Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells.
Identifieur interne : 002077 ( PubMed/Checkpoint ); précédent : 002076; suivant : 002078Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells.
Auteurs : Sehaam Khan [Singapour] ; Burtram C. Fielding ; Timothy H P. Tan ; Chih-Fong Chou ; Shuo Shen ; Seng Gee Lim ; Wanjin Hong ; Yee-Joo TanSource :
- Virus research [ 0168-1702 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Apoptose, Cellules Vero, Délétion de séquence, Facteurs temps, Fragmentation de l'ADN, Microscopie de fluorescence, Noyau de la cellule (), Nucléole (), Nécrose, Protéine Bax (physiologie), Protéines virales non structurales (génétique), Protéines virales non structurales (métabolisme), Protéines virales non structurales (physiologie), Virus du SRAS (pathogénicité).
- MESH :
- génétique : Protéines virales non structurales.
- métabolisme : Protéines virales non structurales.
- pathogénicité : Virus du SRAS.
- physiologie : Protéine Bax, Protéines virales non structurales.
- Animaux, Apoptose, Cellules Vero, Délétion de séquence, Facteurs temps, Fragmentation de l'ADN, Microscopie de fluorescence, Noyau de la cellule, Nucléole, Nécrose.
English descriptors
- KwdEn :
- Animals, Apoptosis, Cell Nucleolus (chemistry), Cell Nucleus (chemistry), Chlorocebus aethiops, DNA Fragmentation, Microscopy, Fluorescence, Necrosis, SARS Virus (pathogenicity), Sequence Deletion, Time Factors, Vero Cells, Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Viral Nonstructural Proteins (physiology), bcl-2-Associated X Protein (physiology).
- MESH :
- chemical , genetics : Viral Nonstructural Proteins.
- chemistry : Cell Nucleolus, Cell Nucleus.
- chemical , metabolism : Viral Nonstructural Proteins.
- pathogenicity : SARS Virus.
- chemical , physiology : Viral Nonstructural Proteins, bcl-2-Associated X Protein.
- Animals, Apoptosis, Chlorocebus aethiops, DNA Fragmentation, Microscopy, Fluorescence, Necrosis, Sequence Deletion, Time Factors, Vero Cells.
Abstract
The genome of the severe acute respiratory syndrome coronavirus encodes for eight accessory viral proteins with no known homologues in other coronaviruses. One of these is the 3b protein, which is encoded by the second open reading frame in subgenomic RNA 3 and contains 154 amino acids. Here, a detailed time-course study was performed to compare the apoptosis and necrosis profiles induced by full-length 3b, a 3b mutant that was deleted by 30 amino acids from the C terminus (3bDelta124-154) and the classical apoptosis inducer, Bax. Our results showed that Vero E6 cells transfected with a construct for expressing 3b underwent necrosis as early as 6h after transfection and underwent simultaneous necrosis and apoptosis at later time-points. At all the time-points analysed, the apoptosis induced by the expression of 3b was less than the level induced by Bax but the level of necrosis was comparable. The 3bDelta124-154 mutant behaves in a similar manner indicating that the localization of the 3b protein does not seems to be important for the cell-death pathways since full-length 3b is localized predominantly to the nucleolus, while the mutant is found to be concentrated in the peri-nuclear regions. To our knowledge, this is the first report of the induction of necrosis by a SARS-CoV protein.
DOI: 10.1016/j.virusres.2006.06.005
PubMed: 16965829
Affiliations:
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Fielding</name></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H P" last="Tan">Timothy H P. Tan</name></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Nucleolus (chemistry)</term><term>Cell Nucleus (chemistry)</term><term>Chlorocebus aethiops</term><term>DNA Fragmentation</term><term>Microscopy, Fluorescence</term><term>Necrosis</term><term>SARS Virus (pathogenicity)</term><term>Sequence Deletion</term><term>Time Factors</term><term>Vero Cells</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Viral Nonstructural Proteins (physiology)</term><term>bcl-2-Associated X Protein (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Délétion de séquence</term><term>Facteurs temps</term><term>Fragmentation de l'ADN</term><term>Microscopie de fluorescence</term><term>Noyau de la cellule ()</term><term>Nucléole ()</term><term>Nécrose</term><term>Protéine Bax (physiologie)</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales non structurales (métabolisme)</term><term>Protéines virales non structurales (physiologie)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Nucleolus</term><term>Cell Nucleus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéine Bax</term><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Viral Nonstructural Proteins</term><term>bcl-2-Associated X Protein</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Chlorocebus aethiops</term><term>DNA Fragmentation</term><term>Microscopy, Fluorescence</term><term>Necrosis</term><term>Sequence Deletion</term><term>Time Factors</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Délétion de séquence</term><term>Facteurs temps</term><term>Fragmentation de l'ADN</term><term>Microscopie de fluorescence</term><term>Noyau de la cellule</term><term>Nucléole</term><term>Nécrose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The genome of the severe acute respiratory syndrome coronavirus encodes for eight accessory viral proteins with no known homologues in other coronaviruses. One of these is the 3b protein, which is encoded by the second open reading frame in subgenomic RNA 3 and contains 154 amino acids. Here, a detailed time-course study was performed to compare the apoptosis and necrosis profiles induced by full-length 3b, a 3b mutant that was deleted by 30 amino acids from the C terminus (3bDelta124-154) and the classical apoptosis inducer, Bax. Our results showed that Vero E6 cells transfected with a construct for expressing 3b underwent necrosis as early as 6h after transfection and underwent simultaneous necrosis and apoptosis at later time-points. At all the time-points analysed, the apoptosis induced by the expression of 3b was less than the level induced by Bax but the level of necrosis was comparable. The 3bDelta124-154 mutant behaves in a similar manner indicating that the localization of the 3b protein does not seems to be important for the cell-death pathways since full-length 3b is localized predominantly to the nucleolus, while the mutant is found to be concentrated in the peri-nuclear regions. To our knowledge, this is the first report of the induction of necrosis by a SARS-CoV protein.