SrasV1, PubMed, Checkpoint, bibRecord, 002152

Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.

Identifieur interne : 002152 ( PubMed/Checkpoint ); précédent : 002151; suivant : 002153

Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.

Auteurs : Bo Zhao [République populaire de Chine] ; Ning-Yi Jin ; Rui-Lin Wang ; Li-Shu Zhang ; Ying-Jiu Zhang

Source :

Viral immunology [ 0882-8245 ] ; 2006.

RBID : pubmed:16987069

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral, Base Sequence, CD4-Positive T-Lymphocytes (immunology), CD8-Positive T-Lymphocytes (immunology), Cell Line, Cricetinae, Female, Immunization, Immunoglobulin G (blood), Membrane Glycoproteins (administration & dosage), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Molecular Sequence Data, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, T-Lymphocytes, Cytotoxic (immunology), Vaccines, DNA (administration & dosage), Vaccines, DNA (immunology), Viral Envelope Proteins (administration & dosage), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
MESH :
- chemical , administration & dosage : Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical , blood : Immunoglobulin G.
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Vaccines, DNA, Viral Envelope Proteins.
- chemical : Antibodies, Viral, Spike Glycoprotein, Coronavirus.
- immunology : CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Cytotoxic.
- prevention & control : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cricetinae, Female, Immunization, Mice, Mice, Inbred BALB C, Molecular Sequence Data.

Abstract

According to data in GenBank, a gene encoding SARS spike protein fragment 1 (S1) was synthesized. After recombination with an immunostimulatory sequence (ISS), the gene was cloned into the plasmid pIRES to produce pIRES-ISS-S1. On confirmation of the expression of S1 protein by indirect immunofluorescence assay (IFA), after the transfection of pIRES-ISS-S1 into BHK-21 cells, the DNA vaccine was repeatedly administrated to BALB/c mice. CD4+ and CD8+ spleen T lymphocytes were analyzed by flow cytometry (FCM) to evaluate T cell-mediated immune responses, the antigen-specific responses of T cells were evaluated by cytotoxic T lymphocyte (CTL) assay, and the level of IgG in antisera from immunized mice was determined by enzyme-linked immunosorbent assay. Results showed that the counts of spleen CD4+ and CD8+ T lymphocytes were increased, that the T cell-mediated immune responses showed antigen specificity, and that IgG was significantly induced with DNA vaccines pIRES-ISS-S1 and pIRES-S1 at titers of 1:320 and 1:160, respectively. These results are promising for the protective immunization of humans.

DOI: 10.1089/vim.2006.19.518
PubMed: 16987069

Affiliations:

République populaire de Chine

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Le document en format XML

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<term>CD8-Positive T-Lymphocytes (immunology)</term>
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<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
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<term>Vaccines, DNA (immunology)</term>
<term>Viral Envelope Proteins (administration & dosage)</term>
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<term>Viral Envelope Proteins (immunology)</term>
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<term>Glycoprotéines membranaires (administration et posologie)</term>
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<front><div type="abstract" xml:lang="en">According to data in GenBank, a gene encoding SARS spike protein fragment 1 (S1) was synthesized. After recombination with an immunostimulatory sequence (ISS), the gene was cloned into the plasmid pIRES to produce pIRES-ISS-S1. On confirmation of the expression of S1 protein by indirect immunofluorescence assay (IFA), after the transfection of pIRES-ISS-S1 into BHK-21 cells, the DNA vaccine was repeatedly administrated to BALB/c mice. CD4+ and CD8+ spleen T lymphocytes were analyzed by flow cytometry (FCM) to evaluate T cell-mediated immune responses, the antigen-specific responses of T cells were evaluated by cytotoxic T lymphocyte (CTL) assay, and the level of IgG in antisera from immunized mice was determined by enzyme-linked immunosorbent assay. Results showed that the counts of spleen CD4+ and CD8+ T lymphocytes were increased, that the T cell-mediated immune responses showed antigen specificity, and that IgG was significantly induced with DNA vaccines pIRES-ISS-S1 and pIRES-S1 at titers of 1:320 and 1:160, respectively. These results are promising for the protective immunization of humans.</div>
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<Abstract><AbstractText>According to data in GenBank, a gene encoding SARS spike protein fragment 1 (S1) was synthesized. After recombination with an immunostimulatory sequence (ISS), the gene was cloned into the plasmid pIRES to produce pIRES-ISS-S1. On confirmation of the expression of S1 protein by indirect immunofluorescence assay (IFA), after the transfection of pIRES-ISS-S1 into BHK-21 cells, the DNA vaccine was repeatedly administrated to BALB/c mice. CD4+ and CD8+ spleen T lymphocytes were analyzed by flow cytometry (FCM) to evaluate T cell-mediated immune responses, the antigen-specific responses of T cells were evaluated by cytotoxic T lymphocyte (CTL) assay, and the level of IgG in antisera from immunized mice was determined by enzyme-linked immunosorbent assay. Results showed that the counts of spleen CD4+ and CD8+ T lymphocytes were increased, that the T cell-mediated immune responses showed antigen specificity, and that IgG was significantly induced with DNA vaccines pIRES-ISS-S1 and pIRES-S1 at titers of 1:320 and 1:160, respectively. These results are promising for the protective immunization of humans.</AbstractText>
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<name sortKey="Wang, Rui Lin" sort="Wang, Rui Lin" uniqKey="Wang R" first="Rui-Lin" last="Wang">Rui-Lin Wang</name>
<name sortKey="Zhang, Li Shu" sort="Zhang, Li Shu" uniqKey="Zhang L" first="Li-Shu" last="Zhang">Li-Shu Zhang</name>
<name sortKey="Zhang, Ying Jiu" sort="Zhang, Ying Jiu" uniqKey="Zhang Y" first="Ying-Jiu" last="Zhang">Ying-Jiu Zhang</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Bo" sort="Zhao, Bo" uniqKey="Zhao B" first="Bo" last="Zhao">Bo Zhao</name>
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   |texte=   Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.
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       | NlmPubMed2Wicri -a SrasV1

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Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.

Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.

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