SrasV1, PubMed, Checkpoint, bibRecord, 002333

A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.

Identifieur interne : 002333 ( PubMed/Checkpoint ); précédent : 002332; suivant : 002334

A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.

Auteurs : Yonggang Sha [République populaire de Chine] ; Yingliang Wu ; Zhijian Cao ; Xiuling Xu ; Wenlan Wu ; Dahe Jiang ; Xin Mao ; Hui Liu ; Ying Zhu ; Rui Gong ; Wenxin Li

Source :

IUBMB life [ 1521-6543 ] ; 2006.

RBID : pubmed:16916786

Descripteurs français

English descriptors

KwdEn :
- Animals, Biological Assay, COS Cells, Cell Fusion, Chlorocebus aethiops, Coculture Techniques, Escherichia coli (genetics), Fluorescent Dyes (metabolism), Glutathione Transferase (metabolism), Green Fluorescent Proteins (metabolism), Luminescent Proteins (metabolism), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Peptides (chemistry), Peptides (metabolism), Peptidyl-Dipeptidase A (metabolism), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (metabolism), Spike Glycoprotein, Coronavirus, Time Factors, Trypsin (pharmacology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
MESH :
- chemical , chemistry : Peptides.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Fluorescent Dyes, Glutathione Transferase, Green Fluorescent Proteins, Luminescent Proteins, Membrane Glycoproteins, Peptides, Peptidyl-Dipeptidase A, Recombinant Proteins, Viral Envelope Proteins.
- genetics : Escherichia coli, SARS Virus.
- metabolism : SARS Virus, Severe Acute Respiratory Syndrome.
- chemical , pharmacology : Trypsin.
- Animals, Biological Assay, COS Cells, Cell Fusion, Chlorocebus aethiops, Coculture Techniques, Spike Glycoprotein, Coronavirus, Time Factors.

Abstract

SARS-CoV spike (S) protein-mediated cell fusion is important for the viral entry mechanism and identification of SARS-CoV entry inhibitors. In order to avoid the high risks involved in handling SARS-CoV and to facilitate the study of viral fusion mechanism, we established the cell lines: SR-COS7 cells that stably express both SARS-CoV S protein and red fluorescence protein, R-COS7 cells that stably express red fluorescence protein, and AG-COS7 cells that stably express both ACE2 and green fluorescence protein, respectively. When SR-COS7 cells or R-COS7 cells were cocultured with AG-COS7 cells, syncytia with yellow fluorescence were conveniently observed after 12 h in SR-COS7 cells plus AG-COS7 cells, but not in R-COS7 cells plus AG-COS7 cells. The cell-to-cell fusion efficiency was simply determined for quantitative analysis based on the number of syncytium detected by flow cytometry. Such new cell-to-cell fusion model was further assessed by the potent HR2 peptide inhibitor, which led to the obvious decrease of the cell-to-cell fusion efficiency. The successful fusion and inhibition of cell-based binding assay shows that it can be well used for the study of SARS-CoV entry and inhibition.

DOI: 10.1080/15216540600820974
PubMed: 16916786

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002119
to stream PubMed, to step Curation: 002119