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16965829</PMID><DateCompleted><Year>2007</Year><Month>03</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0168-1702</ISSN><JournalIssue CitedMedium="Print"><Volume>122</Volume><Issue>1-2</Issue><PubDate><Year>2006</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Virus research</Title><ISOAbbreviation>Virus Res.</ISOAbbreviation></Journal><ArticleTitle>Over-expression of severe acute respiratory syndrome coronavirus 3b protein induces both apoptosis and necrosis in Vero E6 cells.</ArticleTitle><Pagination><MedlinePgn>20-7</MedlinePgn></Pagination><Abstract><AbstractText>The genome of the severe acute respiratory syndrome coronavirus encodes for eight accessory viral proteins with no known homologues in other coronaviruses. One of these is the 3b protein, which is encoded by the second open reading frame in subgenomic RNA 3 and contains 154 amino acids. Here, a detailed time-course study was performed to compare the apoptosis and necrosis profiles induced by full-length 3b, a 3b mutant that was deleted by 30 amino acids from the C terminus (3bDelta124-154) and the classical apoptosis inducer, Bax. Our results showed that Vero E6 cells transfected with a construct for expressing 3b underwent necrosis as early as 6h after transfection and underwent simultaneous necrosis and apoptosis at later time-points. At all the time-points analysed, the apoptosis induced by the expression of 3b was less than the level induced by Bax but the level of necrosis was comparable. The 3bDelta124-154 mutant behaves in a similar manner indicating that the localization of the 3b protein does not seems to be important for the cell-death pathways since full-length 3b is localized predominantly to the nucleolus, while the mutant is found to be concentrated in the peri-nuclear regions. To our knowledge, this is the first report of the induction of necrosis by a SARS-CoV protein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Sehaam</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fielding</LastName><ForeName>Burtram C</ForeName><Initials>BC</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Timothy H P</ForeName><Initials>TH</Initials></Author><Author ValidYN="Y"><LastName>Chou</LastName><ForeName>Chih-Fong</ForeName><Initials>CF</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Shuo</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Lim</LastName><ForeName>Seng Gee</ForeName><Initials>SG</Initials></Author><Author ValidYN="Y"><LastName>Hong</LastName><ForeName>Wanjin</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Yee-Joo</ForeName><Initials>YJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>09</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Virus Res</MedlineTA><NlmUniqueID>8410979</NlmUniqueID><ISSNLinking>0168-1702</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017361">Viral Nonstructural Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051028">bcl-2-Associated X Protein</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C118611">glycoprotein 3b, coronavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="Y">Apoptosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002466" MajorTopicYN="N">Cell Nucleolus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053938" MajorTopicYN="N">DNA Fragmentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009336" MajorTopicYN="Y">Necrosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017384" MajorTopicYN="N">Sequence Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051028" MajorTopicYN="N">bcl-2-Associated X Protein</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2006</Year><Month>03</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2006</Year><Month>05</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2006</Year><Month>06</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>9</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>3</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">15096203</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 2005 Feb;65(2):69-78</Citation><ArticleIdList><ArticleId IdType="pubmed">15708633</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mol Biol Cell. 2002 Mar;13(3):978-88</Citation><ArticleIdList><ArticleId IdType="pubmed">11907276</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Immunol. 2002 Nov;3(11):1013-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12407409</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 May;79(9):5833-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15827197</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 May;79(9):5288-95</Citation><ArticleIdList><ArticleId IdType="pubmed">15827143</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Int J Exp Pathol. 2001 Apr;82(2):65-76</Citation><ArticleIdList><ArticleId IdType="pubmed">11454099</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Rev Neurosci. 2003 Aug;4(8):672-84</Citation><ArticleIdList><ArticleId IdType="pubmed">12894242</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Jan;79(1):503-11</Citation><ArticleIdList><ArticleId IdType="pubmed">15596843</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nat Med. 2004 Dec;10(12 Suppl):S88-97</Citation><ArticleIdList><ArticleId IdType="pubmed">15577937</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Dec;79(23):14909-22</Citation><ArticleIdList><ArticleId IdType="pubmed">16282490</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2004 Jul 1;324(2):251-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15207612</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Lancet. 