Construction of a severe acute respiratory syndrome coronavirus infectious cDNA clone and a replicon to study coronavirus RNA synthesis.
Identifieur interne : 002249 ( PubMed/Checkpoint ); précédent : 002248; suivant : 002250Construction of a severe acute respiratory syndrome coronavirus infectious cDNA clone and a replicon to study coronavirus RNA synthesis.
Auteurs : Fernando Almazán [Espagne] ; Marta L. Dediego ; Carmen Galán ; David Escors ; Enrique Alvarez ; Javier Ortego ; Isabel Sola ; Sonia Zu Iga ; Sara Alonso ; Jose L. Moreno ; Aitor Nogales ; Carmen Capiscol ; Luis EnjuanesSource :
- Journal of virology [ 0022-538X ] ; 2006.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN viral (génétique), ARN viral (biosynthèse), ARN viral (génétique), Animaux, Cellules Vero, Chromosomes artificiels de bactérie (génétique), Clonage moléculaire, Cricetinae, Données de séquences moléculaires, Humains, Lignée cellulaire, Réplicon, Séquence nucléotidique, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- biosynthèse : ARN viral.
- génétique : ADN complémentaire, ADN viral, ARN viral, Chromosomes artificiels de bactérie, Virus du SRAS.
- métabolisme : Virus du SRAS.
- Animaux, Cellules Vero, Clonage moléculaire, Cricetinae, Données de séquences moléculaires, Humains, Lignée cellulaire, Réplicon, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Chromosomes, Artificial, Bacterial (genetics), Cloning, Molecular, Cricetinae, DNA, Complementary (genetics), DNA, Viral (genetics), Humans, Molecular Sequence Data, RNA, Viral (biosynthesis), RNA, Viral (genetics), Replicon, SARS Virus (genetics), SARS Virus (metabolism), Vero Cells.
- MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , genetics : DNA, Complementary, DNA, Viral, RNA, Viral.
- genetics : Chromosomes, Artificial, Bacterial, SARS Virus.
- metabolism : SARS Virus.
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Cloning, Molecular, Cricetinae, Humans, Molecular Sequence Data, Replicon, Vero Cells.
Abstract
The engineering of a full-length infectious cDNA clone and a functional replicon of the severe acute respiratory syndrome coronavirus (SARS-CoV) Urbani strain as bacterial artificial chromosomes (BACs) is described in this study. In this system, the viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and further amplified in the cytoplasm by the viral replicase. Both the infectious clone and the replicon were fully stable in Escherichia coli. Using the SARS-CoV replicon, we have shown that the recently described RNA-processing enzymes exoribonuclease, endoribonuclease, and 2'-O-ribose methyltransferase were essential for efficient coronavirus RNA synthesis. The SARS reverse genetic system developed as a BAC constitutes a useful tool for the study of fundamental viral processes and also for developing genetically defined vaccines.
DOI: 10.1128/JVI.00385-06
PubMed: 16928748
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:16928748Le document en format XML
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In this system, the viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and further amplified in the cytoplasm by the viral replicase. Both the infectious clone and the replicon were fully stable in Escherichia coli. Using the SARS-CoV replicon, we have shown that the recently described RNA-processing enzymes exoribonuclease, endoribonuclease, and 2'-O-ribose methyltransferase were essential for efficient coronavirus RNA synthesis. The SARS reverse genetic system developed as a BAC constitutes a useful tool for the study of fundamental viral processes and also for developing genetically defined vaccines.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16928748</PMID><DateCompleted><Year>2006</Year><Month>12</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0022-538X</ISSN><JournalIssue CitedMedium="Print"><Volume>80</Volume><Issue>21</Issue><PubDate><Year>2006</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of virology</Title><ISOAbbreviation>J. Virol.</ISOAbbreviation></Journal><ArticleTitle>Construction of a severe acute respiratory syndrome coronavirus infectious cDNA clone and a replicon to study coronavirus RNA synthesis.</ArticleTitle><Pagination><MedlinePgn>10900-6</MedlinePgn></Pagination><Abstract><AbstractText>The engineering of a full-length infectious cDNA clone and a functional replicon of the severe acute respiratory syndrome coronavirus (SARS-CoV) Urbani strain as bacterial artificial chromosomes (BACs) is described in this study. In this system, the viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and further amplified in the cytoplasm by the viral replicase. Both the infectious clone and the replicon were fully stable in Escherichia coli. Using the SARS-CoV replicon, we have shown that the recently described RNA-processing enzymes exoribonuclease, endoribonuclease, and 2'-O-ribose methyltransferase were essential for efficient coronavirus RNA synthesis. The SARS reverse genetic system developed as a BAC constitutes a useful tool for the study of fundamental viral processes and also for developing genetically defined vaccines.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Almazán</LastName><ForeName>Fernando</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, CSIC, Darwin 3, Campus Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dediego</LastName><ForeName>Marta L</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Galán</LastName><ForeName>Carmen</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Escors</LastName><ForeName>David</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Alvarez</LastName><ForeName>Enrique</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Ortego</LastName><ForeName>Javier</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Sola</LastName><ForeName>Isabel</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Zuñiga</LastName><ForeName>Sonia</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Alonso</LastName><ForeName>Sara</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Moreno</LastName><ForeName>Jose L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Nogales</LastName><ForeName>Aitor</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Capiscol</LastName><ForeName>Carmen</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Enjuanes</LastName><ForeName>Luis</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AY278741</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>08</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Virol</MedlineTA><NlmUniqueID>0113724</NlmUniqueID><ISSNLinking>0022-538X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D022202" MajorTopicYN="N">Chromosomes, Artificial, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012093" MajorTopicYN="N">Replicon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName 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Madrid</li></region></list><tree><noCountry><name sortKey="Alonso, Sara" sort="Alonso, Sara" uniqKey="Alonso S" first="Sara" last="Alonso">Sara Alonso</name><name sortKey="Alvarez, Enrique" sort="Alvarez, Enrique" uniqKey="Alvarez E" first="Enrique" last="Alvarez">Enrique Alvarez</name><name sortKey="Capiscol, Carmen" sort="Capiscol, Carmen" uniqKey="Capiscol C" first="Carmen" last="Capiscol">Carmen Capiscol</name><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><name sortKey="Escors, David" sort="Escors, David" uniqKey="Escors D" first="David" last="Escors">David Escors</name><name sortKey="Galan, Carmen" sort="Galan, Carmen" uniqKey="Galan C" first="Carmen" last="Galán">Carmen Galán</name><name sortKey="Moreno, Jose L" sort="Moreno, Jose L" uniqKey="Moreno J" first="Jose L" last="Moreno">Jose L. Moreno</name><name sortKey="Nogales, Aitor" sort="Nogales, Aitor" uniqKey="Nogales A" first="Aitor" last="Nogales">Aitor Nogales</name><name sortKey="Ortego, Javier" sort="Ortego, Javier" uniqKey="Ortego J" first="Javier" last="Ortego">Javier Ortego</name><name sortKey="Sola, Isabel" sort="Sola, Isabel" uniqKey="Sola I" first="Isabel" last="Sola">Isabel Sola</name><name sortKey="Zu Iga, Sonia" sort="Zu Iga, Sonia" uniqKey="Zu Iga S" first="Sonia" last="Zu Iga">Sonia Zu Iga</name></noCountry><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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