Links to Exploration step

pubmed:16916786

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.</title>
<author><name sortKey="Sha, Yonggang" sort="Sha, Yonggang" uniqKey="Sha Y" first="Yonggang" last="Sha">Yonggang Sha</name>
<affiliation wicri:level="4"><nlm:affiliation>State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan</wicri:regionArea>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
<orgName type="university">Université de Wuhan</orgName>
</affiliation>
</author>
<author><name sortKey="Wu, Yingliang" sort="Wu, Yingliang" uniqKey="Wu Y" first="Yingliang" last="Wu">Yingliang Wu</name>
</author>
<author><name sortKey="Cao, Zhijian" sort="Cao, Zhijian" uniqKey="Cao Z" first="Zhijian" last="Cao">Zhijian Cao</name>
</author>
<author><name sortKey="Xu, Xiuling" sort="Xu, Xiuling" uniqKey="Xu X" first="Xiuling" last="Xu">Xiuling Xu</name>
</author>
<author><name sortKey="Wu, Wenlan" sort="Wu, Wenlan" uniqKey="Wu W" first="Wenlan" last="Wu">Wenlan Wu</name>
</author>
<author><name sortKey="Jiang, Dahe" sort="Jiang, Dahe" uniqKey="Jiang D" first="Dahe" last="Jiang">Dahe Jiang</name>
</author>
<author><name sortKey="Mao, Xin" sort="Mao, Xin" uniqKey="Mao X" first="Xin" last="Mao">Xin Mao</name>
</author>
<author><name sortKey="Liu, Hui" sort="Liu, Hui" uniqKey="Liu H" first="Hui" last="Liu">Hui Liu</name>
</author>
<author><name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name>
</author>
<author><name sortKey="Gong, Rui" sort="Gong, Rui" uniqKey="Gong R" first="Rui" last="Gong">Rui Gong</name>
</author>
<author><name sortKey="Li, Wenxin" sort="Li, Wenxin" uniqKey="Li W" first="Wenxin" last="Li">Wenxin Li</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:16916786</idno>
<idno type="pmid">16916786</idno>
<idno type="doi">10.1080/15216540600820974</idno>
<idno type="wicri:Area/PubMed/Corpus">002119</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002119</idno>
<idno type="wicri:Area/PubMed/Curation">002119</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002119</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002333</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002333</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.</title>
<author><name sortKey="Sha, Yonggang" sort="Sha, Yonggang" uniqKey="Sha Y" first="Yonggang" last="Sha">Yonggang Sha</name>
<affiliation wicri:level="4"><nlm:affiliation>State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan</wicri:regionArea>
<placeName><settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
<orgName type="university">Université de Wuhan</orgName>
</affiliation>
</author>
<author><name sortKey="Wu, Yingliang" sort="Wu, Yingliang" uniqKey="Wu Y" first="Yingliang" last="Wu">Yingliang Wu</name>
</author>
<author><name sortKey="Cao, Zhijian" sort="Cao, Zhijian" uniqKey="Cao Z" first="Zhijian" last="Cao">Zhijian Cao</name>
</author>
<author><name sortKey="Xu, Xiuling" sort="Xu, Xiuling" uniqKey="Xu X" first="Xiuling" last="Xu">Xiuling Xu</name>
</author>
<author><name sortKey="Wu, Wenlan" sort="Wu, Wenlan" uniqKey="Wu W" first="Wenlan" last="Wu">Wenlan Wu</name>
</author>
<author><name sortKey="Jiang, Dahe" sort="Jiang, Dahe" uniqKey="Jiang D" first="Dahe" last="Jiang">Dahe Jiang</name>
</author>
<author><name sortKey="Mao, Xin" sort="Mao, Xin" uniqKey="Mao X" first="Xin" last="Mao">Xin Mao</name>
</author>
<author><name sortKey="Liu, Hui" sort="Liu, Hui" uniqKey="Liu H" first="Hui" last="Liu">Hui Liu</name>
</author>
<author><name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name>
</author>
<author><name sortKey="Gong, Rui" sort="Gong, Rui" uniqKey="Gong R" first="Rui" last="Gong">Rui Gong</name>
</author>
<author><name sortKey="Li, Wenxin" sort="Li, Wenxin" uniqKey="Li W" first="Wenxin" last="Li">Wenxin Li</name>
</author>
</analytic>
<series><title level="j">IUBMB life</title>
<idno type="ISSN">1521-6543</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Biological Assay</term>
<term>COS Cells</term>
<term>Cell Fusion</term>
<term>Chlorocebus aethiops</term>
<term>Coculture Techniques</term>
<term>Escherichia coli (genetics)</term>
<term>Fluorescent Dyes (metabolism)</term>
<term>Glutathione Transferase (metabolism)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Luminescent Proteins (metabolism)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Peptides (chemistry)</term>
<term>Peptides (metabolism)</term>
<term>Peptidyl-Dipeptidase A (metabolism)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (metabolism)</term>
<term>Severe Acute Respiratory Syndrome (metabolism)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Time Factors</term>
<term>Trypsin (pharmacology)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules COS</term>
<term>Colorants fluorescents (métabolisme)</term>
<term>Dosage biologique</term>
<term>Escherichia coli (génétique)</term>
<term>Facteurs temps</term>
