Baculovirus surface display of SARS coronavirus (SARS-CoV) spike protein and immunogenicity of the displayed protein in mice models.
Identifieur interne : 002279 ( PubMed/Checkpoint ); précédent : 002278; suivant : 002280Baculovirus surface display of SARS coronavirus (SARS-CoV) spike protein and immunogenicity of the displayed protein in mice models.
Auteurs : Qian Feng [République populaire de Chine] ; Yingying Liu ; Xiuxia Qu ; Hongkui Deng ; Mingxiao Ding ; Terence L T. Lau ; Albert Cheung-Hoi Yu ; Jianguo ChenSource :
- DNA and cell biology [ 1044-5498 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Antigènes de surface (immunologie), Antigènes de surface (métabolisme), Antigènes viraux (immunologie), Antigènes viraux (métabolisme), Baculoviridae (immunologie), Baculoviridae (métabolisme), Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Organismes génétiquement modifiés, Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Souris, Souris de lignée BALB C, Spodoptera (virologie), Virus du SRAS (immunologie).
- MESH :
- immunologie : Antigènes de surface, Antigènes viraux, Baculoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS.
- métabolisme : Antigènes de surface, Antigènes viraux, Baculoviridae, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- virologie : Spodoptera.
- Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Organismes génétiquement modifiés, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antigens, Surface (immunology), Antigens, Surface (metabolism), Antigens, Viral (immunology), Antigens, Viral (metabolism), Baculoviridae (immunology), Baculoviridae (metabolism), Female, Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mice, Inbred BALB C, Organisms, Genetically Modified, SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Spodoptera (virology), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , immunology : Antigens, Surface, Antigens, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Antigens, Surface, Antigens, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : Baculoviridae, SARS Virus.
- metabolism : Baculoviridae.
- virology : Spodoptera.
- Animals, Female, Mice, Mice, Inbred BALB C, Organisms, Genetically Modified, Spike Glycoprotein, Coronavirus.
Abstract
The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.
DOI: 10.1089/dna.2006.25.668
PubMed: 17184168
Affiliations:
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pubmed:17184168Le document en format XML
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<front><div type="abstract" xml:lang="en">The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.</div>
</front>
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<country name="République populaire de Chine"><noRegion><name sortKey="Feng, Qian" sort="Feng, Qian" uniqKey="Feng Q" first="Qian" last="Feng">Qian Feng</name>
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