[Expression of SARS spike gene in Shizomycete pombe].
Identifieur interne : 002366 ( PubMed/Checkpoint ); précédent : 002365; suivant : 002367[Expression of SARS spike gene in Shizomycete pombe].
Auteurs : Zhao-Xia Wu [République populaire de Chine] ; Wen-Ling Zheng ; Bao Zhang ; Yong-Xia Shi ; Wen-LiSource :
- Sheng wu gong cheng xue bao = Chinese journal of biotechnology [ 1000-3061 ] ; 2005.
Descripteurs français
- KwdFr :
- Clonage moléculaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (génétique), Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines recombinantes (biosynthèse), Protéines recombinantes (génétique), Schizosaccharomyces (génétique), Schizosaccharomyces (métabolisme), Virus du SRAS (génétique), Électroporation.
- MESH :
- biosynthèse : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Schizosaccharomyces, Virus du SRAS.
- métabolisme : Schizosaccharomyces.
- Clonage moléculaire, Glycoprotéine de spicule des coronavirus, Électroporation.
English descriptors
- KwdEn :
- Cloning, Molecular, Electroporation, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), SARS Virus (genetics), Schizosaccharomyces (genetics), Schizosaccharomyces (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics).
- MESH :
- chemical , biosynthesis : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- genetics : SARS Virus, Schizosaccharomyces.
- metabolism : Schizosaccharomyces.
- Cloning, Molecular, Electroporation, Spike Glycoprotein, Coronavirus.
Abstract
The viral spike protein is the main surface antigen of the coronavirus, and it could be useful in the research of clinical diagnosis, SARS vaccine and the structure biology.According to the analysis of the main antigen of the SARS spike protein, 5 fragments of the whole spike gene were cloned, and ligated to the vector pNMT1. Through electroporation transformantion to TCP1, the recombinant S. pombe strains capable of expressing the 5 fragments were constructed. SDS-PAGE or Western blot analysis of the induced expression products demonstrated that the 5 recombinant proteins were expressed in the fission yeast respectively.
PubMed: 16176106
Affiliations:
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pubmed:16176106Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Expression of SARS spike gene in Shizomycete pombe].</title><author><name sortKey="Wu, Zhao Xia" sort="Wu, Zhao Xia" uniqKey="Wu Z" first="Zhao-Xia" last="Wu">Zhao-Xia Wu</name><affiliation wicri:level="1"><nlm:affiliation>College of Bioscience and Engineer, Southern China University of Science and Technology, GuangZhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Bioscience and Engineer, Southern China University of Science and Technology, GuangZhou 510640</wicri:regionArea><wicri:noRegion>GuangZhou 510640</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Wen Ling" sort="Zheng, Wen Ling" uniqKey="Zheng W" first="Wen-Ling" last="Zheng">Wen-Ling Zheng</name></author><author><name sortKey="Zhang, Bao" sort="Zhang, Bao" uniqKey="Zhang B" first="Bao" last="Zhang">Bao Zhang</name></author><author><name sortKey="Shi, Yong Xia" sort="Shi, Yong Xia" uniqKey="Shi Y" first="Yong-Xia" last="Shi">Yong-Xia Shi</name></author><author><name sortKey="Wen Li" sort="Wen Li" uniqKey="Wen Li" last="Wen-Li">Wen-Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16176106</idno><idno type="pmid">16176106</idno><idno type="wicri:Area/PubMed/Corpus">002530</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002530</idno><idno type="wicri:Area/PubMed/Curation">002530</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002530</idno><idno type="wicri:Area/PubMed/Checkpoint">002366</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002366</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Expression of SARS spike gene in Shizomycete pombe].</title><author><name sortKey="Wu, Zhao Xia" sort="Wu, Zhao Xia" uniqKey="Wu Z" first="Zhao-Xia" last="Wu">Zhao-Xia Wu</name><affiliation wicri:level="1"><nlm:affiliation>College of Bioscience and Engineer, Southern China University of Science and Technology, GuangZhou 510640, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Bioscience and Engineer, Southern China University of Science and Technology, GuangZhou 510640</wicri:regionArea><wicri:noRegion>GuangZhou 510640</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Wen Ling" sort="Zheng, Wen Ling" uniqKey="Zheng W" first="Wen-Ling" last="Zheng">Wen-Ling Zheng</name></author><author><name sortKey="Zhang, Bao" sort="Zhang, Bao" uniqKey="Zhang B" first="Bao" last="Zhang">Bao Zhang</name></author><author><name sortKey="Shi, Yong Xia" sort="Shi, Yong Xia" uniqKey="Shi Y" first="Yong-Xia" last="Shi">Yong-Xia Shi</name></author><author><name sortKey="Wen Li" sort="Wen Li" uniqKey="Wen Li" last="Wen-Li">Wen-Li</name></author></analytic><series><title level="j">Sheng wu gong cheng xue bao = Chinese journal of biotechnology</title><idno type="ISSN">1000-3061</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cloning, Molecular</term><term>Electroporation</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (genetics)</term><term>SARS Virus (genetics)</term><term>Schizosaccharomyces (genetics)</term><term>Schizosaccharomyces (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Clonage moléculaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (génétique)</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (génétique)</term><term>Schizosaccharomyces (génétique)</term><term>Schizosaccharomyces (métabolisme)</term><term>Virus du SRAS (génétique)</term><term>Électroporation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Schizosaccharomyces</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Schizosaccharomyces</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Schizosaccharomyces</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Schizosaccharomyces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Electroporation</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Glycoprotéine de spicule des 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Through electroporation transformantion to TCP1, the recombinant S. pombe strains capable of expressing the 5 fragments were constructed. SDS-PAGE or Western blot analysis of the induced expression products demonstrated that the 5 recombinant proteins were expressed in the fission yeast respectively.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16176106</PMID><DateCompleted><Year>2009</Year><Month>07</Month><Day>22</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-3061</ISSN><JournalIssue CitedMedium="Print"><Volume>21</Volume><Issue>4</Issue><PubDate><Year>2005</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Sheng wu gong cheng xue bao = Chinese journal of biotechnology</Title><ISOAbbreviation>Sheng Wu Gong Cheng Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Expression of SARS spike gene in Shizomycete pombe].</ArticleTitle><Pagination><MedlinePgn>638-41</MedlinePgn></Pagination><Abstract><AbstractText>The viral spike protein is the main surface antigen of the coronavirus, and it could be useful in the research of clinical diagnosis, SARS vaccine and the structure biology.According to the analysis of the main antigen of the SARS spike protein, 5 fragments of the whole spike gene were cloned, and ligated to the vector pNMT1. Through electroporation transformantion to TCP1, the recombinant S. pombe strains capable of expressing the 5 fragments were constructed. 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