2003 Jul 26;362(9380):263-70</Citation><ArticleIdList><ArticleId IdType="pubmed">12892955</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2003 Aug 29;331(5):991-1004</Citation><ArticleIdList><ArticleId IdType="pubmed">12927536</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Rev Med Virol. 2004 Jul-Aug;14(4):209-16</Citation><ArticleIdList><ArticleId IdType="pubmed">15248249</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Vet Immunol Immunopathol. 2004 Dec 8;102(3):131-42</Citation><ArticleIdList><ArticleId IdType="pubmed">15507300</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Virol. 2004 Jan;29(1):13-22</Citation><ArticleIdList><ArticleId IdType="pubmed">14675864</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Mar;79(5):3182-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15709039</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2002 Jul;83(Pt 7):1547-1564</Citation><ArticleIdList><ArticleId IdType="pubmed">12075073</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 2005 Jul;86(Pt 7):1921-1930</Citation><ArticleIdList><ArticleId IdType="pubmed">15958670</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Exp Cell Res. 2003 Feb 1;283(1):1-16</Citation><ArticleIdList><ArticleId IdType="pubmed">12565815</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Pathol. 2003 Jul;200(3):282-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12845623</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>EMBO Rep. 2002 Jul;3(7):604-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12101090</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Infect Dis. 2005 Oct 15;41(8):1089-96</Citation><ArticleIdList><ArticleId IdType="pubmed">16163626</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2005 Dec;114(1-2):70-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16046244</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Infect Dis. 2004 May 15;38(10):1420-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15156481</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Death Differ. 2001 Feb;8(2):113-26</Citation><ArticleIdList><ArticleId IdType="pubmed">11313713</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>FEBS Lett. 2004 May 7;565(1-3):111-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15135062</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Dec;78(24):14043-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15564512</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 1999 Dec 15;65(2):161-74</Citation><ArticleIdList><ArticleId IdType="pubmed">10581389</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>EMBO J. 2001 Aug 15;20(16):4325-31</Citation><ArticleIdList><ArticleId IdType="pubmed">11500358</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Clin Diagn Lab Immunol. 2004 Mar;11(2):362-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15013989</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Med Virol. 2004 Jul;73(3):323-31</Citation><ArticleIdList><ArticleId IdType="pubmed">15170624</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Pathology. 2003 Dec;35(6):526-31</Citation><ArticleIdList><ArticleId IdType="pubmed">14660106</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Aug;79(15):10083-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16014971</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Mol Biol. 2004 Jul 30;341(1):271-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15312778</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2005 Apr 29;330(1):286-92</Citation><ArticleIdList><ArticleId IdType="pubmed">15781262</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 Jul;78(13):6723-34</Citation><ArticleIdList><ArticleId IdType="pubmed">15194747</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Nature. 2003 May 15;423(6937):240</Citation><ArticleIdList><ArticleId IdType="pubmed">12748632</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Death Differ. 2002 Apr;9(4):367-93</Citation><ArticleIdList><ArticleId IdType="pubmed">11965491</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Arch Pathol Lab Med. 2004 Feb;128(2):195-204</Citation><ArticleIdList><ArticleId IdType="pubmed">14736283</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Pathol. 2005 Jul;206(3):251-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15892035</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Genes Dev. 2004 Jun 1;18(11):1223-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15175258</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cell Cycle. 2004 Jan;3(1):64-6</Citation><ArticleIdList><ArticleId IdType="pubmed">14657668</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virus Res. 2000 Mar;67(1):81-90</Citation><ArticleIdList><ArticleId IdType="pubmed">10773321</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virol J. 2005 Aug 17;2:66</Citation><ArticleIdList><ArticleId IdType="pubmed">16107218</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Mod Pathol. 2005 Nov;18(11):1432-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15920543</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Singapour</li></country></list><tree><noCountry><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C" last="Fielding">Burtram C. Fielding</name><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H P" last="Tan">Timothy H P. Tan</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></noCountry><country name="Singapour"><noRegion><name sortKey="Khan, Sehaam" sort="Khan, Sehaam" uniqKey="Khan S" first="Sehaam" last="Khan">Sehaam Khan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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