<term>Fusion cellulaire</term>
<term>Glutathione transferase (métabolisme)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Peptides ()</term>
<term>Peptides (métabolisme)</term>
<term>Peptidyl-Dipeptidase A (métabolisme)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines luminescentes (métabolisme)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Syndrome respiratoire aigu sévère (métabolisme)</term>
<term>Techniques de coculture</term>
<term>Trypsine (pharmacologie)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fluorescent Dyes</term>
<term>Glutathione Transferase</term>
<term>Green Fluorescent Proteins</term>
<term>Luminescent Proteins</term>
<term>Membrane Glycoproteins</term>
<term>Peptides</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Colorants fluorescents</term>
<term>Glutathione transferase</term>
<term>Glycoprotéines membranaires</term>
<term>Peptides</term>
<term>Peptidyl-Dipeptidase A</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines luminescentes</term>
<term>Protéines recombinantes</term>
<term>Protéines à fluorescence verte</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Trypsine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Trypsin</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Biological Assay</term>
<term>COS Cells</term>
<term>Cell Fusion</term>
<term>Chlorocebus aethiops</term>
<term>Coculture Techniques</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules COS</term>
<term>Dosage biologique</term>
<term>Facteurs temps</term>
<term>Fusion cellulaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Peptides</term>
<term>Techniques de coculture</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">SARS-CoV spike (S) protein-mediated cell fusion is important for the viral entry mechanism and identification of SARS-CoV entry inhibitors. In order to avoid the high risks involved in handling SARS-CoV and to facilitate the study of viral fusion mechanism, we established the cell lines: SR-COS7 cells that stably express both SARS-CoV S protein and red fluorescence protein, R-COS7 cells that stably express red fluorescence protein, and AG-COS7 cells that stably express both ACE2 and green fluorescence protein, respectively. When SR-COS7 cells or R-COS7 cells were cocultured with AG-COS7 cells, syncytia with yellow fluorescence were conveniently observed after 12 h in SR-COS7 cells plus AG-COS7 cells, but not in R-COS7 cells plus AG-COS7 cells. The cell-to-cell fusion efficiency was simply determined for quantitative analysis based on the number of syncytium detected by flow cytometry. Such new cell-to-cell fusion model was further assessed by the potent HR2 peptide inhibitor, which led to the obvious decrease of the cell-to-cell fusion efficiency. The successful fusion and inhibition of cell-based binding assay shows that it can be well used for the study of SARS-CoV entry and inhibition.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16916786</PMID>
<DateCompleted><Year>2006</Year>
<Month>10</Month>
<Day>26</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1521-6543</ISSN>
<JournalIssue CitedMedium="Print"><Volume>58</Volume>
<Issue>8</Issue>
<PubDate><Year>2006</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>IUBMB life</Title>
<ISOAbbreviation>IUBMB Life</ISOAbbreviation>
</Journal>
<ArticleTitle>A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.</ArticleTitle>
<Pagination><MedlinePgn>480-6</MedlinePgn>
</Pagination>
<Abstract><AbstractText>SARS-CoV spike (S) protein-mediated cell fusion is important for the viral entry mechanism and identification of SARS-CoV entry inhibitors. In order to avoid the high risks involved in handling SARS-CoV and to facilitate the study of viral fusion mechanism, we established the cell lines: SR-COS7 cells that stably express both SARS-CoV S protein and red fluorescence protein, R-COS7 cells that stably express red fluorescence protein, and AG-COS7 cells that stably express both ACE2 and green fluorescence protein, respectively. When SR-COS7 cells or R-COS7 cells were cocultured with AG-COS7 cells, syncytia with yellow fluorescence were conveniently observed after 12 h in SR-COS7 cells plus AG-COS7 cells, but not in R-COS7 cells plus AG-COS7 cells. The cell-to-cell fusion efficiency was simply determined for quantitative analysis based on the number of syncytium detected by flow cytometry. Such new cell-to-cell fusion model was further assessed by the potent HR2 peptide inhibitor, which led to the obvious decrease of the cell-to-cell fusion efficiency. The successful fusion and inhibition of cell-based binding assay shows that it can be well used for the study of SARS-CoV entry and inhibition.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sha</LastName>
<ForeName>YongGang</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>YingLiang</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y"><LastName>Cao</LastName>
<ForeName>ZhiJian</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>XiuLing</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>WenLan</ForeName>
<Initials>W</Initials>
</Author>
<Author ValidYN="Y"><LastName>Jiang</LastName>
<ForeName>DaHe</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Mao</LastName>
<ForeName>Xin</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Hui</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Ying</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gong</LastName>
<ForeName>Rui</ForeName>
<Initials>R</Initials>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>WenXin</ForeName>
<Initials>W</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>IUBMB Life</MedlineTA>
<NlmUniqueID>100888706</NlmUniqueID>
<ISSNLinking>1521-6543</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005456">Fluorescent Dyes</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578553">MHV surface projection glycoprotein</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010455">Peptides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C413662">red fluorescent protein</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>147336-22-9</RegistryNumber>
<NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber>
<NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.15.1</RegistryNumber>
<NameOfSubstance UI="D007703">Peptidyl-Dipeptidase A</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.17.-</RegistryNumber>
<NameOfSubstance UI="C413524">angiotensin converting enzyme 2</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.4.21.4</RegistryNumber>
<NameOfSubstance UI="D014357">Trypsin</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001681" MajorTopicYN="Y">Biological Assay</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019556" MajorTopicYN="N">COS Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002459" MajorTopicYN="N">Cell Fusion</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018920" MajorTopicYN="N">Coculture Techniques</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005456" MajorTopicYN="N">Fluorescent Dyes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005982" MajorTopicYN="N">Glutathione Transferase</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007703" MajorTopicYN="N">Peptidyl-Dipeptidase A</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014357" MajorTopicYN="N">Trypsin</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>8</Month>
<Day>19</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2006</Year>
<Month>10</Month>
<Day>27</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
<Month>8</Month>
<Day>19</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16916786</ArticleId>
<ArticleId IdType="pii">H67831954412XQ2U</ArticleId>
<ArticleId IdType="doi">10.1080/15216540600820974</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Hubei</li>
</region>
<settlement><li>Wuhan</li>
</settlement>
<orgName><li>Université de Wuhan</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Cao, Zhijian" sort="Cao, Zhijian" uniqKey="Cao Z" first="Zhijian" last="Cao">Zhijian Cao</name>
<name sortKey="Gong, Rui" sort="Gong, Rui" uniqKey="Gong R" first="Rui" last="Gong">Rui Gong</name>
<name sortKey="Jiang, Dahe" sort="Jiang, Dahe" uniqKey="Jiang D" first="Dahe" last="Jiang">Dahe Jiang</name>
<name sortKey="Li, Wenxin" sort="Li, Wenxin" uniqKey="Li W" first="Wenxin" last="Li">Wenxin Li</name>
<name sortKey="Liu, Hui" sort="Liu, Hui" uniqKey="Liu H" first="Hui" last="Liu">Hui Liu</name>
<name sortKey="Mao, Xin" sort="Mao, Xin" uniqKey="Mao X" first="Xin" last="Mao">Xin Mao</name>
<name sortKey="Wu, Wenlan" sort="Wu, Wenlan" uniqKey="Wu W" first="Wenlan" last="Wu">Wenlan Wu</name>
<name sortKey="Wu, Yingliang" sort="Wu, Yingliang" uniqKey="Wu Y" first="Yingliang" last="Wu">Yingliang Wu</name>
<name sortKey="Xu, Xiuling" sort="Xu, Xiuling" uniqKey="Xu X" first="Xiuling" last="Xu">Xiuling Xu</name>
<name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name>
</noCountry>
<country name="République populaire de Chine"><region name="Hubei"><name sortKey="Sha, Yonggang" sort="Sha, Yonggang" uniqKey="Sha Y" first="Yonggang" last="Sha">Yonggang Sha</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/PubMed/Checkpoint

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002333 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 002333 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    PubMed
   |étape=   Checkpoint
   |type=    RBID
   |clé=     pubmed:16916786
   |texte=   A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i   -Sk "pubmed:16916786" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.

A convenient cell fusion assay for the study of SARS-CoV entry and inhibition.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki