[Preparation and identification of monoclonal antibodies against SARS-CoV putative protein X4].
Identifieur interne : 002358 ( PubMed/Checkpoint ); précédent : 002357; suivant : 002359[Preparation and identification of monoclonal antibodies against SARS-CoV putative protein X4].
Auteurs : Xin Luo [République populaire de Chine] ; Ting Zhang ; Da-Long Ma ; Ying-Yu ChenSource :
- Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences [ 1671-167X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (biosynthèse), Anticorps antiviraux (immunologie), Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Cadres ouverts de lecture (génétique), Femelle, Hybridomes, Protéines virales (génétique), Protéines virales (immunologie), Protéines virales (métabolisme), Souris, Souris de lignée BALB C, Spécificité des anticorps (immunologie), Technique d'immunofluorescence, Technique de Western, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (métabolisme).
- MESH :
- biosynthèse : Anticorps antiviraux, Anticorps monoclonaux.
- génétique : Cadres ouverts de lecture, Protéines virales, Virus du SRAS.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Protéines virales, Spécificité des anticorps, Virus du SRAS.
- métabolisme : Protéines virales, Virus du SRAS.
- Animaux, Femelle, Hybridomes, Souris, Souris de lignée BALB C, Technique d'immunofluorescence, Technique de Western.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibodies, Viral (biosynthesis), Antibodies, Viral (immunology), Antibody Specificity (immunology), Blotting, Western, Female, Fluorescent Antibody Technique, Hybridomas, Mice, Mice, Inbred BALB C, Open Reading Frames (genetics), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (metabolism), Viral Proteins (genetics), Viral Proteins (immunology), Viral Proteins (metabolism).
- MESH :
- chemical , biosynthesis : Antibodies, Monoclonal, Antibodies, Viral.
- chemical , genetics : Viral Proteins.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Viral Proteins.
- genetics : Open Reading Frames, SARS Virus.
- immunology : Antibody Specificity, SARS Virus.
- metabolism : SARS Virus, Viral Proteins.
- Animals, Blotting, Western, Female, Fluorescent Antibody Technique, Hybridomas, Mice, Mice, Inbred BALB C.
Abstract
To obtain monoclonal antibodies against putative protein X4 of SARS-CoV for further study of the structure and function of protein X4.
PubMed: 16224518
Affiliations:
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pubmed:16224518Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Preparation and identification of monoclonal antibodies against SARS-CoV putative protein X4].</title><author><name sortKey="Luo, Xin" sort="Luo, Xin" uniqKey="Luo X" first="Xin" last="Luo">Xin Luo</name><affiliation wicri:level="4"><nlm:affiliation>Center for Human Disease Genomics, Peking University, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center for Human Disease Genomics, Peking University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name></author><author><name sortKey="Ma, Da Long" sort="Ma, Da Long" uniqKey="Ma D" first="Da-Long" last="Ma">Da-Long Ma</name></author><author><name sortKey="Chen, Ying Yu" sort="Chen, Ying Yu" uniqKey="Chen Y" first="Ying-Yu" last="Chen">Ying-Yu Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16224518</idno><idno type="pmid">16224518</idno><idno type="wicri:Area/PubMed/Corpus">002501</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002501</idno><idno type="wicri:Area/PubMed/Curation">002501</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002501</idno><idno type="wicri:Area/PubMed/Checkpoint">002358</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002358</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Preparation and identification of monoclonal antibodies against SARS-CoV putative protein X4].</title><author><name sortKey="Luo, Xin" sort="Luo, Xin" uniqKey="Luo X" first="Xin" last="Luo">Xin Luo</name><affiliation wicri:level="4"><nlm:affiliation>Center for Human Disease Genomics, Peking University, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center for Human Disease Genomics, Peking University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name></author><author><name sortKey="Ma, Da Long" sort="Ma, Da Long" uniqKey="Ma D" first="Da-Long" last="Ma">Da-Long Ma</name></author><author><name sortKey="Chen, Ying Yu" sort="Chen, Ying Yu" uniqKey="Chen Y" first="Ying-Yu" last="Chen">Ying-Yu Chen</name></author></analytic><series><title level="j">Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences</title><idno type="ISSN">1671-167X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibodies, Viral (immunology)</term><term>Antibody Specificity (immunology)</term><term>Blotting, Western</term><term>Female</term><term>Fluorescent Antibody Technique</term><term>Hybridomas</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Open Reading Frames (genetics)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (metabolism)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (immunology)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (biosynthèse)</term><term>Anticorps monoclonaux (immunologie)</term><term>Cadres ouverts de lecture (génétique)</term><term>Femelle</term><term>Hybridomes</term><term>Protéines virales (génétique)</term><term>Protéines virales (immunologie)</term><term>Protéines virales (métabolisme)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps (immunologie)</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Open Reading Frames</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Protéines virales</term><term>Spécificité des anticorps</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Specificity</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Female</term><term>Fluorescent Antibody Technique</term><term>Hybridomas</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Hybridomes</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To obtain monoclonal antibodies against putative protein X4 of SARS-CoV for further study of the structure and function of protein X4.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16224518</PMID><DateCompleted><Year>2008</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1671-167X</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>5</Issue><PubDate><Year>2005</Year><Month>Oct</Month><Day>18</Day></PubDate></JournalIssue><Title>Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences</Title><ISOAbbreviation>Beijing Da Xue Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Preparation and identification of monoclonal antibodies against SARS-CoV putative protein X4].</ArticleTitle><Pagination><MedlinePgn>485-8</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To obtain monoclonal antibodies against putative protein X4 of SARS-CoV for further study of the structure and function of protein X4.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Balb/c mice were immunized with recombinant Protein X4, hybridoma cell lines secreting monoclonal antibodies against protein X4 were screened by regular cell fusion and subcloning approach. The specificities of these monoclonal antibodies were determined by Western blotting and Immunofluorescence assay.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Three hybridoma cell lines (8A5, 8H6 and 4A2) stable in secreting specific monoclonal antibodies were successfully obtained. They could bind specifically to protein X4 proved by Western blotting. All of them belonged to the IgG2a isotype proved by antigen mediated ELISA. Indirect Immunofluorescence assay indicated that they could specifically bind to protein X4 expressed in SARS-CoV infected Vero E6 cells.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Monoclonal antibodies of high specificity against protein X4 have been successfully prepared, which laid the foundation for the further study of protein X4.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Xin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Center for Human Disease Genomics, Peking University, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ting</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Da-long</ForeName><Initials>DL</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Ying-yu</ForeName><Initials>YY</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Beijing Da Xue Xue Bao Yi Xue Ban</MedlineTA><NlmUniqueID>101125284</NlmUniqueID><ISSNLinking>1671-167X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C515314">ORF7b protein, SARS coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000918" MajorTopicYN="N">Antibody Specificity</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006825" MajorTopicYN="N">Hybridomas</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>6</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16224518</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><noCountry><name sortKey="Chen, Ying Yu" sort="Chen, Ying Yu" uniqKey="Chen Y" first="Ying-Yu" last="Chen">Ying-Yu Chen</name><name sortKey="Ma, Da Long" sort="Ma, Da Long" uniqKey="Ma D" first="Da-Long" last="Ma">Da-Long Ma</name><name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Luo, Xin" sort="Luo, Xin" uniqKey="Luo X" first="Xin" last="Luo">Xin 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