinant protein was successfully expressed. The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.</div>
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<Abstract><AbstractText>In this study, the encoding sequences of SARS-CoV spike protein were analyzed by bioinformatics methods, the structural characteristics and functions were forecasted based on available data. It suggests that the fragment of spike glycoprotein (S401-659) may be crucial for viral attachment and may be a major immunodominant epitope. Then the fragment was amplified and subcloned into expression vector pET28a(+) and pPIC9K. These two plasmids pET28a(+)-S and pPIC9K-S were transformed to E.coli strain BL21(DE3)-star and Pichia pastoris, respectively. SDS-PAGE and Western blot analysis showed that the recombinant protein was successfully expressed. The denatured inclusion bodies were purified with Ni-NTA chelate agarose and its purity can reach 90%.</AbstractText>
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[A preliminary study of the structure prediction and expression of SARS-CoV spike protein].

[A preliminary study of the structure prediction and expression of SARS-CoV spike protein].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki