SrasV1, PubMed, Checkpoint, bibRecord, 002323

A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents.

Identifieur interne : 002323 ( PubMed/Checkpoint ); précédent : 002322; suivant : 002324

A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents.

Auteurs : Greg Smith [Australie] ; Ina Smith ; Bruce Harrower ; David Warrilow ; Cheryl Bletchly

Source :

Journal of virological methods [ 0166-0934 ] ; 2006.

RBID : pubmed:16677717

Descripteurs français

KwdFr :
- Humains, RT-PCR (), Virus du SRAS (isolement et purification).
MESH :
- isolement et purification : Virus du SRAS.
- Humains, RT-PCR.

English descriptors

KwdEn :
- Humans, Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification).
MESH :
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- Humans.

Abstract

Medical and veterinary diagnostic and public health laboratories world-wide are increasingly being called upon to introduce molecular diagnostic tests for both endemic and exotic diseases. This demand has accelerated following increasing terrorism fears. Ironically these same concerns have lead to tightening of both import and export controls preventing many laboratories, particularly those outside of the United States, from gaining access to positive control material. This in turn has prevented many laboratories from introducing much needed molecular diagnostic tests. We describe here a generic approach for preparing synthetic DNA or RNA control material for use in either TaqMan or conventional PCR assays. The production of synthetic controls using this approach does not require cloning or special equipment or facilities beyond that found in any laboratory performing molecular diagnostics. The approach significantly reduces the possibility of contamination or erroneously reporting false-positive reactions due to contamination from positive control material. Synthetic controls produced using this approach have been employed in all molecular diagnostic tests performed in our laboratory and are used irrespective of whether we possess the organism or not.

DOI: 10.1016/j.jviromet.2006.03.007
PubMed: 16677717

Affiliations:

Australie

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pubmed:16677717

Le document en format XML

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<author><name sortKey="Smith, Greg" sort="Smith, Greg" uniqKey="Smith G" first="Greg" last="Smith">Greg Smith</name>
<affiliation wicri:level="1"><nlm:affiliation>Public Health Virology, Centre for Public Health Sciences, Queensland Health Scientific Services, 39 Kessels Road, Coopers Plains, Brisbane, Qld 4108, Australia. greg_smith@health.qld.gov.au</nlm:affiliation>
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<author><name sortKey="Smith, Ina" sort="Smith, Ina" uniqKey="Smith I" first="Ina" last="Smith">Ina Smith</name>
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<front><div type="abstract" xml:lang="en">Medical and veterinary diagnostic and public health laboratories world-wide are increasingly being called upon to introduce molecular diagnostic tests for both endemic and exotic diseases. This demand has accelerated following increasing terrorism fears. Ironically these same concerns have lead to tightening of both import and export controls preventing many laboratories, particularly those outside of the United States, from gaining access to positive control material. This in turn has prevented many laboratories from introducing much needed molecular diagnostic tests. We describe here a generic approach for preparing synthetic DNA or RNA control material for use in either TaqMan or conventional PCR assays. The production of synthetic controls using this approach does not require cloning or special equipment or facilities beyond that found in any laboratory performing molecular diagnostics. The approach significantly reduces the possibility of contamination or erroneously reporting false-positive reactions due to contamination from positive control material. Synthetic controls produced using this approach have been employed in all molecular diagnostic tests performed in our laboratory and are used irrespective of whether we possess the organism or not.</div>
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A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents.

A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki